HCV-Specific Interleukin-21+CD4+ T Cells Responses Associated with Viral Control through the Modulation of HCV-Specific CD8+ T Cells Function in Chronic Hepatitis C Patients

Guo-Hua Feng,Fu-Sheng Wang,Ji-Yuan Zhang,Qing-Lei Zeng,Lei Jin,Junliang Fu,Bin Yang,Ying Sun,Tianjun Jiang,Xiangsheng Xu,Zheng Zhang,Jinhong Yuan,Liyuan Wu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

Interleukin-21 (IL-21)+CD4+ T cells are involved in the immune response against hepatitis B virus (HBV) by secreting IL-21. However, the role of IL-21+CD4+ T cells in the immune response against chronic hepatitis C (CHC) virus infection is poorly understood. This study aimed to investigate the role of IL-21+CD4+ T cells in CHC patients and the potential mechanisms. The study subjects in-cluded nineteen CHC patients who were grouped by viral load (low, < 106 RNA copies/ml, n = 8; high, > 106 RNA copies/ml, n = 11). The peripheral frequency of HCV-specific IL-21+CD4+ T cells was higher in the low viral load group and was negatively correlated with the serum HCV RNA viral load in all CHC patients. Meanwhile, IL-21+ cells accumulated in the liver in the low viral load group. In vitro, IL-21 treatment increased the expression of proliferation markers and cytolytic molecules on HCV-specific CD8+ T cells. In summary, these findings suggest that HCV-specific IL-21+CD4+ T cells might contribute to HCV control by rescuing HCV-specific CD8+ T cells in CHC patients.

MiR-886-5p Inhibition Inhibits Growth and Induces Apoptosis of MCF7 Cells

Zhang, Lei-Lei,Wu, Jiang,Liu, Qiang,Zhang, Yan,Sun, Zhu-Lei,Jing, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.4

Background and Aims: To explore the molecular mechanisms of miR-886-5p in breast cancer., we examined roles in inhibiting growth and migration of MCF-7 cells. Methods: MiR-886-5p mimics and inhibitors were used to express or inhibit MiR-886-5p, respectively, and MTT and clone formation assays were used to determine the survival and proliferation. Hoechst 33342/ PI double staining was applied to detect apoptosis. The expression of caspase-3, caspase-8, caspase-9, MT1-MMP, VEGF-C and VEGF-D was detected by Western blotting, and the levels of MMP2 and MMP9 secreted from MCF-7 cells were assessed by ELISA. MCF-7 cell migration was determined by wound healing and Transwell assays. Results: We found that the growth of MCF-7 cells was inhibited upon decreasing miR-886-5p levels. Inhibiting miR-866-5p also significantly induced apoptosis and decreased the migratory capacity of these cells. The expression of VEGF-C, VEGF-D, MT1-MMP, MMP2, and MMP9 was also found to be decreased as compared to controls. Conclusions: Our data show that downregulation of miR-886-5p expression in MCF-7 cells could significantly inhibit cell growth and migration. This might imply that inhibiting miR-886-5p could be a therapeutic strategy in breast cancer.

Inhibitory Effect of Curcumin on Invasion of Skin Squamous Cell Carcinoma A431 Cells

Wu, Jian,Lu, Wen-Ying,Cui, Lei-Lei Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.7

Objective: To investigate the regulatory effect of curcumin on expression of signal transducer and activator of transcription 3 (STAT3) in skin squamous cell carcinoma tissues as well as possible mechanisms of curcumin in prevention and treatment of skin squamous cell carcinoma. Materials and Methods: Highly invasive A431 cells were treated with curcumin at various doses .The cytotoxic effects of treatment with 5, 10, 15, 20, 25, 30, 35, 40 and 50 umol/L curcumin for 24, 48 and 72 hours on A431 cells were measured by MTT assay. The invasion capacity of cells treated with 5, 10 and 15 umol/L curcumin was measured by Transwell test, while adhesive ability was assessed by cell adhesion assay. The effects of 5,10 and 15 umol/L curcumin on expression levels of STAT3 were determined by Western blotting and on transcription levels of STAT3 mRNA by RT-PCR. Results: Treatment with curcumin at a doses of more than 15 umol/L for more than 24 hour inhibited the growth of A431 cells in a time-and dose-dependent fashion (p<0.001). The doses of 15 umol/L and less for 24 hours showed no significant cytotoxic effects on the cells, survival rates being more than 85%.The invasion and adhesive abilities decreased gradually with the increasing curcumin concentration, 15 umol/L exerting the strongest inhibitory effects (p<0.05). Curcumin showed significant dose-dependent inhibitory effects on the transcription level of STAT3 mRNA (p<0.05). Conclusions: Curcumin may reduce the invasive ability of A431 cells by inhibiting the activation of STAT3 signal pathway and expression of STAT3 as a target gene in the pathway.

