Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

Clinicopathological role of kidney injury molecule-1 in immunoglobulin A nephropathy

( Yu Ho Lee ),( Yang-gyun Kim ),( Sang-ho Lee ),( Ju-young Moon ),( Kyung-hwan Jeong ),( Tae-won Lee ),( Chun-gyoo Ihm ) 대한신장학회 2014 Kidney Research and Clinical Practice Vol.33 No.3

Background: Urinary kidney injury molecule-1 (KIM-1) is an early and sensitivebiomarker of acute kidney injury, but it is unclear if it is a biomarker of chronicglomerulonephritis. We evaluated whether urinary KIM-1 levels in patients withimmunoglobulin A (IgA) nephropathy can be a marker to reflect clinicopathologicalseverity and predict the prognosis. Methods: We measured urinary KIM-1 levels in 40 patients (15 males; mean age36.6712.9 years) with IgA nephropathy and 10 healthy people (5 males; mean age37.379.6 years) as controls. The correlation of urinary KIM-1 levels with patients’clinical parameters, histological grades, and follow-up data were analyzed using themodified H. S. Lee grading system and tubulointerstitial change scores. Results: Urinary KIM-1 levels were higher in patients with IgA nephropathy thanhealthy controls (P¼0.001). Univariate and multivariate regression analyses showedthat urinary KIM-1 levels had a direct correlation with H. S. Lee grade andtubulointerstitial inflammation (P¼0.004 and P¼0.011, respectively). Conclusion: In patients with IgA nephropathy, urinary KIM-1 has a significantcorrelation with histopathologic severity.

김진균의 산수도

김진균 (Jin Gyun Kim) 민족음악학회 1997 음악과 현실 Vol.13 No.-

N/A

자궁내막에 발생한 점막 연관 림프조직 (MALT) 림프종 1예

전균호,조현진,박성균,김천복,김대연,김종혁,김용만,김영탁,목정은,남주현 대한부인종양 콜포스코피학회 2003 Journal of Gynecologic Oncology Vol.14 No.4

Non-Hodgkin's 림프종의 약 40%는 결절외(extranodal) 림프종으로 대개 위장관에서 발생하며 이들 중 점막 연관 림프조직(mucosa-associated lymphoid tissue: MALT)에서 기원한 림프종은 MALT 림프종으로 분류된다. 위 외의 부위에서 원발성으로 발생하는 MALT 림프종은 매우 드물지만 실제 거의 모든 신체 장기에 발생하는 것으로 알려져 있는데, 가장 흔한 부위인 위 외에도 폐, 갑상선, 침샘, 눈물샘에 발생하고, 드물게 안구, 유방, 방광, 신장, 흉선 등에도 보고된 바 있다. MALT 림프종은 오랜 기간동안 원격 전이하지 않고 국소 병변으로 남아 있는 특징이 있어 치료 방향 또한 국소 병변의 치료에 집중되며, 예후도 림프절 기원의 림프종에 비해 양호한 것으로 알려져 있다. 본 저자 등은 자궁 내막에 원발성으로 발생한 MALT 림프종 1예를 경험하였기에 문헌 고찰과 함께 이를 보고하고자 한다. Mucosa associated lymphoid tissue (MALT) lymphoma can rarely develop primarily in extranodal sites other than stomach which is the most common site for it. Other rare primary sites are small intestine, colorectum, esophagus, lung, thyroid, salivary gland, lacrymal gland, breast and skin. MALT lymphoma represents a distinct clinicopathologic features: it is usually localized to their original site for a long time and shows much more favorable prognosis than lymphoma at other site, but some MALT lymphoma can arise simultaneously or successively in different organ or give rise to another MALT lymphoma of other organ and can be multifocally disseminated or recurred. We report a very rare case of high grade B-cell lymphoma of mucosa associated lymphoid tissue (MALT) of the uterine endometrium, which was confirmed by immunohistochemical assay.

