백서 뇌에서 전기경련 충격에 의해 발현이 조절되는 새로운 유전자

정선주,박정은,강웅구,구영진,박주배,김용식 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.5

연구목적: 이 연구는 백서 뇌에서 전기경련 충격(Electroconvulsive shock, ECS)에 의해 발현이 조절되는 새로운 유전자를 검색하고, 발달단게 및 뇌 조직에 따른 발현양상을 관찰하기 위하여 시행되었다. 방법: 생후 7일, 21일된 어린 웅성 백서에 전기경련 충격을 가한 후 mRNS differential display-PCR(DD-PCR) 기법을 시행하여 발현의 차이를 나타내는 유전자를 검색한 후 서열분석을 실시하였다. 생후 7일 및 21일된 백서의 뇌와 성년 백서의 대뇌피질, 해마를 이용한 Northern blot 분석을 시행하여 검색된 유전자의 ECS 처치 후 시간경과에 따른 유전자 발현양상을 관찰하였다. 결과: ECS 처치군과 위충격군간에 발현의 차이를 나타낸 유전자 4개를 검색할 수 있었으며, 이중 ECS에 의해 발현이 증가되는 1개의 유전자를 선정하여 서열분석을 실시한 결과 EST 서열만이 보고된 새로운 유전자임이 밝혀졌다. 이 유전자는 생후 7일된 백서의 뇌에서는 ECS 처치 후 180분에 발현양의 증가가 최고치에 도달하였으나, 생후 21일된 백서의 뇌와 성년백서의 대뇌피질 및 해마에서는 360분 경과시까지 지속적으로 발현 양이 증가하였다. 결론: 이같은 결과는 ECS에 의해 생체 내에서 유전자 발현의 조절이 이루어짐을 시사하며, 향후 이러한 유전자의 세포 내 기능에 관한 연구를 시행함으로써, ECS에 의한 생체의 반응과 치료기전에 대한 이해를 넓힐 수 있을 것으로 기대된다. 중심단어:전기경련 충격·DD-PCR·유전자·백서 뇌. Objectives: This study was performed to identify genes regulated by electroconvulsive shock (ECS) and to observe the pattern of expression of genes according to different developmental stages and brain regions. Method: ECS(130V, 0.5 sec) was given to male Sprague-Dawley rats with age of postnatal day 7 and 21(P7, P21 respectively). After screening genes regulated by ECS with mRNA diffe-rential display-PCR(DD-PCR), we selected one clone among them and observed the induction of this gene after ECS by time-dependent Northern blot analysis of rat brain of P7, P21 and adult rat cortex and hippocampus. Results: By DD-PCR method, we have identified four clones whose expression was regulated by ECS. Among them, one(CP 10-2) was proved to be a new gene by sequencing and BLAST search. Its expression was increased after ECS in P7, P21, and adult rat brain. The expression of CP 10-2 reached peak level at 180 minutes after ECS in P7 rat brain, but was further increased until 360 minutes after ECS in P21 and adult rat brain. Conclusion: In this study, a new gene was identified in rat brain which showed up-regulated expression in response to ECS. Cloning and characterization of this new gene would be helpful to elucidate the effect of ECS in rat brain. KEY WORDS:Electroconvulsive shock·Differential display-PCR·Gene·Rat brain.

마케팅환경변화와 통합커뮤니케이션 디자인(ICD)에 관한 연구

정은주,이용우 동서대학교부설연구센터 2001 연구센터논문집 Vol.4 No.1

This research is aimed to study the change in design environment caused by being digitalized, to define the new concept and sphere in digital design, to find out the direct or indirect on design by the social and cultural changes resulted in informational technology and internet, and to find out the change and response in design world being brought about it. Powerful digital forms a new world and market. Internet world, a symbol of the power, changes the area, method, and the process of design. The unity of interior decoration and appliance caused by the digitalization of living space brings about that of interior decoration and production design. On account of the change of the electronic products resulted in the informational technology, product design area has been changed from the outward appearance of the design to the software design and the interface design of users. Converted from electronic publication to multimedia design and homepage design area, virtual design also faces the same problem. This phenomenon will also change design area. Banner advertisement, as a representative of new media, appears newly. Electronic newspaper, news broadcasting, radio, and magazine through Internet unite the existing printing media and mass media such as radio, TV. As a result, their respective characteristics disappear and design areas seem to be changed into Internet broadcasting which has equivalent position and method. This change will be much faster on account of the rapid development of information technology. But impersonality and inhumanity caused by digitalization is the problem which digital society and designers should solve together.

