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( Young Su Joo ),( Chan Ho Kim ),( Youn Kyung Kee ),( Chang Yun Yoon ),( Eun Young Lee ),( In Mee Han ),( Seung Gyu Han ),( Jung Tak Park ),( Seung Hyek Han ),( Tae Hyun Yoo ),( Shin Wook Kang ),( Hyu 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Mean platelet volume (MPV) is suggested as an index of infi ammation and disease activity in addition to refi ecting the effi cacy of anti-infi ammatory treatment in chronic infi ammatory disorder patients. However, the prognostic value of MPV on mortality remains unclear in patients with severe sepsis. Methods: We prospectively enrolled 345 patients admitted to the emergency department (ED) who received standardized resuscitation for severe sepsis and/or septic shock between Nov. 2007 and Dec. 2011. The change in MPV between hospital admission and 72 hours after treatment (ΔMPV72h-adm) was evaluated as a prognostic factor for 28- day mortality. Linear mixed model and Cox proportional hazards analysis were used. Results: The mean age of the enrolled patients was 64.2±15.7 years, 169 (49.0%) of them were males. Thirty-fi ve (10.1%) patients died within 28 days after ED admission. During the fi rst 72 hrs of ED admission, MPV signifi cantly increased in both non-survivors (P = 0.001) and survivors (P < 0.001) compared to baseline. ΔMPV72h-adm was signifi cantly higher, indicating an increase in MPV during the fi rst 72 hours, in non-survivors compared to survivors (P = 0.003). However, the change in the number of platelets over the fi rst 72 hours did not differ signifi cantly between the two groups (P = 0.360). Multivariate analysis revealed that ΔMPV72h-adm was an independent predictor of 28-day mortality, even after adjusting for age, sex, body mass index, Sequential Organ Failure Assessment score, renal replacement therapy, platelet count, C-reactive protein level, albumin level, and lactate level (hazard ratio, 1.44; 95% confi dence interval, 1.01-2.06; P = 0.044). Conclusions: An increase in MPV during the fi rst 72 hours of hospitalization could be an independent risk factor for adverse clinical outcomes in severe sepsis.
Koh, Hung Sun,Jang, Kyung Hee,Han, Eui Dong,Jo, Jae Eun,Jeong, Seon Ki,Ham, Eui Jeong,Lee, Jong Hyek,Kim, Kwang Seon,In, Seong Teek,Kweon, Gu Hee The Korean Society for Integrative Biology 2012 Animal cells and systems Vol.16 No.5
To reexamine taxonomic status of endemic Mogera wogura coreana from Korea, we first obtained partial 12S rRNA sequences (893 bp) and complete cytochrome b gene sequences (1140 bp) of this subspecies, and these sequences and partial cytochrome b sequences (402 bp) were compared to the corresponding haplotypes of M. wogura from East Asia, obtained from GenBank. The one of three 12S rRNA haplotypes in M. w. coreana was identical to one 12S rRNA haplotype of M. w. robusta from East Asia: 10 complete and 13 partial cytochrome b haplotypes of M. w. coreana formed a single clade with one complete and four partial cytochrome b haplotypes of M. w. robusta, respectively. We considered that M. w. coreana from Korea is an endemic subspecies with only morphological differences, although it is necessary to reexamine the subspecies status of M. w. coreana. Additionally, in the 12S rRNA and complete cytochrome b sequences, M. wogura from Japan was distinct from the two continental subspecies of M. w. coreana and M. w. robusta with average distances of 1.76 and 5.65%, respectively; insular M. wogura, with within-group distances of 2.09 and 4.38%, respectively, was also genetically more divergent than the mainland M. wogura, with within-group distances of 0.08 and 0.57%, respectively. Thus, we considered that insular M. wogura of Japan dispersed into neighboring East Asian continent, which is opposite to the traditional hypothesis on the origin of Japanese M. wogura.
