Studies on the Activation Mechanism of c-src Protein Tyrosine Kinase by Ginsenoside-Rg1

Hong, Hee Youn,Yoo, Gyurng Soo,Choi, Jung Kap 전남대학교 약품개발연구소 1998 약품개발연구지 Vol.7 No.1

We have studied an activation mechanism of pp60^(c-src) protein tyrosine kinase (PTK) by ginsenoside-Rg₁ (G-Rg₁) in NIH(pMcsrc/foc)B c-src overexpressor cells. It was previously reported that G-Rg₁ stimulated the activation of c-src kinase at 20 μM with a 18 hr-incubation, increasing the activity by 2-4-fold over that of untreated control, and this effect was blocked by treatments of inhibitors of either protein synthesis (cycloheximide) or RNA synthesis (actinomycin D) (Hong. H.Y. et al. Arch. Pharm. Res. 16, 114 (1993)). However, an amount of c-src protein itself in wild-type cells was not changed by G-Rg₁. When the cells mutated at one or two tyrosine residue(s) (Y416/527) that are important sites to regulate the kinase activity were treated with G-Rg₁, increases both in the activity of c-src kinase and in the expression of the protein were not observed. In addition, removal of extracellular calcium ion by EGTA or inhibition of PKC by H-7 canceled the G-Rg₁-induced activation of the kinase. Although the activation was little affected by G-Rg₁ with a calcium ionophore A23187, it was synergistically stimulated by treatment of G-Rg₁ and PMA, a PKC activator. Taken together, these results suggest that the activation of c-src kinase by G-Rg₁ is caused by an increase in the specific activity of the kinase, but not in amount of it, and is involved with both cellular calcium ion and PKC. Further the increase in the specific activity of c-src kinase may result from altered phosphorylation at tyr-416 and -527.

Sezary증후군과 병발된 용혈성 빈혈 1예

유광하,진춘조,김홍승,이종인,심영학,정순희,이창훈 건국대학교 의과학연구소 1997 건국의과학학술지 Vol.7 No.-

저자등은 전신적인 소양감을 동반한 홍반성 판상의 피부 병변과 자가 면역성 용혈성 빈혈이 동반된 Sezary 증후군을 진단하고 복합 화학 요법 CHOP으로 치료하여 피부 증상과 빈혈이 호전된 예를 문헌고찰과 함께 보고하였다. Sezary syndrome(SS), a leukemic variant of cutaneous T-cell lymphoma(CTCL), is monoclonal proliferation of CD4 T-cell having cerebriform nuclei in the skin and the peripheral blood. It is associated with erythroderma, pruritis, lymphadenopathy with protracted clinical course and systemic spread. We present a case of SS i a 38-year-old woman with generalized exfoliative erythroderma, plaques, and pruritus. Histopathologic findings of the skin lesions revealed infiltration of atypical lymphocytes in the dermis and epidermis. Electron microscopic findings of peripheral blood and skin showed atypical lymphocytes with cerebriform nuclei. Laboratory findings revealed direct Coombs' positive autoimmune hemolytic anemia, a rare association of SS. Chemotherapy with CHOP produced improvement of the skin manifestation and anemia.

민들레 추출액 농도에 따른 민들레 코팅쌀밥의 품질에 관한 연구

유경미,이연경,김세희,황인경,이부용,김성수,홍희도,김영찬 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1

Washed rice was coated by spraying aqueous dandelion (Taraxacum officinale) extracts at 20, 30, 40 'Brix and dried at room temperature. The coating procedure was conducted 5 mL/min for 10min. Property changes in the dandelion coated rice and un-coated cooked rice were observed during 2 days of storage. Increasing the coating concentration was associated with lower color values (L, a value) and moisture contents. Sensory and texture properties generally decreased in the cooked rice coated with dandelion more than the control (non-coated rice). But, D-20(20 °Brix of the dandelion concentration) resulted in better textural properties and s ensnry scores for hardness and overall acceptability in cooked rice. Compared to the control, the washed rice roated with 20 °Brix for 0 and 2 days showed better quality in sensory activities, a lower gel consistency and better textural activities. It was concluded that the optimum coating ration of rice and added dandelion extract for cooking were 20 °Brix and 5% respectively, in proportion to the total weight of raw rice.

