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식용식물자원으로부터 활성물질의 탐색-XI -GC를 이용한 상추(Lactuca sativa L.)의 품종별 Phytol 함량 분석-
송명종,이진희,방면호,김지영,정대균,배동근,김인호,백남인,Song, Myoung-Chong,Lee, Jin-Hee,Bang, Myun-Ho,Kim, Ji-Young,Chung, Dae-Kyun,Pai, Tong-Kun,Kim, In-Ho,Baek, Nam-In 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.4
An easy, sensitive and rapid method for the analysis of phytol, a strong ACAT (Acyl-CoA: Cholesterol Acyltransferase) inhibitory diterpenoid, was established. The pre-treatment of sample for GC analysis of phytol includes aq. MeOH extraction, partition of the extracts between EtOAc and water, and filtration of organic phase through Supelclean LC-Si SPE tube. The GC analysis of phytol for a variety of Lactuca sativa was carried out on DB-5 capillary column with flame ionization detector (FID). The consistency in phytol contents according to each variety was not shown. Especially, Jeoktoma, as RS-2 type, showed the highest phytol content.
식용식물자원으로부터 활성물질의 탐색-VI - 쑥갓(Chrysanthemum coronarium L.)으로부터 sterol의 분리 -
송명종,홍윤희,김동현,김대근,정인식,이윤형,김성훈,박미현,김인호,권병옥,백남인,Song, Myoung-Chong,Hong, Yoon-Hee,Kim, Dong-Hyun,Kim, Dae-Keun,Chung, In-Sik,Lee, Youn-Hyung,Kim, Sung-Hoon,Park, Mi-Hyun,Kim, In-Ho,Kwon, Byoung-Mog,Baek, 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.4
쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. EtOAc 분획으로부터 silica gel column chromatography를 반복하여 3종의 sterol을 분리하였다. 각각의 화학구조는, NMR및 MS등의 스펙트럼을 해석하여, $stigmast-5-en-3{\beta}-ol\;(1,\;{\beta}-sitosterol),\;stigmast-4-en-6{\beta}-ol-3-one$ (2), $stigmast-4-en-6{\alpha}-3-one$ (3)으로 동정하였다. 화합물 2와 3은 육상식물에서는 처음으로 분리되었다. The aerial parts of tile Garland (Chrysanthemum comnarium L.) were extracted in MeOH and solvent fractionated with EtOAc, n-BuOH and water, successively. EtOAc fractions gave three steroid compounds through application of silica gel column chromatographies. The chemical structures of the steroids were determined by the interpretation of several spectral data, including NMR and MS as $stigmast-5-en-3{\beta}-ol\;(1,\;{\beta}-sitosterol),\;stigmast-4-en-6{\beta}-ol-3-one$ (2), $stigmast-4-en-6{\alpha}-ol-3-one$ (3). Compounds 2 and 3 have been so far reported only in the aquatic plants, were isolated for the first time from the land plants.
