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Trp P-1 변이원성에 대한 Resveratrol의 항돌연변이 효과
장귀현,안병용,권용주,최동성 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.4
Trp P-1에 의해 유도된 돌연변이원성에 대한 resveratrol의 억제효과를 Salmonella typhimurium TA 98, 100를 이용한 Ames test법으로 검색하였다 먼저 틀변경 돌연변이를 일으키는 S. typhimurium TA98과 염기치환 돌연변이 특성을 지닌 S.typhimurium TA 100에서 Trp P-1의 변이원성을 비교한 결과 S. typhimurium TA98에서 복귀돌연변이의 뚜렷하게 수가 증가되었다. 이러한 결과는 Trp P-1의 돌연변이 유발은 틀변경을 주로 일으킨 돌연변이 유도란 사실을 뒷받침하는 결과로 해석되며,S. typhimurium TA98에서 Trp P-1의 최적농도는 400 ng로 나타났다. S. typhimurium TA98의 생육에 영향을 미치지 않는 농도까지 resveratrol의 농도를 assay당 2, 10, 25, 50 100, 300 ㎍으로 증가시켰을 경우 항돌연변이 효과는 각각 13%, 30%, 52%, 65%, 81% 및 89%로 나타났다. 이러한 결과로부터 Trp P-1의 변이원성에 대하여 resveratrol은 강한 돌연변이 억제효과를 나타내며, 용량 의존적으로 억제함이 확인되었다. The antimutagenic activity of resveratrol on the mutagenicity induced by Trp P-1(3-amino-1,4-dimethyl-5H-pyrido{4,3-b}indole) was studied using the Ames test with Salmonella typhimurium TA98 and 100. Trp P-1 showed strong mutagenecity in S. typhimurium TA98, but was higly decreased mutagenecity in S. typhimurium TA100. This result suggests that the mutagenecity of Trp P-1 can be mainly induced by the DNA lesions causing frame shift. Resveratrol itself did not show antibacterial effect upon 300 rig/assay. Resveratrol showed the strongest inhibitory effect with dose dependent manner on the mutagenicity induced by Trp P-1. The inhibition rates of resveratrol at concentration of 2, 10, 25, 50, 100, 300 ㎍/assay were 13%, 30%, 52%, 65%, 81%, 89%, respectively.
LC-MS/MS를 이용한 농산물 중 제초제 Sulfonylurea계 36종 동시 시험법 개발
이수정,이정미,장귀현,김현경,김지영 한국환경농학회 2023 한국환경농학회지 Vol.42 No.2
Sulfonylurea herbicides are widely used in agriculture because they have a long residual period and high selectivity. An analytical method was developed using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) technique for simultaneous determination of sulfonylurea herbicide residues in agricultural products by liquid chromatography tandem mass spectrometry and for establishment MRL (Maximum Residue Limit) of those herbicides in Korea. Extraction was performed using acetonitrile containing 0.1% formic acid with MgSO4 (anhydrous magnesium sulfate) and NaCl (sodium chloride) and the extract was cleaned up using MgSO4 and C18 (octadecyl). The matrix-matched calibration curves were composed of 7 concentration levels from 0.001 to 0.25 mg/kg and their coefficients of determination (R2 ) exceeded 0.99. The recoveries of three spiking levels (LOQ, 10LOQ, 50LOQ, n=5) were in the range of 71.7-114.9% with relative standard deviations of less than 20.0% for all the five agriculture products. All validation values met criteria of the European Union SANTE/11312/2021 guidelines and Food and Drug Safety Evaluation guidelines. Therefore, the proposed analytical method was accurate, effective, and sensitive for sulfonylurea herbicide residues determination in agricultural commodities. .
Study Analysis of Isocycloseram and Its Metabolites in Agricultural Food Commodities
김지영,김효진,이수정,임수지,장귀현,문귀임,이정미 한국환경농학회 2023 한국환경농학회지 Vol.42 No.1
An accurate and easy-to-use analytical method for de-termining isocycloseram and its metabolites (SYN549431 and SYN548569) residue is necessary in various food matrixes. Additionally, this method should satisfy domes-tic and international guidelines (Ministry of Food and Drug Safety and Codex Alimentarius Commission CAC/ GL 40). Liquid Chromatography-Tandem Mass Spectro- metry (LC-MS/MS) was used to determine the isocyclo- seram and its metabolites residue in foods. To determine the residue and its metabolites, a sample was extracted with 20 mL of 0.1% formic acid in acetonitrile, 4 g magne-sium sulfate anhydrous and 1 g sodium chloride and cen-trifuged (4,700 G, 10 min, 4℃). To remove the interferences and moisture, d-SPE cartridge was performed be-fore LC-MS/MS analysis with C18 column. To verify the method, a total of five agricultural commodities (hulled rice, potato, soybean, mandarin, and red pepper) were used as a representative group. The matrix-matched cali-bration curves were confirmed with coefficients of deter-mination (R2) ≥ 0.99 at a calibration range of 0.001-0.05 mg/kg. The limits of detection and quantification were 0.003 and 0.01 mg/kg, respectively. Mean average recov-eries were 71.5-109.8% and precision was less than 10% for all five samples. In addition, inter-laboratory vali-dation testing revealed that average recovery was 75.4- 107.0% and the coefficient of variation (CV) was below 19.4%. The method is suitable for MFDS, CODEX, and EU guideline for residue analysis. Thus, this method can be useful for determining the residue in various food ma-trixes in routine analysis.