[P8-108] In vitro and Cellular Antioxidant Activities of Smilax China Extracts

Dong―Su Kim,Chong-Ik Kim,Gyo-Nam Kim,Hyun-Mi Jeong,Hae-Dong Jang 한국식품영양과학회 2007 한국식품영양과학회 학술대회발표집 Vol.2007 No.10

혈우병 소견을 가진 뇌출혈 환자 치험(治驗) 1례(例)

정인태,서병관,이현종,하지영,강미경,홍장무,백용현,박동석,최도영,Jung, In-tae,Seo, Byung-kwan,Lee, Hyun-jong,Ha, Ji-young,Kang, Mi-kyeong,Hong, Jang-moo,Baek, Yong-hyeon,Park, Dong-suk,Choi, Do-young 대한침구의학회 2003 대한침구의학회지 Vol.20 No.4

Objective : Hemophilia, a genetically determined disorder, is characterized by abnormality of the coagulation mechanism due to functional deficiency of a specific factor, namely VIII or IX. In this study, the effect of Dong-Si acupuncture therapy on the cerebral hemorrhage patient with hemophilia was evaluated. Methods : We treated him with acupuncture by stimulating Dong-Si acupuncture point(Yuk Wan) and assessed the effect by using manual dynamic evaluation of MRC(Medical Research Council) and Modified Barthel Index. Results : After treatment, the bleeding tendency was grossly reduced and bleeding lesion was absorbed gradually. And manual dynamic evaluation of the upper extremity increased from 4 grade to 5 grade. Conclusions : This result suggest that Dong-Si acupuncture therapy on the cerebral hemorrhage patient with hemophilia was effective. And futher studies are required to concretely prove the effectiveness of Dong-Si acupuncture therapy for treating hemorrhagic diseases.

가공 및 측정이 가능한 복합나노가공시스템의 개발

장동영,박만진,김진현,한동철 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

In focused-ion-beam (FIB) application of micromachining and device transplantation, four kinds of FIB processes, namely FIB sputtering, FIB-induced etching, redeposition, and FIB-induced deposition, are well utilized. As with FIB systems, scanning electron microscopes(SEMs) were extensively used in the semiconductor industry. They are the tools of choice for defect review and providing the image resolution needed for process monitoring. The enhanced capabilities of a dual-column on one chamber system are quickly becoming realized by the nano industry for performing a wide range of application.

2급 응급구조사의 성인외상환자에 대한 처치의 적절성 평가

장문수,어은경,김상현,김창섭,송근정,박한규,유지영,최동섭 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

In Korea, EMT have two classified subgroup of first and second degree : EMT-P, and EMT. For the beginning, authorization of the first EMT was made in this year through the EMT educational program. There are 8 items which EMT can perform the treatment without the supervision by a physician. But there was no previous report of pertinent assesment of treatment performed by the EMT. There upon authors have conducted review of assessment done by the EMT in EC setting, and proposed the following treatment application can be used by EMT at the field of accident. The study materials include adult trauma patient older than 15 years, and sustained trauma within 48 hours before visiting Ewha University DongDaeMoon Hospital Emergency Deparment. The duration of study was from Apr. 1 1995 to Aug. 31 1995. the study was conducted by 5 certified EMT. The EMT's performance was evaluated by the attending Emergency Medicine physician. The result were as followings ; 1. Among the total 5532 ED visiting patients during the study period, 1267 were adult trauma patients who recieved treatment within 48 hours of injury. 2. Among 1222 out of 1267 patients have completed performed survey. 3. Male to female ratio was 2.6 : 1, age distribution was from 15 to 87 years old and mean age was 34.6 years old. 4. The MOI showed : MVA (33.1%), blunt trauma (29.9%), slip down (16.8%), penetrating injury(15.4%), and miscellaneous (5.7 %), burn (3.1%), falling down injury(2.9 %), bite injury(1.1%) in order. 5. There were 437 cases of survey completed by the EMT. For assessment of mental status of 3 different method : AVPU, 5 scale mental status and GCS, were correlated with EM physician with 99.1%, 98.6%, 97.7 %, respectively. 6. For 320 out of 437 cases of survey completed, 319 patients recieved treatment by the EMT within 30 minute and 1 patient between 30 to 60 minute of an ED arrival. 7. For those 437 cases of survey completed, 266 patient (60.9%) had adequate treatment and 54 patients (12.4%) had inadequate treatment. 67 patient (15.3%) did not received treatment but it was adequate inway, and 50 patients (11.9%) did not received treatment even though patient required treatment. In cases of adult trauma patients, EMT's mental status assessment was correlated with EM physician's assessment. But treatment done by EMT without the supervision of EM physician was adequate only in 76.2 %. Therefore, continuous education and assessment of treatment performed by EMT in needed to improve field treatment by EMT future.

