Whole-genome resequencing analysis of 20 Micro-pigs

Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.

간호사의 이미지에 따른 간호전공만족도

가미정,김수미,김영경,남수진,오루미,육지수,이혜인,최미나,최혜민,허다은 이화여자대학교 간호과학대학 2010 이화간호학회지 Vol.- No.44

Purpose: The purpose of this descriptive study is to examine the correlation between nursing students’ perception of nurses and the level of their satisfaction with the major. Method: The survey was done with the total number of 192 nursing students from 10 different universities in S city selected by a convenience samplingmethod. In order to measure the perception of nurses, the measurement which was invented by Lee(1992) and revised by Jang(1998) was used. Also, the measurement designed by Shim, and modified by Kim(2007) was used for measuring of the students’ satisfaction in their major. The collected data was analyzed by SPSS 17.0. Results: 1.The most decisive factor of the conceptualization of the image of nurses for the nursing college students is their experience of meeting nurses as a patient. 2. Students reconsider the role of nurses by experiencing immediate relationships with nurses in field training. 3. Other factors of the survey such as the students’academic year, religion, the field training situation (individual experience, type of the hospital where they experienced the training, and a relationship with the nurse in charge of the training) or the existence of nurses among their family members do not affect the students’ satisfaction. 4. There is a positive correlation between the image of nurses of nursing college students and their satisfaction with the major(0.66). Conclusion: The positive image of nurses is directly related to the students’ satisfaction in the nursing major, which hugely affects on their academic achievements and decisions of career path. Therefore, there should be more efforts to increase the positive perception of nurses among nursing students.

직업훈련 프로그램이 장애인 직업준비도에 미치는 효과

이달엽,김다혜,이승욱 한국장애인재활협회 2004 재활복지 Vol.8 No.1

직업재활을 위해서는 무엇보다 장애인의 직업적 능력을 최대한으로 개발하고 적응시키기 위한 직업훈련이 필수적이며, 이러한 훈련을 통해 직업인으로서의 바탕이 되는 자질과 기능을 충분히 갖도록 해야 한다. 직업훈령에 있어서 단순한 직업적 기능의 훈련만으로는 효과를 거둘 수 없고 직업인으로서의 전인적 훈련이 필요하다. 따라서 장애인에게 적용되고 있는 직업훈련프로그램이 직업적응, 직업유지를 위해 가장 바탕이 되는 직업준비도에 어느 정도 영향을 미치는 효과가 있는지를 파악하여 직업훈련서비스 제공에 있어서 개선을 위한 기초자료로 활용하고자 하였다. 구체적인 연구문제로 직업훈련프로그램이 장애인의 직업태도, 대인관계 및 사회기술, 직업생활 유지에 필요한 직업에 대한 인식과 직업적응에 미치는 효과와 장애인 직업준비도 요인간의 영향에 대해서 알아보았다. 본 연구에서는 WPP, VALPAR 17, 진로태도성숙도, 자기효능감 측정 도구를 사용하여 대구직업전문학교 입학생 16명을 대상으로 4-5개월 동안 직업훈련프로그램 적용 전과 적용 이후 직업인성, 대인관계 및 사회기술, 직업인식, 자기효능감을 측정하여 직업준비도의 변화정도를 파악하였다. 직업준비도에 미치는 효과를 알아보기 위해 실시한 연구를 통해 나타난 결과들은 다음과 같다. 첫째, 직업준비도 요인 변화에 있어서 직업에서 요구하는 일에 적응하는데 중요한 요인이 되는 직업인성은 직업훈련프로그램 적용 이후 향상되었고, 직업준비를 위한직업인성 형성에 효과가 있는 것으로 나타났다. 둘째, 직업훈련프로그램 적용이 장애유형에 따른 직업준비도에 미치는 영향은 직업인성과 직업인식, 자기효능감에서 장애유형에 따라 효과의 차이가 발생하였다. 셋째, 직업준비도 요인간의 상관관계는 직업인성이 높으면 자기효능감도 높아지며, 또한 자기효능감이 높을수록 직업인식이 높아지는 것으로 나타났다. 넷째, 직업준비도 요인에 대한 독립변수들의 상대적 기여도를 알아보기 위해 실시한 회귀분석 결과에서 대상자들의 나이와 장애유형, 대인관계와 사회기술이 직업인성에 큰 영향을 미치는 것으로 나타났으며, 대인관계 및 사회기술은 아니와 직업인성이 큰 영향을 미치는 것으로 나타났다. 결론적으로 직업생활 유지에 있어서 직업적응을 위한 작업준비도를 높이기 위해서는 직업인성을 향상시킬 수 있는 직업훈련프로그램이 필요하며, 이러한 직업인성을 향상시킬 수 있는 방법은 대인관계 및 사회기술 영역에 비중을 둘 수 있는 직업훈련프로그램이 필요하다. 그리고 직업훈련프로그램의 직업유지나 직업적응에 대한 효과성 조사와 훈련교사들에 대한 교육도 지속적으로 이루어져야 하며, 이러한 것들이 프로그램구성이나 교과과정 작성 시 반영하는 연구설계가 필요하다. It is essential for rehabilitating persons with disabilities, to develop employment skills. To maximize vocational potential and abilities of persons with disabilities, it is required for them to obtain work attitudes and skills. However, the work skills training alone may not be enough for persons with severe disabilities, requiring them t o have work preparation services. Therefore, this study was aimed to understand whether the vocational training program provided by Dae-Gu Metropolitan Training Institute for Persons with Disabilities had impact on the improvement of both work skills and job readiness skills for them. Data gathered included the following areas. The effect of the disabled's vocational behaviour. the personal relations, the social skills, the vocational consciousness and the job readiness areas. The WPP, VALPAR 17, Course Attitude Maturity, Self-Efficacy Feeling Measurement Tool were utilized for the data gathering purposes. For vocational Rehabilitation, first of all, it is essential for disabled trainees to develop and adapt through individualized service provisors. They are required to have enough appropriate attitudes and skills which basic to average workers. For the vocational training, a simple vocational skills training alone may not be enough. Therefore, this study was aims to figure out whether the vocational training program which have applied to the trainees in Dae-Gu Vocational Training Institute (DGVTI) was effective or not. This training program was composed of the work adaptation training and job readiness training. Initial study questions posed are as follows. Whether this training program had effect on the research participants' vocational behaviours, personal relationship skills, social skills, the vocational consciousness and the job readiness skills. The current researcher utilized the WPP, VALPAR 17, Course Attitude Maturity. Self-Efficacy Feeling Measurement Tool. They measure types of vocational personality. personal relations and social skills, vocational and self-efficacy feeling in comparison with before and after the application of training program. The 16 research participants had exposed to 4-5 months in Dae-Gu Vocational Training Institute(DGVTI), operated by Korea Employment Promotion Agency for the Disabled (KEPAD). Statistical analyses focused on the means, standard deviations, and frequencies. Futher analyses included t-tests, correlation Analyses, regression analyses. The results of this study can be summarized as follows. First, in the job readiness skills area, after training, vocational personality (which is the important factor adapting to assignment demanding occupation) is improved, and reveals that the formation of vocational personality for job readiness and, in the vocational recognition and self-efficacy feeling are positively changed. Second. job readiness skills had effect to improve research participants' vocational adaptation skills, vocational recognition and self-efficacy feeling. Third. "correlation between job readiness factors" appeared that if scores of the vocational personality is high, then the self-efficacy feeling also rises. When the self-efficacy scores are high, the vocational recognition rises. Fourth, the results of regression analyses indicated that the age and the types of disabilities of the research participants had influenced on the occupational personality. Also, research participants who showed good personal relation skills and social skills had good job readiness. It is conclusive that the method of improving occupational personality would influence on personal relations and social skills. In conclusion, it is necessary for the rehabilitation practitioners to provide skills and interpersonal skills with their clients and trainees. The implication of this study is that there is a strong need to improve quality of vocational training programs.

