Screening potential reference genes for reverse transcription-quantitative polymerase chain reaction normalization in Atrijuglans hetaohei (Lepidoptera: Gelechioidea)

Li Feifei,Li Yan,Wang Tao,Li Tianfeng,Zhu Shuying,Pei Pei,Tang Guanghui 한국곤충학회 2020 Entomological Research Vol.50 No.6

Atrijuglans hetaohei Yang (Lepidoptera: Gelechioidea), is one of the major pests that seriously damage the walnut fruits. Although the morphology and physiology of A. hetaohei have been widely studied, suitable reference genes for normalizing target gene expression have not been identified. In this study, the expressions of eight candidate reference genes including GAPDH, β-Tubulin, TATA-binding protein (TBP), Histone, Ubiquitin-conjugating enzyme 9 (UBC9), arginine kinase (AK), 28S and elongation factor 1 alpha (EF1α) in different developmental stages and various larval tissues of A. hetaohei were determined using reverse transcription-quantitative polymerase chain reaction. Moreover, the expression stability of the selected reference genes was evaluated by ΔCt method, BestKeeper, NormFinder, geNorm and ReFinder, a comprehensive software platform. These results demonstrated that the best reference genes were GAPDH and 28S at different developmental stages of A. hetaohei; and the optimum references in various larval tissues of A. hetaohei were 28S, Histone and TBP. Our study should be useful to analyze the expression profiles of target genes and form a solid foundation for future research on the understanding of the genes’ biological functions in A. hetaohei.

A New Direction of Cancer Classification: Positive Effect of Low-Ranking MicroRNAs

Feifei Li,Minghao Piao,Yongjun Piao,Meijing Li,류근호 질병관리본부 2014 Osong Public Health and Research Persptectives Vol.5 No.5

Objectives: Many studies based on microRNA (miRNA) expression profiles showed a new aspect of cancer classification. Because one characteristic of miRNA expression data is the high dimensionality, feature selection methods have been used to facilitate dimensionality reduction. The feature selection methods have one shortcoming thus far: they just consider the problem of where feature to class is 1:1 or n:1. However, because one miRNA may influence more than one type of cancer, human miRNA is considered to be ranked low in traditional feature selection methods and are removed most of the time. In view of the limitation of the miRNA number, low-ranking miRNAs are also important to cancer classification. Methods: We considered both high- and low-ranking features to cover all problems (1:1, n:1, 1:n, and m:n) in cancer classification. First, we used the correlation-based feature selection method to select the high-ranking miRNAs, and chose the support vector machine, Bayes network, decision tree, k-nearestneighbor, and logistic classifier to construct cancer classification. Then, we chose Chi-square test, information gain, gain ratio, and Pearson’s correlation feature selection methods to build the m:n feature subset, and used the selected miRNAs to determine cancer classification. Results: The low-ranking miRNA expression profiles achieved higher classification accuracy compared with just using high-ranking miRNAs in traditional feature selection methods. Conclusion: Our results demonstrate that the m:n feature subset made a positive impression of low-ranking miRNAs in cancer classification.

Identification, characterization, and expression of two novel P450 genes CYP6BQ21 and CYP6BQ22 from Dastarcus helophoroides (Coleoptera: Bothrideridae)

Li Feifei,Wang Haidong,Shaheen Tayyab,Pei Pei,Li Menglou,Tang Guanghui 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.3

Chemical pesticides often applied to effectively control the long-horned beetles to protect the forests are reported to affect the non-target organisms adversely. Dastarcus helophoroides is an active natural enemy of long-horned beetles. Studying the molecular mechanism of P450 genes will help to elucidate the metabolic mechanism of pesticides in D. helophoroides to better coordinate the use of chemical and biological controls. In this study, two novel genes, CYP6BQ21, and CYP6BQ22 were successfully cloned from D. helophoroides using the rapid ampli fication of cDNA ends technique. The sequence and homology analyses indicated that CYP6BQ21 was highly similar to CYP6BQ1 from Tribolium castaneum, while CYP6BQ22 was closely related to CYP6BQ13 from T. castaneum. Gene expression patterns showed that CYP6BQ21 and CYP6BQ22 were specifically expressed in the adult stage of D. helophoroides. In addition, CYP6BQ21 and CYP6BQ22 were significantly expressed under the treatment of a high concentration of cypermethrin. Based on these findings, we proposed that CYP6BQ21 and CYP6BQ22 played possible roles in the development and pesticide stress of D. helophoroides. Our findings are an important first step in identifying and characterizing CYP6BQ21 and CYP6BQ22 from D. helophoroides, and lay the groundwork for future research into the role of these novel CYP6s in the regulation of pesticide resistance in D. helophoroides.

