Algal bioassay 에 의한 조류생장 제한영양염류 결정

오희목(Hee Mock Oh),이석준(Seog June Lee),김성빈(Seong Bin Kim),박미경(Mi Kyung Park),윤병대(Byung Dae Yoon),김도한(Do Han Kim) 한국하천호수학회 1998 생태와 환경 Vol.31 No.2

Determination of Limiting Nutrient for Algal Growth by Algal Bioassay. Oh, Hee-Mock, Seog June Lee, Seong-Bin Kim, Mi- Kyung Park, Byung-Dae Yoon and Do-Han Kim (Environmental Microbiology Research Unit, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-333, Korea and Water Resources Research Institute, Korea Water Resources Corporation, Taejon 305-390, Korea) Water quaiity and algal growth potential (AGP) using Microcystis aeruginosa were investigated from April to September 1997 in Daechung Reservoir. Secchi disk transparency was 1.1 m in August. Chlorophyll-a concentrations were higher than 10㎍/l at all samples. Total phosphorus concentration was in a range of 0.020∼0.027mg/l which is at the approximate value of 0.03 mg/l, the general critical level of eutrophication. These results indicate that Daechung Reservoir is in an eutrophic state except some temporary times and restricted sites. Phosphorus uptake rate of M. aeruginosa was higher in the water from the epilimnion than in the hypolimnion and also higher under light condition than under dark condition. Therefore, phosphorus uptake rate appears to depend on cellular phosphorous content and light condition, a meaning of endergonic process. Phosphorus was the most frequently limiting nutrient: phosphorus was limiting algal gowth in April and May, nitrogen, phosphorus and trace elements were the limiting factors for algae in August, and trace elements in September. If a fast growing alga, M, aeruginosa, is used as a test organism for assaying AGP, in vivo fluorescence instead of dry weight can be measured for algal biomass and incubation period can be reduced from traditional 14 days to 7 days.

팔당호의 생태학적 수질환경의 변화와 미생물 분포

허성남,민경희,유재근,최순영 숙명여자대학교 자연과학연구소 1999 자연과학논문집 Vol.- No.10

팔당호의 생태학적 환경요인의 계절적인 변화를 분석하므로서 이것으로 인한 식물성 플랑크톤의 연관성을 고찰과 함께 오염과정을 파악하여 수질오염의 예방의 기초자료를 얻고자 본 실험을 수행하였다. 1994년 4월부터 1995년 10월까지 계절별로 3개 지점에서 시료를 채취하여 표층부와 저층부의 수질을 분석하였다 용존산소(DO)와 pH는 각각 7.0-11.6 ㎎/ℓ와 6.9-8.9의 범위이었으며 COD와 BOD농도는 각각 1.6-4.8 ㎎/ℓ와 0.8-2.9 ㎎/ℓ이었으며,SS는 1.8-8.6 ㎎/ℓ의 농도차를 보여 주었다. 또한 NH₃-N와 PO₄-P의 농도는 각각 0.161-0.478 ㎎/ℓ와 0.003-0.073 ㎎/ℓ이었다. 표층의 chlorophyll a의 농도는 낮게는 북한강 유입부위인 12.3 ㎎/ℓ로부터 가장 높은 경안천 유입부위 23.8 ㎎/ℓ의 범위를 보여주고 있다. pH,전도도, COD, BOD, SS ,총인, chlorophyll a의 농도는 하계절에 가장 높았으며 이 결과로 미루어 보아 하계절에는 하천에 오염물질이 가장 많이 유입되는 것으로 추정된다. Station별로 조사한 결과 이들의 농도는 경안천 하류인 St. 3가 남한강 하류인 St. 2이나 댐지역의 St. 4보다 높은 결과를 보여 주었으므로 경안천 상류에서 오염원이 가장 많이 유입되는 것으로 추정된다. 아울러 종속영양세균의 분포도 경안천이 다른 지역보다 가장 높게 나타났다. Seasonal variation of water quality was examined in the reservoir, lake Paldang on Han River, the inlet stations from three rivers, North Han River, South Han liver, Kyung-An stream, and the station of dam area of the lake. Water samples were collected bimonthly from surface and bottom layers at four different stations of the lake Paldang from April 1993 to August 1994. With respect to the seasonal variation, pH, conductivity, and concentrations of COD, BOD, SS, total phosphorus, and chlorophyll a were higher in summer compared with those of autumn or other seasons, while concentrations of DO, NH₃-N, and PO₄-P were increased in winter. Concentrations of COD, BOD, chlorophyll a, total nitrogen and phosphorus were the highest at the station of Kyung-An stream rather than those of other three stations. The average concentrations of chlorophyll a surface water were from 12.3 ㎎/ℓ at North Han River to 23.8 ㎎/ℓ at Kyung-An stream. Heterotrophic bacterial distribution showed higher at the station of Kyung-An steam than those of South Han River and the dam station of the lake.