육두구 (Myristica fragrans Houtt)로부터 Phenylpropanoid의 분리
송명종 ( Myoung Chong Song ),안은미 ( Eun Mi Ahn ),방면호 ( Myun Ho Bang ),김세영 ( Se Young Kim ),노영덕 ( Yeong Deok Rho ),권병목 ( Byoung Mog Kwon ),이현선 ( Hyun Sun Lee ),백남인 ( Nam In Baek ) 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.3
식용식물자원으로부터 활성물질의 탐색-6 -GC를 이용한 상추(Lactuca sativa L.)의 품종별 Phytol 함량 분석-
송명종 ( Myoung Chong Song ),이진희 ( Jin Hee Lee ),방면호 ( Myun Ho Bang ),김지영 ( Ji Young Kim ),정대균 ( Dae Kyun Chung ),배동근 ( Tong Kun Pai ),김인호 ( In Ho Kim ),백남인 ( Nam In Baek ) 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.4
산철쭉(Rhododendron yedoense var. poukhanense) 꽃으로부터 Terpenoid의 분리.동정
홍윤희,송명종,한재택,장태오,이윤형,김성훈,김승애,박미현,백남인,Hong, Yoon-Hee,Song, Myoung-Chong,Han, Jae-Taek,Jang, Tae-O,Lee, Youn-Hyung,Kim, Sung-Hoon,Kim, Seung-Ae,Park, Mi-Hyun,Baek, Nam-Ln 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.2
산철쭉 끝을 MeOH로 추출하고, 이를 EtOAc, n-BuOH과 $H_2O$로 분배 추출하였다. EtOAc추출물과 n-BuOH 추출물에 대하여 silica gel과 ODS column을 이용하여 chromatography하거나, 아세틸화 한 후 sillica gel column chromatography를 수행하여 2종의 triterfenoid와 3종의 diterpenoio를 분리하였다. 각 화합물은 $^1H-^lH\;COSY$, HMQC, HMBC와 같은 2D-NMR기법을 포함한 스펙트럼 데이터를 해석하여 $2{\alpha}\;3\{beta}-dihydroxylolean-12-ene (1), ursolic acid (2), grayanotoxin IV (3), grayanotoxin I (4) 및 grayanotoxin III (5) 등으로 동정하였다. Extracts were obtained from the flower of Rhododendro yedoense var. poukhanense. (7 kg) in 80% aqueous MeOH and successively fractionated with solvent of EtOAc, n-BuOH and $H_2O$, successively. Silica gel and ODS column chromatographies of the EtOAc and n-BuOH fractions were repeatedly carried out by using the various solvent systems to give five terpenoids. Chemical structures of the isolated terpenoids were determined as $2{\alpha},3{\beta}-dihydroxylolean-12-ene$ (1), ursolic acid (2), grayanotoxin IV (3), grayanotoxin I (4) and grayanotoxin III (5) based on the interpretation of several spectral data including 2D-NMR such as $^1H-^1H\;COSY$, HMQC and HMBC.
식용식물자원으로부터 활성물질의 탐색-XII. - 꽃마리(Trigonotis peduncularis Benth.)로부터 Flavonol 배당체의 분리 및 hACAT1 저해활성 -
양혜정,송명종,방면호,이진희,정인식,이윤형,정태숙,권병목,김성훈,김대근,박미현,백남인,Yang, Hye-Joung,Song, Myoung-Chong,Bang, Myun-Ho,Lee, Jin-Hee,Chung, In-Sik,Lee, Youn-Hyung,Jeong, Tae-Sook,Kwon, Byoung-Mog,Kim, Sung-Hoon,Kim, Dae-Keu 한국응용생명화학회 2005 Applied Biological Chemistry (Appl Biol Chem) Vol.48 No.1
꽃마리를 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 $H_2O$로 용매 분획하였다. EtOAc와 n-BuOH 분획에 대하여 column chromatography를 반복하여 4종의 flavonol 배당체를 분리하였다. 각각에 대하여 2D-NMR을 포함한 스펙트럼 데이터의 해석과 문헌 자료를 조사하여 $kaempferol-3-O-{\beta}-{D}-glucopyranoside(astragalin),\;kaempferol-3-O-{\alpha}-{L}-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside(nicotiflorin),\;quercetin-3-O-{\alpha}-{L}-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside(rutin),\;quercetin-3-O-{\beta}-{D}-glucopyranoside(isoquercitrin)$로 구조를 결정하였다. 이 화합물들은 꽃마리에서는 이번에 처음 분리, 보고되었다. 또한 $nicotiflorin(100\;{\mu}g/ml)$은 hACAT1에 대하여 $68.3{\pm}1.2%$ 저해활성을 나타내었다. The MeOH extracts obtained from whole plant of Trigonotis peduncularis Benth. were solvent fractionated using EtOAc, n-BuOH and water, successively. The EtOAc and n-BuOH fractions gave four flavonol glycosides through application of silica gel and octadecyl silica gel (ODS) column chromatographies. The chemical structures of the flavonol glycosides were determined by the interpretation of several spectral data including 2D-NMR as $kaempferol-3-O-{\beta}-{D}-glucopyranoside\;(astragalin,\;1),\;kaempferol-3-O-{\alpha}-{L}-rhamnopyranosyl\;(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside\;(nicotiflorin,\;2),\;quercetin-3-O-{\alpha}-{L}-rhamnopyranosyl(1{\rightarrow}6)-{\beta}-{D}-glucopyranoside\;(rutin,\;3),\;quercetin-3-O-{\beta}-{D}-glucopyranoside\;(isoquercitrin,\;4)$. The flavonoids have been first isolated from this plant. Nicotiflorin $(100\;{\mu}g/ml)$ showed $68.3{\pm}1.2%$ of the inhibitory effect on hACAT1(human Acyl CoA: cholesterol transferase 1) activity.