Effect of Red Pepper (Capsicum frutescens) Powder or Red Pepper Pigment on the Performance and Egg Yolk Color of Laying Hens

Li, Huaqiang,Jin, Liji,Wu, Feifei,Thacker, Philip,Li, Xiaoyu,You, Jiansong,Wang, Xiaoyan,Liu, Sizhao,Li, Shuying,Xu, Yongping Asian Australasian Association of Animal Productio 2012 Animal Bioscience Vol.25 No.11

Two experiments were conducted to study the effects of red pepper (Capsicum frutescens) powder or red pepper pigment on the performance and egg yolk color of laying hens. In Exp. 1, 210, thirty-wk old, Hy-line Brown laying hens were fed one of seven diets containing 0.3, 0.6, 1.2, 2.0, 4.8 or 9.6 ppm red pepper pigment or 0.3 ppm carophyll red. Each diet was fed to three replicate batteries of hens with each battery consisting of a row of five cages of hens with two hens per cage (n = 3). In Exp. 2, 180, thirty-wk old, Hyline Brown laying hens, housed similarly to those in Exp. 1, were fed an unsupplemented basal diet as well as treatments in which the basal diet was supplemented with 0.8% red pepper powder processed in a laboratory blender to an average particle size of $300{\mu}m$, 0.8% red pepper powder processed as a super fine powder with a vibrational mill ($44{\mu}m$) and finally 0.8% red pepper powder processed as a super fine powder with a vibrational mill but mixed with 5% $Na_2CO_3$ either before or after grinding. A diet supplemented with 0.3 ppm carophyll red pigment was also included (n = 3). In both experiments, hens were fed the red pepper powder or pigment for 14 days. After feeding of the powder or pigment was terminated, all hens were fed the basal diet for eight more days to determine if the dietary treatments had any residual effects. In Exp. 1, there were no differences in egg-laying performance, feed consumption or feed conversion ratio due to inclusion of red pepper pigment in the diet. Average egg weight was higher (p<0.05) for birds fed 1.2, 2.4 or 9.6 ppm red pepper pigment than for birds fed the diet containing 0.3 ppm red pepper pigment. On d 14, egg color scores increased linearly as the level of red pepper pigment in the diet increased. In Exp. 2, feeding red pepper powder did not affect egg-laying performance, feed consumption or feed conversion ratio (p>0.05). However, compared with the control group, supplementation with all of the red pepper powder treatments increased egg weight (p<0.05). All the red pepper powder treatments also increased (p<0.05) the yolk color score compared with the control. The results of the present study suggest that both red pepper powder and pigment are effective feed additives for improving egg yolk color for laying hens.

Analysis of MicroRNA-Based Cancer Classification Using SVM Classifier

Feifei Li,Minghao Piao,Keun Ho Ryu(류근호) 한국정보과학회 2014 한국정보과학회 학술발표논문집 Vol.2014 No.6

Temperature dependent defence of Nicotiana tabacum against Cucumber mosaic virus and recovery occurs with the formation of dark green islands

Feifei Zhao,Yanan Li,Li-juan Chen,Lisha Zhu,Han Ren,Honghui Lin,Dehui Xi 한국식물학회 2016 Journal of Plant Biology Vol.59 No.3

Temperature is an important environmental factor controlling plant growth, development, and immune response. However, the role of temperature in plant disease resistance is still elusive. In the present study, the potential effects of temperature on the interaction between Nicotiana tabacum and Cucumber mosaic virus (CMV) were investigated. Our results indicated that N. tabacum plants displayed severe symptoms at early stage of post inoculation at high temperature (HT, 28°C), associated with higher viral replication level, more serious stress damages. By contrast, low temperature (LT, 18°C) effectively delayed the replication of CMV compared with elevated temperatures. Additionally, quantitative real-time PCR analyses revealed that lower temperatures (≤ 24°C) promote salicylic acid (SA) dependent responses, whereas higher temperatures (> 24°C) activate the genes expression of jasmonic acid (JA) pathway. Interestingly, the dark green islands (DGIs) appeared much earlier in CMV-inoculated plants grown at HT compared with those at LT and the accumulation of virus small interfering RNAs in plants were significantly up-regulated under elevated temperatures at early stage of post inoculation. Taken together, these results indicated that temperature changes had important effects on plant defence response, and different temperatures could induce different immune pathways of N. tabacum against CMV infection.

