흰쥐 시상에서 Epidermal growth factor receptor면역반응 신경세포의 생후 발달에 관한 연구

박영란,정윤영,김종중,문정석,오재욱,정영욱,김주수 조선대학교 2003 The Medical Journal of Chosun University Vol.28 No.1

Background and Objectives : Epidermal growth factor receptor (EGFR), a 170-kDa transmembrane glycoprotein, appears to mediate epidermal growth factor (EGF) activity. Transforming growth factor-α and EGF produce their biological effects in numerous systems by stimulating the EGFR In this study, we examine the postnatal development of EGFR immunoreactivity in the different regions of the thalamus of the rat Materials and Methods : The present study is based on 28 postnatal cases of rat thalamus ranging from the day of birth, postnatal day 0 (P0) to 30 days (P3, P5, P10, P15, P20, P30), and these cases were compared with adult rat thalamus. Cryostat sections were processed free-floating with monoclonal antibody by immunohistochemistry Results : EGFR immunoreactivity in the thalamus of the rat showed very different patterns according to postnatal ages and thalamic areas. EGFR-immunoreactive cells appeared in the first two postnatal weeks, except the ventral posterior thalamic nuclei. In the early postnatal days, EGFR-immunoreactive cells appeared thalamic midline structures, increased progressively in the first two postnatal weeks, and followed mediolateral gradient. The mature patterns of EGFR-immunoreactive cells were achieved at P20 Conclusion : These data indicate that the maturation of EGFR-immunoreactive cells requires a relatively prolonged period of time to achieve an adult configuration. Many growth factors probably play protective or neurotrophic roles at EGFR-immunoreactive neurons of thalamus both young and adult rats In addition to difference in time of appearance in thalamic nuclei and developing pattern with mediolateral gradient suggest that EGFR-immunoreactivities are correlated with the appearance of the related functional.

모체 Thyroxine 투여가 태아알코올효과를 가진 흰쥐 대뇌겉질 및 해마에서 NPY함유 신경세포의 생후 발달에 미치는 영향

김복,박상기,박영란,김종중,문정석,김주수,문영민,현영식,천관영,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: Maternal alcohol abuse is considered to be one of the most prominent cause of neurobiological malformations in the postnatal and adult life of the offspring. In this study, we investigated the effects of maternal alcohol drinking on the postnatal development of NPY-containing neuron, and, the influence of thyroxine treatment on the cerebral cortex and hippocampus of pups of alcohol abused mother. Materials and Method: Time-pregnant rats were divided into three groups. Alcohol-fed group A received 35 calories of liquid alcohol diet daily from gestation day 6; control pair-fed group B was fed a liquid diet in dextrin replaced alcohol isocalorically: alcohol + T4 group C received 35 calories liquid alcohol diet and exogenous thyroxine subcutaneously. Results: Group C showed prominent NPY immunoreactivity in the cerebral cortex compared to group A and B at P7. In group C, NPY-containing neurons were widely distributed in the all layers of cerebral cortex after P14. Besides, numerical decrease of NPY-containing neuron as age increases was not found in group C. However, the decrease of NPY-containing neuron was clearly observed in group A compared to group C after P14. In hippocampus, group Band C were appeared similar patterns after P7. Additionally, in group C, NPY immunoreactivity was prominently appeared in CA2 and CA3 at P14 as compared to group B. Conclusions: The present results showed the increase of intensity and number of NPY-containing neurons in cerebral cortex and hippocampus of pups of exogenous T₄ supplemented alcohol-exposed dams as compared to control pair-fed and alcohol-exposed pups at P7. It presumably suggest that NPY-containing neurons might be regulated by the early postnatal growth stimulatory effect of the exogenously supplemented T₄. Therefore, the increase of NPY synthesis caused by maternal administration of exogenous thyroxine may ameliorate fetal alcohol effect, one of the ill effects as a result of the dysthyroid state following maternal alcohol abuse.

지난 2년간의 학회지 "신경정신의학" 심사 평가보고

김찬형,김세주,김도관,김성곤,박원명,송동호,박용천,이영문,오강섭,정영철,이상열,김주한,이동영,이헌정,함병주,김율리,김문두,김정진,이강욱,천근아,김재진,홍진표,홍승철 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.6

여성잡지에 나타난 식품영양정보의 양적·질적 현황 조사 (2002년)

