한국인 모유와 조제분유 중의 공액리놀레산

박철우,반경녀,이영남,김정옥,김민석,하영래,Park, Cherl-Woo,Bahn, Kyeong-Nyeo,Lee, Young-Nam,Kim, Jeong-Ok,Kim, Min-Seok,Ha, Yeong-Lae 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.3

The concentrations of conjugated linoleic acid (CLA) in twelve Korean mothers' milk samples collected in October 2006 and four infant formula samples produced by Korean manufacturers were determined. CLA detected from both mothers' milk and infant formula samples was only cis-9, trans-11 CLA isomer. The amount of cis-9, trans-11 CLA in Korean mothers' milk (100 mL) ranged from 4.32 to 10.12 mg ($6.27{\pm}0.28\;mg$). Infant formula (13 g), which is the manufacturers' recommended amount to make up 100 mL with water, contained cis-9, trans-11 CLA isomer in amounts ranging from 1.61 to 2.97 mg ($2.27{\pm}0.31\;mg$). 한국인 모유에는 cis-9, trans-11 CLA 이성체만 동정되었고, 그 함량은 $4.32{\sim}10.12\;mg/100\;mL$(평균 $6.27{\pm}0.28\;mg/100\;mL$)이었고, 지방함량 중에는 $1.35{\sim}3.13\;mg/g$(평균 $1.96{\pm}0.06\;mg/g$)으로 개인에 따라 큰 차이가 있었다. 한국인 모유에 함유된 CLA의 함량은 외국의 CLA 함량에 비해 낮았고, 그 이유는 식사의 차이 때문인 것으로 추정되었다. 국내에서 제조된 조제분유에서도 cis-9, trans-11 CLA만 동정되었는데, 그 함량은 $2.35{\sim}22.82\;mg/100\;g$으로 평균치는 $17.45{\pm}1.24\;mg/100\;g$이었고, 지방에 함유된 cis-9, trans-11 CLA 함량은 $0.45\;mg/g{\sim}0.85\;mg/g$으로 그 평균치는 $0.65{\pm}0.02\;mg/g$이었다. 영유아가 조제분유 제조사의 지시된 13% 조제분유를 섭취할 경우 cis-9, trans-11 CLA를 2.27 mg 섭취하는 것으로 모유 100 mL에 함유되어 있는 양에 비해 낮다. 따라서 조제분유를 조제할 때 모유와 동일한 cis-9, trans-11 CLA를 섭취할 수 있도록 조제분유를 제조하는 것이 바람직하다.

Sarcoma - 180 Cell로 유발한 Mouse 복수암에 대한 Astaxanthin 함유 난황의 효과

이상호(Sang-Ho Lee),박철우(Cherl-Woo Park),박경아(Kyung-Ah Park),이영춘(Young-Choon Lee),최의성(Eui-Sung Choi),하영래(Yeong Lae Ha) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.1

몸무게당 25㎍ AEY를 처리한 mouse는 24.8일의 수명을 보여 대조구에 비하여 약 38%의 수명이 연장되었으며, 10㎍ AEY를 처리한 mouse는 19.4일, 50㎍ AEY를 처리한 mouse는 21.9일간 생존하여 각각 8%, 22%의 수명 연장효과를 볼 수 있었으나, CEY 50㎍을 처리한 mouse는 4%의 수명이 연장되었다. 따라서 AEY의 복수암에 대한 수명연장효과는 25㎍ AEY의 처리농도에서 가장 큰 효과를 볼 수 있었다. Anticarcinogenic activity of astaxanthin-containing egg yolk(designate AEY) was investigated for mouse ascites carcinogenesis induced by mouse Sarcoma-180(S-180) cells. Female ICR mice(8 mice/treatment, 7~8 weeks of age, 25±1g) were injected, i.p. with S-180 cells(1×10^7cell/ml PBS). Two days later, each mouse was given 0.1ml PBS containing AEY(10, 25 or 50㎍/g body weight) or control egg yolk(CEY ; 50㎍/g body weight) every other day for 7 times. Control mice were only given 0.1ml S-180 cells and 0.1ml PBS. Mice treated with 25㎍/g body weight of AEY showed 24.8 days of life, which was equivalent to 138% of control mice's life(18.0 days). Based on dose-dependant experiment of AEY, mice treated with 10㎍/g body weight showed slightly longer life(19.4 days) relative to mice treated with control mice, and mice treated with 50㎍/g body weight exhibited 21.9 days of life. Mice treated with any dose of AEY exhibited longer life than mice with CEY 50㎍/g body weight. Body weight of mice treated with AEY was reduced relative to that of control mice or CEY-treated mice. These results suggest that AEY inhibits the carcinogenesis of mouse ascites induced by S-180 cells.