한열변증에 따른 중풍 환자의 제반특성 비교

김민경,이인환,신애숙,김나희,김혜미,심소라,나병조,조승연,박성욱,정우상,문상관,박정미,고창남,조기호,김영석,배형섭,Kim, Min-kyung,Lee, ln-whan,Shin, Ae-sook,Kim, Na-hee,Kim, Hye-mi,Shim, So-ra,Na, Byung-Jo,Cho, Seung-yeon,Park, Seong-uk,Jung, Woo Sang 대한중풍순환신경학회 2010 대한중풍.순환신경학회지 Vol.11 No.1

Object : This study was conducted as part of the national project to standardize stroke diagnosis in Korean medicine. In this study, we aimed to evaluate the characteristics of acute stroke between heat pattern group and cold pattern group. Methods : We recruited stroke patients from 5 universities(Kyung-Hee University oriental medical center, Kyung-Hee University East-West Neo medical center, Kyungwon university lncheon oriental medical center, Kyungwon university Songpa oriental medical center and DongGuk university llsan oriental medical center) from April, 2007 until February, 2010. We diagnosed them and selected 463 heat pattern patients and 182 cold pattern patients. Results : We find that the risk factor of smoking, alcohol, diet(prefer to meat) are more associated with the heat pattern group. On the other hand, inflammation history in recent 3 months and diet(prefer to sea food) are more associated with the cold pattern group. RBC, Hg, Hct, TG, CK and Cl are more related to the heat pattern group. CPT, total cholesterol and HDL are more related to the cold pattern group. Tae-eum type takes high distribution of the heat pattern group, and So-eum type takes high distribution of the cold pattern group. Conclusions : This study provides evidence that the heat pattern group have more risk factor than the cold pattern group.

Neisseria gonorrhoeae 분리균의 항생제 내성 양상 분석

성원근,정경태,길준영,김석하,오희복 대한감염학회 2001 감염 Vol.33 No.5

Background : The aim of this study was to monitor trends in antimicrobial susceptibilities of Nesseria gonorrhoeae isolates, in particular, to examine the possibility of increasing prevalence of fluoroquinolone resistance in Korea and the relationship between patterns of mutations involving gyrA and parC genes and the ciprofloxacin resistance level. Methods : The antimicrobial susceptibilities of 489 gonoccocal isolates which were nationwide collected from patients visiting Health Centers were determined by NCCLS disk diffusion and agar plate dilution methods. PCR and direct DNA sequencing of the amplicons were performed to identify mutations within the quinolone resistance-determining regions of gyrA and parC genes. Results : The proportion of resistance to penicillin, tetracycline, or both remained as high as 94%. The isolates with ciprofloxacin resistance remarkably increased from 1% in 1999 to 5% in 2000 and also resistance to cefoxitine and ceftriaxone were shown to be increased. The strains resistant to spectinomycin was little reported. Four isolates with 16 ㎍/mL of MIC for ciprofloxacin all showed the same alternations of Ser-91 to Phe, Asp-95 to Gly in GyrA and Ser-87 to Arg in ParC, but ciprofloxacin-susceptible strains have little amino acid substitution. Conclusion : Considering the increasing prevalence of isolates with resistance to ciprofloxacin and ofloxacin, it is likely that the antibiotics such as spectinomycin, or ceftriaxone are recommended as the first-line treatment for gonoccocal infections in Korea. The results from this study suggest that mutation analysis for quinolone resistance-determining regions of gyrA and parC genes are important in epidemiological studies for the spread of ciprofloxacin resistant strains.(Korean J Infect Dis 33:338∼345, 2001)