비행청소년과 일반청소년의 도덕발달 차이연구

김상균(Kim Sang Gyun) 한국공안행정학회 2005 한국공안행정학회보 Vol.20 No.-

The purpose of the present study were to compare of moral development level between delinquent adolescences and normal adolescences, and the help-ful date in understanding and guiding the delinquent adolescences. Based on the previous studies and Kohlberg's Moral development theory, it was hypothesized that there are difference of total and subfactoric moral development degree between delinquent adolescences and normal adolescences. Subject for the study were 298 boys(middle school:48/high school: 120/delinquent : 130) in Dae-Jeon. They were examine in Kohlberg's Moral development scale which rewrited by Kim Sang Gyun. Statistical methods applied for the present study was mean, standard deviation, and t-test. The statistical computation were done by SPSS 8.0 computer programs. Following conclusions were derived from the results and the discussion. 1. Hypothesis 1 was accepted. Delinquent adolescences had high score on moral development's means compare with normal adolescences. 2. Hypothesis 2 was partially accepted. Delinquent adolescences showed lower "stage 1, stage 2, stage 3" than normal adolescences. However, there were no significant differences of " stage 4, stage 5 and stage 6 " 최근 학교폭력, 청소년 성매매, 마약, 가출 등 청소년에 의한 범죄와 비행이날로 증가하고 있다.1) 이러한 상황은 자라나는 청소년들에게 급변하는 사회환경에 적절하게 대응할 수 있는 올바른 가치관과 도덕성 함양기능을 제대로 수행하지 못한 결과가 아닌가 생각한다. 최근 일각에서 청소년을 대상으로 한 인성교육의 중요성이 부각되고 있는 것은 다행스런 점이 있다. 이 논문은 Kohlberg(1976)의 도덕발달이론을 논거로 하여 비행청소년과 정상청소년간의 도덕적 추론능력의 차이를 비교한 연구이다. 그에 의하면 도덕의 발달단계에 따라 도덕적 지향의 형태가 달라지고, 도덕적 판단력의 형성에 있어서의 차이는 도덕적 행위나 비도덕적 행위에 관련이 있다고 한다. 논문에서 비행청소년의 도덕적 추론능력을 검증한 결과, 비행청소년은 전인습적 도덕성에 속하는 1, 2단계에 속하는 것으로 밝혀졌다. 또 이를 정상청소년을 준거집단으로 한 비교분석을 해 본 결과, 전인습적 도덕성에 속하는 1단계에서 유의한 차이가 있음이 밝혀졌으며, 이 외에 인습적 도덕성에 속하는 대인간의 조화와 순종의 단계에서도 유의한 차이가 발견되었다. 3단계인 대인간의 조화와 순종단계에서도 정상청소년과 유의한 차이가 있다는 의미는 비행청소년이 대인관계의 기술면에서 부족하다는 것을 나타낸다. 그 외의 단계에서는 비행청소년과 정상 청소년 간에 유의한 차이가 발견되지 않았지만 정상청소년은 콜버그의 도덕적 발달단계에서 상위단계에서도 비교적 높은 점수를 나타내고 있음을 볼 수 있다. 이러한 연구결과는 청소년을 대상으로 도덕성 함양교육 내지 인성교육이 중요함을 시사하고 있다.

비행청소년과 일반청소년의 도덕발달 차이연구

김상균(Kim Sang Gyun) 한국공안행정학회 2005 한국공안행정학회보 Vol.21 No.-

최근 학교폭력, 청소년 성매매, 마약, 가출 등 청소년에 의한 범죄와 비행이날로 증가하고 있다.1) 이러한 상황은 자라나는 청소년들에게 급변하는 사회환경에 적절하게 대응할 수 있는 올바른 가치관과 도덕성 함양기능을 제대로 수행하지 못한 결과가 아닌가 생각한다. 최근 일각에서 청소년을 대상으로 한 인성교육의 중요성이 부각되고 있는 것은 다행스런 점이 있다. 이 논문은 Kohlberg(1976)의 도덕발달이론을 논거로 하여 비행청소년과 정상청소년간의 도덕적 추론능력의 차이를 비교한 연구이다. 그에 의하면 도덕의 발달단계에 따라 도덕적 지향의 형태가 달라지고, 도덕적 판단력의 형성에 있어서의 차이는 도덕적 행위나 비도덕적 행위에 관련이 있다고 한다. 논문에서 비행청소년의 도덕적 추론능력을 검증한 결과, 비행청소년은 전인습적 도덕성에 속하는 1, 2단계에 속하는 것으로 밝혀졌다. 또 이를 정상청소년을 준거집단으로 한 비교분석을 해 본 결과, 전인습적 도덕성에 속하는 1단계에서 유의한 차이가 있음이 밝혀졌으며, 이 외에 인습적 도덕성에 속하는 대인간의 조화와 순종의 단계에서도 유의한 차이가 발견되었다. 3단계인 대인간의 조화와 순종단계에서도 정상청소년과 유의한 차이가 있다는 의미는 비행청소년이 대인관계의 기술면에서 부족하다는 것을 나타낸다. 그 외의 단계에서는 비행청소년과 정상 청소년 간에 유의한 차이가 발견되지 않았지만 정상청소년은 콜버그의 도덕적 발달단계에서 상위단계에서도 비교적 높은 점수를 나타내고 있음을 볼 수 있다. 이러한 연구결과는 청소년을 대상으로 도덕성 함양교육 내지 인성교육이 중요함을 시사하고 있다. The purpose of the present study were to compare of moral development level between delinquent adolescences and normal adolescences, and the helpful date in understanding and guiding the delinquent adolescences. Based on the previous studies and Kohlberg's Moral development theory, it was hypothesized that there are difference of total and subfactoric moral development degree between delinquent adolescences and normal adolescences. Subject for the study were 298 boys(middle school:48/high school: 120/delinquent : 130) in Dae-Jeon. They were examine in Kohlberg's Moral development scale which rewrited by Kim Sang Gyun. Statistical methods applied for the present study was mean, standard deviation, and t-test. The statistical computation were done by SPSS 8.0 computer programs. Following conclusions were derived from the results and the discussion. 1. Hypothesis 1 was accepted. Delinquent adolescences had high score on moral development's means compare with normal adolescences. 2. Hypothesis 2 was partially accepted. Delinquent adolescences showed lower "stage 1, stage 2, stage 3" than normal adolescences. However, there were no significant differences of " stage 4, stage 5 and stage 6"

