RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

Clothing Pressure of selected Support Panty Stockings

(Jeong Eun Park),(Su Kwang Sung),(Min Kyu Song) 한국의류산업학회 2000 한국의류산업학회지 Vol.2 No.5

The purpose of the study was to obtain the basic data on the self-adjusting ability of the support panty stockings. Ten healthy women in the twenties were selected as subjects and clothing pressure of 9 support panty stockings made of single covered yarn (SCY) and double covered yarn (DCY) were measured under the standard environmental condition for the study. Data was analysed statistically according to body postures, sides, and parks. The results were as follows: Clothing pressure according to body posture was order of `sitting-on-a-chair`, `stepping-up-a-stair`, and `standing`. High clothing pressure was obtained in the parts of calf (9.4 gf/㎠) in `standing` and knee (9.7 and 16.5 gf/㎠) in both `stepping-up-a-stair` and `sitting-on-a-chair`, respectively. The order of clothing pressure with body sides was `front`, `side`, and `back`. The highest clothing pressure was knee (18.8 gf/㎠) on the front, thigh (8.8 gf/㎠) on the side, and calf (6.4 gf/㎠) on the back. Clothing pressure of DCY at abdomen and knee was a little higher than those of SCY 3. In terms of material structure variation, clothing pressure of thigh, calf and ankle with SCY was a little higher than those with DCY, while clothing pressure of abdomen and knee with DCY was a little higher than those with SCY.

축구선수의 Detraining과 Retraining이 혈청지질 및 호르몬농도에 미치는 영향

정정화,박재현,채종훈,성혜련,황지인,윤미숙,노금선,윤종관,윤영학,노순덕,정경숙,박일규,김은희,박현태,박상갑 대한스포츠의학회 1999 대한스포츠의학회지 Vol.17 No.1

The purpose of this study was to investigate the effects of detraining and retraining on serum lipid and hormones in soccer players. Subjects were seven male high-school soccer players. V˙O_2max was determined for each subjects by administering a treadmill test(initial speed: 90m/min, grade: 5%, increasing speed per 3 min: 30m/min). Serum lipid(T-C, TG, HDL-C LDL-C) and hormones(epinephrine, norepinephrine, growth hormones, cortisol) were assayed pre and post detraining in 10, 20, 30 days after retraining. The repeated ANOVA was used to determine significant differences. The 0.05 level of significance was as critical level for the study. The results of the study were as follows: 1. V˙O_2max(ml/min) were 3576.3±204.2ml/min pre detraining, 3234.1±198.9 ml/min post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/min) were 3601.4±170.9 ml/min. There were significantly(p<.05) increased in retraining periods. 2. V˙O_2max(ml/kg/min) were significantly(p<.05) decreased from 62.3±2.9 ml/kg/min to 55.9±4.7 ml/kg/min in detraining. In 10, 20, 30 days after retraining, V˙O_2max(ml/kg/min) were 62.4±3.4ml/kg/min, 62.7±2.3ml.kg/min, 67.3±7.2ml/kg/min respectively. There were significantly(p<.05) increased in retraining periods. 3. T-C were significantly (p<.05) increased from 166.6±8.5mg/dl to 175.3±10.3 mg/dl in detraining. In 10, 20, 30 days after retraining, T-C were 160.1± 3.2mg/dl, 156.7±3.7mg/dl, 140.3±9.0mg/dl. There were significantly(p<.05) decreased in retraining periods. 4. HDL-C were 61.4±6.6mg/di pre detraining, 5.3±6.6mg/dl post detraining. There are significant(p<.05) difference between pre and post detraining. In 10, 20, 30 days after retraining, HDL-C were 56.9±7.1mg/dl, 56.4±9.2mg/dl, 57.7±9.1mg/dl respectively. There were no significant difference in retraining periods. 5. The hormones(epinephrine. norepinephrine, growth hormone, cortisol) were changed as same patterns. Epinephrine were 26.0±7.0[g/ml pre detraining, 24.6±3.2pg/ml post detraining. In 10, 20, 30 days after retraining, epinephrine were 26.9±5.6pg/ml, 30.6±6.2pg/ml, 29.4±5.6pg/ml respectively. There were no significant difference in retraining periods. In conclusion, HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were decreased, T-C, LDL-C and TG were increased in detraining. But HDL-C, epinephrine, norepinephrine, growth hormone and cortisol were increased, T-C, LDL-C and TG were decreased in retraining.

Antiadhesive effect and safety of oxidized regenerated cellulose after thyroidectomy

Kyoung Sik Park,Kyu Eun Lee,Do Hoon Ku,Su-Jin Kim,Won Seo Park2,Hoon Yub Kim3,,Mi Ra Kwon1,Yeo-Kyu Youn1 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.6

Purpose: To evaluate the antiadhesive effects and safety of an oxidized regenerated cellulose (Interceed) after thyroidectomy. Methods: Seventy-six thyroidectomized patients were prospectively randomized into two groups with regard to the use of Interceed. We evaluated each group for their adhesive symptoms using four subjective and four objective items at the 2nd week, 3rd and 6th month after thyroidectomy. All patients were examined for vocal cord motility by indirect laryngoscope at each period. Results: Total adhesion scores at each postoperative follow-up period decreased with time, but were not significantly different in each group. The median score for swallowing discomfort for liquid was significantly lower in the Interceed group than in the control group 2 weeks after surgery. In addition, the severity of skin adhesion to the trachea was reduced in the Interceed group compared with the control group 6 months after surgery. During the study, there were no adverse effects or significant differences in postoperative complications between the groups. Conclusion: Interceed appeared to be safe and effective in improving neck discomfort at early postoperative periods and preventing skin adhesion to the trachea 6 months after thyroidectomy.