소아에서 나타난 비구개관낭의 치험례

모정희,정현구,조태식,김효석,박성규,이난영 조선대학교 구강생물학연구소 2002 口腔生物學硏究 Vol.26 No.2

The nasopalatine duct cyst is the most common non-odontogenic cyst in the oral area and is thought to be originated from the epithelial remnants of the nasopalatine duct. The nasopalatine duct cyst have been mentioned to be an overall incidence of 1.7-11.9% of all jaw cyst. More males than females are affected by that. It could occur all around the age, and especially in their 40th and 60th it happens the most and in childhood it is very rare. Proper treatment for nasopalatine duct cyst is enucleation. In the case of very large cysts, it is likely to be a risk of naso-oral or antral-oral fistulas or of devitalization of teeth, marsupialization is indicated. The purpose of case report is that a nasopalatine duct cyst in is very rare in childhood. A 9-year-old boy was referred to the chosun university pedodontics clinic that this child who has anterior palatal swelling is in routine check. The size of the cyst was so big that the marsupialization was committed and obturator was put. This patient is on the continuous observation. we treated the nasopalatine duct cyst with obturator.

BLDC 모터를 이용한 PV 양수 펌프 시스템

최성호,조정민,전기영,김성남,성낙규,김용주,한경희 명지대학교 산업기술연구소 2001 産業技術硏究所論文集 Vol.20 No.-

In this paper, we adapted BLDC motor to PV water pumping systems to maintain high efficiency in the wide speed area. Also to design confidence, we adapted the vector control that drive the maximum torque at each speed limit. We designed optimal gain value of current, speed and pressure PI controller. Inverter gate pulse used Space Vector PWM to reduce torque pulsation of BLDC motor. Finally it was improve general matters of high water storage tank method by direct water supply pumping method.

레니텍^(�) 정(말레인산 에날라프릴, 10mg)에 대한 에나레이스 정의 생물학적 동등성

조성희,하용화,홍성제,서성훈,류재환,김동현,이경태 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

The purpose of present study was to evaluate the bioequivalence of two enalapril maleate tablets, Renitec^(™) (MSD Korea Ltd.) and Enalace ^(™) (Welfide Korea Ltd.), according to the guidelines of Korea Food Drug Administration (KFDA). Twenty-four normal male volunteers, 22.33±2.55 year in age and 66.54±8.30 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After two tablets containint 10 ㎎ of enal-april maleate per tablet wre orally administered, blood was taken at predetermined time intervals and concentrations of enalapril in plasma were determined using LC-MS-MS. Pharmacokinetic parameters such as AUC_(t), C_(max) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t) and C_(max) untransformed T_(max). There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transfored data were acceptance range of log0.8 to log1.25(e.g., log1.02∼log1.14 and log1.03∼log1.19 for AUC_(t) and C_(max), respectively). The major parameters, AUC_(t) and C_(max), met the criteria of KDFA for bioequivalence indicating that Enalace^(™) tablet is bioequivalent to Renitec^(™) tablet.

