나프틸기를 포함하는 퓨록산 유도체의 합성

黃匡鎭,南賢祐 홍익대학교 산업기술연구소 2002 産業技術 Vol.12 No.-

Furoxan derivatives containing naphthyl group was designed and synthesised as pohtochemical nitric oxide precussor. Naphthyl furoxan 4 was prepared by the [2+3] cycloaddition of the nitrile oxide generated in situ from the iminoyl chloride 3. The preparation of iminoyl chloride 3 was proceeded from naphthyl aldehyde 1 by the reaction with N-chlorosuccinimide following oxime formation. Naphthyl furoxan 4 was irradiated on 300 nm in CH2Cl2 to give alkyne 5 confirming NO generation.

토끼에서 Etofenprox와 Spinosad 합제의 국소자극성 시험

황윤환,김명석,송인배,박병권,양승호,임용현,송치용,안성한,남현수,임정교,정상일,명노일,윤효인 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2008 動物醫科學硏究誌 Vol.15 No.1

This study was performed to evaluation the skin and eye irritation of etofenprox 8% and spinosad 4% combination in male New Zealand White rabbits. In skin irritation test, the combination of etofenprox and spinosad did not showed any change of body weight but caused slight skin irritation with the P.I.I. (primary irritation index) value 0.25. The etofenprox and spinosad combination showed severe eye irritation 1 h, 24 h, 48 h, and 72 h after ocular treatment, having the A.O.I. (acute ocular irritation) value of 37.3. Therefore, these result indicated that the combination of etofenprox and spinosad might be irritant formulation on eye and skin.

오매(Fructus Mume)의 항산화물질의 분리 및 특성연구

황현주,안은미,백남인,조재선,김해영 경희대학교 생명자원과학연구원 1998 硏究論文集 Vol.19 No.-

천연물로부터 새로운 항산화제를 개발하기 위한 연구의 일환으로 한방에서 사용되는 한약재 중의 하나인 오매(烏梅)의 물 추출물과 메탄을 추출물 및 순차 분획물에 대해 팜유, 우지와 돈지를 기질로하여 유지저아시험, Rancumat method, 전자공여작용 등으로 항산화성을 확인한 결과, EtOAc 분획물에서 강한 항산화 활성을 나타내어 column chromatography, thin layer chromatography를 통하여 활성성분에 관한 연구를 시도하였다. 1. 오매 용매분획물 중 EtOAc 분획물(400 ppm)은 유지저장실험(60℃)에서, 팜유, 우지와 돈지에 모두 우수한 효과를 나타내었으며, 특히 우지에서 높은 활성을 나타내었고, BHA(200 ppm)와 α-tocopherol(200 ppm)보다 활성이 높았다. 2. 오매 EtOAc 분획물(400 ppm)은 Rancimat method에서는 팜유, 우지와 돈지에 비교적 우수한 효과를 나타내었으며, 우지에서 AI가 4.39로 상당히 높은 활성을 나타내었다. 3. 오매의 용매분획물 중 EtOAc 분획물이 DPPH라디칼에 대한 강한 소거활성을 나타내었으며, 그 활성은 EtOAc 분획물의 농도에 의존하여 증가하였고, SC_(50)은 21.2μg/ml이었다. 4. 오매 EtOAc 분획물에서 분리한 8개 획분 중 E4에서 SC_(50)이 16.7μg/ml으로 높은 DPPH 라디칼 소거활성을 나타내었으며, 원인 물질은 여러 가지 스펙트럼 측정 결과 수종 물질이 혼합물인 것으로 밝혀져, 보다 순수한 형태로의 분리 후 정확한 동정의 시도가 요구되었다. Fructus Mume is the roasted hits of Prunus mwme and has been used as tradibonal Chmese medicine. The anboxidatwe effect of the extract from Fructus Mume was measured using different methodsn such as, electron donating abdihes(EDA), Rancimat and oven tests. The methanol extract obtained from the Fructus Mume was solvent-fractionated with ethyl acetate, n-butanol and water, respectively. The ethyl acetate fraction showed the highest antioxidative activity among them. To identify the compounds with antioxidative activity, the isolates from thm layer chromatography were studied further. This study demonstrates that Fructus Mume might be useful for the prevention or treatment of disorders associated with oxidative damage.