지역냉방 시스템용 초고효율 판형 열교환기 개발에 관한 연구

박재홍(Jae-Hong Park),임혁(Hyug-Lim),조성열(Sung-Youl Cho),강인성(In-Sung Kang),김정규(Jung-Kyu Kim),허인은(In-Eun Hur),고성규(Seong-Kyu Ko),곽승식(Sung-Sik Kwak),김종재(Jong-Jae Kim) 한국마린엔지니어링학회 2010 한국마린엔지니어링학회 학술대회 논문집 Vol.2010 No.10

Plate heat exchanger consists of thin, rectangular, pressed sheet metal plates that are sandwiched between full peripheral gaskets and clamped together in a frame. The frame has a fixed end-cover plate, fitted with the connecting ports, which is bolted together with a movable cover plate to hold the embossed plates in between; the top and bottom carrying bars allow proper alignment of the plate stacks. When compared with the well-established shell and tube heat exchangers, the plate heat exchanger shows a lot of advantages like high NTU values, compactness, low cost, multi duties and reduced fouling etc. Plate heat exchangers are often used to transfer heating or cooling water which is produced in the energy production( district heating or district cooling) facilities to residential areas and industrial parks. The district cooling systems of Middle East are the biggest market of plate heat exchanger, but the technical specifications for cooling system are different from the domestic one. To achieve its specifications, the new plate heat exchanger was developed, and its thermal performance results are introduced in this paper.

우심방을 침범한 거대 비호지킨씨 림프종 1예

강상범,진승원,이은경,박용현,최용호,김용주,박준철,김재형,홍순조,최규보,권종범,원용순,박건,이은희 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.4

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

국립공원 내 공중화장실에 대한 탐방객 인식에 관한 연구 : 지리산국립공원을 중심으로

이호(Ho Lee),이승록(Seung-Rok Lee),박기현(Ki-Hyun Park),박은희(Eun-Hee Park),차진열(Jin-Yeol Cha),심규원(Kyu-Won Sim),강동원(Dong-Won Gang) 국립공원연구원 2011 국립공원연구지 Vol.2 No.1

본 연구는 공원 내 공중화장실에 대한 탐방객의 인식이 만족도에 미치는 영향을 규명하고자 수행하였다. 현지 설문조사는 지리산국립공원의 성삼재와 중산리 일원을 방문한 탐방객 292명을 대상으로 이루어졌다. 분석결과, 공중화장실의 관리상태, 외관, 디자인 요인 모두 탐방객의 공중화장실 이용 만족에 영향을 주는 것으로 분석되었으며, 그 중 관리상태가 가장 큰 영향을 주는 것으로 나타났다. 따라서 탐방객의 인식과 만족을 향상시키기 위해서는 공중화장실의 청결한 관리 및 유지, 악취 방지, 소모품의 원활한 공급 등에 우선순위를 두어 집중적으로 관리할 필요가 있는 것으로 나타났다. This study conducts to investigate the relationship between visitors’ perception and satisfaction. Data of 292 visitors were collected from Seongsamjae and Jungsanri in Jirisan National Park. The results showed that maintenance, outlook and design of public toilet are positively related to visitors’ satisfaction. One of the major research findings is that the maintenance of public toilet most affects visitors’ satisfaction. Therefore, the park managers need to put great emphasis on the cleanliness and maintenance, preventing stinks, consumable supplies of public toilet in order to enhance visitors’ perception and satisfaction.

螢光燈의 磁氣場에 의한 影響調査

朴德圭,金銀伶,琴慶浩 慶北大學校 1991 論文集 Vol.51 No.-

In this study, it was observed that the discharge voltage is increased(Vd ∝ H), the discharge current and luminous intensity are decreased(L ∝ H^2) as the magnetic field intensity(H) applied on the several commercial fluorescent lamps is increased. Magnetic insulation is appeared in the condition that critical transverse magnetic field is applied on the fluorescent lamps. At that condition, discharge current is reduced very low level, and then glow is disappeared. As the length of the lamp tubes is decreased, magnetic field intensity has to increased in order to occur "magnetic insulation". The experimental relation between critical magnetic field intensity for magnetic insulation anil the length of the lamp tubes (H^*d_eff/V_d^(1/2) = A) is obtained.