난소절제 흰쥐에서 신령버섯의 β-Glucan과 난각 Ca 복합체가 골 대사에 미치는 효과

노경희,장지현,김진주,박철우,김정옥,김재철,송영선,Noh, Kyung-Hee,Jang, Ji-Hyun,Kim, Jin-Ju,Park, Cherl-Woo,Kim, Jeong-Ok,Kim, Jae-Cherl,Song, Young-Sun 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.10

본 연구는 칼슘 급원으로서 $\beta-glucan-egg$ shell calcium complex($\beta-glucan-Ca$ 복합체)의 유효성을 확인하여 칼슘 보충제 개발에 활용하고자 실시하였다. 신령버섯에서 추출한 $\beta-glucan$과 난각의 Ca을 결합시켜 $\beta-glucan-Ca$ 복합체로 제조하여 골다공증 실험동물에서 $\beta-glucan$ 및 $\beta-glucan-Ca$ 복합체가 골 대사에 미치는 효과를 비교하였다. 10주령의 Sprague-Dewley 암컷 흰쥐의 양쪽 난소를 절제(OVX)한 후 실험식 이를 급여하였으며 Sham-대조군(Sham-C)은 실험군과 동일한 스트레스를 주기 위해 난소를 절제하지 않고 개복수술만 실시한 후 실험하였다. 식이조성은 Sham-C, OVX-대조군(OVX-C)와 $OVX-\beta-glucan$군(OVX-G)은 $CaCO_3$로 Ca 함량이 0.5%가 되도록 조정하였으며, OYX-G군은 $\beta-glucan$을 첨가하여 제공하였다. $OVX-\beta-glucan-Ca$ 복합체군(OVX-GE)은 $\beta-glucan-Ca$ 복합체의 Ca 농도가 0.5% 함유하도록 조정하여 실험식이를 각각 조제하여 6주간 급여하였다. 체중 100 g당 건조시킨 대퇴골의 무게는 Sham-C>OVX-GE>OVX-C와 OVX-G의 순으로 나타나 $\beta-glucan-Ca$ 복합체를 제공한 OVX-GE에서 다른 OVX군에 비해 상대적으로 골 무게가 증가하는 경향을 보였다. 대퇴골의 골밀도는 각 군 간의 유의적인 차이를 보였으며 Sham-C에 비해 $\beta-glucan$을 첨가한 식이를 제공받은 군에서 골밀도가 상대적으로 높은 수준을 보였고 특히 OVX-GE에서 가장 높은 골밀도를 보였다. Ca체내 흡수율과 보유량은 각 군 간에 유의적인 차이를 보였으며 OVX-C가 Sham-C에 비해 높았으며 OVX군에서는 $\beta-glucan$를 첨가한 군이 높은 수준을 보였다. ALP의 활성은 OVX군에서는 $\beta-glucan$을 첨가한 군이 다소 낮은 경향을 보였다. 요 중의 골 흡수 지표인 DPD crosslink value는 각 군 간의 유의적인 차이를 보였고 OVX군의 DPD crosslink value가 Sham-C에 비해 현저하게 높은 수준으로 나타나 OVX군의 골 흡수가 높음을 확인할 수 있었다. OVX군에서 $\beta-glucan$을 첨가한 식이를 제공한 OVX-G와 OYX-GE의 DPD crosslink value가 OVX-C에 비해 유의적으로 낮은 수준이었으며 $\beta-glucan$이 난소 절제 흰쥐 모델에서 골 대사에 영향을 미쳐 골 흡수율을 낮추어 골 보호효과를 나타내는 것으로 보인다. 이상의 결과들로 미루어 볼 때, 비록 $\beta-glucan$을 제공한 군과 $\beta-glucan-Ca$ 복합체를 제공한 두 군 간에는 유의적인 차이를 보이지 않았으나 난소절제 흰쥐모델의 골 대사에서 $\beta-glucan-Ca$복합체 형태가 골다공증 예방을 위한 기능성 식품으로서의 가능성을 보여주었으며, 앞으로 골다공증 예방을 위한 $\beta-glucan-Ca$ 복합체 형태의 효과적인 농도는 계속적으로 연구가 진행되어야 할 것으로 사료된다. This study was designed to evaluate the effect of Agaricus blazei $\beta-glucan$ and egg shell calcium complex on bone metabolism in ovariectomized (OVX) rats. Forty Sprague-Dewley female rats, 10 weeks of age $(248{\pm}1.7g)$, were divided into 4 groups and fed on the experimental diets for 6 weeks: sham operated control treated with normal diet containing 0.5% calcium (Sham-C), OVX-control treated with normal diet containing 0.5% calcium (OVX-C), $OVX-\beta-glucan$ group treated with $\beta-glucan$ diet containing 0.5% calcium (OVX-G), and $OVX-\beta-glucan$ egg shell calcium complex treated with $OVX-\beta-glucan$ egg shell calcium complex containing 0.5% calcium (OVX-GE). Bone weight of femur was higher in the OVX-GE group than in the other OVX groups. Bone mineral density of femur was significantly different (p<0.05) among the experimental groups and showed the highest level in the OVX-GE group. Calcium absorption rate and retention were higher in the $\beta-glucan$ supplement groups than in the other groups (p<0.05). Alkaline phosphatase activities and osteocalcin levels of serum showed lower in the $\beta-glucan$ supplement groups than in the OVX-C group. Deoxypyridinoline crosslink values of urine, indicator of bone absorption, showed the lowest in the OVX-GE group. The $\beta-glucan$ supplemented groups had a lower bone resorption ratio than in the OVX-C group. We concluded that bioavailability of calcium is higher in $\beta-glucan$ supplement groups compared to those in OVX rats. From the above results, these findings suggest the possibility of using $\beta-glucan$ egg shell calcium complex as a functional food material related to bone metabolism, even though there is no significant difference between the groups of $\beta-glucan$ and $\beta-glucan-egg$ shell calcium complex supplementation.

