MUC16 facilitates cervical cancer progression via JAK2/STAT3 phosphorylation‑mediated cyclooxygenase‑2 expression

Hui Shen,Meng Guo,Lu Wang,Xinyue Cui 한국유전학회 2020 Genes & Genomics Vol.42 No.2

Objectives MUC16 (mucin 16, also known as CA-125, cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) has been predicted as tumor biomarker for therapy. We determined to investigate effects and regulatory mechanism of MUC16 on cervical tumorigenesis. Methods Expression levels of MUC16 in cervical cancer cell lines was analyzed via qRT-PCR (quantitative real-time polymerase chain reaction). Knockdown of MUC16 was conducted via shRNA (Short hairpin RNA) transfection. MTT and colony formation assays were used to investigate effect of MUC16 on cell proliferation. Wound healing assay was utilized to detect migration and transwell assay to detect invasion. The underlying mechanism was demonstrated via western blot analysis. Results MUC16 was elevated in cervical cancer cell lines. MUC16 knockdown inhibited cell proliferation, invasion and migration. Gain- and loss-of functional assays revealed that over-expression of MUC16 activated Janus Kinase 2 (JAK2)/ signal transducer and activator of transcription 3 (STAT3) via phosphorylation, thus facilitating cyclooxygenase-2 (COX-2) expression, while knockdown of MUC16 demonstrated the reverse effect on JAK2/STAT3 activation and COX-2 expression. Moreover, inhibition of JAK2/STAT3 attenuated the regulation of MUC16 on COX-2. Conclusions MUC16 enhanced proliferation and invasion of cervical cancer cells via JAK2/STAT3 phosphorylation-mediated cyclooxygenase-2 expression, suggesting the potential therapeutic target ability of MUC16 to treat cervical cancer.

적응적 방법에 의한 하이퍼큐브 네트워크 결함 진단 알고리즘

최혜연,김선신,강성수,최길호,이충세 충북대학교 컴퓨터정보통신 연구소 2003 컴퓨터정보통신연구 Vol.11 No.2

시스템 레벨의 대부분의 진단 알고리즘은 PMC 모델을 바탕으로 결함의 개수가 t개를 초과하지 않는다는 t-진단가능 시스템의 특성을 이용하여 결함을 진단한다. 그러나, 다중처리 시스템 상에서 큰 규모의 결함 집합을 고려할 때에 여러 가지 제약이 따른다. 또한 병렬처리 시스템의 규모가 커짐에 따라 시스템 내에서 발생되는 결함의 빈도가 높아지게 된다. 진단 알고리즘에서 가정하는 결함의 개수 t는 병렬처리 시스템 안에 있는 노드의 수에 비해 상당히 작은 개수이며, 결함의 개수가 t개를 초과할 경우의 연구는 거의 이루어지지 않았다. Somani와 Peleg은 k개의 부정확한 진단을 용인함으로써 결함의 개수가 t개를 초과할 경우에도 시스템을 진단하는 t/k-dignosable 시스템을 제안하였다. 이 논문에서는 Somani 등이 제안한 것처럼 k=1개의 부정확한 진단을 용인하는 경우에 하이퍼큐브를 진단하는 알고리즘을 제안한다. 이 논문에서 제안한 알고리즘은 더 많은 결함을 진단하면서도 기존의 알고리즘보다 효율이 거의 떨어지지 않는다는 사실을 분석을 통하여 확인할 수 있었다. System level diagnosis algorithms are based on the PMC model and use the properties of t-diagnosable system where the maximum number of the faults does not exceed t. Diagnostic algorithms have limit when dealing with large fault sets in multiprocessor systems. Somani and Peleg proposed t/k-diagnosable system to system diagnose by allowing upper bounded few number of units to be diagnosed incorrectly, which can diagnosis more faults than t-diagnosable system. In this paper, we propose hypercube diagnosis algorithm using t/k-diagnosable system. When the number of faults exceeds t, we allow a fault(k=1) to be diagnosed incorrectly, Based on this idea, we find that the performance of the proposed algorithm is nearly as efficient as any previously known strategies and detect more faults.

Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

3차원 웨이블릿 기반 영상 압축 기술에 관한 연구

CUI HUI ZI,문주희(Joo-hee Moon) 대한전자공학회 2007 대한전자공학회 학술대회 Vol.2007 No.7

In previous work, each codeblock is coded using a context which is adaptively selected from three kinds of scanning directions after 3D DWT. But some subbands still have correlations among the coefficients in horizontal, vertical or temporal direction. In this paper, we propose a new 3D DWT-based video compression technique in which the difference of coefficients is calculated in one of three directions for every codeblocks and coded by a context adaptively selected for each codeblock. Experimental results show that the proposed compression technique outperforms measurably and visually compared to the conventional DWT-based techniques.

Electroreduction of H2O2 by Co3O4 and NiCo2O4 nanowires and beta-Ni(OH)2 nanoplates grown on Ni foam.