Improved DV-Hop Localization Algorithm Based on Distance Correction of Anchor Nodes

Lei Wu,Zhongwei Hou,Can Tan,Dengyuan Xu 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.10

This paper presents a kind of DV-Hop localization algorithm based on distance correction of nodes. This algorithm introduces the concept of distance correction factor and combines with the node communication radius to correct the average distance of each hop between unknown node and anchor node,and makes it be closer to the real value. The simulation results show that the proposed algorithm can reduce the average localization error of node, and when the value of distance correction factor is 0.8,the average localization error of node under DA-DV-Hop localization algorithm is reduced 15.53% than the traditional DV-Hop localization algorithm.

CHISELED NICKEL HYDROXIDE NANOPLATES GROWTH ON GRAPHENE SHEETS FOR LITHIUM ION BATTERIES

LEI-LEI TIAN,XIAN-YONG WEI,QUAN-CHAO ZHUANG,CHAO WU,RUI-LUN XIE,ZHI-MIN ZONG,YONG-LI CUI,SHI-GANG SUN 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.6

The morphologies and structures of Ni(OH)2–graphene hybrid materials were tailored by using different mineralizers in this work. It was revealed that the synergic effects of the highly oxidized graphene sheets and the mineralizers played a crucial role in controlling the morphology and structure of the nanocomposites, and Na2CO3 is a very effective mineralizer for growing chiseled 2D nanoplates of Ni(OH)2 on graphene sheets. When produced with NaOH, fragmental Ni(OH)2 crystals with irregular shapes erratically decorated on graphene sheets. In contrast, chiseled Ni(OH)2 hexagonal nanoplates grown on graphene sheets were obtained when Na2CO3 was used as the mineralizer. These unique 2D–2D nanoarchitectures with higher contact area between the nanocrystals and graphene substrate can increase the interfacial interaction and then efficiently improve the structural stability of the composite material, thus exhibiting an enhanced Li storage capacity and excellent cycling performance of 562 mAh g-1 after the 36th cycle.

Screening and Characterization of Oleaginous Microalgal Species from Northern Xinjiang

Wu, Lei,Xu, Liangliang,Hu, Chunxiang The Korean Society for Microbiology and Biotechnol 2015 Journal of microbiology and biotechnology Vol.25 No.6

A total of 646 strains, including green algae and diatoms, were isolated from 220 samples to screen microalgae with high lipid productivity (LP). The samples were obtained from nine habitats in Northern Xinjiang, China in June 2013. This study initially identified eight lipidrich strains, namely, Desmodesmus intermedius XJ-498, D. intermedius XJ-145, D. intermedius XJ-99, Monoraphidium pusillum XJ-489, M. dybowskii XJ-435, M. dybowskii XJ-151, Mychonastes homosphaera XJ-488, and Podohedriella falcata XJ-176, based on 18S rDNA sequencing. The strains were cultured in a photobioreactor for the same period. Results showed that the specific growth rate (day^-1) of M. pusillum XJ-489 was the highest (1.14 ± 0.06), and the biomass concentration (g/l) of D. intermedius XJ-99 was the highest (2.84 ± 0.3). Futhermore, the lipid content (%) of M. dybowskii XJ-151 was the highest (33.5 ± 4.38), and the lipid productivity (mg l^-1 day^-1) of My. homosphaera XJ-488 was the highest (86.41 ± 9.04). C16 to C18 accounted for 86% to 98% of the total lipid, and the biodiesel qualities of the selected algae corresponded to international standards. This study suggests that My. homosphaera XJ-488, D. intermedius XJ-99, and M. dybowskii XJ-151 are the most potential strains for biodiesel production among all the isolated strains.

Optimizing Conditions for Antioxidant Phenolic Compound Extraction From Mahonia bealei (Fort.) Carr. Leaves Using a Response Surface Methodology

Lei Wu,Gongcheng Wang,Ting Shen,Long You,Weicheng Hu,Chuan-Ling Si 한국원예학회 2017 Horticulture, Environment, and Biotechnology Vol.58 No.3

