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        방사선이 흰쥐 갑상샘 소포곁세포에 미치는 영향에 대한 미세구조적 연구

        김용식,양남길,안의태,고정식,박경호,김진국,Kim, Young-Sik,Yang, Nam-Gil,Ahn, E-Tay,Ko, Jeong-Sik,Park, Kyung-Ho,Kim, Jin-Gook 한국현미경학회 1992 Applied microscopy Vol.22 No.2

        This experiment was performed to study the morphological responses of the parafollicular cells of rat following X-ray irradiation. Male rats were divided into normal and experimental groups. The head and neck region of the rat, under sodium thiopental anesthesia, was exposed to 3,000 rads or 6,000 rads of radiation in a single dose, respectively. The source was a Mitsubishi Linear Accelerator ML-4MV. The target to skin distance was 80 cm, and the dose rate was 200 rads/min. The rate of experimental groups were sacrificed on the 6th hour, 2nd and 6th day after X-ray irradiation. Pieces of the tissue taken from the thyroid gland were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1M Millonig's phosphate buffer, pH 7.3), and in 1% osmium tetroxide (0.1M Millonig's phosphate buffer, pH 7.3), and embedded in araldite mixture. The ultrathin sections stained with uranyl acetate and lead citrate were observed with JEM 100 CX-II electron microscope. The results were as follow; 1. Two types of the parafollicular cells, according to their electron densities, were found, i. e., light cells and dark cells. 2. Three types of the parafollicular cells, according to their sizes of secretory granules were found, i.e., small granule cells ($85nm{\pm}20.1;64{\sim}102nm$), medium granule cells ($120nm{\pm}26.5;77{\sim}179nm$), and large granule cells ($165nm{\pm}25.7;128{\sim}189nm$). 3. The differential ultrastructural changes of the cells according to their cell types, i.e., dark and light cell, or small, medium and large granule cells, were hardly observed in the time and dose range covered by this study. 4. The morphological changes of the parafollicular cells were not pronounced after exposure to 3,000 rads of X-ray. 5. Swollen cisternae of the granular endoplasmic reticulum and partial cytolysis were observed after exposure to 6,000 rads of X-ray. 6. Above results suggest that the parafollicular cells showed the alterations of mitochondrial and granular endoplasmic reticular swelling, and partial cytolysis, but only in doses of 6,000 rads.

      • KCI등재

        골부초와 자갈대에 의한 고랭지 감자재배지 토양보전 효과

        박철수,정영상,주진호,이원정,양재의 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

        강원도 고령지 지역의 밭은 경사지에 발달한 곳이 많아서 강우에 의한 유거수와 토양의 유실이 많이 일어나게 된다. 일부 지역에서는 석비레를 이용하여 농업을 하고 있어 강우침식을 쉽게 받을 수 있다. 이러한 농경지 밭에 쉽게 적용하여 토양유실을 저감시킬 수 있도록 하기 위해 농경지 인근에서 쉽게 구할 수 있는 잡초와 토양침식 후 표면에 남은 자갈을 이용하여 골에 일정 간격으로 두어 그 효과를 살펴보았다. 토양 유실량은 식양토이면서 13%의 경사를 가진 포장에서는 사경과 등고선 경작만으로도 토양 유실량을 현저히 줄일 수 있는 것으로 나타났고, 경사가 23%,인 석비레 사양토 포장에서는 토양 유실이 심하게 발생하는 것으로 나타났다. 부초와 자갈을 최소로 이용하는 골최소부초와 골자갈대를 시험구에 처리함으로써 처리하지 않은 구에 비해 횡계와 용산 포장에서 45% 이상의 토양 유실 저감 효과와 유거수 발생 또한 저감시킬 수 있는 것으로 나타났으나, 사양토인 석비레 성토 지역의 경우는 토양 유실량이 보전처리구에서 61 Mg/ha 이상이 발생하여 골최소부초와 골자갈대를 3 m 간격으로 처리 하여서는 그 효과를 허용 토양 유실량인 11 Mg/ha 이하로 낮출 수 없는 것으로 평가되어 단일한 방법으로는 토양보전에 부족할 것으로 평가되었다. To develop proper soil management practices for reducing soil erosion, experiments were carried out by using lysimeters in Pyeongchang highland, Korea. Lysimeters installed at Hoenggye had 13% slope, 15 m slope length and 3 m width. Lysimeters with 23% slope, 15 m slope length and 5 m width were also installed at Yongsan. Soil textures in Hoenggye and Yongsan lysimeter plots were silty clay loam and sandy loam, respectively. In the lysimeters potato was cultivated, and slant furrow culture and contour culture were applied. Up-down furrow and continuous fallow lysimeter was included in the experiments as a control plot. For the slant furrow and contour culture methods, minimum furrow mulching and gravel barrier were placed at each end of the furrows in the lysimeters from April to October in 2000 and 2001 to prevent soil and nutrient losses. In Heonggye, in two years experiments, average soil loss of 17 Mg/ha was found in the up-down and continuous fallow lysimeter and 2.6 Mg/ha from furrow minimum straw and slant furrow treatment, and 1.8 Mg/ha from slant furrow and gravel bag treatment. In the contour culture, the soil losses were further reduced. In Yongsan, soil loss in the slant furrow culture without any protection treatment was 167 Mg/ha, and the soil loss was reduce to 61 and 86 Mg/ha with minimum straw and gravel bag treatments, respectively. The soil loss could be reduced more than 45% by furrow minimum straw and gravel barrier. The furrow minimum straw or gravel bag barrier successfully reduced soil loss in clay loam soil in Heonggye, but still the treatments were not enough to reduce soil loss in saprolite piled sandy loam soil in Yongsan.