안내염과 대장암이 동반된 재발성 간농양

전승정,김태헌,류민선,오다연,송명은,이신아,류재인,김혜인,문일환,유권 이화여자대학교 의과학연구소 2011 EMJ (Ewha medical journal) Vol.34 No.2

The causes of pyogenic liver abscess has been known as biliary tract disease or intrabadominal infection but the large proportions of the patients has no apparent underlying disorders. Recently colonic mucosal lesions were reported in patients with cryptogenic liver abscess and it has been suggested that colonic mucosal break may play a role in developing liver abscess in otherwise healthy patients. We experienced a patient of severe recurrent liver abscess complicated with endophthalmitis only 3 months after successful treatment of initial cryptogenic liver abscess and a polypoid colon cancer was discovered by chance. It seems prudent to proceed colonoscopic examination in patients with cryptogenic liver abscess especially when it is recurrent.

Reports : Structure-activity relationships of the intramolecular disulfide bonds in coprisin, a defensin from the dung beetle

( Jae Ho Lee ),( Da Eun Lee ),( Hye Min Choi ),( Ha Hyung Kim3 ),( Ho Kim ),( Jae Sam Hwang ),( Dong Gun Lee ),( Jae Il Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.11

Defensins, which are small cationic molecules produced by organisms as part of their innate immune response, share a common structural scaffold that is stabilized by three disulfide bridges. Coprisin is a 43-amino acid defensin-like peptide from Copris tripartitus. Here, we report the intramolecular disulfide connectivity of cysteine-rich coprisin, and show that it is the same as in other insect defensins. The disulfide bond pairings of coprisin were determined by combining the enzymatic cleavage and mass analysis. We found that the loss of any single disulfide bond in coprisin eliminated all antibacterial, but not antifungal, activity. Circular dichroism (CD) analysis showed that two disulfide bonds, Cys20-Cys39 and Cys24- Cys41, stabilize coprisin’s α-helical region. Moreover, a BLAST search against UniProtKB database revealed that coprisin’s α-helical region is highly homologous to those of other insect defensins. [BMB Reports 2014; 47(11): 625-630]