항암성과 향미가 개선된 재래식 버섯균사체메주의 제조

김영숙(Young-Suk Kim),박철우(Cherl-Woo Park),김석종(Seck-Jong Kim),박숙자(Sook-Jahr Park),류충호(Chung-Ho Ryu),조현종(Hyun-Jong Cho),김정옥(Jeong-Ok Kim),임동길(Dong-Kil Lim),하영래(Yeong-Lae Ha) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.6

항암성과 향미가 개선된 기능성 재래식 버섯균사체메주는 버섯균을 액체배양하여 재래식메주의 각 면에 5개씩 만든 구멍(1×3 cm)에 버섯균배양액을 메주 무게의 10%를 접종한 후 25℃에서 4주간 배양하여 제조하였다. 제조한 버섯균사체 메주 중 상황, 영지, 또는 신령버섯균사체메주가 항암성과 향미완화능이 우수하였다. Mushroom mycelia-cultured traditional meju (MTM) was prepared by inoculating 10% submerged-liquid culture of mushroom strains to five holes (1×3 cm) per side of the traditionally-fermented meju (10×10×10 cm), followed by incubating additional 4 weeks at 25℃. Mushroom strains used were Neutari (Pleurotus ostreatus, PO), Yeongji (Ganoderma lucidum, GL), Synryeong (Agaricus blazei, AB), Ypsae (Grifola frondosa, GF), Pyogo (Lentinus edodes, PE), Dongchunghacho (Paecilomyces japonicus, PJ) and Sanghwang (Phellinus linteus, PL). All MTMs showed an enhanced anticarcinogenicity against S-180 cell-induced mouse ascites cancer, antimutagenicity against aflatoxin B₁ (AFB₁) and 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), and sensory qualities, relative to control meju. Such positive effects of MTM prepared with Sanghwang, Yeongji, or Synryeong were superior to those of MTM with Ypsae, Pyogo, Dongchunghacho, or Neutari.