Hung Sun Koh, Kyung Hee Jang, Eui Dong Han, Jae Eun Jo, Seon Ki Jeong, Eui Jeong Ham, Seong Taek In, Jong Hyek Lee, Gu Hee Kweon, Kwang Seon Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2
To identify subspecies and stocks of minke whale meats purchased from Korean markets during 2005-2007, we first obtained their complete sequences of mitochondrial DNA cytochrome b and control region sequences, and compared these sequences to the corresponding sequences of the common minke whale (Balaenoptera acutorostrata), obtained from GenBank. From analyses with partial cytochrome b sequences (383 bp) and non-coding, partial control region sequences (463 bp), Korean mink whale meats are identified as products from the North Pacific minke whale (B. a. scammoni). In addition, the sequences of the partial control region from these meats showed G at site no. 298 and G or A at site no. 463, and the meats appeared to originate from the J stock within this subspecies. Thus, because the J stock has been protected since 1986, implementation of strict regulation measures to reduce their accidental fisheries by catch seems urgent. In addition, B. a. scammoni is distinct from B. a. acutorostrata, with an average Jukes-Cantor distance of 2.21% in the complete control region sequence analysis (935 bp) and 1.31% in the complete cytochrome b gene sequence analysis; the current results support the current subspecies classification, although further sequencing analyses with nuclear genes are necessary.
원발성 담즙성 경변증 16예의 임상상 : 단일 센터 경험
박동일(Dong Il Park),고광철(Kwang Cheol Koh),이준혁(Jun Hyek Lee),최문석(Moon Seok Choi),강태욱(Tae Wook Kang),문원(Won Moon),류민규(Min Kyoo Ryu),이용욱(Yong Wook Lee),김지은(Jee Eun Kim),현재근(Jae Geun Hyun),장재권(Jae Kwon Jang) 대한내과학회 2000 대한내과학회지 Vol.59 No.6
N/A Background : Primary biliary cirrhosis (PBC) is a chronic cholestatic liver disease of unknown etiology. The disease is relatively common in western countries, but so far, only about 20 cases have been reported in Korea. Thus, the purpose of our prospective study was to evaluate the clinical characteristics of PBC in Korea. Methods : Between October 1994 and February 1999, 16 patients diagnosed as PBC at our department were enrolled in this study. We analyzed these patients for the distribution of age and sex, initial symptoms and signs, associated disorders, laboratory, endoscopic, and radiologic and histologic findings. Results : Of the 16 patients, the ratio of male to female was 1:7 and the average age was 57.5 years. Pruritus (37.5%) was the most frequent presenting symptom followed by xerostomia and xerophthalmia (12.5%), jaundice (6.3%), chronic fatigue (6.3%), melena (6.3%). Associated disorders were Sj gren's syndrome (25%), arthropathy (12.5%), Raynaud's phenomenon (6.3%) and diabetes (6.3%). Abnormalities of liver function were found frequently. Antimitochondrial antibody (100%), antinuclear antibody (31.25%) and rheumatoid factor (31.25%) were found. Long-term administration of UDCA improved both clinical and biochemical signs in most patients, however, 2 patients experienced exacerbation during UDCA therapy. Conclusions : We experienced 16 cases of PBC within a short period. It is expected that we can find these patients more frequently if we suspect this disease in patients with pruritus and unexplained obstructive pattern of liver function abnormality.(Korean. J. Med 59:634-640, 2000)
고흥선,Kyung Hee Jang,Eui Dong Han,Jae Eun Jo,Seon Ki Jeong,Eui Jeong Ham,Seong Taek In,Jong Hyek Lee,Gu Hee Kweon,Kwang Seon Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.2
To identify subspecies and stocks of minke whale meats purchased from Korean markets during 2005-2007, we first obtained their complete sequences of mitochondrial DNA cytochrome b and control region sequences, and compared these sequences to the corresponding sequences of the common minke whale (Balaenoptera acutorostrata), obtained from GenBank. From analyses with partial cytochrome b sequences (383 bp) and non-coding, partial control region sequences (463 bp), Korean mink whale meats are identified as the products from the North Pacific minke whale (B. a. scammoni). In addition, the sequences of the partial control region from these meats showed G at site no. 298 and G or A at site no. 463, and the meats appear to be originated from the J stock within this subspecies. Thus, it seems to be urgent to fulfill strict regulation measures to reduce their accidental fisheries bycatch because the J stock has been protected since 1986. Additionally, B. a. scammoni is distinct from B. a. acutorostrata, with average Jukes-Cantor distance of 2.21% in the complete control region sequence analysis (935 bp) and 1.31% distance in the complete cytochrome b gene sequence analysis, and these present results support the present subspecies classification, although further sequencing analyses with nuclear genes are necessary.
