Effects of Lactobacillus curvatus and Leuconostoc mesenteroides on Suan Cai Fermentation in Northeast China^s

( Hongyan Yang ),( Hao Wu ),( Lijuan Gao ),( Hongbai Jia ),( Yuan Zhang ),( Zongjun Cui ),( Yuhua Li ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.12

To investigate the effects of Lactobacillus curvatus and Leuconostoc mesenteroides on suan cai (pickled Chinese cabbage) fermentation, L. curvatus and/or Ln. mesenteroides were inoculated into suan cai. Physicochemical indexes were measured, and the microbial dynamics during the fermentation were analyzed by Illumina MiSeq sequencing and quantitative polymerase chain reaction (qPCR). The results showed that inoculation with lactic acid bacteria (LAB) lowered the pH of the fermentation system more rapidly. The decrease in water-soluble carbohydrates in the inoculated treatments occurred more rapidly than in the control. The LAB counts in the control were lower than in other inoculated treatments during the first 12 days of fermentation. According to the Illumina MiSeq sequencing analyses, Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Cyanobacteria, Fusobacteria, and Verrucomicrobia were present in the fermentations, along with other unclassified bacteria. Generally, Firmicutes was predominant during the fermentation in all treatments. At the genus level, 16 genera were detected. The relative abundance of Lactobacillus in all inoculated treatments was higher than in the control. The relative abundance of Lactobacillus in the treatments containing L. curvatus was higher than in the Ln. mesenteroides-only treatment. The relative abundance of Leuconostoc in the Ln. mesenteroides-containing treatments increased continuously throughout the fermentation. Leuconostoc was highest in the Ln. mesenteroides-only treatment. According to the qPCR results, L. curvatus and/or Ln. mesenteroides inoculations could effectively inhabit the fermentation system. L. curvatus dominated the fermentation in the inoculated treatments.

Parameters Estimation of the Mathematical Model of Solid Oxide Fuel Cell Stacks based on a Fractional‑order Water Strider Algorithm

Wu Minrong,Li Shanshan,Chen Hongyan,Duan Wenqi,Shafiee Mohammadreza 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.1

In recent decades, in the use of science and technology, extensive research has been conducted on an academic and industrial scale to introduce new sources of energy that are fully constructed to meet the needs of today’s human beings. One of these new sources that use your special energy is a fuel cell. A fuel cell is a clean source that converts the chemical energy in a fuel directly into electrical energy by performing two separate electrochemical reactions separately. Providing a proper model for the Solid Oxide Fuel Cell (SOFC) as one of the useful fuel cells is critical for reducing the costs of the design. However, the complexity of this problem made it a challenging task owing to the complex and highly nonlinear nature. Particularly, for SOFCs, the model should be optimized under diff erent temperature and pressure operating conditions. The present study proposes a newly developed version of the Water Strider Algorithm, called Fractional-order Water Strider Algorithm (FOWSA) for optimal identifi cation of the SOFC parameters. Using the proposed FOWSA is introduced to improve the original WSA to provide better convergence and global optimization results. The effi ciency of the suggested FOWSA has been fi rstly validated and then, the designed method is performed to a practical case study and the sensitivity analysis in terms of temperature and pressure. The fi nal results indicate that the suggested method shows outstanding effi ciency toward the compared methods.

Research on facility layout optimization algorithm of deep-water semi-submersible drilling platform

Hongyan Wang,Wensheng Xiao,Lei Wu,Keke Wei,Congcong Xu,Chao Hou,Junguo Cui,Jie Li 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.2

We aimed to create a facility layout design of semi-submersible drilling platform (DSDP) with performance constraints. The Boltzmann survival mechanism of simulated annealing algorithm was introduced into the replacement strategy of genetic algorithm to form an improved genetic algorithm called genetic and simulated annealing algorithm (GASA). The new algorithm alleviates the “combination explosion” and premature convergence of the traditional genetic algorithm. The layout problem of DSDP is efficiently solved by using this new algorithm. When the number of layout objects increases, GASA’s performance is better than that of the genetic algorithm.