HBA 양성환자의 혈청 Type Ⅳ Collagen 측정치 비교

윤기은,문희주,박정오 서울保健大學 1996 論文集 Vol.16 No.1

The purpose of this study was to analyze the concentration of serum type w Ⅳcollagen in HBV-DNA positive patients. The results were as follows. 1. As HBV-DNA concentration of serum grew higher, that of serum type Ⅳcollagen became lower gradually. 2. Total LDH activity had a significant relationship with concentration of serum type Ⅳcollagen up to l,000ng/mL. As HBV-DNA concentration of serum grew higher, total LDH activity became lower gradually. 3. Correlation serum type N collagen with AST(GOT) and ALT(GPT) appeared to have little relation compared to LDH. But each generally had the same change in direction. As HBV-DNA concentration of serum grew higher, those of AST and ALT were decreased drastically. These were no differences between the test group and a normal group.

뇌졸중 환자의 한방 및 보완 대체요법 이용실태

양충용,신용일,주민철,길은영 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

미기상 수치모의를 위한 다층식생모델의 식생 파라메타가 바람에 미치는 영향

오은주,이화운,정용현 한국환경과학회 2003 한국환경과학회지 Vol.12 No.6

In order to make use of the protection effect against wind by the vegetation, it examined whether it should make what vegetation form and arrangement using the 2-dimensional non-hydrostatic model. When the foliage shielding factor increases, it becomes hard to take in protection effect against wind in a residential section. When it makes height of vegetation high, it becomes hard to take in protection effect against wind with height. In the comparison in the case where vegetation high is gradually made low toward wind-stream from a vegetation, and the case of making it low gradually, although former tends to receive the protection effect against wind by the vegetation, attenuation of wind velicity becomes large. In the comparison in the case where foliage shielding factor and distribution of density of leaf are gathered gradually toward wind-stream from a vegetation. It has been understood to evaluate to height the influence that the vegetation multi-layer model by which the heat revenue and expenditure in the direction of the vegetation height is considered is used, and to characterize the vegetation group by the parameter setting.

大學敎育課程 構成에 관한 硏究 : 大學의 特殊性을 살린 敎育課程의 模型開發 Development of a Model for a Curriculum Capitalizing on the Speciality

李恩奉,鄭光,朱榮淑,李鍾淑 덕성여자대학교 고등교육연구소 1988 敎育硏究 Vol.1 No.-

The purpose of this study was to develop a curriculum model for the small, private and women's university in Seoul area. The curriculum was deeply interrelated with the missions of the university stated with the resources, the tradition and tile adopted view oil education. For the example university, the speciality web counted as follows : undergraduate education rather than research as a small sine university, integrated identity of the harmonious women as a women's university and preserving diversity as a university in Seoul area was considered. The principles for a curriculum model drawn from these consideration was as follows : 1) The university curriculum should be relevant to the ideal of the university, i. e. the whole person. 2) The university curriculum should be composed of general education, concentration, and electives. These should be distributed harmoniously. 3) The general education ie the breadth component of the curriculum, and the essential area should be prescribed by the university authority. 4) The concentration is the depth component of the curriculum. The students should be given the depth experience relevant to undergraduate level at least in one area. 5) The electives can be used fer the diverse academic and individual interest and need. 6) The curriculum should be operated with the help of the university curriculum committee. The models of the undergraduate curriculum was composed under the above principles and suggested as a conclusion.

남자 중학생의 식품 중량과 열량을 중심으로 한 영양교육 효과에 관한 연구

신혜주,김은영,배윤정,승정자 숙명여자대학교 건강·생활과학연구소 2006 生活科學硏究誌 Vol.- No.22

This study was planned to suggest countermeasure of nutrition education by investigating adolescents, dietary behavior, food weight, and acknowledgement of food calories. 210 middle school boys were selected and asked to fill out the questionnaire. The subjects were divided by nutrition education experience; 1st grade who received nutrition session and 3rd grade who divided subjects. In terms of acknowledgement of food calories subjects nutrition education experience had more recognition for calories than the other group(p<0.01). When one bowl of rice, a potato, a bowel of kimchi and a carrot were asked. However recognition for meats, fishes and eggs were not different between two groups. Among milk and dairy foods the educated group showed high recognition both calories and weight of a piece of cheese than the other(p<0.05). The educated group showed better recognition for calories and weight of tea-spoon of butter, a tea spoon of oil and a tea-spoon of mayonaise, compared to the other group(p<0.01, p<0.05, p<0.05). Also the educated group showed better recognition for calories and weight of a packet of shrimp snack than the other group. The results of this study suggest that nutrition education improved the recognition of calories and weights of foods in study subjects. Also, the number of foods recognized for their calories and weights was higher among subjects who received nutrition education. These results will influence the choice the food by adolescents. Also the nutrition education are thought to be more effective by using photographs or food models, not just by lectures on theories.