인터넷을 통한 연구센터 데이터베이스 시스템 구축

황의천,김일남,김남호,이봉형,왕수현 강원대학교 석재복합신소재제품연구센터 1997 석재연 논문집 Vol.2 No.-

본 연구의 목적은 석재복합 신소재제품 연구센터의 데이터베이스를 인터넷상에서 구축하여 이 데이터베이스가 연구활동 및 산업지원활동을 지원하도록 하는데 있다. 연구센터와 관련된 데이터베이스를 인터넷상의 세계정보망(world-wide web)이 연계 및 등록시켜 적극적으로 연구소의 활동을 홍보함과 동시에 세계정보망을 통한 각국의 다소 산업체의 산업정보를 실시간(real time)에 검색할 수 있는 정보망을 구축할 것이다. 석재복합 신소재제품 연구센터의 연구활동, 연구결과와 관련 산업체의 활동들을 데이터베이스화한 뒤, 이를 인터넷상에 제공함으로써 연구소의 연구활동 및 관련산업체들의 활동을 국제적으로 홍보하며 각국의 수요와 온라인으로 상담 및 전자우편을 나눌 수 있으므로 해서 지역경제의 국제화를 맞을 것으로 기대된다. 또한 앞으로 있을 정보고속도로의 구축과 활용을 위하여 본 연구는 기여하게 될 것이다. The purpose of this study is to construct the database of RRC research center on internet to support the research activities and industrial activities. Registering the database on internet, the center will proactively advertise the local industrial activities and will construct the information network which will enable the real-time search for industrial information. The database will include RRC's research activities, research output, and related industrial's activities. The homepage will advertise the RRC's activities worldwidely, support the online business, provide the e-mail, and it will enable the research center to be the leader of internationalization of local economy. The study will also contribute to further study of information highway.

니트로크라이센 유도체의 합성에 관한 연구

黃匡鎭,김진국,남현우 홍익대학교 산업기술연구소 2001 産業技術 Vol.11 No.-

In an attempt to provide a useful light-emitting material in the organic electroluminescence diode, nitrochrysene 5 was prepared by the nitration of the tetrahydrochrysene 4 using dinitrogen tetraoxide as a key step. The chrysene 4 was obtained from the methyl cinnamate in 3 steps. The mono-nitration was confirmed by the IR, ^1H-NMR and ^13C-NMR spectra of chrysene 5. The results suggest that the synthetic methods for the nitrochrysene 5 is applicable to the synthesis of various chrysene derivatives containing donor-acceptor groups at 2, 8-positions.

The expression patterns of RANKL and OPG in murine tooth eruption

Hwang, Kyung-Mun,Kim, Eun-Jung,Kim, Hyun-Jung,,Kim, Young-Jin,Nam, Soon-Hyeun 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