중추신경자극제를 사용할 수 없는 주의력결핍과잉행동장애 아동에서의 Modafinil 효과 : 예비 연구

유한익,김진용,박수진,전정원,왕희령,김붕년,홍진표,김성윤,김창윤 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.6

Objectives : This study was aimed to examine the efficacy and safety of modafinil in psychostimulant-inapplicable children with attention deficit hyperactivity disorder (ADHD) due to insufficient treatment response or intolerable adverse effects. Methods : Fifteen children (8.8±1.8 years, 14 boys, total IQ 107.22±13.4) were recruited for this study. ADHD was confirmed by K-SADS-PL. The efficacy was assessed by ADHD rating scale (ADHDRS), Conner's parental rating scale-revised(CPRS-R), ADHDRS school version, clinical global impression (CGI) and computerized neurocognitive function test. Mean dose of modafnil was 250. 0±103.3 mg. Results : Total score (p=0.012) and hyperactive/impulsive score (p=0.021) of ADHDRS for investigator, as well as CPRS-R score (p<0.01) and CGI-severity score (p<0.01) were significantly reduced after 39.2±8.7 days of modafmil administration. Response time of word color test was also significantly decreased after medication. Decreased appetite (2 subjects), insomnia (2 subjects), irritability (1 subject) and alopecia (1 subject) were observed during this study period. Conclusion : Modafinil was effective and well-tolerated for the psychostimulant-inapplicable children with ADHD.

IMF에 관련된 경제적 스트레스가 정신 건강에 미치는 영향

유희정,황성훈,홍진표,김헌수,이철,한오수 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.1

본 연구는 IMF에 관련된 경제적 스트레스가 정신 건강에 미치는 영향과 그 과정을 밝히기 위해 설계되었다. 스트레스 과정에 관여하는 요인으로 사회적 지지와 문제 해결 양식, 그리고 무망감을 도입했으며, 이들에 의해 스트레스의 영향이 매개되는지를 검증하였다. 경제적 스트레스의 수준에 따라 표집된 221명의 일반인들을 대상으로 우울, 불안, 신체화, 적대감, 사회적 지지, 그리고 문제 해결 양식 등을 측정하였다. 가정한 대로 실직으로 대표되는 경제적 스트레스는 정신 건강에 부정적 영향을 미치는 것으로 나타났다. 그 구체적인 양상을 살펴보면 다음과 같다. 먼저, 사회적 지지는, 높은 스트레스 조건에서 증상의 발현을 억제하는 완충 효과가 있음이 입증되었다. 두번째로, 남성이 여성에 비해 경제적 스트레스의 부정적 영향에 더 취약한 것으로 나타났다. 세번째, 경제적 스트레스는 문제 해결 양식의 다양한 구성요소중 문제 해결에 대한 무기력감에만 영향을 미치는 것으로 나타났다. 네번째, 무망감은 경제적 스트레스와 정신 건강간의 관계를 매개하는 변인임이 인과모형 검증을 통해 확인되었다. 끝으로, 이러한 결과가 스트레스 개입 프로그램 개발을 위한 이론적 기초를 제공한다는 의의가 있음을 논의 했고, 본 연구의 몇 가지 한계점을 지적하였다. This study was designed to investigate the influence of the so-called IMF-related economic stress upon the mental health and to analyze the process of the influence. As the possible variables moderating the stress process, social resource, problem solving style, and hopelessness were included. 221 male and female subjects were recruited according to the intensity of economic stress and classified into four groups(employees of solid companies, employees of the companies at risk, short-term unemployed and long-term unemployed). Relevant measures including economic stress scale, selected SCL-90R subscales(somatization, depression, hostility, anxiety), social support scale, problem-solving scale, and job hopelessness scale were administered. As assumed, it was proved that economic stress had adverse effects on the mental health. In addition to this basic causal relationship, moderating effects of several variables were found as follows: First, social support had the buffering effect upon the economic stress. That is, strong social support played the role suppressing the development of psychiatric symptoms under high stress. Second, men were more vulnerable to the adverse effects of economic stresses than women. Third, economic stresses had influence on the helplessness concerning the problem solving. However other problem solving strategies such as creative style, approach style, avoidance style, etc were proven to have no relationship with the economic stress. Fourth, in the analysis of unemployed group, it was found that hopelessness mediates the relationship between economic stress and mental health. In conclusion, the clinical implications of such findings in economic stress process were discussed and several limitations and the suggestions for future researches were also addressed.

경막 내 colistimethate 투여로 치료한 다제 내성 cinetobacter baumannii 뇌실염

홍유아,유진홍,김진진,모은영,안건희,정희경,김진석,이현정,정미향,윤승배 대한감염학회 2009 감염과 화학요법 Vol.41 No.4

Acinetobacter species is a non-fermentative aerobic gram-negative coccobacillus that is an important pathogen found in nosocomial infections. Recently, multi-drug resistant Acinetobacter baumannii (MDR-AB) infections have been increasing and pose a serious problem. Most such infections present as bacteremia, pneumonia, or a wound infection; however, CNS infections are very rare. We herein present a case of ventriculitis caused by MDR-AB in a 37-year old man after a neurosurgical intervention. The patient was successfully treated with intrathecal colistimethate.