Koh, Hung-Sun,Jang, Kyung-Hee,Oh, Jang-Geun,Han, Eui-Dong,Jo, Jae-Eun,Ham, Eui-Jeong,Jeong, Seon-Ki,Lee, Jong-Hyek,Kim, Kwang-Seon,Kweon, Gu-Hee,In, Seong-Teak The Korean Society of Systematic Zoology 2012 Animal Systematics, Evolution and Diversity Vol.28 No.2
The objective of this study was to determine the degree of mitochondrial DNA (mtDNA) divergence between two subspecies of $Mustela$ $sibirica$ from Korea ($M.$ $s.$ $coreanus$ on the Korean Peninsula and ($M.$ $s.$ $quelpartis$ on Jeju Island) and to examine the taxonomic status of ($M.$ $s.$ $quelpartis$. Thus, we obtained complete sequences of mtDNA cytochrome $b$ gene (1,140 bp) from the two subspecies, and these sequences were compared to a corresponding haplotype of ($M.$ $s.$ $coreanus$, downloaded from GenBank. From this analysis, it was observed that the sequences from monogenic ($M.$ $s.$ $quelpartis$ on Jeju Island were identical to the sequences of four ($M.$ $s.$ $coreanus$from four locations across the Korean Peninsula, and that the two subspecies formed a single clade; the average nucleotide distance between the two subspecies was 0.26% (range, 0.00 to 0.53%). We found that the subspecies $quelpartis$ is not genetically distinct from the subspecies $coreanus$, and that this cytochrome $b$ sequencing result does not support the current classification, distinguishing these two subspecies by pelage color. Further systematic analyses using morphometric characters and other DNA markers are necessary to confirm the taxonomic status of ($M.$ $s.$ $quelpartis$.
간경변증과 동반된 간세포암종에서 Microsatellite Instability와 DNA 복제오류 교정유전자들의 메틸화
박정호 ( Jung Ho Park ),조성범 ( Sung Bum Cho ),이완식 ( Wan Sik Lee ),박창환 ( Chang Hwan Park ),주영은 ( Young Eun Joo ),김현수 ( Hyen Soo Kim ),최성규 ( Sung Kyu Choi ),유종선 ( Jong Sun Rew ),이재혁 ( Jae Hyek Lee ),김세종 ( S 대한소화기학회 2006 대한소화기학회지 Vol.48 No.5
목적: 최근 간세포암종 발생과정에서 DNA 복제오류 교정유전자의 관련성을 시사해 주고 있으며 그 중에서도 DNA 복구과정에서 복제오류 교정계(mismatch repair system)와 O6-methylguanine-DNA methyltransferase (MGMT)의 중요한 연관성이 있음이 제시되었다. 대상 및 방법: 2000년부터 2003년까지 간세포암종으로 수술을 시행 받아 병리조직학적으로 진단된 환자들 중에서 간경변증이 동반된 40예를 대상으로 하여 hMLH1, MGMT, hMSH3과 같은 DNA 복제오류 교정유전자의 프로모터 부위 메틸화유무와 그 빈도를 조사하고 microsatellite instability (MSI)와 면역조직화학염색을 통한 이들 유전자 산물의 발현 여부와의 상관성 및 임상 특징과 연관성을 조사하였다. 결과: 수술 후 절제된 간세포암종의 평균 크기는 4.2±2.6㎝였다. 간세포암종 병기분류에서 BCLC stage A1은 12예(30%), A2는 3예(8%), A3는 8예(20%), A4는 3예(8%), B는 8예(20%), C는 6예(15%)였고, TNM stage I은 4예, II는 24예(60%), III는 7예(18%), IVa는 5예(13%)였다. 간세포암종의 조직 분화도 등급에 따라 고분화도 8예(20%), 중분화도 19예(48%), 저분화도 13예(32%) 보였다. 