재래식 버섯균사체 된장의 항종양성과 항돌연변이성

김석종(Seck-Jong Kim),박철우(Cherl-Woo Park),박숙자(Sook-Jahr Park),김영숙(Young-Suk Kim),조현종(Hyun-Jong Cho),임동길(Dong-Kil Lim),김정옥(Jeong-Ok Kim),이주희(Joo-Hee Lee),하영래(Yeong-Lae Ha) 한국식품영양과학회 2003 한국식품영양과학회지 Vol.32 No.1

재래식 버섯균사체메주로부터 제조된 재래식 버섯균사체 된장의 항종양성, 항산화성 및 항돌연변이성을 연구하였다. 항종양성(S-180 세포로 유발한 mouse ascites cancer)에서는 느타리, 영지, 상황, 신령버섯균사체된장이 각각 33, 30, 26, 25% 억제하여 재래식 된장(11%)에 비해 매우 우수한 항종 양성을 나타내었다. 버섯균사체된장은 S. typhimurium TA 98에 대한 IQ와 AFB1에 의한 돌연변이성을 각각 39.7~46.1%, 38.0~39.7% 억제하여 대조된장(32.0, 33.2%)에 비해 항돌연변이성이 우수하였다. S. typhimurium TA 100에 대해서는 IQ와 AFB1의 돌연변이성을 각각 31.9~35.6%, 24.9~30.4% 억제하여 대조된장(27.5, 22.5%)에 비해 항돌연변이성이 좋았다. 항산화성 역시 버섯균사체된장이 대조된장에 비해 효과가 우수하였다. 관련 물질로서 β-D-glucan 함량은 버섯균사체된장의 경우 재래식 된장에 비하여 3~8배, 총 phenol성 화합물과 isoflavone 함량은 느타리버섯균사체된장과 신령버섯균사체가 가장 높았다. Antitumorigenic and antimutagenic activities of the doenjangs prepared from mushroom mycelia-cultured traditional mejus (designated to MTDJ) were investigated using the model of Sarcoma-180-induced mouse ascites cancer, and 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) and aflatoxin B1 (AFB1)-mediated S. typhimurium mutagenicity, respectively. Antioxidative activity of MTDJ was also investigated using the mouse liver microsome system. Mushroom stains used for the preparation of the mushroom mycelia-cultured traditional mejus were Synryeong (Agaricus blazei), Yeonggi (Ganoderma lucidum), Sanghwang (Phellinus linteus), and Neutari (Pleurotus ostreatus). All MTDJs showed the enhanced antitumorigenicities (12% by Synryeong, 13% by Sanghwang, 16% by Yeonggi, and 19% by Neutari), antimutagenicity (6.1~20.8% for IQ and 3.1~10.2% for AFB1), and antioxidative activity (6.6~46.5%), relative to the control doenjang. The β-D-glucan content (0.75~1.71 mg/g) of MTDJs was 3~8 times higher than that (0.22 mg/g) of the control doenjang. Genistein content (769~932 μg/g) of MTDJs was also higher than that (728 μg/g) of control doenjang. The content of β-D-glucan and genistein was not exactly correlated to the antitumorigenicity and antimutagenicity of MTDJs. These results indicate that antitumorigenicity and antimutagenicity of MTDJs were elevated in comparison with the control doenjang, and the observed functions were, in part, derived from β-glucan and/or genistein in the MTDJs.

Astaxanthin 처리 산란계로부터 생산된 난황이 Mouse Liver Microsome의 산화에 미치는 영향

김홍출(Hong Chul Kim),박숙자(Sook Jahr Park),김정곤(Jung Kon Kim),박철우(Cherl Woo Park),조용운(Yong Un Cho),조현종(Hyeon Jong Cho),하영래(Yeong Lae Ha) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.1