치아의 맹출은 치아기 (dental organ)와 치조골의 세포와 연관된 매우 복잡한 과정이다. 우선 치아 맹출이 일어나기 전에 파골세포가 치낭으로 집결하게 된다. 이러한 치낭의 역할은 파골세포와 조골세포의 상호작용으로 이루어 지는 골개조와 밀접한 관련이 있는데, 이는 치아 맹출과 연관된 많은 유전자들이 치낭에서 발현되기 때문이다. RANKL는 TNF ligand family로써 조골세포에 존재하며 파골세포의 형성 및 전구세포로 부터의 활성화를 유도한다. 이러한 RANKL은 OPG에 의해 그 작용이 억제되며 RANKL와 OPG의 상대적인 비율이 파골세포의 형성에 영향을 미친다. 또한 Runx2유전자의 변이는 조골세포의 분화와 활성에 차질을 가져오고 결국 RANKL/OPG pathway를 통해 파골세포 형성에 영향을 줄 수 있다. 치아의 발육 및 맹출에 미치는 RANKL 및 OPG의 영향을 알아보고 Runx2와의 연관성을 알아보기 위해 in situ hybridization 방법으로 태생 1, 3, 5, 7, 9, 11일된 쥐의 하악 및 제1대구치를 사용하여 실험을 실시한 결과 RANKL, OPG, Runx2의 mRNA가 태생 1일부터 11일까지 치낭 및 치아주위조직에 특성있게 나타났다. 이중 태생 5일에서 9일 사이에 RANKL 및 Runx2는 치아의 교합면측과 하방 치조골 부위의 발현이 강하게 나타난 반면 OPG는 약한 발현을 보였다. 이는 또한 파골세포의 활성부위를 알아보기 위해 TRAP염색을 실시하여 태생 5일에서 9일 사이에 최대의 활성화를 나타낸 결과와 연광성 있게 나타났다. RANKL, OPG, Runx2의 특성있는 발현양상들을 종합해 볼 때, 치아 맹출은 치낭, 치아기, 치조골 사이의 상호 작용을 통해 이루어 지며, 이는 치낭이 치아 맹출에 있어서 매우 중요하다는 것을 의미한다. 또한, 이러한 유전자들 (RANKL, OPG, Runx2) 이 치아의 맹출에 중요한 역할을 하는 것으로 사료된다. Tooth eruption is a complex and tightly regulated process that involves cells of the tooth organ and the surrounding alveolus. Osteoclast precursors must be recruited into the dental follicle prior to the onset of eruption. This function of dental follicle may be regarded as the ability of bone remodeling characterized by the interaction of osteoclasts and osteoblasts. This is because tooth eruption is a localized event in which many of the genes required for eruption are expressed in the dental follicle. RANKL is a membrane-bound protein that is a member of the TNF ligand family, which is present on bone marrow stromal cells and osteoblasts, and induces osteoclast formation and activation from precursor cell. The biologic effect of RANKL is inhibited by OPG and, in bone, the relative ratio of RANKL and OPG modulates osteoclastogenesis. To evaluate the roles of RANKL and OPG in tooth eruption and the relations with the expression pattern of Runx2, in situ hybridization was performed with mandibles of mice at postnatal stage 1, 3, 5, 7, 9 and 11. mRNA of RANKL, OPG, and Runx2 are expressed in dental follicle and surrounding tissue from P1 to 11. To determine the sites of osteoclastic activity duing tooth eruption, mandibles were dissected. Peak osteoclastic activity in alveolar bone along the occlusal and basal regions was observed from P5 to 9, with osteoclasts in these regions being large and strongly TRAP-positive. The specific spatic-temporal expression patterns of RANKL, OPG and Runx2 in our study suggest that tooth eruption could be progressed through the interactions of molecular signaling among dental follicle, dental organ and alveolar bone, furthermore it means that dental follicle is quite important in tooth eruption. In addition, it indicates that these genes (RANKL, OPG, and Runx2) play critical roles in tooth eruption.

조대술에 의한 하마종의 치료

강동균,황경문,김은정,김현정,김영진,남순현 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

하마종은 구강저에 발생하는 점액 남종으로 주로 설하선과 관련된다. 즉, 하마종은 설하선 분비도관의 파열로 인하여 타액이 분비도관에서 빠져나와 주위의 연조직 내에 저류됨으로써 발생하는 가성 낭으로 정의 되며, 원인은 설하선 도관의 파열이나 폐색이다. 하마종의 임상소견은 청색의 투명한 종창이 구강저의 소대를 중심으로 편측으로 발생하며, 무통성이며, 촉진시 파동성을 나타낸다. 조직소견은 결합 조직 내 와동이 형성되지만, 낭 벽은 상피세포가 아닌 육아조직으로 구성되는 가성 낭을 관찰할 수 있다. 하마종의 첫 번째 치료법으로는 하마종의 내벽이 구강점막의 일부가 되도록 만들어 주는 조대술을 고려할 수 있다. 본 증례는 특징적인 하마종의 임상소견을 보이는 환아에게 gauze-packing등을 동반한 조대술을 이용하여 치료한 증례로써 양호한 치료결과를 보여 보고하고자 한다. Ranula is a mucocele which occurs at the mouth floor and mostly related to sublingual gland. In other words ranula is definded as a pseudocyst which occurs as the secretion duct of sublingual gland is destructed there as the saliva from the the secretion duct flows out and retention in the soft tissue. The cause of ranula is destruction or obstruction of the duct. The clinical findings of ranula is a painless, unilateral bluish transparent swelling around the frenum and shows fluctuation when palpated. Histological finding represent a formation of cavity inside the connective tissue. but a pseudo-cyst can be seen which the wall of the cyst is composed of granulation tissue rather than epithelial cells. The first treatment of ranula can be considered as marsupialization. which induces the inner wall of the Ranula to be a part of oral mucosa. This case report shows a treatment of marsupialization with gauze packing in a young patient representing a clinical finding of characteristic ranula.

