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Gas Transfer and Hemolysis in an Intravascular Lung Assist Device Using a PZT Actuator
Chul-Un Hong,Jeong-Mi Kim,Min-Ho Kim,Seong-Jong Kim,Hyung-Sub Kang,Jin-Shang Kim,Gi-Beum Kim 한국정밀공학회 2009 International Journal of Precision Engineering and Vol.10 No.1
The purpose of this study was to investigate the effect of multiple mechanical forces in gas exchange and hemolysis in an intravascular lung assist device (IVLAD). Specific focus was given to the effect of membrane vibration We designed the oscillatory type artificial lung assist device attached with a polyvinylidene fluoride sensor and a lead zirconate titanate (PZT) actuator (vibrator). Maximum oxygen transfer occurred at a frequency of 7 ㎐ for bovine blood and 35 ㎐ for distilled water. The normalized index of hemolysis oxygenator values for the circuit was 0.0014 g/㎖ for excitation of the PZT actuator with a sinusoidal 10-V wave and 0.0018 g/㎖ for a 50-V wave. The experiment results indicate effective performance in enhancing the gas transfer of the IVLAD. This novel hollow membrane jilter IVLAD design demonstrates an acceptable level of gas exchange performance with an acceptable level of blood compatibility, and so it has potential as an implantable lung assist device for patients with acute respiratory distress syndrome.
Chul Young Kim,Hee Ju Lee,Eun Ha Lee,Sang Hoon Jung,Dong-Un Lee,Suk Woo Kang,Sae Jin Hong,Byung-Hun Um 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.4
This study employed high performance liquid chromatography (HPLC) coupled to an on-line ABTS<sup>+</sup> radical scavenging detection (RSD) system along with HPLC-electro spin impact/mass spectrometry (ESI/MS), to rapidly determine and identify antioxidant compounds occurring in blueberry extract. The extract was separated by HPLC, and then the radical scavenging activities of the separated compounds were evaluated by the on-line coupled ABTS<sup>+</sup>-RSD system. The negative peaks of the ABTS<sup>+</sup>-RSD system, which indicates the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The active components in the blueberry extract were identified by HPLC-ESI/MS using their MS spectra and retention times. According to the data acquired from the on-line HPLC-ABTS<sup>+</sup>-based assay and HPLC-ESI/MS systems, the antioxidant compounds detected in the blueberry extract were identified as chlorogenic acid and 11 anthocyanins.
Hong, Chul-Un,Kang, Hyung-Sub,Kim, Seong-Jong,Kang, Sung-Jun,Kim, Gi-Beum 한국정밀공학회 2010 International Journal of Precision Engineering and Vol.11 No.3
Recent developments in high-technology experimental equipment and semiconductor micro fabrication technology have made it possible to study various characteristic quantum phenomena such as quantized conductance and coulomb-blockade effects at the nano-scale level. One recent study attempted to fabricate a normal-super-normal double tunneling junction in a split-gate wire. In this study, we describe the results of transmission electron microscopy (TEM) observation of a single 100 nm wide and 200 nm high Ni dot, which had been fabricated using scanning tunneling microscopy (SEM), placed on the top of the bridge of a Si-doped GaAs epitaxial layer grown by molecular beam epitaxy using a Riber 32 apparatus. The results from the successful use of TEM to observe the results of a SEM fabrication procedure support the contention that the fabricated Ni dot became a poly-crystal that was amorphous between GaAs crystal and a surface dot fabricated by applying voltage pulse to a STM tip, covered by an oxygenated film approximately 20 nm thick.
Astaxanthin 처리 산란계로부터 생산된 난황의 Mouse에 대한 Catabolic Response Overcome 효과
김홍출(Hong-Chul Kim),박숙자(Sook-Jahr Park),박철우(Cherl-Woo Park),김영림(Young-Rim Kim),김정환(Jeong-Hwan Kim),최의성(Eui Sung Choi),조현종(Hyeon-Jong Cho),조용운(Yong Un Cho),하영래(Yeong Lae Ha) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.6
Astaxanthin을 산란계에 경구 투여하여 얻은 AEY에는 총 2.88 mg%의 carotenoid가 함유되어 있었다. Carotenoid 성분을 HPLC로 분석한 결과, CEY에는 존재하지 않는 α-cryptoxanthin, canthaxanthin, cynthiaxanthin, triol 등이 AEY에서 확인되었다. CEY에서는 존재하지 않기 때문에 이들은 astaxanthin의 대사생성물로 예상할 수 있다. AEY는 LPS로 유발한 mouse의 catabolic response overcome의 효과 시험에서 control구에 비해 유의성있는 체중 감소 억제 효과를 보였다. 또한 AEY는 LPS 처리에 의한 면역기관에 미치는 영향에서는 췌장과 간의 체중에 대한 상대적 비율을 증가시켰다. 따라서 AEY 처리가 면역작용을 하는 세포의 활성에 관여하는 것으로 생각되고 이것은 AEY 처리구에서 관찰되는 α-cryptoxanthin, canthaxanthin, cynthiaxanthin, triol 등에 의한 것으로 추측된다. Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on the catabolic response overcome of mice was examined. Female ICR mice (6~7 weeks of age) were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 5 groups (6 mice/cage/treatment). Mice were intubated p.o., AEY (5, 10 and 15 mg), control egg yolk (CEY, 10 mg), or fish oil (5 mg) dissolved in 0.2mL phosphate buffered saline (PBS) every two days for 14 days. At day 15, the 0.1 mL of lipopolysaccharide solution (LPS, 30 μg/0.1 mL 10 mM HEPES) was injected through tail vein, and then, the body weight of mouse and the amount of feed intake were measured over a period of 72 hours. Control group mice were received only PBS and LPS. AEY treatment suppressed the loss of mice body weight in a dose-response manner. Twenty four hours post LPS injection, the reduced body weight per mouse of AEY 5, AEY 10, and AEY 15 mg treatment groups was 3.70, 3.54, and 3.25 g, respectively. Body weight suppression effect of AEY treatment was greater than that of CEY, but less than fish oil. AEY treatment did not alter thymus weight, but increased the weight of spleen or liver. These results indicate that AEY suppressed the loss of body weight by LPS via any function of the spleen and/or liver.