AEY의 acetone추출물이 mouse liver microsome의 산화에 미치는 영향을 조사하였다. Mouse liver microsome에 함유된 단백질 함량은 AEY 처리구가 CEY 처리구에 비해 상대적으로 7~48%정도가 증가하였다. 지방산 분석 결과, CEY 처리에 비해 AEY 처리구에서 stearic acid를 포함하는 포화지방산의 함량비가 상대적으로 높았으며, oleic acid, linoleic acid 등을 함유하는 불포화지방산의 함량비가 현저히 감소하였다. AEY 처리구는 Fe2+가 관여하는 Asc/Fe^(+2), NADPH/Fe^(+2)를 산화유도물질로 사용한 경우에 TC와 비슷하거나 보다 강력한 항산화 효과를 보였다. ABIN이나 CuOOH가 산화유도물질로 작용할 시에는 AEY처리에 의해 유의성있는 항산화 효과가 나타나지 않았다. Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on the oxidation of mouse liver microsome was investigated using female ICR mouse (6~7 weeks of age). Mice were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 7 treatment groups (10 mice/cage/treatment). Mice were intubated p.o., AEY (50, 100 and 150 mg) or control egg yolk (CEY, 150 mg) every week for 3 weeks. BHT (5 mg) and α-tocopherol (50 mg) were fed to mice as positive control. At week 4, the liver microsome was prepared from sacrificed mice. Protein content of mouse liver microsome with AEY treatment was relatively higher than that with CEY treatment. AEY treatment remarkably lowered the content of unsaturated fatty acids including oleic acid and linoleic acid, but raised that of the saturated fatty acids including stearic acid. AEY group showed relatively higher antioxidative activity than CEY, when used Asc/Fe^(+2) or NADPH/Fe^(+2) as oxidant. Antioxidative activity of AEY was more effective than α-tocopherol, but less effective than BHT.

Astaxanthin 처리 산란계로부터 생산된 난황의 Mouse에 대한 Catabolic Response Overcome 효과

김홍출(Hong-Chul Kim),박숙자(Sook-Jahr Park),박철우(Cherl-Woo Park),김영림(Young-Rim Kim),김정환(Jeong-Hwan Kim),최의성(Eui Sung Choi),조현종(Hyeon-Jong Cho),조용운(Yong Un Cho),하영래(Yeong Lae Ha) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.6

Astaxanthin을 산란계에 경구 투여하여 얻은 AEY에는 총 2.88 mg%의 carotenoid가 함유되어 있었다. Carotenoid 성분을 HPLC로 분석한 결과, CEY에는 존재하지 않는 α-cryptoxanthin, canthaxanthin, cynthiaxanthin, triol 등이 AEY에서 확인되었다. CEY에서는 존재하지 않기 때문에 이들은 astaxanthin의 대사생성물로 예상할 수 있다. AEY는 LPS로 유발한 mouse의 catabolic response overcome의 효과 시험에서 control구에 비해 유의성있는 체중 감소 억제 효과를 보였다. 또한 AEY는 LPS 처리에 의한 면역기관에 미치는 영향에서는 췌장과 간의 체중에 대한 상대적 비율을 증가시켰다. 따라서 AEY 처리가 면역작용을 하는 세포의 활성에 관여하는 것으로 생각되고 이것은 AEY 처리구에서 관찰되는 α-cryptoxanthin, canthaxanthin, cynthiaxanthin, triol 등에 의한 것으로 추측된다. Effect of the egg yolks from laying hens intubated, p.o., astaxanthin (designated AEY) on the catabolic response overcome of mice was examined. Female ICR mice (6~7 weeks of age) were adapted in a temperature- and humidity-controlled house for one week and randomly divided into 5 groups (6 mice/cage/treatment). Mice were intubated p.o., AEY (5, 10 and 15 mg), control egg yolk (CEY, 10 mg), or fish oil (5 mg) dissolved in 0.2mL phosphate buffered saline (PBS) every two days for 14 days. At day 15, the 0.1 mL of lipopolysaccharide solution (LPS, 30 μg/0.1 mL 10 mM HEPES) was injected through tail vein, and then, the body weight of mouse and the amount of feed intake were measured over a period of 72 hours. Control group mice were received only PBS and LPS. AEY treatment suppressed the loss of mice body weight in a dose-response manner. Twenty four hours post LPS injection, the reduced body weight per mouse of AEY 5, AEY 10, and AEY 15 mg treatment groups was 3.70, 3.54, and 3.25 g, respectively. Body weight suppression effect of AEY treatment was greater than that of CEY, but less than fish oil. AEY treatment did not alter thymus weight, but increased the weight of spleen or liver. These results indicate that AEY suppressed the loss of body weight by LPS via any function of the spleen and/or liver.