Overexpression of the Polygonum cuspidatum PcDREB2A Gene Encoding a DRE-Binding Transcription Factor Enhances the Drought Tolerance of Transgenic Arabidopsis thaliana

Hu Hongyan,Wang Xiaowei,Wu Zhijun,Chen Mo,Chai Tuanyao,Wang Hong 한국식물학회 2022 Journal of Plant Biology Vol.65 No.6

Plants have evolved complex signaling networks that enable them to adapt to adverse environmental conditions. The dehydration-responsive element-binding (DREB) transcription factors are important for plant responses to abiotic stresses. In this study, a new member of the AP2/ERF transcription factor gene family, PcDREB2A, was cloned and characterized from Polygonum cuspidatum, a traditional Chinese medicinal herb. PcDREB2A, which includes a typical AP2 domain, was clustered in the A-2 subgroup of the DREB subfamily. At the seedling stage, PcDREB2A expression was induced by cold, salt, and drought stresses. A yeast one-hybrid assay and an analysis of transiently transformed tobacco revealed that PcDREB2A can specifically bind to the DRE motif and transactivate reporter gene expression. Following 200 and 250 mM mannitol treatments, the PcDREB2A-overexpressing Arabidopsis thaliana lines had longer roots and a significantly higher fresh weight than the wild-type plants. Furthermore, under drought stress conditions, the PcDREB2A-overexpressing A. thaliana plants accumulated less malondialdehyde than the control plants. These results indicate that PcDREB2A encodes a novel DREB transcription factor in P. cuspidatum. Furthermore, the data generated in this study may be useful for researchers and breeders interested in genetically engineering plants to increase drought tolerance without inhibiting growth.

Secondary Voltage Control for Reactive Power Sharing in an Islanded Microgrid

Qian Guo,Hongyan Wu,Liaoyuan Lin,Zhihong Bai,Hao Ma 전력전자학회 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.1

Owing to mismatched feeder impedances in an islanded microgrid, the conventional droop control method typically results in errors in reactive power sharing among distributed generation (DG) units. In this study, an improved droop control strategy based on secondary voltage control is proposed to enhance the reactive power sharing accuracy in an islanded microgrid. In a DG local controller, an integral term is introduced into the voltage droop function, in which the voltage compensation signal from the secondary voltage control is utilized as the common reactive power reference for each DG unit. Therefore, accurate reactive power sharing can be realized without any power information exchange among DG units or between DG units and the central controller. Meanwhile, the voltage deviation in the microgrid common bus is removed. Communication in the proposed strategy is simple to implement because the information of the voltage compensation signal is broadcasted from the central controller to each DG unit. The reactive power sharing accuracy is also not sensitive to time-delay mismatch in the communication channels. Simulation and experimental results are provided to validate the effectiveness of the proposed method.

Secondary Voltage Control for Reactive Power Sharing in an Islanded Microgrid

Guo, Qian,Wu, Hongyan,Lin, Liaoyuan,Bai, Zhihong,Ma, Hao The Korean Institute of Power Electronics 2016 JOURNAL OF POWER ELECTRONICS Vol.16 No.1

Owing to mismatched feeder impedances in an islanded microgrid, the conventional droop control method typically results in errors in reactive power sharing among distributed generation (DG) units. In this study, an improved droop control strategy based on secondary voltage control is proposed to enhance the reactive power sharing accuracy in an islanded microgrid. In a DG local controller, an integral term is introduced into the voltage droop function, in which the voltage compensation signal from the secondary voltage control is utilized as the common reactive power reference for each DG unit. Therefore, accurate reactive power sharing can be realized without any power information exchange among DG units or between DG units and the central controller. Meanwhile, the voltage deviation in the microgrid common bus is removed. Communication in the proposed strategy is simple to implement because the information of the voltage compensation signal is broadcasted from the central controller to each DG unit. The reactive power sharing accuracy is also not sensitive to time-delay mismatch in the communication channels. Simulation and experimental results are provided to validate the effectiveness of the proposed method.