Ethanol dry reforming over ordered mesoporous Co-Zn composite oxide for syngas production

Feifei Li,Jiale Dong,Mingyue Wang,Xingtao Lin,Weijie Cai,Xianyun Liu 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.7

This work is mainly concerned with the synthesis of Co-Zn nanocomposites by employing MCM-41 silicaas hard template (CoZnO-HT) and its catalytic behavior toward to CO2 reforming with ethanol. The physicochemicalfeatures of the as-prepared catalysts were probed through various characterization techniques, including XRD, TEM,BET, H2-TPR, and XPS. Indeed, CoZnO-HT catalyst possessed a highly ordered mesostructure with similarity toMCM-41 template and a higher specific surface area (304m2/g) compared to the reference CoZnO-C sample (4.75m2/g) prepared by the conventional impregnation method. Consequently, CoZnO-HT exhibited good performance at lowtemperature, and full ethanol conversion could be achieved at 550 oC as well as the negligible formation of byproductacetone. In addition, this catalyst depicted good stability and no obvious deactivation was observed after 40 h time onstream tests under the stoichiometric feed ratio. Indeed, superior specific surface area and efficient mass transportwithin the mesopores might be critical factors assigned to better activity and stability for CoZnO-HT.

Quantification of Nerve Viscosity Using Shear Wave Dispersion Imaging in Diabetic Rats: A Novel Technique for Evaluating Diabetic Neuropathy

Liu Feifei,Li Diancheng,Xin Yuwei,Liu Fang,Li Wenxue,Zhu Jiaan 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.2

Objective: Viscoelasticity is an essential feature of nerves, although little is known about their viscous properties. The discovery of shear wave dispersion (SWD) imaging has presented a new approach for the non-invasive evaluation of tissue viscosity. The present study investigated the feasibility of using SWD imaging to evaluate diabetic neuropathy using the sciatic nerve in a diabetic rat model. Materials and Methods: This study included 11 diabetic rats in the diabetic group and 12 healthy rats in the control group. Bilateral sciatic nerves were evaluated 3 months after treatment with streptozotocin. We measured the nerve cross-sectional area (CSA), nerve stiffness using shear wave elastography (SWE), and nerve viscosity using SWD imaging. The motor nerve conduction velocity (MNCV) was also measured. These four indicators and the histology of the sciatic nerves were then compared between the two groups. The performance of CSA, SWE, and SWD imaging in distinguishing the two groups was assessed using receiver operating characteristic (ROC) analysis. Results: Nerve CSA, stiffness, and viscosity in the diabetic group was significantly higher than those in the control group (all p < 0.05). The results also revealed a significantly lower MNCV in the diabetic group (p = 0.005). Additionally, the density of myelinated fibers was significantly lower in the diabetic group (p = 0.004). The average thickness of the myelin sheath was also lower in the diabetic group (p = 0.012). The area under the ROC curve for distinguishing the diabetic neuropathy group from the control group was 0.876 for SWD imaging, which was significantly greater than 0.677 for CSA (p = 0.030) and 0.705 for SWE (p = 0.035). Conclusion: Sciatic nerve viscosity measured using SWD imaging was significantly higher in diabetic rats. The viscosity measured using SWD imaging performed well in distinguishing the diabetic neuropathy group from the control group. Therefore, SWD imaging may be a promising method for the evaluation of diabetic neuropathy.

Cloning and Sequence Analysis of Nitrate Reductase Gene cDNA Fragments from Non-Heading Chinese Cabbage

Feifei Sun,Xilin Hou,Ying Li 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Opportunities, Challenges and Tactics for Communication of Sports Academic Journals in the Era of AI and Multi-Media

Feifei Song,Aiqun Li,Yingya You 아시아건강운동학회 2023 Journal of Asian Society for Health & Exercise Vol.5 No.1

PURPOSE : With the advent of the AI and multi-media in 21st century, the innovation of media technology has brought new opportunities and challenges to the communication of academic journals. RESULTS : The study concluded that AI + multi-media can help sports academic journals realize the efficient production of academic papers, the diversified publication of academic achievements, and the precise distribution of academic ideas. CONCLUSIONS : It is also necessary to pay attention to the challenges, which are the accelerated imbalance in academic publishing ecology, slow enhancment of academic attractiveness, and becoming fomality in academic communication. The authors also puts forward the tactics, such as balancing technical rationality and value rationality, promoting the integration of technology and content, and strengthening the depth of accurate communication.