Reduction of Visceral and Body Fats in Mice by Supplementation of Conjugated Linoleic Acid with γ-Oryzanol

Jae Il Byeon(변재일),Tae Woo Ohr(오태우),Young Suk Kim(김영숙),Yeon Gyu Moon(문연규),Cherl Woo Park(박철우),Jeong Ok Kim(김정옥),Yeong Lae Ha(하영래) 한국생명과학회 2008 생명과학회지 Vol.18 No.9

Conjugated linoleic acid (CLA)와 γ-oryzanol (OZ) 혼합물의 mouse 체지방 및 복부지방 감소에 관한 연구를 하였다. Female ICR mice (10주 령)을 몸무게 차이가 없도록 Control (70 μl olive oil + 30 μl CLA), CLA-OZ 1 (70 μl olive oil + 30 μl CLA + 0.5 ㎎ OZ), CLA-OZ 2 (70 μl olive oil + 30 μl CLA + 1.0 ㎎ OZ), OZ (100 μl olive oil + 1.0 ㎎ OZ) 및 Olive oil (100 μl olive oil)로 구분하였다. 처리시료는 매일 4주간 경구투여 하였고 식이와 물은 자유롭게 먹게 하였다. 시료처리 4주 후에 mice의 몸무게를 달고 탈골법으로 sacrifice하여 전체몸무게, 복부지방 무게, 장기와 복부지방을 제거한 나머지 부분 무게 (empty carcass weight: ECW)를 측정하였다. CLA (control) 처리는 olive oil 처리에 비해 유의성 있게 몸무게, ECW, 복부지방 및 체지방을 감소시켜 CLA가 복부지방 및 체지방을 감소시킴을 알 수 있었다. CLA-OZ 1 처리는 OZ 처리에 비해서는 복부지방 및 체지방을 유의성 (p<0.05) 있게 감소시켰으나 CLA (control) 처리에 비해서는 유의성이 없었다. 또한 CLA-OZ 2 처리는 control 처리 및 OZ 처리에 비해 복부지방 및 체지방을 유의성 (p<0.05) 있게 감소시켰다. 이 결과는 CLA와 OZ를 혼합하여 처리한 mice에서 이들을 각각 단독으로 처리한 mice에서보다 복부지방 및 체지방이 많이 감소되어 OZ가 CLA의 지방감소 작용에 상승효과가 있음을 의미한다. The synergistic effect of conjugated linoleic acid (CLA) and γ-oryzanol (OZ) on the reduction of visceral and body fats was investigated in mice. Female ICR mice, 10 weeks of age, were acclimated for one week and then randomly divided into 5 treatment groups by body weights: Control (70 μl olive oil + 30 μ CLA), CLA-OZ 1 (70 μl olive oil + 30 μl CLA + OZ 0.5 ㎎), CLA-OZ 2 (70 μl olive oil + 30 μl CLA + OZ 1.0 ㎎), OZ (100 μl olive oil + OZ 1.0 ㎎), and Olive oil (100 μl olive oil). Samples were daily intubated, p.o., for 4 weeks. Food and water were ad libitum. Four weeks later, mice were sacrificed by neck dislocation, followed by measuring whole body weight, empty carcass weight (ECW), which is weight without organs and visceral fats, visceral fats, body fats and protein content. Mice treated with CLA (control) sample maintained significantly, p<0.05, lower whole body weight, ECW, visceral and body fats, relative to mice treated with olive oil sample, indicating that CLA reduces the visceral and body fats. The CLA-OZ 1 treatment significantly reduced, p<0.05, visceral and body fats as compared to OZ treatment, but not significantly different from control treatment. Meanwhile, CLA-OZ 2-treated mice maintained significantly, p<0.05, lower visceral and body fats than control and OZ-treated mice. Protein contents in mice were not affected by any other treatments. These results suggest that OZ enhanced the reduction of visceral and body fats in mice by CLA.

