조사연구에서 표본설계에 대한 고찰

양철호,양선화,신봉관,조지현 동신대학교 2005 論文集 Vol.15 No.-

Most of the survey research are conducted with samples. Thus, representativeness of the samples is crucial for the sound research. Reports of the various survey research were analized. The results of the analysis showed that different ways of sampling methods were utilized in various studies in order to ensure the selection of unbiased samples under the circumstances of purpose and population of the survey.

혈액관리학과 교육과정 개발

조갑철,최양자,신기수,손선영 적십자간호전문대학 2000 적십자간호전문대학논문집 Vol.22 No.-

급성충소돌기염의 임상 : 병리학적 고찰

양기화,조준필,하영천,한진우,정학재,손수창,이성희,황재훈,신철호 최신의학사 1989 最新醫學 Vol.32 No.4

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

인슐린 비의존형 당뇨병 환자에서 혈장 Endothelin-1농도의 변화

신양수,조희충,김원식,국기용,김용화,정종훈,문철웅,배학연,양성훈 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

Endothelin(ET) is a 21-residue peptide originally isolated from the cultured porcine endothelial cells. There are at least three genes for endothelin:endothelin-1(ET-1), endothelin-2(ET-2), and endothelin-3(ET-3). Endothelins are present in various human biological fluids including plasma, urine, breast milk, and saliva and have been found elevated plasma ET concentrations in patients with diabetes mellitus, Patients undergoing maintenance hemodialysis due to chronic renal failure, patients with acute myocardial infarction, and patients with subarachnoid hemorrhage. Endothelial cell damage is suspected to occur in diabetic patients and may be one important cause of angiopathy, a major complication in diabetes mellitus. The elevation of ET in diabetic patients may be a marker of, and further exacerbate, their vascular disease. We measured the levels of ET-1 in plasma of 50 patients with non-insulin dependent diabetes mellitus(NIDDM) and 25 normal subjects by radioimmunoassay. The plasma ET-1 concentration (mean±S.D.) in NIDDM was 6.461 A2.510 f㏖/ ㎖, and was significantly higher than in normal subjects (4.567±1.155f㏖/㎖) (P<0.05). The plasma ET-1 concentration (mean±S.D.) in diabetic retinopathy group( 7.15±2.454 f㏖/ml) was significantly elevated than those in otherwise uncomplicated groups (5.348±2.390 f㏖/㎖)(P<0.01). The correlation between any clinical parameters and plasma ET-1 levels in NIDDM was not significant, In conclusion, this study suggest that the elevated levels of ET-1 in diabetic patients may be play a important role in the pathogenesis of diabetic complications.

비소세포 폐암종에서 Cyclin D1, p21 및 p53단백의 발현과 예후와의 관계

양석우,조상호,양우익,정우희,안철민,이두연 대한병리학회 1999 Journal of Pathology and Translational Medicine Vol.33 No.12

Flavobacterium meningosepticum의 Nucleoside Oxidase와 Peroxidase 생산특성

최양문,조홍연,양한철 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.6

Flavobacterium meningosepticum로부터 nucleoside oxidase와 peroxidase의 최대생산 조건을 검토한 결과 탄소원으로는 surcose, 질소원으로는 Polypepton이 좋았으며, Fe^2+, Fe^3+이나 또는 이들 이온과 함께 Cu^2+을 배지에 첨가시 두 효소의 생산량은 증가하였다. 또한 2'-deoxyguanosine, 2'-deoxyadenosine, N^6-methyladenosine 및 1-methyladenosine의 첨가는 두 효소의 생산을 증가시켰으며 특히 N^6-methyladenosine과 1-methyladenosine의 첨가는 생산된 균체량은 적었으나 두 효소생산량은 증가시켰다. 배지조건 및 배양시간에 따른 효소생산의 양상을 검토한 결과 두 효소는 거의 유사한 경향을 보였고 효소생산에 미치는 환경인자들 중 산소의 충분한 공급이 필수적이었다. 이상의 결과들로부터 세포내에서 nucleoside가 nucleoside oxidase에 의해 산화될 때 생성되는 H_2O_2를 peroxidase가 분해할 가능성이 시사되었다. Optimal cultural conditions were investigated for the maximal productivity of nucleoside oxidase and peroxidase from Flavobacterium meningosepticum. Sucrose and Polypepton were the best as a carbon source and a nitrogen source. Fe^2+, Fe^3+ and Cu^2+ increased the activities of the two enzymes and were essential in medium containing peptone as a nitrogen source. Nucleoside derivatives such as 2'-deoxyguanosine, 2'-deoxyadenosine, N^6-methyladenosine and 1-methyladenosine were effective for the production of the two enzymes. Especially, the addition of N^6-methyladenosine and 1-methyladenosine decreased cell growth, but increased the two enzyme activities. High level of oxygen also was essential factor for formation and/or induction of these enzymes. From the summary of this study about optimal medium and environmental conditions, nucleoside oxidase was biosynthesized in proportion to peroxidase. These results suggested that the role of peroxidase should be degradation of H_2O_2 generated by nucleoside oxidase in the cell of Flavobacterium meningosepticum.

