베타-글루쿠로니다제에 의한 황련ㆍ황금 공침물의 가수분해 및 생체이용률 증가

김미정,김남순,김영일,김대근,양재헌 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.2

During the preparation of decoction from the mixture of Coptidis Rhizoma and Scutellariae Radix, insoluble copreciptate was formed. The coprecipitated product(COP) was composed of berberine and baicalin which was the active ingredient of Coptidis Rhizoma and Scutellariae Radix, respectively. COP was slightly soluble in water and could not be well absorbed after oral administration. This poor bioavailibility might be associated with its poor aqueous solubility. With the purpose of increasing the solubility and bioavailibility of COP, hydrolysis of COP by β-glucuronidase was carried out. Hydrolyzed products (HOP) of COP were identified and assayed for active ingredients. The partition coefficient study, in situ absorption test, and pharmacokinetic study after oral administration were also performed. COP was found to be consisted of berberine and baicalin with molecular ratio or 1 to 1. This compound was hydrolyzed to berberine and baicalin by β-glucuronidase. The rate of hydrolysis was higher at higher temperature up to 50℃ and higher concentration of β-glucuronidase up to 2500 unit under our experimental conditions. Baicalein, which is more liphophilic than baicalin, showed greater absorption in small intestine than baicalin did. The plasma concentrations of berberine and baicalein after oral administration of HOP were significantly higher than those of COP. The possible mechanism of increased bioavailibility of berberine and baicalein could be the hydrolysis of COP by β-glucuronidase. On the basis of the above results, it might be said that HOP should be a suitable preparation for increasing the bioavailibility of Coptidis Rhizoma and Scutellariae Radix.

The level of urinary aflatoxin M1 in Korean adults

Yong-Dae Kim1, Hyojin Kwon, Sun-In Moon, Sang-Yong Eom, Jung-Duk Park, Byung-Sun Choi, Seok-Joon Sohn, Young-Seoub Hong, Ho Kim, Ho-Jang Kwon, Ji-Ae Lim, Hae-Jung Yoon, Gwang-Jin Kim, Heon Kim 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Competitive ELISA methods were used to measure the level of aflatoxin M1 (AFM1) from urine in 1008 Korean adults. Subjects were selected by random sampling in all areas of Korea, except Cheju-do. The recovery rate of AFM1 using this method was 105% (73-124%). The geometric mean of urinary AFM1 in all subjects was 3.43 pg/mL (3.67 ng/g creatinine). The level of AFM1 in males was statistically higher, compared with female subjects. However, the levels of AFM1 did not differ according to age. Subjects in Chungbuk-do showed the highest urinary AFM1 concentration, whereas subjects in Kyeongnam-do showed the lowest concentration. Assuming an excretion rate of 5%, this AFM1 excretion corresponds to approximately 0.1 microgram/day in Korean adults.

만성 간질환에 대한 DDB(Biphenyl Dimethyl Dicaboxylate)의 임상적 효과

이헌영,임의혁,김성걸,김진희,육은주,성자원,김병호,정형용,강대영,김영건 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

To investigate the effects of short-term administration of DDB(Biphenyl Dimethyl Dicarboxylate) for decreasing elevated serum aminotransferase levels in chronic liver diseases, authors applied 3 capsules of DDB per day at each meal time for 35 patients and compared with 35 patients treated with other conservative management. The following results were obtained. 1. Serum alanine aminotransferase (ALT) levels significantly decreased from 247.1± 180.0 IU/L of pretreatment level to 96.6±126.0 IU/L, 61.1±62.7 IU/L and 38.0±26.8 IU/L after 1, 3 and 6 months of treatment(p<0.05) and effective rates were observed in 60.0%, 71.4% and 88.6% of patients after 1, 3 and 6 months of treatment. 2. Serum aspartate aminotransferase (AST) levels decreased also significantly from 167.8±96.9 IU/L of pretreatment levels to 124.4±155.0 IU/L, 77.9±49.6 IU/L, 60.6± 46.9 IU/L after 1, 3 and 6 months of treatment(p<0.05), but decreasing pattern of serum AST was less significant than that of serum ALT. 3. No significant difference were observed in the serum ALT and AST changes according to the cause and duration of hepatitis. 4. No significant adverse effects were observed in whole patients treated with DDB. It is suggested that small dose administration of DDB is effective for decreasing serum aminotrans ferase levels in chronic liver disease in which other conservative management was not effective.

자궁경부에 발생한 배아성 횡문근육종 2예

박정열,조준식,김대연,이동헌,김종혁,김용만,김영탁,목정은,남주현 대한부인종양 콜포스코피학회 2002 Journal of Gynecologic Oncology Vol.13 No.3