난소절제 흰쥐에서 신령버섯의 β⁃Glucan과 난각 Ca 복합체가 골 대사에 미치는 효과

노희경(Kyung Hee Noh),장지현(Ji Hyun Jang),김진주(Jin Ju Kim),박철우(Cherl Woo Park),김정옥(Jeong Ok Kim),김재철(Jae Cherl Kim),송영선(Young Sun Song) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.10

This study was designed to evaluate the effect of Agaricus blazei β-glucan and egg shell calcium complex on bone metabolism in ovariectomized (OVX) rats. Forty Sprague-Dewley female rats, 10 weeks of age (248±1.7 g), were divided into 4 groups and fed on the experimental diets for 6 weeks: sham operated control treated with normal diet containing 0.5% calcium (Sham-C), OVX-control treated with normal diet containing 0.5% calcium (OVX-C), OVX-β-glucan group treated with β-glucan diet containing 0.5% calcium (OVX-G), and OVX-β-glucan egg shell calcium complex treated with OVX-β-glucan egg shell calcium complex containing 0.5% calcium (OVX-GE). Bone weight of femur was higher in the OVX-GE group than in the other OVX groups. Bone mineral density of femur was significantly different (p<0.05) among the experimental groups and showed the highest level in the OVX-GE group. Calcium absorption rate and retention were higher in the β-glucan supplement groups than in the other groups (p<0.05). Alkaline phosphatase activities and osteocalcin levels of serum showed lower in the β-glucan supplement groups than in the OVX-C group. Deoxypyridinoline crosslink values of urine, indicator of bone absorption, showed the lowest in the OVX-GE group. The β-glucan supplemented groups had a lower bone resorption ratio than in the OVX-C group. We concluded that bioavailability of calcium is higher in β-glucan supplement groups compared to those in OVX rats. From the above results, these findings suggest the possibility of using β-glucan egg shell calcium complex as a functional food material related to bone metabolism, even though there is no significant difference between the groups of β-glucan and β-glucan-egg shell calcium complex supplementation.

뽕나무가루 첨가 배지에서 배양한 버섯균사체 배양물의 자유라디칼 유도 산화 억제

김석종(Seck-Jong Kim),임동길(Dong-Kil Lim),박철우(Cherl-Woo Park),세르보로다메(Rhoda Mae Cerbo),형석원(Seok-Won Hyung),이강권(Kang-Kweon Lee),김정옥(Jeong-Ok Kim),하영래(Yeong-Lae Ha) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.2

뽕나무가루 첨가 배지에서 배양한 느타리버섯균사체 배양물의 조추출물이 자유라다칼로 유도한 linloeic acid의 산화를 대조구에 비해 75.9% 감소시켰고, mouse liver microsome 산화에서도 NADPH/Fe^(++) system에서 64.3% 감소시켰다. 이와 같은 효과는 조추출물로부터 용매분획한 분획물의 단독 효과보다 우수하였다. 상황 및 동충하초버섯균사체 배양물의 조추출물도 느타리버섯균사체 배양물의 조추출물과 효과가 유사하였지만 다소 낮았다. Antioxidant activity of extracts from the submerged-liquid culture of mushrooms was measured using two systems: linoleic acid and mouse liver microsomes induced by various free radical sources. Mushrooms of Pleurotus ostreatus (Neutari), Phellinus linteus (Sanghwang), Paecilomyces japonicus (Dongchunghacho), Hericicum erinacium (Norugungdengyee) and Agaricus blazei (Shinryeong) in 1% mulberry tree powder-supplemented medium were incubated in a shaking incubator (200 rpm, 25℃) for 3 days. Hot water extracts of mycelial cultures were freeze-dried, followed by fractioning with hexane, chloroform, ethylacetate, and butanol in the order. Antioxidant activity of each sample was examined in free radical-induced linoleic acid oxidation in phosphate-buffered saline (PBS) solution by measuring the amount of malonaldehyde (MA), and mouse liver microsomal systems by measuring the amount of thiobarbituric acid reactive substances (TBARS). In linoleic acid oxidation system, hot water extracts from the cultures of Pleurotus ostreatus, Phellinus linteus, and Paecilomyces japonicus exhibited stronger antioxidant activity than aqueous or butanol fraction and the combined fraction of hexane, chloroform and ethylacetate, but the hot water extract from Pleurotus ostreatus culture was the strongest activity. The antioxidant activity of the hot water extract from Pleurotus ostreatus culture was stronger than any other fractions in mouse microsomal system. These results suggest that hot water extract of Pleurotus ostreatus culture, and the cultures of Phellinus linteus and Paecilomyces japonicus could be useful for functional materials to reduce the oxidation of lipids in food systems induced by free radicals.