병재배 폐톱밥을 이용한 양송이 복토재료 개발에 관한 연구

김홍규,이희덕,김용균,한규홍,문창식,김홍기 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

애느타리 병재배후 탈병한 톱밥을 3월 중순 퇴적하여 부숙시킨 결과 퇴적기간에 따른 폐톱밥의 부숙정도는 퇴적 30일이후에 가장 컸으며 부숙 소요일수는 최소 48일이었다. 퇴적 기간에 따른 폐톱밥의 pH 및 총 질소 함량은 부숙이 진전됨에 따라 증가하는 경향이었고 총 탄소 및 C/N율은 시간이 경과함에 따라 감소하는 경향이었다. 식양토에 부숙이 완료된 톱밥을 부피 비율로 10, 30, 50%씩 첨가하여 복토 재료로 사용한 결과 30% 첨가시 식양토 단용 처리보다 A. bisporus 균사 생장이 촉진되고 초발이 소요일수가 5일 빠르며, 수량에 있어서도 28% 증수되었다. After bottle culture of Pleurotus ostreatus, sawdust was taken out from the bottle and accumulated in the middle of March, and then composted. As the result, Y value was decreased rapidly 30 days after composting, and it was decreased slowly after 30 days. It is considered that 118 days is required for composting, however, it is possible to use for casing material after at least 48 days composting. The pH and total nitrogen content of sawdust based on composting period had tendency to increase as composting was processed. Total carbon and C/N rate had tendency to decrease as time went on. Based on the rate of 10, 30 and 50%, each sawdust was added to clay loam used as casing material for culturing A. bisporrus. Among various treatments, the mycelial growth of A. bisporus was more favorable in the treatment of 30% sawdust than in the single treatment of clay loam. Based on the date necessary for primodium formation of A. bisporus, the primodium formation in the treatment of 30% sawdust was reduced to about 5 days as compared with that of any other treatments When 30% sawdust was added to clay loam used as casing material for culturing A. bisporus, the yield of its fruiting body was increased to 28%.

Cisplatin과 Tobramycin이 白鼠 Corti器에 미치는 영향에 關한 電子 顯微鏡的 硏究 : Ultrastructural Study

김동환,신태균,김무강,김홍선,김원식 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

In order to study the morphological changes of Corti’s organ in the rat treated with cisplatin and tobramycin, thirty-three rats, weighing between 200∼250g, were used, and divided into 4 groups, i.e., 3 rats were used for control, and 30 rats were used for 3 experimental groups. Each experimental group was divided into cisplatin-treated, tobramycin-treated, and both cisplatin and tobramycin treated group with 10 rats. Rats were injected with a single dose of 7.7㎎/㎏ of cisplatin intraperitoneally and 50㎎/㎏/day of tobramycin for 2 days intramuscularly, respectively or both. Treated rats were sacrificed on the 3rd and 5th day after the first injection. Results were as follows; 1. Cisplatin-treated group: Outer hair cells of Corti’s organ were degenerated with the formation of vacuoles and appearance of lysosomes, however, inner hair cells showed mild vacuolization. 2. Tobramycin-treated group: NO pathological changes were found in Corti’s organ. 3. Combined cisplatin and tobramycin treated group: Outer and inner hair cells showed vacuolization, appearance of lysosomes, myelin figures and multivesicular body. There were also multivesicular body in the inner phalangeal cell and myelin figure in the Reissner’s membrance. 4. Degeneration of Corti’s organ in the combined treated group was more sever than those of the single cisplatin-treated or tobramycin-treated group. 5. NO pathological differences of Corti’s organ in duration of postinjection were manifested in any groups. It can be concluded that cisplatin induces toxicity to inner ear and cisplatin enhance toxicity more in continued treated with tobramycin.

가토에서 일측 요관 부분 및 완전폐색시 신장과 요관의 조직학적 변화

김상현,김용웅,육승모,한동석,노안식,임재성,김홍식,나용길,설종구 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

This study was designed to clarify histologic changes in the experimental rabbit kidney and ureter after unilateral partial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, the partial obstruction followed by 4 weeks, the complete obstruction followed by 2 weeks, and the complete obstruction followed by 4 weeks. The normal control group was consisted of 5 adult white rabbits. The kidneys and ureters for light microscopy were fixed in 10% neutral formalin, embedded in paraffin and stained with hematoxylin and eosin. Abnormal histologic fingings showed in ipsilateral kidney and ureter of all subgroups(such as dilatation of uriniferous tubule, focal necrosis and desquamation of epithelium, interstitial fibrosis, glomerular congestion, widening of Bowman's space, dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis and interstitial inflammation). These findings were most severe in the group of the complete-obstructed ureter for 4 weeks. No significant changes showed in contralateral ureter of all subgroups. The degree of obstruction was severe and period of obstruction was long, the histological changes in ipsilateral side were severe. Despite of changes in ipsilateral side no abnormal findings showed in contralateral side. We suggest that long term experimental investigation of histologic changes in ipsilateral side and compensatory changes in contralateral side have to be studied.

