Low Molecular Weight Protamine/ Insulin Formulation with Potential to Attenuate Protamine-Masqueraded Insulin Allergy

Huining He,Victor C. Yang,Allan E. David,Jian Zhang,박윤신,Jianxin Wang,Yongzhuo Huang,Jingkang Wang 한국고분자학회 2011 Macromolecular Research Vol.19 No.12

Effect of the Ag-doping on the phase structural evolution, dielectric and ferroelectric properties of lead-free niobate ceramics

Huining Wang,Zixin Zhang,QuanSen Yang,Fangfang Wang,Kang Yan 대한금속·재료학회 2022 ELECTRONIC MATERIALS LETTERS Vol.18 No.2

Ionic doping is an important approach to modification of crystal structure and physical properties of the piezoelectric ceramics. In this work, the lead-free piezoelectric ceramic (1- x )K 0.18 Na 0.74 Li 0.08 NbO 3 - x AgNbO 3 (abbreviated as KNLN- x AN, 0 ≤ x ≤ 0.8) was prepared by conventional solid-state reaction method. The effect of the Ag doping on the phase structure, dielectric and ferroelectric properties of the KNLN-based ceramics was investigated by the XRD, DSC, dielectric and ferroelectric characterizations. It is found that all KNLN- x AN ceramics show single phase in perovskite structure because the ionic radii of dopant Ag close to the average ionic size at the A-site in KNLN. The KNLN- x AN exhibits the rhombohedral to orthorhombic phase transition at room temperature and the Curie temperature ( T C ) decreases to low temperature with increasing x value. A narrow tetragonal phase region and a wide orthorhombic phase region form for the compositions with x ≥ 0.4, indicating that the Ag doping stabilizes orthorhombic phase in wide temperature for the KNLN. A ferroelectric anomalies due to high electrical field induced phase transition is found in the sample with x = 0.1. When x = 0.4, high Curie temperature ( T C = 380 °C), large remnant polarization ( P r = 24.4 μC/cm 2 ) and high dielectric constant ( ε r = 10,000 at 1 kHz) at T C are achieved.

Experimental Investigation on Permeability Evolution of Limestone Caprock under Coupled THM Processes

Qiangxing Zhang,Jianfeng Liu,Huining Xu,Yin Zeng,Chunping Wang,Lu Wang 대한토목학회 2019 KSCE Journal of Civil Engineering Vol.23 No.12

To investigate the gas sealing capacity of limestone as cap rock for gas reservoir at different burial depths, triaxial compression coupled with permeability tests of limestone subjected to different confining pressures and temperatures were carried out using the improved MTS 815 test machine. During the tests, acoustic emission (AE) monitoring system was also employed to estimate the rock damage. Test results demonstrated a degradation of triaxial compression strength with the increase in temperature and decrease of confining pressure. An empirical equation for the peak stress was established considering confining pressure and temperature. Three different permeability evolution patterns of limestone samples can be proposed based on the experimental results under different coupled thermo-hydro-mechanical (THM) conditions. According to the spatial-temporal characteristics of AE signals, permeability evolution was highly related to the fracture growth and connection in limestone. Ultimately, the experimental results revealed that the permeability of limestone gradually decreased with the increasing confining pressure and temperature. In addition, an exponential relationship between initial permeability and confining pressure was established.

Design and behaviour of double skin composite beams with novel enhanced C-channels

Jia-Bao Yan,Huining Guan,Tao Wang 국제구조공학회 2020 Steel and Composite Structures, An International J Vol.37 No.5

This paper firstly developed a new type of Double Skin Composite (DSC) beams using novel enhanced C-channels (ECs). The shear behaviour of novel ECs was firstly studied through two push-out tests. Eleven full-scale DSC beams with ECs (DSCB-ECs) were tested under four-point loading to study their ultimate strength behaviours, and the studied parameters were thickness of steel faceplate, spacing of ECs, shear span, and strength of concrete core. Test results showed that all the DSCB-ECs failed in flexure-governed mode, which confirmed the effective bonding of ECs. The working mechanisms of DSCB-ECs with different parameters were reported, analysed and discussed. The load-deflection (or strain) behaviour of DSCB-ECs were also detailed reported. The effects of studied parameters on ultimate strength behaviour of DSCB-ECs have been discussed and analysed. Including the experimental studies, this paper also developed theoretical models to predict the initial stiffness, elastic stiffness, cracking, yielding, and ultimate loads of DSCB-ECs. Validations of predictions against 11 test results proved the reasonable estimations of the developed theoretical models on those stiffness and strength indexes. Finally, conclusions were given based on these tests and analysis.