활성 알루미나의 제조방법에 관한 연구

남영우,김현철,황희연 崇實大學校 生産技術硏究所 1994 論文集 Vol.24 No.-

Aluminum hydroxide gels were formed from aluminum salts by precipitation method. These gels were aged and calcined to get γ- alumina. The effects of aging condition for aluminum hydroxide gels on the physical properties of γ- alumina were tested varying aging pH, time and temperature using aluminum sulfate as starting materials. The effect of starting materials were tested using aluminum sulfate, nitrate and chloride salts. Activated alumina prepared from Boehmite aged under the condition of pH 11 and 170℃ showed high surface area and large pore volume. Aging time above 1 day showed a little effect on the properties of activated alumina. Optimum aluminum salt concentration for high surface area and large pore volume was 4 wt%.

알레르기성 소인이 없는 정상인에서 특이 알레르겐항체 양성율

남해선,안현철,박준수,황규윤,박춘식 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

This study was designed to evaluate prevalence of allergen-specific antibodies in healthy adults. During Mar. ~ Aug. 2001, 1,925 subjects visited for medical check-up, were inquired to fill out a standardized questionnaire. Among them, 200 subjects who had no current and past allergic disorders were randomly selected for rhe current study and examined for allergen-specific antibodies using Korean inhalant panel of MAST-CLA allergen-specific IgE assay. Among them, 170 persons (85.0%) did not have any positive allergen-specific antibody. Each positive rate of 35 allergen specific antibodies was usually less than 3%, except Cockroach Mix (3.5%), Dermatophagoides farinae(8.5%), Dermatophagoides pteronyssinus(6.0%). Among 30 positive subjects, 19(63.3%) showed one or two allergen-specific antibodies. Although atopic subjects in non-allergic population were not common, few of them showed allergen-specific antibodies in Korean inhalant panel of MAST-CLA. Our results suggested that the standardized questionnaire and MAST-CLA could be an useful tool of a screening test for allergy, showing high specificity and sensitivity.

순천향대학 천안병원에 내원한 알레르기환자의 특성

황규윤,남해선,,안현철,박준수 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.1

This study was designed to evaluate gencral features related to allergy of allergic patients living in northern and western Chungchungnamdo, visited to an allergic unit in Soonchunhyang University Chunan Hospital during August 1998 to July 2000. Three hundred allergic patients were enrolled. Among them, 87.7% were outpatients and were analysed for the study. Demographic variables as well as resident living conditions and immunologic results were assessed. Overall IgE, house dust, Dermatophagoides farinae (Df), and Dermatophagoides pteronyssinus (Dp) antibody were assessed using MAST-CLA in 263 outpatients. Relations between allergens and age, sex, and living conditions were evaluated by chi-square tests. Mean age was 21.0 ± 17.3 year-old and male accounted for 59.3% in total subjects. Among them, 52.0% lived in an apartment and 65.3% were from urban area near Chunan and Asan city. Admission rate of allergic patients was 12.3%. The positive rates of total IgE, house dust, Df, and Dp were in 55.1%. 19.0%, 30.0%, and 29.3% in outpatients, respectively. No significant relations between allergens and sex. living home, and region were observed (P>0.05). Adolescents showed significantly higher positive rate of total IgE (P=0.02). Our data suggested that residential environment may not be a significant factor of main causative allergens in northern and western Chungchungnamdo. However more controlled study should be required to elucidate the relations between living environments and major causative allergens in this area.