Gastrin stimulates pancreatic cancer cell directional migration by activating the Gα12/13–RhoA–ROCK signaling pathway

Ganggang Mu,Qianshan Ding,Hongyan Li,Li Zhang,Lingli Zhang,Ke He,Lu Wu,Yunchao Deng,Dongmei Yang,Lianlian Wu,Ming Xu,Jie Zhou,Honggang Yu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The mechanism by which gastrin promotes pancreatic cancer cell metastasis is unclear. The process of directing polarized cancer cells toward the extracellular matrix is principally required for invasion and distant metastasis; however, whether gastrin can induce this process and its underlying mechanism remain to be elucidated. In this study, we found that gastrin-induced phosphorylation of paxillin at tyrosine 31/118 and RhoA activation as well as promoted the metastasis of PANC-1 cancer cells. Depletion of Gα12 and Gα13 inhibited the phosphorylation of paxillin and downstream activation of GTP-RhoA, blocked the formation and aggregation of focal adhesions and facilitated polarization of actin filaments induced by gastrin. Suppression of RhoA and ROCK also exhibited identical results. Selective inhibition of the CCKBR–Gα12/13–RhoA–ROCK signaling pathway blocked the reoriented localization of the Golgi apparatus at the leading edge of migrated cancer cells. YM022 and Y-27632 significantly suppressed hepatic metastasis of orthotic pancreatic tumors induced by gastrin in vivo. Collectively, we demonstrate that gastrin promotes Golgi reorientation and directional polarization of pancreatic cancer cells by activation of paxillin via the CCKBR–Gα12/13–RhoA–ROCK signal pathway.

Burn-resistant Coatings Lubricated on the Surface of Titanium Alloys

Pingze Zhang,Jun Huang,Hongyan Wu,Qiang Miao,Zhengjun Yao,Zhong Xu,Zhiyong He 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.-

Conventional titanium alloy may be ignited and burnt in conditions of high temperature, high air pressure, and high gas flow velocity. In the present study, burn-resistant coatings have been lubricated by using double glow plasma surface alloying technology. Alloying elements Cr, Mo, and Cu were induced into Ti6Al4V and Ti6.5Al0.3Mo1.5Zr0.25Si substrates through double glow discharged plasma processing, and Ti-Cr, Ti-Mo, and Ti-Cu burn-resistant alloy layers were formed on the surface of the Ti6Al4V and Ti6.5Al0.3Mo1.5Zr0.25Si alloys. The depth of the surface burn-resistant alloy layers could exceed 200 μm and the alloying-element component could reach 90%. Burn-resistant performance experimental results show that if the Cr component(wt%) is over 14%, the Cu component is over 12%, and the Mo component is over 10% in the alloying layers, ignition and burn of titanium alloy are effectively avoided. Conventional titanium alloy may be ignited and burnt in conditions of high temperature, high air pressure, and high gas flow velocity. In the present study, burn-resistant coatings have been lubricated by using double glow plasma surface alloying technology. Alloying elements Cr, Mo, and Cu were induced into Ti6Al4V and Ti6.5Al0.3Mo1.5Zr0.25Si substrates through double glow discharged plasma processing, and Ti-Cr, Ti-Mo, and Ti-Cu burn-resistant alloy layers were formed on the surface of the Ti6Al4V and Ti6.5Al0.3Mo1.5Zr0.25Si alloys. The depth of the surface burn-resistant alloy layers could exceed 200 μm and the alloying-element component could reach 90%. Burn-resistant performance experimental results show that if the Cr component(wt%) is over 14%, the Cu component is over 12%, and the Mo component is over 10% in the alloying layers, ignition and burn of titanium alloy are effectively avoided.