한국 초파리 自然集團의 성적도태에 관한 유전학적 연구

朴殷圭 圓光大學校 1986 論文集 Vol.20 No.2

本 實驗에서는 한국 自然集團에서 採集한 D. melanogaster와 突然變異型의 第1染色體에 보유되어 있는 몇 개의 遺傳子의 頻度를 파악하여 性的淘汰에 대한 集團의 遺傳的 構造를 밝히고자 하였다. 本 實驗에서는 集團飼育箱子內에서 伴性遺傳 突然變異型 초파리 3個의 系統이 野生型 초파리와 競爭하면서 살아가는 行動를 硏究조사하였는데, 本 實驗에 選擇된 突然變異型 系統은 白色眼 초파리, 黃色眼 초파리 그리고 黃色體 및 白色眼 초파리였다. 이 實驗의 일부는 45世代동안이나 계속 되었었다. 相異한 集團에서 相異한 比率로 나타나기는 했지만, 모든 경우에서 突然變異型의 頻度가 減退하고 野生型의 頻度가 增加하였다. 本人은 그 比率을 결정하는 것은 수컷이 配偶者를 획득할 수 있는 能力의 차이에 의해서 說明할 수 있고 암컷의 生存能力이나 生産能力과 같은 다른 차이는 초파리에서는 비교적 重要한 요인이 아니다는 結論을 내릴 수 있었다. 한국 自然集團에서 採集한 野生型 30쌍과 突然變異型 白色眼초파리 30상, 黃色體 초파리 30상, 黃色體 및 白色眼 초파리 30쌍으로 各集團飼育箱子에 혼입하여 계속 飼育하였다. 이대 그들 초파리 사이에서 生存價는 같았지만 黃色體 암컷 초파리는 3~9世代부터, 그 同一種 수컷 초파리 (y/??)는 10~21世代 이후로 各 集團飼育箱子에서 除去되었으며, 黃色體 및 白色眼 암컷 초파리도 2~4世代부터 그 同一種 수컷 초파리(yw/??)역시 10~21世代부터 各 集團飼育箱子에서 한 마리도 남지 않았다. 이 암컷 초파리가 突然變異型 초파리의 效率이 낮았기 때문이며, 모든 種類의 암컷 초파리가 突然變異型 수컷 초파리를 ?待한 결과인 것이다. 특히 本 實驗의 結果는 黃色體 및 白色眼 암컷 초파리가 그 同一種 수컷 초파리를 ?待한다는 사실을 입증하고 있다. 한편 白色眼 초파리도 41~42世代부터 ?里와 木浦 集團飼育箱子에서 제거되었다. 따라서 한국 自然集團의 초파리가 미국 自然集團의 초파리보다 性的淘汰에 관한 遺傳的 效率이 높다는 結果를 觀察할 수가 있었으며, 특히 한국 自然集團에서는 초파리 보다 性的淘汰에 관한 遺傳的 效率이 높다는 결과를 觀察할 수가 있었다. 이러한 淘汰現象이야말로 덜 適應한 초파리로 하여금 실로 짧은 기간에 淘汰도리 수 있게 하는 强力한 性的淘汰를 보여준 한 例로 看做된다. The present experiments were carried out to understand the genetic structure of the natural populations by means of the frequencies of some genes on the first chromosomes of the mutants and D. melanogaster collected in korean natural populations for the sexual selection experiment. The present experiments were carried out to study the behavior, in the population cages, of three sex-linked mutants of D. melanogaster in competition with wild type flies. These mutants were white-eyed flies, yellow-bodies flies, and yellow-bodied and white-eyed flies. Some of these experiments were continued for as many as 46 generations. In all cases the frequency of the mutant forms declined and the wild type increased, though at different rates in different populations. I conclude that the rates can be accounted for by the differences in the ability of the males to acquire mates, and that other differences, such as viability and fecundity of the females, are relatively unimportant in this material. In each population cage, I set up a culture of D. melanogaster containing 30 pairs of wild type flies collected in Korean natural populations, 30 paris of white-eyed flies, 30 pairs of yellow-bodied flies and 30 pairs of yellow-bodied and white-eyed flies. Although they are equal with respect to surval, after 3 to 9 generations there was not a yellow-bodied female fly to be found in the culture. The same male fly (y/??)is also eliminated in each population cage in about 10 to 21 generations. After 2 to 4 generations, there was not a yellow-bodied and white-eyed female fly to be found in the culture. The same male fly(yw/??) is eliminated in each population cage in about 10 to 21 generations. This is due to the lower efficiency of mutant flies in mating. all the females discriminate against mutant males. In the present experiments, I noted that yellow-bodied and white-eyed female flies(yw/yw?) discriminate especially against the same male flies(yw/??). The white-eyed fly is also eliminated in Iri and Mok-Po population cages in about 41 to 42 generations. Hence, the flies of Korean natural populations of D. melanogaster were showing much higher heritability for sexual selection than the flies of American natural populations. in Korean natural populations of D. melanogaster, the flies of Sang-Ju and Mok-Po populations were showing much higher beritability for sexual selection than the flies of Mt. Ueol-Chul, Mt. Gaya and Iri populations. It seems reasonable to assume that such sexual selection was strong enough to eliminate the less "fit" flies in a remarkably short span of time.