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Conjugated Linoleic Acid (CLA) Glycerol 유도체의 화학적 합성
박원석(Won-Seck Park),김석종(Seck-Jong Kim),박숙자(Sook-Jahr Park),김정옥(Jeong-Ok Kim),임동길(Dong-Gil Lim),하영래(Yeong-Lae Ha) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.3
CLA의 glycerol 유도체를 화학적으로 합성하였다. CLA-Cl(1.79 mmole), glycerol(0.6 mmole)과 pyridine(1.3 mL)를 25C에서 8시간 반응시켰다. 이 반응물을 SGCC와 TLC를 이용하여 CLA의 glycerol 유도체를 분리하고, ¹H-NMR, ¹³C-NMR, IR, MS를 이용하여 분리된 유도체를 동정하였다. 이 실험조건하에서 사용된 CLA의 59.4%가 CLA의 glycerol 유도체(CLA-TG, CLA-DG, CLAMG)로 전환되었다. CLA의 glycerol 유도체 중 CLA-TG는 52.1%, CLA-DG는 17.0%, CLA-MG는 30.9%였다. Conjugated linoleic a cid (CLA) is a potent anticarcinogen for several animal models. CLA was synthesized by alkaline isomerization of linoleic acid. Derivatives of CLA with glycerol were synthesized by chemical methods to use as food additives. Chemically-synthesized CLA-chloride (CLA-Cl, 1.79 mmole), glycerol (0.6 mmole) and pyridine (1.3 ml) were reacted at 25℃ for 8 hrs. The resultant was fractionated by silica gel column chromatography (SGCC) and thin layer chromatography (TLC). The fractions were identified using infrared spectroscopy (IR), nuclear magnetic resonance spectroscopy (NMR), and mass spectrometry (MS). Amount of CLA converted to CLA-glycerol derivatives was 59.4% and the rest of CLA was remained as unreacted CLA or CLA dimer. The composition of the CLA-glycerol derivatives was 52.1% tri-CLA- glycerol (CLA-TG), 17.0% di-CLA-glycerol (CLA-DG) and 30.9% mono-CLA-glycerol (CLA-MG). These results suggest that the chemical synthesis of CLA-glycerol derivatives produces CLA-TG, CLA- DG and CLA-MG as well as CLA dimer.
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Astaxanthin처리 산란계로부터 생산된 난황이 Mouse의 마크로파지 활성과 응집소가 및 용혈소가에 미치는 영향
김홍출(Hong-Chul Kim),박숙자(Sook-Jahr Park),김정곤(Jeong-Kon Kim),김영림(Young-Rim Kim),박원석(Won-Seck Park),조용운(Yong-Un Cho),조현종(Hyeon-Jong Cho),김정환(Jeong-Hwan Kim),하영래(Yeong-Lae Ha) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.6
마크로파지의 활성을 조사하기 위해 carbon clearance time을 조사한 결과 AEY 처리구가 control이나 CEY 처리구에 비해 짧았다. 특히 AEY 500 μg 처리에서 carbon clearance 시간이 5분으로 control에서 9.42분, CEY(250 μg 처리)에서 9.01분보다 유의성있는 감소를 보였다. AEY 처리에 의해 응집소가와 용혈소가가 다소 증가되었다. SRBC 처리 1일째와 3일째에 250 μg 처리구를 비교해 보면, control, CEY, AEY의 응집소가는 각각 5.50, 5.63, 6.00 및 5.25, 5.38, 5.50로 용혈소가는 각각 4.75, 5.38, 5.50 및 4.25, 5.63, 5.63으로 AEY 처리구가 control, CEY 처리구에 비해 면역 활성이 있었지만 유의성은 없었다. Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on mouse humoral immunity was investigated using male ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 5 treatment groups (9 mice/cage/treatment). Mice were intubated p.o., AEY (100, 250 and 500 μg) or control egg yolks (CEY, 250 μg), dissolved in 0.1 mL DMSO, for consecutive 4 days. At day 5, carbon suspension (pilot drawing ink 3 mL+3% gelatine 3 mL) was injected 3 μL per 1 g body weight through tail vein. Carbon clearance time was measured at 5 and 35 minutes post the injection of carbon suspension. Another two experiments were conducted to determine the hemagglutinin-titer (HGT) and hemolysin-titer (HLT) with male ICR mouse (8 mice/cage/treatment). Mice treated with AEY were induced immune activity with SRBC. HGT and HLT were measured from the blood at day 1 and 3 after treatment of SRBC. AEY treatment reduced the carbon clearance time. Especially the carbon clearance time by 500 μg AEY treatment was 5.00 minutes, which was very short time compared with 9.42 minutes by control and 9.01 minutes by CEY. AEY group showed slightly higher values of HGT and HLT than CEY group and control. At day 1, HGT in control, 250 μg CEY and 250 μg AEY groups was 5.50, 5.63, and 6.00, respectively. Similarly, HLT in control, 250 μg CEY and 250 μg AEY groups was 4.75, 5.38, and 5.50, respectively, at day 1. These results suggest that AEY exhibited immunity-enhancing effect.
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