HBsAg 양성혈청에서의 HBeAg 및 anti-HBe 양성율과 ALT와의 관계 고찰

문희주,윤기은,박정오,배형준,최범열 서울보건대학 1996 서울보건대학 부설 한국보건과학연구소 논문집 Vol.3 No.1

The authors investigated HBeAg and anti-HBe in 1,000 cases(male 941, female 59) of HBsAg positive among the 225,512 blood donors in Seoul Nambu Blood Center in 1994. HBeAg and anti-HBe was detected by the method Enzyme Immunoassy. The results obtained were as follows ; 1. HBeAg and anti-HBe positive rates were detected 498 cases(49.8%) and 445 cases(44.5%) respectively among 1,000 cases HBsAg positive blood donors. 2. In HBsAg positive carriers, HBeAg positive rates were not significantly different between anti-HBe positive rates, but in sex were significantly different between HBeAg positive rates and anti-HBe positive rates. 3. HBeAg positive 498 cases were analyzed with age and it was found percentages of positive rates were 63.6% in 1st decade, 53.7% in 2nd decade, 20.6% in 3rd decade, 19.6% in 4th decade and 8.3% in over 5th decade, but anti-HBe positive 447 cases were 32.4% in 1st decade, 40.5% in 2nd decade, 69.2% in 3rd decade, 78.3% in 4th decade and 91. 7% in above 5th decade. 4. HBeAg positive rate decreased stepwise with age, while anti-HBe positive rate increased stepwise with age. 5. ALT abnormal cases in the HBeAg positive donors were higher than normal cases, but ALT normal cases in the anit-HBe positive donors were higher than abnormal cases.

供血者에서의 HBsAg 發現率과 生化學的 檢査値에 관한 硏究

문희주,윤기은,김승곤,라동진,김태전,박정오,최범열 서울保健大學 1988 論文集 Vol.8 No.1

운동신경원성 질환을 동반한 전두측두엽치매의 임상양상과 신경심리소견

박기정,정용,김은주,진주희,강수진,나덕렬 대한치매학회 2003 Dementia and Neurocognitive Disorders Vol.2 No.1

Backgrounds:Frontotemporal dementia (FTD) is rarely associated with motor neuron disease (MND). This comorbidity (FTD-MND), a subtype of FTD, results in progressive dementia and muscle weakness. Among the few available reports of series of patients, however, there have been controversies about the clinical course of FTD-MND. This study, the first report of a series of FTD-MND patients in Korea, investigated demographic and clinical features, clinical course, and neuropsychological findings of nine patients with FTD-MND. Methods:Nine FTD-MND patients (2 men and 7 women with mean age 55.6±7.2 years) were selected among 45 FTD patients who met the FTD criteria proposed by the Lund and Manchester Groups. Their clinical and neuropsychological findings were analyzed retrospectively. Results:Mean age of onset was 54.3±8.0 years. The time interval from onset to death was 27±11.3 months. Presenting symptoms were personality change, hyperphagia, nonfluent speech or motor weakness. In most cases (8/9), dementia preceded the muscle weakness;in only one patient the muscle weakness preceded the cognitive decline. Regarding the muscle weakness, bulbar symptoms occurred earlier than limb weakness in four patients;bulbar and limb weakness occurred simultaneously in another four patients;in the remaining one patient limb weakness preceded bulbar weakness. Neuropsychologic tests showed no specific patterns but diffuse dysfunction in all cognitive domains. Conclusions:Our study suggests that FTD-MND predominantly affects the bulbar muscles rather than the limb muscles in early stage and symptoms related to MND occurs later than those related to FTD. The time from diagnosis to death was 10.6±6.5 months, suggesting that FTD-MND is a rapidly progressive disease. This rapid clinical course may account for our neuropsychological findings that showed general cognitive deficits father than predominant frontal dysfunctions.