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Yin, Zhihong,Zhao, Xin,Wang, Zhun,Li, Zhen,Bai, Rui,Yang, Shanshan,Zhao, Min,Pang, Quanhai Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.10
Guanine nucleotide-binding protein subunit alpha-s ($Gn{\alpha}s$) is a small subunit of the G protein-couple signaling pathway, which is involved in the formation of coat color. The expression level and distribution of $Gn{\alpha}s$ were detected by quantitative real-time-polymerase chain reaction (qPCR), western blot, and immunohistochemistry to investigate the underlying mechanisms of coat color in white and black skin tissues of mice. qPCR and western blot results suggested that $Gn{\alpha}s$ was expressed at significantly higher levels in black mice compared with that of white mice, and transcripts and protein possessed the same expression in both colors. Immunohistochemistry demonstrated $Gn{\alpha}s$ staining in the root sheath and dermal papilla in hair follicle of mice skins. The results indicated that the $Gn{\alpha}s$ gene was expressed in both white and black skin tissues, and the expression level of $Gn{\alpha}s$ in the two types of color was different. Therefore, $Gn{\alpha}s$ may be involved in the coat color formation in mice.
Hayat Faisal,Bai Yang,Iqbal Shahid,Ma Chengdong,Ali Muhammad Moaaz,Shahid Muhammad Adnan,Hasan Mahmood Ul,Mosa Walid F. A.,Khan Ummara,Xiao Huang,Gao Zhihong 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.5
Indole-3-acetic acid (IAA) is an important plant hormone required for various plant growth and developmental activities. YUCCA proteins are the enzymes for IAA synthesis that catalyze the rate-limiting step. However, the YUCCA gene family in Japanese apricot has not been thoroughly characterized. Here, we investigated the eff ect of rootstocks on scion growth of Japanease apricot and genes involved in auxin biosynthesis ( YUCCA ). Growth characteristics revealed that the height of ‘Longyan’ [ Prunus mume Sieb. et Zucc] plants with P. mume rootstock were signifi cantly shorter than ‘Longyan’ plants with P. persica rootstock. This study isolated 13 PmYUCCA genes from the Japanese apricot genome. Bioinformatics and expression studies were undertaken to investigate the functions and characteristics of YUCCA genes. The physicochemical properties, gene structure, conserved domains, conserved motif, and phylogenetic relationships of YUCCA genes were determined using bioinformatics analysis. Likewise, we analyzed the expression of PmYUCCA genes in Japanese apricot’s leaf and stem-bark (scion). The expression levels of PmYUCCA3 and PmYUCCA5 genes were noticeably lower in the leaves of ‘Longyan’/ P. mume graft combination than that of the ‘Longyan’/ P. persica graft combinations, suggesting their distinct roles in regulating growth vigor. This research explores the genome-wide identifi cation, characterization, and possible relationship between growth vigor and expression profi le analysis of the YUCCA gene family in Japanese apricot.
Lin, Liaoyuan,Guo, Qian,Bai, Zhihong,Ma, Hao The Korean Institute of Power Electronics 2017 JOURNAL OF POWER ELECTRONICS Vol.17 No.1
There exists a strong coupling between real and reactive power owing to the complex impedances in droop based islanded microgrids (MGs). The existing virtual impedance methods consider improvements of the impedance matching for sharing of the voltage controlled power (VCP) (reactive power for Q-V droop, and real power for P-V droop), which yields a 1-DOF (degree of freedom) tunable virtual impedance. However, a weak impedance matching for sharing of the frequency controlled power (FCP) (real power for $P-{\omega}$ droop, and reactive power for $Q-{\omega}$ droop) may result in FCP overshoots and even oscillations during load transients. This in turn results in VCP oscillations due to the strong coupling. In this paper, a 2-DOF tunable adaptive virtual impedance method considering impedance matching for both real and reactive power (IM-PQ) is proposed to improve the power sharing performance of MGs. The dynamic response is promoted by suppressing the coupled power oscillations and power overshoots while realizing accurate power sharing. In addition, the proposed power sharing controller has a better parametric adaptability. The stability and dynamic performances are analyzed with a small-signal state-space model. Simulation and experimental results are presented to investigate the validity of the proposed scheme.
Minghan. Dong,Hao. Ma,Zhihong. Bai 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5
Dynamic currents and voltages in power electronic converters often exceed rating when changing load. In this way, this paper researches on optimizing transient performance of LCL-type gird-connected inverter systems. Resistor connected in series with capacitor as passive damping (RCSCPD), which is widely used to damp resonance of LCL filter, needs to choose an appropriate resistor to acquire accurate and fast transient performance. To meet this requirement, an approach is proposed for analyzing and improving transient performance. It shows that passive resistor could damp resonance and stable system. Limitation of RCSCPD, which could not optimize dominant transient response, is exhibited as well. Hence, mix damping (MD) is proposed to achieve the goal. It could eliminate overshoot and decrease settling time efficiently when comparing with RCSCPD. MD could configure the poles effectively without adding precision sensors because it uses grid current, which used in control loop, to realize active damping resistor. Experimental results based on single-phase LCL-type gird-connected inverter prototype are given to show optimizing effects of transient performance.
Zhongshan Wang,Haiyan Qin,Zhihong Feng,Guofeng Wu,Shizhu Bai,Yan Dong,Yimin Zhao 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3
Bacterial invasion and epithelial downgrowth with pocket formation are still severe clinical challenges for transcutaneous implants, and both have a close relationship with the lack of a stable biological seal around the transcutaneous parts. Dermal fibroblasts are the main cells in the skin tissue and have been proven to play vital roles in the formation of biological seals. In this work, platelet-rich plasma (PRP), which can release high concentrations of natural cytokines upon activation, was used to stimulate rapid fibroblast growth. Mussel adhesive proteins (MAPs) were used as mediators to anchor the platelets in the PRP onto Ti surfaces, and MAP/PRP composite-coated Ti surfaces were constructed successfully. This in vitro study indicated increased fibroblast adhesion (P < 0.05), spreading, and proliferation (P < 0.05) and upregulated extracellular matrix-related gene expression (P < 0.05) on a MAP/PRP composite-coated Ti surface compared with a control smooth Ti surface. Our results suggest that MAP/PRP composite-coated Ti surfaces are potentially useful for the formation of a stable biological seal in transcutaneous areas.