Astaxanthin 처리 산란계로부터 생산된 난황이 Mouse Liver Microsome의 산화에 미치는 영향
김홍출(Hong Chul Kim),박숙자(Sook Jahr Park),김정곤(Jung Kon Kim),박철우(Cherl Woo Park),조용운(Yong Un Cho),조현종(Hyeon Jong Cho),하영래(Yeong Lae Ha) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.1
AEY의 acetone추출물이 mouse liver microsome의 산화에 미치는 영향을 조사하였다. Mouse liver microsome에 함유된 단백질 함량은 AEY 처리구가 CEY 처리구에 비해 상대적으로 7~48%정도가 증가하였다. 지방산 분석 결과, CEY 처리에 비해 AEY 처리구에서 stearic acid를 포함하는 포화지방산의 함량비가 상대적으로 높았으며, oleic acid, linoleic acid 등을 함유하는 불포화지방산의 함량비가 현저히 감소하였다. AEY 처리구는 Fe2+가 관여하는 Asc/Fe^(+2), NADPH/Fe^(+2)를 산화유도물질로 사용한 경우에 TC와 비슷하거나 보다 강력한 항산화 효과를 보였다. ABIN이나 CuOOH가 산화유도물질로 작용할 시에는 AEY처리에 의해 유의성있는 항산화 효과가 나타나지 않았다. Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on the oxidation of mouse liver microsome was investigated using female ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 7 treatment groups (10 mice/cage/treatment). Mice were intubated p.o., AEY (50, 100 and 150 mg) or control egg yolk (CEY, 150 mg) every week for 3 weeks. BHT (5 mg) and α-tocopherol (50 mg) were fed to mice as positive control. At week 4, the liver microsome was prepared from sacrificed mice. Protein content of mouse liver microsome with AEY treatment was relatively higher than that with CEY treatment. AEY treatment remarkably lowered the content of unsaturated fatty acids including oleic acid and linoleic acid, but raised that of the saturated fatty acids including stearic acid. AEY group showed relatively higher antioxidative activity than CEY, when used Asc/Fe^(+2) or NADPH/Fe^(+2) as oxidant. Antioxidative activity of AEY was more effective than α-tocopherol, but less effective than BHT.
Astaxanthin처리 산란계로부터 생산된 난황이 Mouse의 마크로파지 활성과 응집소가 및 용혈소가에 미치는 영향
김홍출(Hong-Chul Kim),박숙자(Sook-Jahr Park),김정곤(Jeong-Kon Kim),김영림(Young-Rim Kim),박원석(Won-Seck Park),조용운(Yong-Un Cho),조현종(Hyeon-Jong Cho),김정환(Jeong-Hwan Kim),하영래(Yeong-Lae Ha) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.6
마크로파지의 활성을 조사하기 위해 carbon clearance time을 조사한 결과 AEY 처리구가 control이나 CEY 처리구에 비해 짧았다. 특히 AEY 500 μg 처리에서 carbon clearance 시간이 5분으로 control에서 9.42분, CEY(250 μg 처리)에서 9.01분보다 유의성있는 감소를 보였다. AEY 처리에 의해 응집소가와 용혈소가가 다소 증가되었다. SRBC 처리 1일째와 3일째에 250 μg 처리구를 비교해 보면, control, CEY, AEY의 응집소가는 각각 5.50, 5.63, 6.00 및 5.25, 5.38, 5.50로 용혈소가는 각각 4.75, 5.38, 5.50 및 4.25, 5.63, 5.63으로 AEY 처리구가 control, CEY 처리구에 비해 면역 활성이 있었지만 유의성은 없었다. Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on mouse humoral immunity was investigated using male ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 5 treatment groups (9 mice/cage/treatment). Mice were intubated p.o., AEY (100, 250 and 500 μg) or control egg yolks (CEY, 250 μg), dissolved in 0.1 mL DMSO, for consecutive 4 days. At day 5, carbon suspension (pilot drawing ink 3 mL+3% gelatine 3 mL) was injected 3 μL per 1 g body weight through tail vein. Carbon clearance time was measured at 5 and 35 minutes post the injection of carbon suspension. Another two experiments were conducted to determine the hemagglutinin-titer (HGT) and hemolysin-titer (HLT) with male ICR mouse (8 mice/cage/treatment). Mice treated with AEY were induced immune activity with SRBC. HGT and HLT were measured from the blood at day 1 and 3 after treatment of SRBC. AEY treatment reduced the carbon clearance time. Especially the carbon clearance time by 500 μg AEY treatment was 5.00 minutes, which was very short time compared with 9.42 minutes by control and 9.01 minutes by CEY. AEY group showed slightly higher values of HGT and HLT than CEY group and control. At day 1, HGT in control, 250 μg CEY and 250 μg AEY groups was 5.50, 5.63, and 6.00, respectively. Similarly, HLT in control, 250 μg CEY and 250 μg AEY groups was 4.75, 5.38, and 5.50, respectively, at day 1. These results suggest that AEY exhibited immunity-enhancing effect.