      • KCI등재후보
      • KCI등재

        급성기 주요우울장애 환자에서 Natural Killer T 세포

        박이진,이제훈,이권행,한상익,전양환 大韓神經精神醫學會 2006 신경정신의학 Vol.45 No.3

        Objectives : To evaluate an association between depression and altered immunity, we examined peripheral T lymphocyte or natural killer (NK) cell measures plasma ACTH and cortisol using the flow cytometry in acute and unmedicated patients with major depressive disorder (MDD). Methods : Forty-two patients with MDD from the outpatient clinic and forty normal controls from the hospital staff were recruited. We applied Hamilton Rating Scale for Depression (HAM-D) and Hamilton Rating Scale for Anxiety (HAM-A) for depressed subjects. Peripheral T lymphocyte or NK cell measures (CD3, CD4, CD8, or CD56) and plasma hormones (ACTH and cortisol) were obtained from all subjects. Results : There were no statistical differences in CD3, CD4, CD8, or CD56 between the two subjects. The number of CD56 cells negatively correlated with HAM-D scores (r= -0.42, P<0.01), but did not correlate with HAM-A scores in patients with MDD. The number of CD56 cells showed strong negative correlation with CD4/CD8 (r= -0.47, P<0.01) in the control group, but not in the depressed group. Patients with MDD had higher cortisol level than controls within the normal range. Conclusion : The trait of immunological imbalance and HPA axis abnormality were shown in patients with MDD. Especially, the severity of depression, but not the anxiety, could be reflected as decreased number of CD56 (NK T) cells in acute and Unmedicated state.

      • KCI등재후보

        정신분열병 환자에서 세가지 청각 자극 "Oddball" 모형에 의한 사건관련전위 P3a와 P3b

        진용탁,박이진,남지민,한상익,전양환 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.5

        Objectives : Using 3-stimulus auditory "oddball" paradigm reflecting fronto/central and temporo/parietal functions Simultaneously, we examined patients with schizophrenia. Methods : fifteen patients with schizophrenia from outpatient clinic and fifteen normal controls from hospital staffs were recruited for the study. To elicit P3a and P3b, 3-stimulus auditory oddball paradigm was employed. The 3-stimulus auditory oddball paradigm was composed of standard tone (1,000 Hz, 75 dB, 80%), target tone (2,000 Hz, 75 dB, 10%) and distracter (White noise, 95 dB, 10%). Results : P3a and P3b were prominent in fronto/central and temporo/parietal areas, respectively, in both schizophrenics and normal controls. The P300 amplitude in patients with schizophrenia was reduced across P3a and P3b (p<0.01). The P300latency in patients with schizophrenia was delayed across P3a and P3b (p<0.01). Conclusion : These results were consistent with frontal and temporo-parietal lobe dysfunctions in schizophrenics. The 3-stimulus auditory paradigm could be applied for patients with schizophrenia and useful for further exploration the disorder.