문현경,용미진,장영주 대한영양사협회 2004 대한영양사협회 학술지 Vol.10 No.2

The desire for the healthy life is increasing as a result of rapidly changing social and economic environment in Korea. Because of the need, information on health, food and nutrition are being introduced through the mass media. The object of this study is to investigate and analyze the quantity and quality of information about health, food and nutrition in women's magazines. Six representative domestic monthly magazines(Lady Kyunghyang, Women Donga, Women Chosun, Women JoongAng, Women sense, Jubu Senghwal) were selected for this study. The results of monitoring from June 2002 to September 2002 for the women's magazines were summarized as follows; 1. The percentage of the articles on health, food and nutrition by total area of the women's magazines was 23.1%. There were total 595 articles on health, food and nutrition. Among these, 400 articles(67.2%) were about food and nutrition. 2. The percentage of the advertisements on health, food and nutrition was 35.3% by total area of the woman's magazines. There were 2,353 advertisements on health, food and nutrition. Among these, 1,574(66.9%) advertisements were about food and nutrition. The most frequently advertised advertisements on food and nutrition were about processed foods (527 cases, 33.5%). The second most frequently advertised advertisements were about diet (374 cases, 23.8%). The third one was healthy foods (345 cases, 21.9%). 3. The contents of 215(53.8%) articles (the total number of articles was 400) was suitable. The contents of 89 advertisements(22.3%) did not have accurate information on health, food and nutrition. For the advertisements in women's magazines (the number of total articles was 2,203), the percentage of the advertisements which was not able to capture the feature of the advertisements was 27.9%, insufficiency of reliable sources was to 21.9%, and exaggeration of the contents in the advertisements was 16.0%. Articles and advertisements should be backed by scientific research or reliable sources and also the opinions of people with expertise in order to report accurate information to the general public. In order to achieve these results, there should be continuing monitoring activity for the women's magazines.

肺癌 自動 診斷을 위한 血管-Tree 構成의 改善 : 再歸的 方法에 의한 Threshold의 使用 Using The Threshold Deteshold Detected by Recursive Method

문주영 부천대학 2001 論文集 Vol.22 No.-

본 논문에서는 폐암 단체 검진에 대비한 CT 이미지의 1차 스크리닝(Screening) 자동화 시스템(Computer Assisted Diagnosis system)의 개선방법을 제안한다. 2차원의 CT 이미지로부터 3차원적인 정보를 수집(detect)하여 종양으로 의심할 수 있는 이상영역을 추출(extract)하는 방법을 제안한다. 즉, 각 슬라이스에 대하여 2차원 CT 이미지의 상하 슬라이스의 관계를 규명함으로써 3차원의 CT 이미지의 정보를 수집하고, 정상영역에서 제외된 영역을 이상영역(abnormal area)으로 정의하는(extract) 방법이다. 2차원적인 처리 단계에서의 CT 이미지에서 종양영역을 찾아내기 위한 유일한 실마리는 CT값이라 할 수 있지만, 획일된 CT값의 Threshold만으로는 종양영역을 찾기가 매우 어렵고 정확하지 못하다. 그것은 혈관영역의 CT값이 종양영역과 거의 일치하기 때문이다. 더우기 CT값은 주변 물질에 의해 영향을 받기 때문에 각 슬라이스의 위치를 고려한 가변적(varaiable)이고 재귀적(recursive)인 방법으로 Threshold를 결정해야 한다. 이에 따라 본 논문에서는 CT 이미지의 1차 스크리닝(Screening) 자동화 시스템을 개선하기 위하여 재귀적으로 구한 Threshold를 적용시키는 방법을 제안한다. In this paper, we propose an improvement of the computer-assisted diagonosis system for the first screening of CT images, preparing for a mass health examination for the lung cancer. The essence of the improving method is to extract abnormal areas suspected as cancers, by detecting three-dimensional information from two-dimensional images, that is, we collect information of three-dimensional CT images by investigating the relation of connected slices of two-dimensional CT images for each slice and extract areas excluded from the normal area as abnormal ones. While the only clue to find out cancer areas at the stage of two-dimensional processing is the CT values, it is very difficult and inaccurate to find out the tumor area from the threshold of CT values alone, This is because the CT values of vessels are almost the same as those of cancers. Furthermore, since the CT values depend on surrounding matters we must determine the threshold in a variable and recursive way, considering the position of each slice. Accordingly, in order to improve the computer-assisted diagnosis system for the first screening of CT images, we propose an using the threshold detected by recursive method.

임상목회교육 수퍼비전과 영성에 관한 고찰

문영주 연세대학교 신과대학 2008 신학논단 Vol.54 No.-

This paper specifically examines in what form and through what process changing of spiritual understanding of supervisee appears, and argues for its causes and factors. This paper also shows what form and content of supervision as well as what supervisory acts influences change of spiritual understanding of the supervisee. On the basis of these observations I aim to supply basic data to prepare for a plan that can promote changing of spiritual understanding of supervisee in Pastoral counseling supervision. In order to achieve the purpose of study, I try to see and understand changing of spiritual understanding from a supervisee's perspective, I choose to use the 'Grounded Theory Method' of Strauss and Corbin (2001) out of some other methods of the Qualitative Study to express the experience of a special and private changing of spiritual understanding of supervisee and the experience of their supervision. Data are collected by 'In-depth interview investigation' and 'Participation observation method'. The total number of supervisees is 15; 3 supervisees in their twenties, 6 in their thirties, 4 in their forties, and 2 in their fifties. Four of them are Roman Catholics, and the other 11 are Protestants, all of them being seminary students and clergymen. In this study, I observe 'narrow-minded understanding on death and disease' and 'bitter experiences of inhospitality' as major igniting factors of changing of spiritual understanding, and present 'supervisor's way of treating supervisees' and 'experience of holiness' as major strengthening factors.