횡문근육종은 소아와 청소년기에 가장 흔한 연부조직 육종이다. 발생 부위는 일반적으로 두부 및 경부, 그 다음으로 비뇨생식계이다 조직학적으로 배아성 횡문근육종, 국화상 육종, 폐포성 횡문근육종, 다형성 횡문근육종으로 나누어진다. 횡문근육종의 약 20%가 비뇨생식기에서 발생하고, 50% 이상이 배아성 횡문근육종이다. 여성 생식기의 배아성 횡문근육종은 드문 악성 종양으로,주로 유소아의 질에서 발생하며, 자궁경부에서 발생하는 횡문근육종은 청소년기에 흔하고, 질에서 기원하는 횡문근육종이 자궁경부에 기원하는 경우보다 5배 정도 많다. 폐경기여성에서는 자궁체부에서 가장 흔히 발생한다. 비뇨생식기의 횡문근육종의 치료는 과거에는 골반장기 적출술만으로 치료를 시도하였으나. 서서히 다중 항암화학요법, 방사선요법, 근치적 절제술을 포함한 병합요법으로 바뀌어가고 있으며, 이에 따라 생존율이 현저하게 향상되었다. 이에 저자들은 최근 본원 산부인과에서 자궁경부에 발생한 배아성 횡문근육종 2예를 경험하였기에 문헌 고찰과 아울러 보고하는 바이다. Rhabdomyosarcoma is the most common soft tissue sarcoma in childhood and young adult. Genitourinary tract is the econd most common site of rhabdomyosarcoma. Rhabdomyosarcoma can be classified into one of four major types; embryonal, alveolar, pleomorphic and undifferentiated. Embryonal rhabdomyosarcoma of the female genital tract is rare malignant tumor and usually occurs during childhood in the vagina. In rare cases, rhabdomyosarcoma can originate in the uterine cervix, with a peak incidence in the second decade. Recently we have experienced two cases of embryonal rhabdomyosarcoma of the uterine cervix. These cases are presented with a brief review of the literature.

Cefradine 함유 alginate bead의 제조 및 용출

김대섭,구자성,양재헌 우석대학교 생명과학연구소 1998 생명과학연구소 논문집 Vol.2 No.-

Cefradine, first generation cephalosporins, is a water soluble antibiotic agent. We prepared the alginate beads containing cefradine for developing release and evaluated the dissolutions of cefradine from beads. Cefradine was encapsulated into inner water phase of W/O/W emulsion beads which were prepared by cross-linking of alginate with Ca^2+. The delayed release patterns of cefradine for W/O/W emulsion beads were observed as it was compared with alginate simple beads without oil phase. As a results, W/O/W multiple emulsion beads could be use a controlled release DDS of water soluble agents such as cefradine. Also chitosan of high M.W could be a good intermediator of controlled release DDS.

자기베어링 런아웃의 적응제어

김재실,배철용,이재환,안대균,최헌오 창원대학교 산업기술연구소 2001 産技硏論文集 Vol.15 No.-

자기베어링의 회전정밀도에 영향을 미치는 인자로 PWM 전력증폭기, 위치 센서 등과 같은 자기베어링 구성 장치의 동특성 및 정밀도, 시스템의 정확한 모델링, 제어기법, 런아웃 등이 있다. 본 연구에서는 능동 자기베어링을 제어하기 위해 자기베어링의 PWM 전력증폭기와 회전축을 모델링하고 이를 바탕으로 능동 자기베어링 제어를 위한 PID 제어기를 구성하였으며, 변위 센서의 부착위치 및 회전축의 진원도의 영향으로 발생하는 주기적인 런아웃 요소를 첨가하여 런아웃의 영향을 확인하였으며, 런아웃(Runout)에 의해 발생하는 에러(Error)를 효과적으로 제어하여 자기베어링의 제어 정밀도를 향상시키기 위한 방법으로 기본적인 PID 제어기에 최소평균자승(Least Mean Square, LMS) 알고리즘을 적용한 적응 피드포워드 제어기를 구성하여 자기베어링의 능동 제어에서 발생하는 주기적인 런아웃을 효과적으로 제어할 수 있음을 MATLAB을 통한 시뮬레이션을 통해 확인하였다.

能動 振動制御 半徑方向 磁氣베어링의 强性에 관한 硏究

김재실,안승국,이재환,안대균,최헌오 창원대학교 산업기술연구소 2001 産技硏論文集 Vol.15 No.-

This article describes (1) 2 and 3 dimensional electromagnetic finite element models for an active heteropolar radial magnetic bearing, (2) the procedure for obtaining the bearing stiffnesses by simulating the models and (3) the reviews of the models by comparing an experimental test to the ideal closed loop analysis with the stiffnesses calculated from(2). The 3 dimensional model for the magnetic bearing may be very effectively applied to several types of magnetic bearings.

RT-LAMP를 이용한 콩황화일반모자이크바이러스의 진단

배대현, 이영훈, 김봉섭, 윤영남, 강범규, 최만수, 구성철, 김현태, 윤홍태, 이수헌, 백인열 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

Soybean yellow common mosaic virus (SYCMV) has been recently reported, it has been occurred a lot with Soybean mosaic virus (SMV) and Soybean yellow mottle mosaic virus (SYMMV) in soybean field. SYCMV belongs to genus of Sobemovirus and induced viral symptoms with yellowing, mottle and mosaic. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SYCML-F3/B3/FIP/BIP from coat protein gene sequence of SYCMV. After the reaction of RTLAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I. under daylight and UV light. Optimal reaction condition was at 63 for 60min and the primers of RTLAMP showed the specificity for only SYCMV tested in this study.

밤의 유통마진 및 유통체계에 관한 연구

김병택,김의경,정병헌,김대현 慶尙大學校 地域開發硏究所 1999 地域開發硏究 - 慶尙大學校 地域開發硏究所 Vol.10 No.-

이원성 기반 서브시퀸스 매칭을 위한 효율적인 방법

김상욱,박대현,이헌길 강원대학교 정보통신연구소 2002 정보통신논문지 Vol.6 No.-

In this paper, we discuss efficient processing of subsequence matching in time-series databases. We first point out the performance problems of Dual-Match, a previous subsequence matching method, and then propose a new one resolving them. The proposed method starts with a new attempt that observes the index search occurred in subsequence matching as a window-join, a kind of the spatial-join. For effective processing of the window-join, we build an R-tree for query window points within the main memory on the fly. We propose a novel algorithm for efficient processing of the join on the two R-trees; one for query window points residing in the main memory and the other for data window points residing in the disk. We also discuss the effectiveness of our approach over previous ones.