수술 후 간세포암종 재발은 총 25예(63%)에서 발생하였으며, 수술 후 간세포암종 재발 또는 간기능 저하로 사망한 경우는 15예(38%)였다. hMLH1, MGMT, hMSH3 유전자의 메틸화를 보이는 경우가 동반된 간경변증에서 각각 3예(8%), 27예(68%), 28예(70%)였고, 간세포암종에서 각각 2예(5%), 29예(73%), 30예(75%)로 간경변증과 간세포암종에서 높은 빈도를 보이는 DNA 복제오류 교정유전자는 hMSH3, MGMT였고 낮은 빈도를 보이는 것은 hMLH1이었다. MSI 상태는 대부분의 증례들이 안정 MSI였으며 단지 3예(8%)에서만 MSI가 관찰되었다. MGMT, hMLH1, hMSH3 단백에 대한 면역조직화학염색결과 양성반응을 보인 경우가 6예(15%), 16예(40%), 11예(28%)였다. MGMT의 경우 유전자 메틸화에 따라 MGMT 단백 소실이 있는 경향을 보였으나 의미있는 상관관계는 보이지 않았다(p=0.10). 간세포암종의 조직 분화도, 수술 후 재발 및 예후 등 임상 특징과 유전자 메틸화와는 의미있는 상관관계를 보이지 않았다(p>0.05). 결론: 간경변증과 동반된 간세포암종 환자에서 DNA 복제오류 교정유전자 중 MGMT와 hMSH3 유전자 메틸화 빈도가 높게 나타났다. 또한 간세포암종에서 낮은 빈도의 MSI를 보였으며 DNA 복제오류 교정유전자와 MSI는 서로 상관관계가 없었다. 수술 후 재발이나 예후와 같은 임상적인 특징과 DNA 복제오류 교정유전자들의 메틸화는 의미있는 상관관계는 보이지 않았다. Backgrounds/Aim: Epigenetic silencing of DNA repair genes, O6-methylguanine-DNA methyltransferase (MGMT), hMLH1 and hMSH3, by promoter hypermethylation have been observed in various cancers. However, the relationship between hypermethylation of DNA mismatch repair genes and microsatellite instability (MSI) has not been studied in hepatocellular carcinoma (HCC) associated with cirrhosis. Methods: We investigated the methylation pattern of CpG islands of 3 genes using methylation-specific PCR (MSP) and MSI in 40 patients with paired hepatocellular carcinoma and associated cirrhosis. Results: hMSH3 and MGMT were the most methylated genes in both cirrhosis (70% and 68%, respectively) and HCC (75% and 73%, respectively). The methylation of hMLH1 was rarely found in both cirrhosis (8%) and HCC (5%). Gene promoters methylated in cirrhosis were also methylated in HCC with the exception of 9 cases found to be methylated either in cirrhosis or HCC. Of 40 cases of HCC associated with cirrhosis, three had MSI-positive phenotype in which two were MSI-low and one was MSI-high. One MSI-positive phenotype was present both in cirrhosis and in HCC, while two were only in HCC. There was no significant correlation between aberrant DNA methylation of mismatch repair genes and MSI status in HCC associated with cirrhosis. Immunohistochemical expressions of hMLH1, MGMT, and hMSH3 proteins were present in 16 (40%), 6 (15%), and 11 (28%) of 40 cases of HCC respectively. There was no significant correlaton between the aberrant DNA methylation of mismatch repair genes and clinical characteristics such as histological differentiation, postoperative recurrence and mortality. Conclusions: The methylation of MGMT and hMSH3 among DNA repair genes are frequent, but those of hMLH1 and MSI is very rare in both cirrhosis and HCC. There is no significant correlation between the methylation of DNA repair genes and clinical characteristics of HCC. (Korean J Gastroenterol 2006;48:327-336)