Increased brain uptake of venlafaxine loaded solid lipid nanoparticles by overcoming the efflux function and expression of P-gp

Yan Zhou,Xin’an Wu,Guo-Qiang Zhang,Zhi Rao,Yang Yang,Qian Zhou,Hongyan Qin,Yuhui Wei 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.7

Venlafaxine (VLX) could be pumped out of the brain by P-glycoprotein (P-gp). Moreover, the expression of P-gp distributed in blood–brain barrier could be significantly induced by VLX. Thus, P-gp could be considered as the nature barrier for delivering of VLX to the brain. The aim of this study was to investigate whether the efflux function and increased expression of P-gp could be reversed by utilizing solid lipid nanoparticles (SLN). VLX solid lipid nanoparticles (VLX - SLN) were prepared and evaluated. Pharmacokinetics and brain distribution of VLX in different formulations were conducted after oral or intravenous administration. P-gp efflux function to VLX was evaluated by the brain uptake amount of VLX, while P-gp expression was investigated by Western blotting. Results indicated that the entrapment, mean size and zata potential of VLX - SLN was 74.9 ± 3.0 %, 186.3 ± 69.26 nm and -22.8 ± 7.78 mv, respectively. After vein injection of VLX formulations, the brain uptake amount of VLX from VLX - SLN was significantly higher than that of VLX solution, VLX solution with empty SLN (VLX? empty SLN) and VLX solution with Verapamil (VLX ? Ver), respectively. Furthermore, the protein mass of P-gp in VLX - SLN treated group was the lowest among all the investigated groups. These results indicated that SLN could overcome P-gp and achieve brain target by intravenous administration.

Prognostic Perspectives of STING and PD-L1 Expression and Correlation with the Prognosis of Epstein-Barr Virus-Associated Gastric Cancers

( Qi Sun ),( Yao Fu ),( Xiaobing Chen ),( Lin Li ),( Hongyan Wu ),( Yixuan Liu ),( Haojun Xu ),( Guoren Zhou ),( Xiangshan Fan ),( Hongping Xia ) 대한소화기기능성질환·운동학회 2022 Gut and Liver Vol.16 No.6

Background/Aims: Epstein-Barr virus-associated gastric cancers (EBVaGCs) have unique molecular and clinicopathological characteristics. The cyclic GMP-AMP synthase-stimulator of interferon genes (STING) pathway is recently recognized as the critical innate immunity against pathogens and tumors. STING is also a master regulator in the cancer-immunity cycle and targeting STING could synergize with existing immune-checkpoint therapies. However, the role of STING in GC, especially in EBVaGC, and its correlation with programmed death-ligand 1 (PD-L1) remain largely unclear. Methods: We collected 78 cases of EBVaGCs and 210 cases of EBV-negative GC (EBVnGC) from a total of 1,443 cases of GC analyzed by EBV-encoded small RNA in situ hybridization. We investigated STING and PD-L1 expression and their concomitant prognostic value in EBVaGCs and EBVnGCs using tissue microarray and immunohistochemistry. The effects of STING and PD-L1 expression on the overall survival of patients with EBVaGC or EBVnGC were assessed by univariate and multivariate analysis. Results: We found that both STING and PD-L1 exhibited significantly higher expression in the EBVaGCs than that in the EBVnGCs. The expression of STING was positively correlated with that of PD-L1 in EBVaGCs. Simultaneous negative expression of STING and PD-L1, and positive expression of STING were independent prognostic risk factors for EBVaGC and EBVnGC, respectively. Conclusions: This is the first prognostic retrospective study of STING and PD-L1 expression and the prognosis among EBVaGC and EBVnGC. The expression and prognostic value of STING and PD-L1 are different in the two types of GCs. STING and PD-L1 are promising prognostic biomarkers and therapeutic targets for EBVaGC and EBVnGC. (Gut Liver 2022;16:875-891)