Effects of dietary bee venom on growth performance, meat quality, relative organ weights, and intestinal secretory IgA concentration in broiler chickens

Da-Hye Kim,Sang-Mi Han,Yong-Suk Lee,Youn-Geun Joung,Moung-Cheul Keum,Sang Lee,Byoung-Ki An,Kyung-Woo Lee 한국양봉학회 2016 한국양봉학회 학술대회 자료집 Vol.2016 No.10

Perilla frutescens sprout extract prevented cytokine-induced β- cell damage via NF-κB pathway in RINm5F cell

Da-Hye Kim,Sang-Jun Kim,Young-Chan Jeong,Seung-Il Jeong,Kang-Yeol Yu,Chun-Jin Chon,Yoon-suk Song,Seon-Young Kim 한국식품영양과학회 2016 한국식품영양과학회 학술대회발표집 Vol.2016 No.10

Expression Phenotypic-Genotypic Associations of Tyrosinase SNP with Changing Profiles in Korean Native Chicken Plumage Pigmentation

Da-Hye Shin,Hye-Mi Park,Chan-Seong Jeong,Sang-Hwan Kim 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

SMAD4 gene mutation found in endometrial cancer patient, Myhre syndrome

( Hye Jin Kim ),( Eun Hye Cho ),( Do Hwa Im ),( Da Hyun Kim ),( Eun Jung Jung ),( Jung Mi Byun ),( Young Nam Kim ),( Kyung Bok Lee ),( Moon Su Sung ),( Dae Hoon Jeong ) 대한산부인과학회 2023 대한산부인과학회 학술대회 Vol.109 No.-

Development of air-blast dried non-<i>Saccharomyces</i> yeast starter for improving quality of Korean persimmon wine and apple cider

Kim, Da-Hye,Lee, Sae-Byuk,Jeon, Jun-Young,Park, Heui-Dong Elsevier 2019 International journal of food microbiology Vol.290 No.-

<P><B>Abstract</B></P> <P>A total of 512 yeasts, including 422 non-<I>Saccharomyces</I> yeasts, were isolated from various fruits including apple, aronia, Muscat Bailey A grapes, and persimmon. These were used to prepare persimmon wine and apple cider starters that produced high levels of aromatic compounds, which contribute to high-quality fermented products. Environmental tolerance testing with 20% glucose and 8% EtOH, alongside a sniffing test, led to the selection of <I>Wickerhamomyces anomalus</I> (Synonym <I>Pichia anomala</I>) SJ20, <I>Meyerozyma caribbica</I> (Synonym <I>Pichia caribbica</I>) YP1, <I>Pichia kluyveri</I> CD34, <I>Hanseniaspora uvarum</I> SJ69 (for persimmon wine), <I>W. anomalus</I> CS7-16 (for apple cider), and <I>Starmerella bacillaris</I> (Synonym <I>Candida zemplinina)</I> CD80 (for both wines) as wine starters. These strains had high environmental stress tolerance and the highest sniffing test scores. Persimmon wine and apple cider were fermented using these strains in single- or mixed-culture with <I>S. cerevisiae</I> W-3 to determine the improved effect on wine aroma. In accordance with the results of volatile ester compounds and sensory evaluation, <I>W. anomalus</I> SJ20, <I>H. uvarum</I> SJ69, and <I>W. anomalus</I> CS7-16 had an excellent potential as persimmon wine and apple cider starters. Moreover, other strains also showed a good potential for a distinctive persimmon wine and apple cider because of the different compositions of the various volatile ester compounds. Six types of sugars (fructose, glucose, maltose, sucrose, raffinose, sucrose, and trehalose), four types of rehydration solutions (distilled water, 1× phosphate buffered saline, 0.85% NaCl, and 1% peptone water), and two types of antioxidants (<SMALL>L</SMALL>-ascorbic acid and glutathione) were examined to improve the survival rate of air-blast dried non-<I>Saccharomyces</I> yeast cells. Optimal sugar and rehydration conditions for each strain were validated, and scanning electron microscopy showed that each cell was surrounded by protectants, including sugar, skim milk, and lactomil. Storability assessment of air-blast dried yeast cells maintained at 4 °C for two months indicated that at least one condition in each strain had a higher survival rate than the control, regardless of the concentration or type of antioxidant treatment, except for <I>M. caribbica</I> YP1. These results suggest that antioxidant treatment contributes to maintaining the viability of air-blast dried cells in hostile environments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Selecting 6 non-<I>Saccharomyces</I> yeast strains as persimmon and apple wine starters </LI> <LI> Characterization of persimmon wine and apple cider fermented using selected strains </LI> <LI> Optimizing protectant, rehydration and antioxidant conditions for yeast starters </LI> <LI> Antioxidant additives improved starters storability after 2-month of storage at 4 °C </LI> <LI> Yeast starters maintained appropriate fermentation rate for wine fermentation </LI> </UL> </P>