요추 추간판 탈출증에서 현미경을 이용한 추간판 절제술 : 5년 추시 결과 A 5-years follow up

심대무,김상수,김태균,송하헌,최시호 대한척추외과학회 2000 대한척추외과학회지 Vol.7 No.1

목적 : 저자들은 요추 추간판 탈출증으로 진단받고 고식적 추간판 절제술과 현미경을 이용한 추간판 절제술을 시행한 환자를 대상으로 수술전후 임상결과를 파악하고 현미경을 이용한 수술의 장기 추시결과와 효율성을 알아보자하였다. 대상 및 방법 : 1988년 1월부터 1993년 12월까지 요추 추간판 탈출증으로 진단받고 고식적 방법 및 현미경을 이용한 방법으로 추간판 절제술을 시행하고 5년이상 추시 관찰이 가능하였던 각각 30명의 환자를 대상으로 관찰군과 비교군으로 설정하고 환자의 연령 및 성별분포, 발병 원인과 증상 및 진단과 이환부위에 대하여 분석하고 임상적 결과를 김의 방법으로 평가하고 paired T test를 이용한 통계학적 검증을 하였다. 결과 : 수술시간은 고식적 수술의 경우 평균 117분, 현미경을 이용한 경우 98분이 걸렸으며 직업복귀 까지는 고식적 추간판 절제술시 9.6주, 현미경을 이용한 경우 5.9주가 소요되었다. 임상평가 결과는 술후 1년이상 추시상 고식적 방법에서 양호이상이 76.9%이었으며 현미경을 이용한 경우는 양호이상이 84.6%를 보였다. 5년이상 추시상 고식적인 방법에서 60.0%와 현미경을 이용한 경우 63.3%로 비슷한 결과를 보였다. 결론 : 현미경을 이용한 요추 추간판 절제술시 정상조직에 대한 최소한의 손상과 신경근의 명료한 구분으로 안전하게 시행할 수 있는 방법으로 사료되며 장기적 추시결과에서도 고식적 수술방법과 유사한 결과를 보여 요추 추간판 탈출증 환자의 수술적 치료의 한 방법으로 환자들의 치료에 도움이 되리라 사료되었다. Purpose : This study was performed in an attempt to determine if there was any clnical benefit of microdiscectomy(MD) over standard discectomy(SD). They were all followed up by an impartial observer at 1 year and 5 years. Materials and Methods : All patients were operated on by the same surgeon by either method. We evaluate retrospectively 30 cases of microdiscectomy and 30 cases of microdiscectomy and 30 cases of standard discectomy using data derived from a questionnaire and chart review from January 1. 1988 to December 31. 1993. The operative results were analysed with Kim's creteria and that clinical results were statistically used to Paired two-tailed T test. Results : 1)Mean operating time was about 117 minutes in the standard discectomy, while 98 minutes in the microdiscectomy. 2)Mean time to returm to work was about 9.6 weeks in the standard discectomy, 5.9 weeks in the microdiscectomy. 3)In initial and 1 year follow up, microdiscectomy was superior to the standard discectomy but in 5 years follow up, the two procedures have a similar outcome. Conclusion : The advantage of microdiscectomy was more safe than standard discectomy, because it was mahinfied vision and brilliant illumination, precise identification of structures in deep fields(including nerve root and its related structures), a marked adantage to dissect the adhere nerve root to its surroundings structures, its capacity to preserve the integrity of normal tissue, and meticulous hemostasis. From this analysis, we conclude that microdiscetomy represents a small but significant refinement of standard discectomy

Meso-Dihydroguaiaretic Acid Isolated from Saururus chinensis Inhibits Cyclooxygenase-2 and 5-Lipoxygenase in Mouse Bone Marrow-Derived Mast Cells