Mahonia bealei (Fort.) Carr., is an economic plant cultivated in Southwest China commonly used in traditionalChinese medicine. In this study, a response surface methodology was used to optimize experimental conditions forthe extraction of phenolic compounds from the leaves of Mahonia bealei (Fort.) Carr. The highest extraction ratio ofphenolic compounds yielded 39.1 mg gallic acid equivalent/g of dry weight using an ethanol concentration of 30%(v/v) as a solvent and a liquid-to-solid ratio of 21:1 (mL·g-1) for 2 h in 63°C. The crude material was extractedunder optimal conditions, enriched, and then purified through a D-101 macroporous adsorption resin, giving a phenoliccompound-enriched fraction we named TPMB. Evaluation of its in vitro antioxidant activity suggested that TPMBsignificantly scavenged the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, superoxide radical, and hydrogen peroxidein a concentration-dependent manner. In addition, TPMB also exhibited a strong reducing ability and provided protectionagainst oxidative damage induced by oxidative stress in cellular antioxidant activity assays. The results from this studyindicate the suitability of the response surface methodology in optimizing the solvent extraction of phenolic compoundsfrom M. bealei. Further research showed that TPMB possesses a strong anti-radical activity and may be an effectiveoxidation resistance treatment in the medical and food industries.

Identification of novel rheumatoid arthritis-associated MiRNA-204-5p from plasma exosomes

Wu Long-Fei,Zhang Qin,Mo Xing-Bo,Lin Jun,Wu Yang-Lin,Lu Xin,He Pei,Wu Jian,Guo Yu-Fan,Wang Ming-Jun,Ren Wen-Yan,Deng Hong-Wen,Lei Shu-Feng,Deng Fei-Yan 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Rheumatoid arthritis (RA) is an autoimmune disease characterized by infiltration of immune cells in the synovium. However, the crosstalk of immune cells and synovial fibroblasts is still largely unknown. Here, global miRNA screening in plasma exosomes was carried out with a custom microarray (RA patients vs. healthy controls = 9:9). A total of 14 exosomal miRNAs were abnormally expressed in the RA patients. Then, downregulated expression of exosomal miR-204-5p was confirmed in both the replication (RA patients vs. healthy controls = 30:30) and validation groups (RA patients vs. healthy controls = 56:60). Similar to the findings obtained in humans, a decreased abundance of exosomal miR-204-5p was observed in mice with collagen-induced arthritis (CIA). Furthermore, Spearman correlation analysis indicated that plasma exosomal miR-204-5p expression was inversely correlated with disease parameters of RA patients, such as rheumatoid factor, erythrocyte sedimentation rate, and C-reactive protein. In vitro, our data showed that human T lymphocytes released exosomes containing large amounts of miR-204-5p, which can be transferred into synovial fibroblasts, inhibiting cell proliferation. Overexpression of miR-204-5p in synovial fibroblasts suppressed synovial fibroblast activation by targeting genes related to cell proliferation and invasion. In vivo assays found that administration of lentiviruses expressing miR-204-5p markedly alleviated the disease progression of the mice with CIA. Collectively, this study identified a novel RA-associated plasma exosomal miRNA-204-5p that mediates the communication between immune cells and synovial fibroblasts and can be used as a potential biomarker for RA diagnosis and treatment.

Application of RBF Neural Network in Fault Diagnosis of FOG SINS

Wu Lei,Sun Rong-Ping,Cheng Jian-Hua 제어로봇시스템학회 2008 제어로봇시스템학회 국제학술대회 논문집 Vol.2008 No.10

Taking FOG SINS (fiber-optic gyroscope strapdown inertial system) as an object, a new fault diagnostic scheme based on RBF(radial basis function) neural network is proposed. Being capable of training and simulating data off-line, neural networks provide a solution to overcome some drawbacks of the quantitative fault diagnosis. The fault tree of FOG SINS is analyzed, which is the basis of the study of neural network fault diagnosis technology. The structure and inferential mechanism of RBF network used for elementary fault diagnosis are discussed in detail. Training simulation results of the neural network are given and an improved effect with real data is obtained, which show the feasibility of the proposed scheme.

신도식과 관념의 존재 가치 - 당대 표현예술의 사명 탐구 -

( Wu Lei ),( Di Dongzhan ) 인문사회과학예술융합학회 2020 인문사회과학예술융합학회지 Vol.4 No.2

Explore the mission of contemporary expressive Art -- What is conveyed to people and passed on to The Times? It is the embodiment of the existential value of the new schemata and concepts in contemporary expressive art. With new eyes to see the contemporary performance art, swift capture, listening, feeling the demands of our era, and suggests that to explore the time background of works creation, have the courage to explore something useful from the text, the understanding of contemporary art of the new concept of schemata and harmony within, hard practice may harvest is unpredictable. Because the history of art is not the history of technical schemata proficiency, but can be understood as the history of changes of artistic concepts and artistic expression schemata to meet the needs of The Times. We are a nation with a long history. How to implement the civilization art of thousands of years in the current world and to establish the memory of the future is the mission that The Times endow contemporary artists with inheritance and creation.