노인 낮병원의 심리사회적 치료 프로그램이 노인의 신경인지기능과 우울 증상에 미치는 효과

유계준,송정은,오병훈,안석균,이홍식,오희철,구은형,황혜숙,이은철 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

노인에게 있어서 신경인지기능의 저하와 우울 증상은 가장 흔한 장애이며 신체적, 정신적인 면에 많은 영향을 미치고 있다. 또한 이러한 장애중 비임상군에서 심리사회적 치료개입의 효과가 보고되고 있다. 이러한 측면에서 지역사회에서 노인에게 제공되는 심리사회적 치료개입의 중요성이 높아지고 있다. 이전의 연구에서는 대상이 특정 집단이거나, 비교군이 없거나, 치료효과에 대한 추적 관찰이 이루어지지 않는 등의 제한점이 있었으며, 현재 국내의 노인 낮병원은 소수에 불과하고 이러한 낮병원의 치료효과에 대한 연구는 거의 없는 실정이다. 따라서 본 연구는 지역사회 정신보건센터에서 운영하는 노인 낮병원에 참가하는 노인들을 대상으로 낮병원의 심리사회적 프로그램이 노인들의 신경인지기능과 우울 증상에 미치는 효과와 이에 대한 유지 효과의 유무를 알아보고자 하였다. 경기도 광주군 정신보건센터의 노인 낮병원에 참여한 노인을 치료군으로, 치료를 거부하거나 치료 중 탈락한 노인을 비교군으로 하여 10주간의 낮병원 치료전후와 치료종결 후 10주에 신경인지기능은 한국형 간이 정신상태검사(이하 MMSE-K)로, 우울 증상은 단축형노인우울척도(이하 SGDS)로 평가하여 두 군을 비교하였고 치료군의 치료 직후와 치료종결 후 10주를 비교하였다. 연구결과 노인 낮병원의 심리사회적 치료가 시간에 따른 SGDS점수 변화에 통계학적으로 유의한 영향을 미쳤으며, MMSE-K점수 변화에는 통계학적으로 유의한 영향을 미치지 않았다. 또한 10주 추시가 가능했던 치료군 중 약 43%가 10주 후 SGDS가 증가하여 호전되었던 우울증상의 악화를 보였다. 본 연구 결과 노인 낮병원의 심리사회적 치료 프로그램 개입은 노인에게 있어서 우울 증상을 호전시키며 신경인지 기능에는 직접적인 영향을 미치지 않는 것을 알 수 있었다. 또한 상당수에서 심리사회적 치료 개입으로 인한 우울 증상의 호전이 유지되지 않음을 알 수 있었다. 이는 노인우울증상의 치료에 낮병원의 심리사회적 치료 프로그램이 효과적일 수 있음과 치료 후 이를 유지하기 위한 부가적인 치료개입의 필요성을 시사한다. Cognitive impairment and depression are the most common symptoms affecting the elderly on physical and mental states. Studies have shown that these symptoms in mild cases are improved by psychosocial intervention, emphasizing the importance of therapeutic intervention provided in the community. The purpose of this study was to investigate whether the psychosocial program at a geriatric day care service has any therapeutic effect on the cognitive function and depressive symptoms in the elderly and whether such effect can be maintained. The experimental group was composed of 37 elderly patients attending the day care service at the mental health center located in Kwangju county, Kyonggi province. The control group was composed of 22 elderly patients who either refused intervention or who dropped out of the program. Cognitive function was assessed with the Korean version of the Mini-Mental State Exam(MMSE-K), and depressive symptoms were assessed with the Short-form Geriatric Depression Scale(SGDS). The experimental group and the control group were compared on the score of on each test, which was administered before and after the program and 10 weeks after discontinuation of program. In the experimental group, the scores acquired immediately after the program and at 10 weeks of follow-up were also compared. The results showed that psychosocial intervention at a geriatric day care service was significantly associated with the change in SGDS scores dependent on time but not significantly associated with the change in MMSE-K scores. In the experimental group whose follow-up assessment was possible, it was further shown that 43% of patients had increased SGDS scores 10 weeks after the program ended, indicating that depressive symptoms had worsened. This study suggests that psychosocial therapeutic intervention in the geriatric day care service improve depressive symptoms but not cognitive functions in the elderly. In addition, for a considerable percentage of subjects in the experimental group, the improvements in depressive symptoms were not sustained after the intervention was withdrawn. These findings proposes a need of strengthening therapeutic intervention to maintain such effect.