일간 신문의 기사와 광고에 나타난 식품영양정보의 현황 조사 (2002년)

문현경,용미진,장영주 대한영양사협회 2004 대한영양사협회 학술지 Vol.10 No.2

The object of this study is to investigate and analyze the quantity and quality of information on health, food and nutrition reported by newspapers. Six different major domestic daily newspapers(Hankook Ilbo, Donga Ilbo, Chosun Ilbo, Kyunghyang Shinmun, Hankyoreh Shinmun, JoongAng Ilbo), were monitored from 1st of May to 31st of Oct. 2002. The results of monitoring in the newspapers were summarized as follows; 1. The total percentage of the articles on health, food and nutrition was 2.7% and the percentage of the advertisements on health, food and nutrition was 17.5% of total area of the newspaper. The ratio of the number of food and nutrition topic on the total number of health, food and nutrition topic was 35.8% for the articles, was 62.9% for the advertisements. Among advertisements on food and nutrition, the percentage of advertisements on healthy foods was the highest(3,481 or 55.0%). 2. Contents of 340 articIes(26.1%) were reported as suitable information. Contents of 259 articles(l9.9%) were reported as inaccurate information on health, food and nutrition. In the analysis of advertisements, the number of advertisements without sufficient reliable sources was 2,488 cases(23.0%), and with exaggerated contents was 2,268 cases(21.0%). The articles and advertisements should be backed by scientific research or reliable sources and also the opinions of people with expertise in order to report accurate information to the general public. In order to achieve these results, there should be continuing monitoring activity for the newspapers.

Inducing apoptpsis by the inhibition of c-myb in oral squamous carcinoma cell line, KB cell

Lee,Jung-Chang 대한구강생물학회 2007 International Journal of Oral Biology Vol.32 No.4

Oral squamous cell carcinoma (OSCC) is the most commonmalignancy and is a major cause of worldwide cancer mortality.The proto-oncogene c-myb plays an important role in regulationof cell growth and differentiation, and it is expressed at highlevels in hematopoietic cells and many other types of cancers.However, the function of c-myb is not well known in OSCC.The present study aimed to reveal the function of c-myb and totest the alternation of cell growth and signaling by c-myb inOSCC. In this study, c-myb and dominant-negatibe myb(DN-myb) were expressed in an adenovirus-mediated gene deliverysystem to KB cells. The over-expressed c-myb broughtincreased cellular proliferation compared with control cells.However, DN-myb infected KB cells showed significantreduction of cell growth and enhanced induction of apoptosis toactivate PARP and caspase 9. c-myb induced increase of IGF-I,-II and IGF-IR expressions while DN-myb down-regulatedthese expression. Activation of ERK and Akt/PKB pathwaywas shown only in c-myb transduced cells. These findingssuggest that the role of c-myb in cell growth of oral cancer cellsis partially mediated through the modulation of IGFs, ERK andAkt/PKB. From this results, DN-myb is strongly recommendedas a curable gene for the treatment of c-myb dependentmalignancies such as OSCC.

피부와 외래환자와 일반인의 진균질환 역학조사

문현주,문재동,이지범,김성진,원영호,이승철 全南大醫大 2001 전남의대학술지 Vol.37 No.4

상·하의 배색과 하의형태가 인상형성에 미치는 영향

문주영,강경자 한국의류학회 2003 한국의류학회지 Vol.27 No.1

The purpose of study is to investigate the effect of clothing coloring and type of pants/skirt as clothing cues on impression of college female students. The experimental materials developed for this study are a set of stimuli and response scales. Thirty two color pictures of various combinations of colors for shirt, and colors and type of pants/skirt were manipulated on computer drawing. For visual evaluation of women's casual wears about colors and type, 7-point scales were designed. The subjects consist of 192 female and male undergraduate students in Chinju city. The results of this study can be summaried as follow: As analyzing the impression of female figure by the color of shirt and the color and type of pants/skirt, five factors including appearance valuation, concentration of attention, clearness, stability, and activity were found to be important. Color of shirt have significant effects on appearance valuation, concentration of attention, clearness, stability, and activity. Color of pants and shirt also have signigicant effects on all five factors. Especially, they strongest predictors for stability and activity. Type of pants/skirt significantly effects on concentration of attention and activity. In the effects of interactions between each variable, the combination of pants colors and type of pants/skirt had significant effects on appearance valuation, stability factors. Also shirt and pants colors had an significant effects on appearance valuation, stability, and activity. But in the impression formation of concentration of attention and clearness, only the combination of shirt colors and pants forms was more significant.