흰쥐의 뇌,척수 및 신경절에서 내장기관과 관련된 신경원의 추적에 관한 미세형태학적연구 - Ⅱ. 감각신경세포체와 신경섬유의 표지부위

이창현,오인균,이동협,장극선,이무삼 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.6

녹동균 세포외막 단백질 백신 CFC-1-101의 안정성 및 면역원성 검토 : 임상 제 Ⅰ/Ⅱa상 시험

장인진,김익상,유경상,임동석,김형기,신상구,장우현,박완제,이나경,정상보,안동호,조양제,안보영,이윤하,김영지,남성우,김현수 대한감염학회 1998 감염 Vol.30 No.3

목적 : 제일제당에서는 녹농균의 세포외막 단밸질을 유효성분으로 하는 백신인 CFC-101을 개발하였으며, 동물시험에서 이 백신의 안전성과 유효성을 입증하였다. 본 연구에서는 이 녹농균 백신의 인체에 대한 안전성과 면역원성을 평가하는 동시에 인체 접종시의 최적 투여 용량을 결정하기 위하여 제 I/Ⅱa상 임상시험을 수행하였다. 방법 : 건강한 성인 남자를 피험자로 선별하여 각 용량군에 백신투여자 6명, 위약투여자 2명을 배정하였다. 백신 투여군은 0.25mg, 0.5mg 또는 1.0mg 용량의 녹농균 백신을 7일 간격으로 3회에 걸쳐 근육주사 하였으며, 위약 투여군에게는 세포외막 단백질을 제외한 동일한 성분을 투여하였다. 백신접종 후 국소적 또는 전신적인 반응의 발생여부를 관찰하고, 혈액시료를 체취하여 백신의 역가와 유효성을 검정하였다. 결과 : 녹농균 백신 CFC-101은 모든 접종자에서 양호한 내약성을 보였다. 또한 0.5mg 과 1.0mg 백신 투여군에서는 100%의 항체양전율을 나타내었다. 생성된 항체는 녹농균 세포외막단백질에 특이성을 보였고, 녹농균 감염에 대해 방어효능이 있었다. 결론 : 이와같은 결과로부터 이 녹농균 백신은 인체에 안전하게 투여할 수 있으며, 높은 항체 생성능으로 감염방어 효능을 보이고 0.5mg과 1.0mg이 최적용량인 것으로 판단되었다. Background : We developed a Pseudomonas aeruginosa outer membrane protein(OMP) vaccine CFC-101, and the prophylactic efficacy of which has been demonstrated in animal models. In order to evaluate the safety and immunogenicity of the P. aeruginosa vaccine, we carried out a phase I/Ⅱa clinical trial in healthy male volunteers. Methods : Groups of eight volunteers, including two placebo subjects, were vaccinated intramuscularly with three doses of 0.25, 0.5 or 1.0 mg of the vaccine at one week intervals. Sings of systemic and local reactions observed after vaccination were recorded for each vaccinee for 5 days. Physical examinations were performed on days 0, 1, 7, 8, 14, 15, 21, and 42, and clinical laboratory tests were done on days 0, 3, and 21. Blood samples for assay of serum antibody levels were obtained up to 42 days after the first vaccination. Results : The vaccine was generally well tolerated by all vaccinees, showing no significant side effects. In the three dosage groups, all vaccinees, except one receiving the 0.25 mg dose, showed significant elevation in serum IgG antibody titers against the vaccine proteins, indicating 100% seroconversion in 0.5 and 1.0 mg groups. The human antibodies induced by the vaccine were specific for P. aeruginosa OMPs, as confirmed by western blot analysis and immunoprecipitation assays. The capacity of the human antisera to enhance opsonophagocytic killing activity by polymorphonuclear leukocytes and to confer protection against P. aeruginosa infections indicates that the antibodies elicited by the vaccine have protective efficacy. Conclusion : We conclude that the P. aeruginosa OMP vaccine is safe and effective for human use and its optimal dose to be 0.5 or 1.0 mg.