비만 개선 효과를 지닌 탄수화물 및 지방 흡수 억제 기능성 식이조성물 개발

윤유식,최선미,홍순복,홍정미,김정원,이홍석,홍성길 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.3

In a previous study, we developed a new food additive as an egg yolk antibody (IgY) against carbohydrate digestion enzymes for the regulation of blood glucose level and weight control. The IgY delayed and decreased the increment of blood glucose level after administration of sucrose in human being by 30% in 20∼30 min. We also developed a lipase inhibitor as a water extract of two kinds of herb, Platycodon grandiflorum and Solanum melongena. Twenty three volunteers were subjected to the intake of the egg yolk IgY plus the herbal extracts for 50 days. In average, the treated subjects appeared to lose 1.96 ㎏ of body weight and 3.4 ㎏ of body fat mass during the treated period. Furthermore, Panniculus adiposus and breech size were significantly decreased during the experimental period. Above results suggested that the administration of the dietary additives composed of egg yolk IgY and natural herbal extract improve the obesity by the decrement of body weight and body fat mass.

가토에 있어서 편측 요관 폐색시 환측, 반대측 요관에 대한 변화 : 조직학적 소견을 중심으로

설종구,김용웅,육승모,노안식,김홍식,김상현,이충식 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

This study was designed to clarify histological changes in the experimental rabbit ureter after unilateral prartial or complete ureteral obstruction. A total of 20 adult white rabbits were divided into 4 subgroups in the partial obstruction followed by 2 weeks, partial obstruction followed by 4 weeks, complete obstruction followed by 2 weeks, complete obstruction followed by 4 weeks. The normal control group consisted of 5 adult white rabbits. The H & E stain. light microscopy were used. Abnormal histological fingings were showed in ipsilateral ureter of all subgroups; dilatation of ureter, epithelial cell atrophy or loss, interstitial fibrosis, interstitial inflammation. These findings were most severe in the complete-obstructed ureter for 4 weeks. There were no significant changes in contralateral ureter of all subgroups. Despite of progressive histological changes in ipsilateral ureter no abnormal findings were showed in contralateral ureter. We suggest that long term experimental investigation for histological changes in ipsilateral ureter and compensatory changes in contralateral ureter should be done.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

다발성 결절양상의 피부전이로 발견된 췌장암 1예

김희수,이상표,박성우,류성태,오웅식,이정환,김홍자 대한내과학회 2004 대한내과학회지 Vol.66 No.4

췌장암은 악성종양의 5% 이하를 차지하며, 피부전이는 매우 드물다. 저자들은 결정성 전신피부병변으로 내원한 환자에서 초음파와 컴퓨터단층 촬영상 췌장종괴가 발견되고 CA 19-9이 매우 높았으며 피부조직 검사를 시행한 결과 전이성 선암으로 판정되어 췌장암에서 유래된 피부전이암으로 진단되어진 증례를 접하고 문헌고찰과 함께 보고한다. Carcinoma of the pancreas represents less than 5% human malignant neoplasm. It's skin involvement is rare. A 73 year-old man presented with multiple skin lesions located on the face, neck and body. The histopathologic study of the skin lesion was confirmed to be an metastatic adeno-carcinoma. The primary tumor was regarded as the mass at the head of the pancreas observed on the CT scan, but was not confirmed by biopsy. We report a case of multiple cutaneous metastasis from pancreatic carcinoma with brief review of related literature.