거대 점오염원이 주변 대기질에 미치는 영향에 관한 연구

김유근,이화운,전병일,장은숙,홍정혜,문윤섭,원경미,송정희 부산대학교 환경문제연구소 1996 環境硏究報 Vol.14 No.1

In order to show the effect of a vast point pollutant source on air quality of Pusan Thermoeletric Power Plant and its surrounding area, air quality around Pusan Thermoeletric Power Plant was simulated by ISCLT-2 which was supplied by EPA. For this purpose the emission amount of SO_2, NO_2 and TSP was calculated and atmospheric stability was classified for a recent decade(1985~1994) in Pusan. A result of the emission amount showed that much amount of NO_2, NO_2 and TSP are emitted from industrial area. It was clear that NO_2 is much emitted from line source and industrial area. And as a result of classification of atmospheric stability, neutral, stable and unstable state were 58%, 24.1% and 17.9%, respectivly. The result of ai quality simulation by ISCLT-2 showed that Pusan Thermoeletric Power Plant is affecting on the increse of 2.0ppb, 3.0ppb and 5.0㎍/㎥, SO_2, NO_2, and TSP respectively at its surrounding area, site A-3 which was located westward 2.2㎞ distance from Plant

광주시내 업종별 음식점의 주방기구와 관련된 주요 세균 및 이들의 계절별 변화

이홍열,유맹자,정해진,김근영,정희종 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

업종별 음식점에서 사용중인 주방기구 가운데 개숫물중에 존재하는 세균들을 분리·동정한 결과, 한색음식점에서 37균주, 일식음식점에서 23균주, 및 중식음식점에서 33균주가 분리되었고 이들 중 한식음식점에서 E. coli 등 16균주, 일식음식점에서 Listeria sp. 등 20균주, 중식음식점에서 Micrococcus sp. 등 15균주를 각각 동정하였다. 이들 세균들은 업종별 음식업소에서 주로 사용되는 색품재료에 존재하는 세균들과 밀접한 관계가 있는 것으로 밝혀졌고 E.coli와 같은 일부 세균들은 주방의 불결한 환경이나 종사자로부터 오염된 것으로 추정되었다. 업종별 음식점에서 사용중인 개숫물, 행주, 칼, 및 도마에 존재하는 세균의 분포는 일반세균수 및 대장균수가 모두 2월에서 8월까지 점점 증가하다가 11월에는 다시 감소하는 경향을 보였다. 특히 이같은 경향은 일반세균은 개숫물에서 대장균군은 칼에서 가장 뚜렷하게 나타났다. 따라서 식중독 발생 가능성이 가장 높은 여름철에는 칼, 도마 등 주방기구들은 정기적으로 열탕소독하는 철저한 위생처리가 필수적인 것으로 생각되었다. Thirty-seven, twenty-three, and thirty-three bacterial strains were isolated from dish-washing water which was selected as a kitchen supply commonly loaded the highest level of bacteria among various supplies using at Korean style, Japanese style, and Chinese style restaurants. Among these isolates, sixteen strains including Esherichia coli, twenty strains including Listeria sp., and fifteen strains including Micrococcus sp. were identified from Korean style, Japanese style, and Chinese style restaurants, respectively. This result suggested that most isolated bacteria were closely related to bacterial flora of raw food materials used in each style of restaurants and others could be contaminated from the surrounding environment and the employees. Total and coliform bacterial counts distributed in dish washing water, wiping cloths, knives, and cutting boards used at different types of restaurants were gradually increased from February to August and then decreased until November. This changing tendencies of total bacterial counts and coliform group counts were obvious in dish-washing water and in knives, respectively. Therefore, kitchen supplies such as wiping cloths, cutting boards, and knives should be treated carefully with hot water of above 45℃ at every certain period of time to kill some of these potential hazardous bacteria and to prevent outbreaking foodborne-illness by them, especially during Summer.

신냉매용 자동차 에어콘 시스템에서 팽창밸브의 특성 해석

송유호,박종일,홍희기,김경훈 경희대학교 산학협력기술연구원 1996 산학협력기술연구논문집 Vol.2 No.-

This study was performed to design the optimal Block Type Expansion Valve through analyzing the characteristics of the Block Type Expansion Valve in automotive air conditioning system using HFC-134a. To make easy flow control and pressure drop, the main function of Block Type Expansion Valve, a preliminary experiment on 3 kinds of orifice(Rectangular Type, Diffuser Type, Cone Type) was carried out and the flow characteristics in Expansion Valve was analyzed. Based upon the results of the experiment, 2 types of Block Type Expansion Valve were installed in automotive air conditioning system using HFC-134a and the characteristics of the 2 types of Block Type Expansion Valve were analyzed and compared under variation of compressor RPM.