유기농산물 생산농가의 최적영농조직

장현동,김재홍 충남대학교 농업과학연구소 1997 농업과학연구 Vol.24 No.1

Producing organic farm products is one of the high-payoff farming practices considering the rapid increase of consumer's purchasing power. The purpose of this study is to identify the optimal organic farming system in relation to farm income. To cope with the above objectives, present farming conditions and profitability of 15 farmers producing organic products including rice and leaf vegetables as lettuse and cabbage were surveyed in Hongsung and Ahsan Gun, Chungnam Province. Based on the surveyed data, maximization of organic farm income were analyzed under the constrained conditions such as limited monthly labour inputs and competitive use of land by Linear Programming Model. The results of this study can be summarized as follows. 1. In the profitability analysis of 15 farmers producing organic products, rice farmers could earn their farm income more than that of conventional farmers by 50%. On the other hand, the controlled lettuce farmers could get more about 100% than that of it. But the controlled cabbage farm could get more about 40% of it. These organic farm products were saled at high prices comparing with the prices of conventional farm products by 170% ∼ 230% even though the crop yields produced by organic materials had decreased. 2. According to the labor requirement of the organically produced crops, rice cultivation was needed more labour inputs than the conventional farming method by 130%. On the other hand lettuce and cabbage could saved labour requirement by 40% and 80% of those respectively. Especially for the rice cultivation concern, higher labor requirement was due to the activities as organic fertilization, soil preparation and pest and weed controll,etc. 3. With the surveyed data from farmers who produced farm commodities, L.P. analysis was implemented to find out the optimul farming system and the maximum income. According to the results of L.P. analysis, 58% of total farm income could get more than the conventional farming system. In the case of rice cultivation, one ha of paddy was recommendable to save more labour inputs than the controlled leaf vegetables such as lettuse and cabbage. However, in the controlled leaf vegatables, only 73% of total upland equivalent to 0.3 ha should be cultivated under the condition of labor shortage as the restricted 4 workers. And increasing the size of hogs raising should be recommened to achieve self-sufficiency of organic fertilizer. As period out the possibility of organic farming, present farmers producing organic farm products could be able to maximize their income by expanding organic farm size with regard to all conditions of our organic industry. Of course, there are many difficulties in the course of developing organic agriculture. So Government should effort to support the development of our organic agriculture considering the various aspects of production, marketing, quality certification of organic produces.