가와사끼 질환에서 감마글로불린의 대량 정주 용법의 효과에 관한 연구

이경희,하태선,박범수,한헌석,하성훈 충북대학교 의과대학 충북대학교 의학연구소 1994 忠北醫大學術誌 Vol.4 No.1

가와사끼병으로 진단받고 고용량의 감마글로불린(2 gm/㎏)을 정맥주사 받은 17명의 환아를 대상으로 임상적 효과와 관상동맥의 변화에 미치는 영향을 연구하여 다음의 결과를 얻었다. 1. 대상 환아의 연령은 평균 2.03세로 3개월에서 5세 사이에 분포하였고, 2세 이하가 58.8%를 차지하고 남여의 비는 1.125 : 1이었다. 2. 가와사끼 진단 기준의 6가지 주증상 중 5가지는 전환아에서 나타났으며, 경부 림프선 종창만 64.7%에서 나타났다. 발생 계절은 봄과 겨울에 76.5%의 환아가 발생하였다. 3. 대량의 감마글로불린 주사 직후 평균 11.4시간에 고열이 없어지고 다른 주증상들도 없어지기 시작하였으며, 대부분(82.3%)이 주사후 20시간 이내에는 소실되었다. 주사중 3례(17.6%)에서 오한 및 일시적인 체온의 상승을 보였으나 주사를 중단할 필요는 없었다. 4. 발병 4주 이내에 관상동맥의 확장이 관찰된 경우는 8례(47.1%)였으나, 이들은 모두 경도의 확장이었다. 발병 2개월 후에 시행한 심초음파도에서는 2명(11.7%)에서만 경도의 확장을 보였고, 나머지는 모두 정상으로 회복되어 있었다. 5. 최저 헤모글로빈치, 최대 C-반응성 단백질, 최대 총백혈구수, 최고 적혈구 혈침속도, 혈소판 최고치는, 발병 4주 이내와 2개월 후에 관상 동맥의 변화가 있는 군과 정상인 군 사이에 의미 있는 차이는 없었다. 이상의 결과로 가와사끼병의 치료에 있어서 단일 고용량의 감마글로불린을 정맥주사하는 방법이 심각한 부작용 없이 임상적 증세를 빨리 호전시키며, 관상동맥의 합병증을 줄이는데 효과적임을 알 수 있었다. 따라서 모든 가와사끼병의 급성기에 가능한 빨리 단일 고용량의 감마글로불린을 정맥주사하는 것을 추천한다. The authors studied the effect of single high-dose intravenous gammaglobulin(2 gm/㎏) on the clinical symptoms and coronary artery abnormalities in seventeen Kawasaki patients and obtained the following results. 1. The patients' ages ranged from 3-month to 5-year, with mean age of 2.03-year. The patients under 2-years comprised 58.8%. The ratio of male to female was 1.125. 2. All of the patients showed 6 major diagnostic criteria with one exception of cervical lymphadenopathy(64.7%). The disease occurred mainly spring and winter season(76.5%). 3. Fever subsided within 20 hours' of intravenous gammaglobulin infusion in most of the patients(82.3%) with mean value of 11.4 hours, Transient side effects such as fever and chilling sense, developed in 3 cases(17.6%), but there was no need to quit infusion. 4. Echocardiographically, mild coronary arterial dilation was observed in 8 cases(47.1%) within 4 weeks' of onset. After 2 months' of onset, only 2 cases(11.7%) showed mild dilation. 5. There were no significant difference between coronary arterial dilation group and normal group before 4wk and after 2 mo of onset, in terms of hemoglobin nadir, maximal CRP, maximal WBC count, peak ESR, and peak platelet count. Conclusively, single high dose intravenous gammaglobulin infusion in Kawasaki disease showed effective improvement of clinical symptoms and decrease of coronary arterial abnormality. There fore all the children with Kawasaki disease should be treated with single high-dose intravenous gammaglobulin infusion as soon as possible.