      • 대학생의 인터넷중독 및 스마트폰 중독 정도와 미술 치료 인식에 대한 조사 연구

        박혜원,송승윤,윤하영,이경현,이소영,이지원,진예은,최시온,허은서,황다빈,신주현,이인영 이화여자대학교 간호학회 2018 이화간호학회지 Vol.- No.52

        Purpose: Investigate the level of Internet and smart phone addiction of college students and difference of their perception on the art therapy. Method: Data was collected using 4 categories of questionnaires. Participants of this study were 383 college students who are currently attending universities located in seoul, Kyung-Ki and Incheon. The Chi-square test, One-way Analysis of Variance, Scheffé test were performed using IBM SPSS Statistics 23.0 Result: First, the study has established that the status of attending universities, grade, people who living with, age affected the level of Internet addiction of college students. In terms of the level of smart phone addiction of college students, the status of attending universities, gender, age were the affective factors. Second, there was a significant difference on the perception of the advantages of the art therapy and the level of acknowledging it, depending on the level of Internet addiction. Finally, depending on the level of smartphone addiction, there was a significant difference in the level of perception of the art therapy, expectation toward the art therapy and the helpfulness of art therapy. The more the participants are close to the addicted level, the more they want to experience the art therapy. Conclusion: These results suggest. First, it is necessary to use bigger group of participants. Second, it is necessary to improve the research methods for college students. Third, nurse should offer holistic care toward the patients regarding their general characteristics by adapting this study. Finally, it is necessary to improve the art therapy programs for the college students who are addicted to the Internet and smartphone and to develop researches proving the effectiveness of these programs.

      • KCI등재

        고추재배를 위한 시설하우스 폐양액의 재활용

        박창진,양재의,김경희,유경열,옥용식 한국환경농학회 2005 한국환경농학회지 Vol.24 No.1

        본 연구에서는 시설하우스 폐양액이 고추의 생육과 양분 흡수에 미치는 영향을 평가하고 폐양액 관주시 부가적으로 얻을 수 있는 관개 효과를 산출함으로써 폐양액의 토양 시비에 따른 농업적 가치를 평가하였다. 시설하우스 폐양액의 토양 곽주시 Ca, Mg, K 등 염류의 증가로 인하여 pH와 EC는 상승하였고, 총질소는 암모늄태 질소와 질산태 질소의 증가와 함께 처리 후 100 mg kg^(-1) 이상 증가하였다. 고추 생육은 화학 비료 70% 및 폐양액 30% 혼합처리구와 화학비료 50% 및 폐양액 50% 혼합치리구에서 양호하였으며 양분 흡수도 동일한 경향을 나타내었다. 고추 수확량은 화학비료 70%, 및 폐양액 30% 혼합처리구에서 가장 높은 것으로 조사되었다. 1일 발생되는 폐양액(288 L 10 a^(-1)day^(-1))을 토양에 시용하여 증발산되는 수분을 보충할 경우 최고 409.86 m²의 면적을 관개할 수 있는 것으로 산출되었다. 이상의 결과를 통해 폐양액의 토양 처리는 화학비료 대체 효과와 폐양액의 정화 효과를 동시에 얻을 수 있으며 적정량 사용시 주변 환경에 미치는 영향을 최소화하면서 지속적인 처리가 가능할 것으로 판단되었다. Waste of the hydroponic solution from the plastic film house cultivation was recycled to grow the red pepper(Capsicum annum L.) in upland fields as supplement for plant nutrients and irrigation sources. Application of hydroponic waste solution increased the pH and EC of the soils, coupled with the increases in the concentrations of exchangeable cations(Ca, Mg, and K), total nitrogen, NH₄-N, and NO₃-N. Growth and yield of red pepper were highest when the treatment of chemical fertilizer(70%) was combined with hydroponic waste solution(30%). Amounts of the daily producing hydroponic waste solution were 2,880 L ha^(-1) day^(-1) from the experimental facilities and this could irrigate 409.86 m?of area to compensate for the amount of water loss by evapotranspiration(3%). The overall results demonstrated that hydroponic waste solution could be recycled as plant nutrients and irrigation water resources for enhancing soil fertility and environmental quality.

      • KCI등재후보

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