Trp P-1 변이원성에 대한 Resveratrol의 항돌연변이 효과

장귀현,안병용,권용주,최동성 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.4

Trp P-1에 의해 유도된 돌연변이원성에 대한 resveratrol의 억제효과를 Salmonella typhimurium TA 98, 100를 이용한 Ames test법으로 검색하였다 먼저 틀변경 돌연변이를 일으키는 S. typhimurium TA98과 염기치환 돌연변이 특성을 지닌 S.typhimurium TA 100에서 Trp P-1의 변이원성을 비교한 결과 S. typhimurium TA98에서 복귀돌연변이의 뚜렷하게 수가 증가되었다. 이러한 결과는 Trp P-1의 돌연변이 유발은 틀변경을 주로 일으킨 돌연변이 유도란 사실을 뒷받침하는 결과로 해석되며,S. typhimurium TA98에서 Trp P-1의 최적농도는 400 ng로 나타났다. S. typhimurium TA98의 생육에 영향을 미치지 않는 농도까지 resveratrol의 농도를 assay당 2, 10, 25, 50 100, 300 ㎍으로 증가시켰을 경우 항돌연변이 효과는 각각 13%, 30%, 52%, 65%, 81% 및 89%로 나타났다. 이러한 결과로부터 Trp P-1의 변이원성에 대하여 resveratrol은 강한 돌연변이 억제효과를 나타내며, 용량 의존적으로 억제함이 확인되었다. The antimutagenic activity of resveratrol on the mutagenicity induced by Trp P-1(3-amino-1,4-dimethyl-5H-pyrido{4,3-b}indole) was studied using the Ames test with Salmonella typhimurium TA98 and 100. Trp P-1 showed strong mutagenecity in S. typhimurium TA98, but was higly decreased mutagenecity in S. typhimurium TA100. This result suggests that the mutagenecity of Trp P-1 can be mainly induced by the DNA lesions causing frame shift. Resveratrol itself did not show antibacterial effect upon 300 rig/assay. Resveratrol showed the strongest inhibitory effect with dose dependent manner on the mutagenicity induced by Trp P-1. The inhibition rates of resveratrol at concentration of 2, 10, 25, 50, 100, 300 ㎍/assay were 13%, 30%, 52%, 65%, 81%, 89%, respectively.

치료용 방사선의 입사각에 따른 광섬유 방사선량계의 체렌코프 빛 측정 및 분석

장경원,조동현,유욱재,신상훈,서정기,이봉수,박병기,문주현 제주대학교 방사선응용과학연구소 2008 방사선응용과학연구소논문집 Vol.22 No.-

In this study, we have measured scintillating light generated in a fiber-optic dosimeter by high energy photon and electron beams using a charge-coupled device. The intensity of Cerenkov lights are also measured and characterized as functions of incident angles and energies of the high energy beams from a clinical linear accelerator. To minimize or remove Cerenkov light, a simple subtraction method using a background optical fiber and wavelength discrimination method using optical filters are investigated. Also, the intensities of Cerenkov light induced by high energy photon and electron beams are compared.

불소를 포함시킨 가철성 교정장치용 Resin의 불소 방출과 굴곡강도에 관한 연구

한세현,손동수,장기택,김종철,이상훈 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.2

For the purpose of investigating the continuous fluoride releasing from acrylic resin using removable appliances by incorporating fluoride into polymethylmethacrylate during the construction of the appliances, two kinds of fluoride were blended with acrylic powder and devided into five groups as follows. Group 1: no fluoride (control) Group 2: NaF 5 wt% Group 3: NaF 10 wt% Group 4: CaF₂ 5 wt% Group 5: CaF₂10 wt% Resin specimens were tested in vitro for fluoride release with fluoride electrode and flexural strength with Instron. The results were as follows; 1. After 24 hrs, released fluoride concentration(ppm) was the most in group 3(126.5±14.15) then, next, group 2(49.5±4.92), group 5(2.67±1.08), group 4(0.81±0.30),group 1(0.03±0.01) in order, but after 28 days, group 3(3.37± 0.31), group 5(1.25±0.25), group 4(0.74±0.19), group 2(0.68±0.07), group 1(0.03±0.01). 2. Fluoride releasing of NaF gruops were faster and more amount than CaF₂groups initially but decreased significantly, later. 3. Flexural strength(㎏/㎠ ) was the biggest in group 1(602.90±69.32), and then group 4(568.98±20.16), group 5(556.19±5.26), group 2(536.12±30.35), group 3(508.44±26.16) in order, the difference between control group and NaF GROUPS were statistically significant(p<0.05).