구강에 발생한 편평상피암종의 MMP-2, MMP-9, TIMP-1, TIMP-2 발현과 EGFR 및 E-cadherin 발현의 상호연관성

조성재,권창석,김용환,윤혜경,김우형,이희철 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.4

Objective : Matrix metalloproteinases(MMPs) are involved in the degradation of extracellular matrix, which is related to infiltrative growth and metastasis of tumor. Positive correlation between MMPs expression and clinico-pathologic parameters were reported, and MMPs expressions were regulated by tissue inhibitor of metalloproteinases(TIMPs) or cell adhesion molecules such as E-cadherin and epidermal growth factor receptor EGFR). The aim of this study is to evaluate the relationship between MMP-2, MMP-9 expressions and clinico-pathologic factors such as clinical stage. recurrence. histologic grade and invasion pattern, and between MMP-2 and MMP-9 Expressions and TIMP-1, TIMP-2, and E-cadherin expressions. Methods and Material : The materials were 55 cases of squamous cell carcinoma of the oral cavity, and immunohisdtochemical studies for MMP-2, MMP-9, TIMP-1, TIMP-2, E-cadherin and EGFR were performed. Clinico-pathologic factors such as clinical stage, recurrence, histologic grade and invasion pattern were recorded through microscopic examination and clinical record review. Statistical analyses between MMP-2, MMP-9 expressions and clinico-pathologic factors, TIMP-1, TIMP-2 expressions and EGFR and E-cadherin expressions were done. Results : 1. MMP-2 and MMP-9 expressions were noted in 30(54.5%) and 22(40.0%) of 55 cases, respectively. TIMP-1 and TIMP-2 expressions were observed in 21(38.2%) and 33(60.0%), and E-cadherin and EGFR expressions were found in 35(63.6%) and 26(47.3%) of 55 cases, respectively. 2. MMP-2 expression rate was slightly higher in cases without recurrence, and TIMP-2 expression rate was slightly higher in cases showing more infiltrative growth pattern. However, there were no significant differences of MMP-2, MMP-9, TIMP-1, and TIMP-2 expression rates according to other clinico-pathologic parameters were noted. 3. The expression rate of EGFR was higher in cases with well differentiation (p=0.0047), but no positive relationship between the expression rate of E-cadherin and histologic grade was found. There were no significant differences of the expression rates of E-cadherin and EGFR according to other clinico-pathologic parameters. 4. Cases with positive reaction for MMP-9 showed an increasing tendency of negative reaction for TIMP-1. However, signigicant relationships between MMP-2, MMP-9 expressions and TIMP-1, TIMP-2 expressions were not noted. 5. The expression rate of MMP-2 was higher in cases with positive reaction for Ecadherin and EGFR with no statistical significance. The expression rate of MMP-9 was significantly higher in cases with positive reaction for E-cadherin(p=0.0221), but there was no significant relationship between MMP-9 and EGFR expression. Conclusion : These results suggest that MMP-2, MMP-9, TIMP-1 and TIMP-2 expressions are involved in the development of oral squamos cell carcinomas, but MMP-2, MMP-9, TIMP-1, and TIMP-2 expressions might not seem to be a sueful prognostic factors because there were no significant relationship between clinicopathologic parameters, EGFR expression showed positive correlation with low histologic grace, so EGFR expression could be regarded as a good prognostic factor. In the progression of squamous cell carcinoma of oral cavity, MMP-9 and E-cadherin might be involved with positive correlation, but there were no significant correlations between MMP-2, MMP-9 and TIMP-1, TIMP-2 and EGFR expressions rate. Further study for other factors such as cytokines or growth factors except TIMP-1, TIMP-2 and EGFR will be needed.

폐굴껍질을 산성토양 적용시 토양의 화학적 특성변화에 관한 기초 연구

임진희,문종익,김성우,성낙창,이영형,윤태경 동아대학교 환경문제연구소 1999 硏究報告 Vol.22 No.2

Oyster shell has caused environmental problems in the coast. But it is possible to be used as an acidic soil amendment because it is the alkalic material of pH 9.0. In order to evaluate the utility of the crushed oyster shell as a soil amendment, acidic sandy loam soil was amended with it and then Chinese cabbages were cultivated in pots. The amount of the oyster shell was ⅰ)none, ⅱ)0.5kg/㎥, ⅲ)1.0kg/㎥, ⅳ)1.5kg/㎥, ⅴ)2.0kg/㎥ and ⅵ)2.3kg/㎥. The particle size of oyster shell consists of 34.88% of 40∼100mesh and 14.98% of larger than 100 mesh. It increased pH and the contents of available P₂O_(5) and SiO₂ and exchangeable Ca in used soil The application of oyster shell also increased the height and diameter of Chinese cabbages.