Cardiac-derived stem cell engineered with constitutively active HIF-1a gene enhances blood perfusion of hindlimb ischemia

Xing Pei,Jiyoung Shin,김희정,Nana Wang,Chaewon Seo,Miyun Yoon,Xiongwen Chen,Jianqing Gao,Victor C. Yang,Huining He,Seungjin Lee 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.105 No.-

Stem cell-based therapeutic approach provides a possible treatment for critical limb ischemia (CLI) byinducing revascularization and regenerating ischemic tissue. However, the clinical benefit is modestdue to low cell survival and limited efficacy after transplantation. Cardiac-derived stem cells (CSCs) mightbe a novel cell source for CLI treatment owing to their superb endothelial differentiation potential andangiogenic paracrine functions. In this study, the angiogenic ability of CSCs was maximized by geneticengineering with constitutively active form of hypoxia-inducible factor-1a (CA-HIF-1a), resistant tooxygen-dependent degradation. CSCs transfected with CA-HIF-1a (CA-HIF-CSCs) promoted supplementaryexpression of proangiogenic factors including VEGF, bFGF, Ang-1 and PDGF-B, along with enhancedangiogenic function including migratory effect, tube formation and endothelial differentiation potential. In the mouse CLI model, CA-HIF-CSCs transplanted into the ischemic region using fibrin gel as cell deliveryvehicle, improved blood perfusion and limb functional recovery with minimal incidence of foot necrosisand limb loss by promoting new vessel formation. Histological evidence further confirmed that CAHIF-CSC/gel treatment markedly alleviated muscle degeneration and fibrosis. CSCs genetically engineeredwith constitutively active HIF-1a provide a novel therapeutic modality in CLI combining stem cell andgene therapy.

MMP-2-responsive fluorescent nanoprobes for enhanced selectivity of tumor cell uptake and imaging

Sun, Lu,Xie, Shuping,Ji, Xiuru,Zhang, Jingming,Wang, Dongmei,Lee, Seung Jin,Lee, Hyukjin,He, Huining,Yang, Victor C. The Royal Society of Chemistry 2018 Biomaterials Science Vol.6 No.10

<P>It is difficult to develop highly selective substrate-based fluorescent nanoprobes for specific matrix metalloproteinases (MMPs) due to overlapping substrate specificities among the family of MMP enzymes. To resolve this issue, we have developed novel fluorescent nanoprobes that are highly selective for soluble MMP-2. Herein, MMP-2-responsive nanoprobes were prepared by immobilizing fluorescent fusion proteins on nickel ferrite nanoparticles <I>via</I> the His-tag nickel chelation mechanism. The fusion protein consisted of a fluorescent mCherry protein with a cell penetrating peptide (CPP) moiety. An MMP-2 cleavage site was also introduced within the fusion protein, which was directly linked to the nickel ferrite nanoparticles. The selectivity of nanoprobes was modulated by hiding the cleavage site of MMP-2 substrates deeply inside the system, which could result in strong steric hindrance between the nanoprobes and MMPs, especially for membrane-tethered MMPs such as MMP-14. A cell-based assay demonstrated that the nanoprobes could only be activated by tumor cells secreting soluble MMP-2, but not membrane-tethered MMP-14. To further evaluate the contribution of the steric hindrance effect on the nanoprobes, a truncated recombinant MMP-14 was employed to confer their cleavage activity as compared to native membrane-tethered MMP-14. Furthermore, a designed probe with a diminished steric hindrance effect was proved to be activated by membrane-tethered type MMP-14. The results indicated that the design of fluorescent nanoprobes employing the steric hindrance effect can greatly enhance the selectivity of MMP-responsive nanoprobes realizing the specific detection of soluble MMP-2 in a tumor microenvironment. We believe that highly selective MMP-2-responsive fluorescent nanoprobes have broad impacts on biomedical applications including molecular imaging and labeling for tumor detection.</P>