식용식물자원으로부터 활성물질의 탐색-XVII. 산딸나무(Cornus kousa Burg.)의 열매로부터 sterol 화합물의 분리
이대영,송명종,유종수,김성훈,정인식,김대근,박미현,권병목,김세영,백남인,Lee, Dae-Young,Song, Myoung-Chong,Yoo, Jong-Su,Kim, Sung-Hoon,Chung, In-Sik,Kim, Dae-Keun,Park, Mi-Hyun,Kwon, Byoung-Mog,Kim, Se-Young,Baek, Nam-In 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.1
산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 3종의sterol 화합물을 분리, 정제하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ${\beta}-sitosterol$(1), $stigmast-4-en-6{\beta}-ol-3-one$(2), daucosterol(3)으로 동정하였다. 이 화합물들은 산딸나무에서 처음 분리되었다. The fruits of Cornus kousa Burg. were extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc fraction, three sterols were isolated through the repeated silica gel and ODS column chromatographies. According to the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as ${\beta}-sitosterol$ (1), $stigmast-4-en-6{\beta}-ol-3-one$ (2) and daucosterol (3). They were the first to be isolated from Cornus kousa Burg.
식용 식물자원으로부터 활성물질의 탐색-V. -상추(Lactuca sativa L.)의 ACAT 억제 Diterpenoid, Phytol-
장태오,방면호,송명종,홍윤희,김지영,정대균,배동근,권병목,김영국,이현선,김인호,백남인,Jang, Tae-O,Bang, Myun-Ho,Song, Myoung-Chong,Hong, Yoon-Hee,Kim, Ji-Young,Chung, Dae-Kyun,Pai, Tong-Kun,Kwon, Byung-Mok,Kim, Young-Kuk,Lee, Hyun-Sun,Kim 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.1
식용식물자원으로부터 활성물질의 탐색 XVI. -사자발쑥(Artemisia herba)의 전초로부터 sterol 화합물의 분리-
방면호,정해곤,송명종,유종수,정선아,이대영,김세영,정태숙,이경태,최명숙,백남인,Bang, Myun-Ho,Chung, Hae-Gon,Song, Myoung-Chong,Yoo, Jong-Su,Chung, Sun-A,Lee, Dae-Young,Kim, Se-Young,Jeong, Tae-Sook,Lee, Kyung-Tae,Choi, Myung-Sook,Baek, 한국응용생명화학회 2006 Applied Biological Chemistry (Appl Biol Chem) Vol.49 No.2
사자발쑥의 전초를 80% MeOH 용액으로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획과 n-BuOH 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography로 정제하여 4종의 sterol 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트림 데이터를 해석하여, ${\beta}-sitosterol$ (1), dehydroergosterol $5{\alpha},8{\alpha}-epidioxide$ (2), stigmasterol (3), daucosterol (4)으로 동정하였다. 이 화합물들은 사자발쑥에서 처음 분리되었다. Sajabalssuk (Artemisia herba) was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc and n-BuOH fractions, four sterols were isolated through the repeated silica gel and ODS column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the sterols were determined as ${\beta}-sitosterol$ (1), ergosterol peroxide (2), stigmasterol (3) and daucosterol (4). They were the first to be isolated from Sajabalssuk (Artemisia herba).