Tae Chul Moon,Chang Seob Seo,Kyungmi Haa,Jin Cheul Kim,Nam Kyung Hwang,Tae Gyun Hong,Jee Hyeun Kim,Do Hun Kim1,손종근,장현욱 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.5

Meso-dihydroguaiaretic acid (MDGA) is a medicinal herbal product isolated from the aerial parts of Saururus chinensis that inhibits the cyclooxygenase-2 (COX-2)-dependent phase of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) (IC50 9.8 μM). However, this compound did not inhibit COX-2 protein expression in BMMC at concentrations up to 30 μM, indicating that MDGA directly inhibits COX-2 activity. In addition, this compound consistently inhibited the production of leukotriene C4 (IC50 1.3 μM). These results demonstrate that MDGA inhibits both COX-2 and 5-lipoxygenase. Furthermore, this compound strongly inhibited the degranulation reaction in BMMC (IC50 11.4 μM). Therefore, this compound might provide a basis for novel anti-inflammatory drug development.

Original Articles : Clinical significance of occult hepatitis B virus infection in chronic hepatitis C patients

( Jae Young Jang ),( Soung Won Jeong ),( Sung Ran Cheon1 ),( Sae Hwan Lee1 ),( Sang Gyune Kim ),( Young Koog Cheon ),( Young Seok Kim1 ),( Young Deok Cho1 ),( Hong Soo Kim ),( So Young Jin ),( Yun Soo 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3

Background/Aims: We investigated the frequency of occult hepatitis B virus (HBV) infection in anti-hepatitis C virus (HCV)-positive individuals and the effects of occult HBV infection on the severity of liver disease. Methods: Seventy-one hepatitis B virus surface-antigen (HBsAg)-negative patients were divided according to their HBV serological status into groups A (anti-HBc positive, anti-HBs negative; n=18), B (anti-HBc positive, anti-HBs positive; n=34), and C (anti-HBc negative, anti-HBs positive/negative; n=19), and by anti-HCV positivity (anti-HCV positive; n=32 vs. anti-HCV negative; n=39). Liver biopsy samples were taken, and HBV DNA was quantified by real-time PCR. Results: Intrahepatic HBV DNA was detected in 32.4% (23/71) of the entire cohort, and HBV DNA levels were invariably low in the different groups. Occult HBV infection was detected more frequently in the anti-HBc-positive patients. Intrahepatic HBV DNA was detected in 28.1% (9/32) of the anti-HCV-positive and 35.9% (14/39) of the anti-HCV-negative subjects. The HCV genotype did not affect the detection rate of intrahepatic HBV DNA. In anti-HCV-positive cases, occult HBV infection did not affect liver disease severity. Conclusions: Low levels of intrahepatic HBV DNA were detected frequently in both HBsAg-negative and anti-HCV-positive cases. However, the frequency of occult HBV infection was not affected by the presence of hepatitis C, and occult HBV infection did not have a significant effect on the disease severity of hepatitis C. (Korean J Hepatol 2011;17:206-212)

응급 수술을 시행한 복부 외상 환자의 예후 인자는 무엇인가?

김균무 고신대학교 의과대학 2011 고신대학교 의과대학 학술지 Vol.26 No.1

OBJECTIVES: To study epidemiologic characteristics and prognostic factors of emergency laparotomy patients due to abdominal trauma. METHODS: From Jan 2006 to Dec 2008, 116 patients enrolled in this study. Inclusion criteria for patients were abdominal trauma patients with emergency laparotomy. Age, sex, ED access, transportation, previous history, time, injured organ, combined injury, initial mental status, revised trauma score, laboratory finding, mortality ware reviewed. RESULTS: Trauma was most common at 40s and at night. Men has three time more common than women. Trauma etiology was traffic accident (64 cases, 55.2%), stab injuries (31 cases, 26.7%), violence (11 cases, 9.5%). Most commonly injured organs were the small intestine, the mesentery was a following. Combined injuries were extremities fractures, head and neck injuries, chest trauma, pelvis trauma, respectively. The average time of prehospital interval was 186.49 +/- 233.29 minutes and Emergency department stay time was 270.50 +/- 180.08 minutes. The average hospitalization was 22.84 +/- 38.02 days. In a univariate logistic regression study, age (odds ratio [OR]: 1.364), systolic blood pressure below 90mmHg (OR 4.460), Hemoglobin (OR 2.380), Revised trauma score (OR 0.902), initial mental status (OR 3.394) were significant prognostic factor. CONCLUSIONS: The result of this study indicated that age, systolic blood pressure below 90mmHg, hemoglobin, revised trauma score, initial mental status were prognostic factor of emergency laparotomy patients due to abdominal trauma. When treating patients with abdominal trauma, we could be considered these prognostic factors. In addition, to establish therapeutic plan will be helpful.