Hui, K N,Yin, Cui-Lei,Hui, K S,Lee, J Y,Cao, Dianxue,Wang, Guiling American Scientific Publishers 2012 Journal of nanoscience and nanotechnology Vol.12 No.4

<P>Nanowires (Co3O4 and NiCo2O4) and nanoplates (beta-Ni(OH)2) grown on Ni foam are successfully prepared by a template-free method and used as cathode electrodes for the electroreduction of H2O2, in an alkaline medium. Catalytic performance is investigated via cyclic voltammetry and chronoamperometry. The Co3O4 and NiCo2O4 nanowire electrodes exhibit much better catalytic activity, stability, and mass transfer properties for H2O2 electroreduction than pressed Co3O4 and NiCo2O4 nanoparticle/carbon/PTFE electrodes. A current density of 101.8 mA cm(-2) and 122.7 mA cm(-2) are respectively achieved on Co3O4 and NiCo2O4 nanowire electrodes at -0.4 V in 0.4 mol/L H2O2, and 3.0 mol/L NaOH solution at room temperature.</P>

The Relative Influence of Diet and Physical Activity on Obesity in China

Cui Zhao-Hui,Li Yan-Ping,Di Yu-Feng,Ba Lei,Hu Xiaoqi,Ma Guan-Sheng The Korean Society of Community Nutrition 2004 Journal of community nutrition Vol.6 No.3

The purpose of this study is to investigate the relative influence of diet and physical activity on obesity. The subjects were 155 adults aged 35-52 years from 24 neighborhood committees in 4 urban districts of Beijing (male : 78, female : 77). They were divided into normal weight, overweight and obese groups according to their BMI. The general information of the subjects was collected by interview-administered questionnaire. Dietary intake was obtained by three-day(two weekdays and one weekend day) food weighted method, physical activity was assessed by a validated combination of data obtained from activity monitors, bicycling information and activity records. There were no significant differences of age, gender, height, educational, family economic level, smoking and drinking between different groups. The proportion of flour intake was higher in obese group compared to normal weight and overweight groups, and that of vegetables is lower in obese group. The physical activity (PAL) was not significantly different between two groups of the normal, overweight and obese groups. After the adjustment for confounding factors using logistic regression model, we found that the proportion of flour intake was positively associated with obesity, while the proportion of vegetable intake was inversely associated with obesity. It is concluded that dietary patterns were associated with obesity and diets composed of more vegetables and less staple combined with physical activities could contribute to obesity prevention.

Inorganic Glue Enabling High Performance of Silicon Particles as Lithium Ion Battery Anode

Cui, Li-Feng,Hu, Liangbing,Wu, Hui,Choi, Jang Wook,Cui, Yi The Electrochemical Society 2011 Journal of the Electrochemical Society Vol.158 No.5

Protective action of 9-hydroxypinoresinol against oxidative damage in brain of mice challenged with kainic acid

Cui, Hui Song,Sok, Dai-Eun,Min, Byung Sun,Kim, Mee Ree Pharmaceutical Society of Great Britain 2007 Journal of pharmacy and pharmacology Vol.59 No.4

Wound Healing Potential of Low Temperature Plasma in Human Primary Epidermal Keratinocytes

Hui Song Cui,Yoon Soo Cho,So Young Joo,Chin Hee Mun,Cheong Hoon Seo,June-Bum Kim 한국조직공학과 재생의학회 2019 조직공학과 재생의학 Vol.16 No.6

BACKGROUND: Low temperature plasma (LTP) was recently shown to be potentially useful for biomedical applications such as bleeding cessation, cancer treatment, and wound healing, among others. Keratinocytes are a major cell type that migrates directionally into the wound bed, and their proliferation leads to complete wound closure during the cutaneous repair/regeneration process. However, the beneficial effects of LTP on human keratinocytes have not been well studied. Therefore, we investigated migration, growth factor production, and cytokine secretion in primary human keratinocytes after LTP treatment. METHODS: Primary cultured keratinocytes were obtained from human skin biopsies. Cell viability was measured with the EZ-Cytox cell viability assay, cell migration was evaluated by an in vitro wound healing assay, gene expression was analyzed by quantitative real-time polymerase chain reaction, and protein expression was measured by enzyme-linked immunosorbent assays and western blotting after LTP treatment. RESULTS: Cell migration, the secretion of several cytokines, and gene and protein levels of angiogenic growth factors increased in LTP-treated human keratinocytes without associated cell toxicity. LTP treatment also significantly induced the expression of hypoxia inducible factor-1a (HIF-1a), an upstream regulator of angiogenesis. Further, the inhibition of HIF- 1a expression blocked the production of angiogenic growth factors induced by LTP in human keratinocytes. CONCLUSION: Our results suggest that LTP treatment is an effective approach to modulate wound healing-related molecules in epidermal keratinocytes and might promote angiogenesis, leading to improved wound healing.

Performance Improvement by Controlling Se/metal Ratio and Na₂S Post Deposition Treatment in Cu(In,Ga)₃Se<SUB>5</SUB> Thin-Film Solar cell

Hui-Ling Cui,Seung Tae Kim,R.B.V. Chalapathy,Ji Hye Kim,Byung Tae Ahn 한국태양광발전학회 2019 Current Photovoltaic Research Vol.7 No.4

Cu(In,Ga)₃Se5 (β-CIGS) has a band gap of 1.35 eV, which is an optimum value for high solar-energy conversion efficiency. The effects of Cu and Ga content on the cell performance were investigated previously. However, the effect of Se content on the cell performance is not well understood yet. In this work, β-CIGS films were fabricated by three-stage co-evaporation of elemental sources with various Se fluxes at the third stage instead of at all stages. The average composition of five samples was Cu1.05(In0.59,Ga0.41)₃Sey, where the stoichiometric y value is 5.03 and the stoichiometric Se/metal (Se/M) ratio is 1.24. We varied the Se/metal ratio in a range from 1.18 to 1.28. We found that the best efficiency was achieved when the Se/M ratio was 1.24, which is exactly the stoichiometric value where the CIGS grains on the CIGS surface were tightly connected and faceted. With the optimum Se/M ratio, we were able to enhance the cell efficiency of a β-CIGS solar cell from 9.6% to 12.0% by employing a Na2S post deposition treatment. Our results indicate that Na₂S post deposition treatment is very effective to enhance the cell efficiency to a level on par with that in α-CIGS cell.