Cellular uptake mechanism and comparative in vitro cytotoxicity studies of monomeric LMWP-siRNA conjugate

Junxiao Ye,Xing Pei,Hui Cui,Zhili Yu,이혁진,Jian-Xin Wang,Xu Wang,Lu Sun,Huining He,Victor C. Yang 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.63 No.-

The covalent attachment of CPPs to siRNA molecules offers great potential for CPP-mediated siRNA delivery. We recently reported a concise and high-yield synthesis strategy of the cell-permeable, cytosol-dissociable LMWP-siRNA covalent conjugate. Herein, cell uptake mechanism and cellular toxicity studies of this conjugate were performed to evaluate the potential of LMWP-siRNA conjugate for clinical translation. Cellular uptake mechanism study indicated that the conjugate could be taken up by cells via multiple pathways, including direct penetration of the plasma membrane and clathrin- and caveolae-independent endocytosis. In vitro cytotoxicity study revealed that the conjugation promoted internalization in a low-toxic fashion.

Aerobic Denitrification: A Review of Important Advances of the Last 30 Years

Bin Ji,Kai Yang,Lei Zhu,Yu Jiang,Hongyu Wang,Jun Zhou,Huining Zhang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

Understanding aerobic denitrification has become an important focus of environmental microbiology. Aerobic denitrification can be performed by various genera of microorganisms and describes the use of nitrate (NO3 −) as oxidizing agents under an aerobic atmosphere. Isolation of aerobic denitrifiers, enzymes involved in aerobic denitrifiers, phylogenetic distribution of aerobic denitrifiers, factors affecting the performance of aerobic denitrifiers, attempts of applications and possible future trends are depicted. The periplasmic nitrate reductase is vital for aerobic denitrifiers and NapA gene may be the proof of aerobic denitrification. Phylogenetic analysis revealed that aerobic denitrifiers mainly belong to α-, β- and γ-Proteobacteria. Aerobic denitrifiers tend to work efficiently at 25 ~ 37°C and pH 7 ~ 8, when dissolved oxygen concentration is 3 ~ 5 mg/L and C/N load ratio is 5 ~ 10. In addition, recent progresses and applications on aerobic denitrifiers are described, including single aerobic reactors, sequencing batch reactor and biofilm reactors. The review attempts to shed light on the fundamental understanding in aerobic denitrification.

Cellular uptake mechanism and comparative <i>in vitro</i> cytotoxicity studies of monomeric LMWP-siRNA conjugate

Ye, Junxiao,Pei, Xing,Cui, Hui,Yu, Zhili,Lee, Hyukjin,Wang, Jianxin,Wang, Xu,Sun, Lu,He, Huining,Yang, Victor C. THE KOREAN SOCIETY OF INDUSTRIAL AND ENGINEERING 2018 JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY -S Vol.63 No.-

<P><B>Abstract</B></P> <P>The covalent attachment of CPPs to siRNA molecules offers great potential for CPP-mediated siRNA delivery. We recently reported a concise and high-yield synthesis strategy of the cell-permeable, cytosol-dissociable LMWP-siRNA covalent conjugate. Herein, cell uptake mechanism and cellular toxicity studies of this conjugate were performed to evaluate the potential of LMWP-siRNA conjugate for clinical translation. Cellular uptake mechanism study indicated that the conjugate could be taken up by cells via multiple pathways, including direct penetration of the plasma membrane and clathrin- and caveolae-independent endocytosis. <I>In vitro</I> cytotoxicity study revealed that the conjugation promoted internalization in a low-toxic fashion.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>