Dexamethasone이 부신피질에 미치는 영향에 관한 연구

金相吾,李永浩,金源式,金弘善 충남대학교 의과대학 지역사회의학연구소 1988 충남의대잡지 Vol.15 No.2

For the morphological study of dexamethasone upon adrenal cortex, dexamethasone was injected intramuscularly into the leg of the ICR mice by the dose of 100 ㎍/day and 500 ㎍/day for 10 days, and observed the morphological changes of the adrenal cortex stained with PAS and oil red 0. The results were as followings. 1. The total thickness of the adrenal cortex of the group injected ly dose of 100 ㎍/day of dexamethasone was 73.2% of the thickness of normal group, and that of the group injected by dose of 500㎍/day of dexamethasone was 54.1% of the thickness of normal group. Decrease of total thickness of the adrenal cortex of the group injected by dexamethasone was due to decrease of the thickness of zona fasciculata. 2. The cross-sectional area of the cell of the group injected by dose of 100 ㎍/day of dexamethasone was 74.5% of the cross-sectional area of normal group, and the cross-sectional area of the group injected by dose of 500 ㎍/day of dexamethasone was 47.3% of that of normal group. But, cross-sectional area of the cell of zona glomerulosa and zona reticularis was not significantly different normal group from the injected group. 3. The lipid droplets of the zona fasciculata of normal group were round and the cross-sectional area were around the area of the cell of this layer. Those of the group injected by dose of 100㎍/day of dexamethasone decreased than normal group and the cross-sectional area of main lipid .droplets were around the area of the nucleus of the zona fasciculata cell. And those of the group injected by dose of 500㎍/day of dexamethasone were decreased than the group injected dose of 100 ㎍/day of dexamethasone and the cross-sectional area of the main lipid droplets of the group were much smaller than the nuclei of the zona fasciculata cells.

「鋏」語에 대한 小考 : 特히 濟州島 方言과 關聯해서 In Relation to the Cheju-Do Dialect

金洪植 제주대학 1975 논문집 Vol.7 No.1

The oldest mention of the word stem "Kawi" was made in the book "Kyerim Yysa" which was written in the early part of the 12th century. In this paper, the writer attempts to offer some suggestions as to how to interpret the word stem in relation to the Cheju-Do Dialect. The perfect interpretation of the stem and its phrases will have to wait for the explication of the then pronunciation of the Chinese characters, the collection of other literature to back these pronunciation and the study of the contemporary dialects. Japanese scholars including Rokuro Kono, however, already presented their papers on this subject and the writer, too, attempts to interpret the stem and its phrases. The writer has come to believe that the word stem "Kawi" is the same stem found in [kbsbgε] of the Cheju-Do Dialects. In the Cheju-Do Dialects, [kbsw-] is still used to convey the meaning of "cutting" and "nipping" This old form disappeared from the mainland dialects long ago but there are reasons to believe that it once existed as a linguistic stem and that it underwent a phonetic change to turn into [kbsb-] because of "vowel harmony" around the 15th century. This transformed stem [kbsb-] survived in this geographically isolated island and attracted [gε] as a suffix. Since Korean and Manchu belong to the same linguistic family, the two languages, it is supposed, had the same parent and common stems for some ages before they diveraged into Korean, Manchu and Japanese.

經皮神經 電氣刺戟이 β-Endorphin 및 Cortisol値에 미치는 影響

김홍열,김완식 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.1

A great deal of efforts in the past few years related to the synthesis, Degradation and mapping of opiate receptors and the relation of β-endorphin to other neurotransmitter systems. Recenly major efforts have have been undertaken to develop assays for β-endorphin and cortisol in plasma to study possible effects of pain, stress, diural variation and activity. It was a well known fact that β-endorphin and cortisol were secreted concomitantly by the pituitary gland. However, researchers have noted an increase or decrease of β-endorphin and cortisol in pasma after Transcutaneous Electrical Neve Stimulation (=TENS), and there may or may not be the correlation between acute and chronic pain. This study has investigated that changes of β-endorphin and cortisol levels after TENS in the chronic pain(n=12) and normal healthy volunteer subjects(n=12). We measured β-endorphin levels with ?? RIA KIT and cortisol levels with ?? cortisol Immunoassay KIT, and also observed the changes of blood pressure and pulse rate before TENS, at 30 minutes and at 60 minutes after TENS. The results were as follows: 1. There were no significant differences on age, height and weight in compared with groups. 2. The pain intensity was markedly improved in the experimental group after TENS. 3. The canges of blood pressure and pulse rates were not statistically significant in both groups after TENS. 4. The changes in β-endorphinlevel were oberved with a sight increase or devrease in both groups after TENS, but there were no constant tendency. 5. The changes in the cortisol levels were observed with a slight cedreasing tendency in both groups after TENS but there were no signigicant changes in both groups. These results show that the relief of pain intensity with TENS were not correlated with β-endorphin and cortisol levels.