Flavobacterium meningosepticum이 생산하는 Nucleoside Oxidase의 효소학적 특성

최양문,조홍연,양한철 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

Flavobacterium meningosepticum의 cell free extract로부터 정제된 nucleoside oxidase의 분자량은 480,000이었으며, subunits 구조는 SDS-PAGE에 의해 81,000, 68,000, 32,000과 16,000의 분자량을 갖는 8개 이상의 polypeptide로 구성되어 있었다. 본 효소의 등전점은 5.1이었으며 visible absorption spectra의 결과 전형적인 hemoprotein의 양상을 나타내었으며 효소학적 방법에 의해 정량한 결과 nucleoside oxidase 1 mole당 3 mole의 FAD을 함유하고 있는 flavohemoprotein으로 확인되었다. 본 효소는 1 mM SnCl_2 와 PbCl_2에 의해 각각 94%와 90%의 효소활성이 저해되었으며, hemoenzyme 저해제(NaCN, NaN_3)와 flavoenzyme 저해제(acriflavine, quinacrine)에 의해서도 효소활성이 저해되었다. 효소반응의 최적온도는 50℃, 최적 pH는 7.0이었으며, 60℃ 이하의 온도와 pH 5.5~9.0 범위에서 효소단백질은 안정함을 나타내었다. The molecular weight of the purified nucleoside oxidase estimated by gel filtration column chromatography was 480,000 and the enzyme protein was composed of four nonidentical subnits (81,000, 69,000, 32,000 and 16,000). On the basis of the visible absorption spectra and the enzymatic determination of the purified enzyme, the enzyme was supposed as a hemoprotein and also a flavoprotein containing 3 moles of FAD per 1 mole of enzyme. The isoelectric point of the enzyme was pH 5.1. Addition of metal salts such as 1 mM SnCl_2 and PbCl_2 into an enzyme reaction solution inhibited the enzyme activity by 94 and 90%, respectively. The enzyme activity was also lost significantly by hemoenzyme inhibitors such as NaCN and NaN_3 and flavoenzyme inhibitor, acriflavine and quinacrine. The maximal nucleoside oxidase activity was observed at pH 7.0 and 55℃. The nucleoside oxidase was relatively stable in the range of pH 5.5~9.0 and below 55℃.

Flavobacterium meningosepticum 기원 Peroxidase의 정제 및 특성

최양문,조홍연,양한철 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

Peroxidase를 Flavobacterium meningosepticum의 cell free extract로부터 약 8% 수율로 80배 정제하였다. Peroxidase의 분자량은 Sephacryl S-300으로 측정한 결과 220,000이였으며, subunits는 SDS-FAGE에 의해 54,000의 동일한크기의 4개로 구성되어진 것으로 추정되었다. 본 효소의 visible absorption spectra는 410, 500 및 630 nm에서 최대흡수를, 환원형에서는 435와 500 nm에서 최대흡수를 나타내어 전형적인 hemoprotein으로 관찰되었으며, 등전점은 4.1이였다. 수소공여체로서 N-ethyl-n-(2-hydroxy-3-sulfopropyl)-m-toluidine (Toos)를 사용할 때 반응최적 온도는 50℃, 반응 최적 pH는 5.5였으며, carbonyl reagent 및 Hg^2+ 이온에 의해 효소활성저해를 받았다. Peroxidase was purified to homogeneity from cell free extract of Flavobacterium meningosepticum. The molecular weight of the enzyme estimated by gel filtration column chromatography was 220,000. A identical subunit (54,000) was detected on SDS-PAGE of the enzyme. From these results, the enzyme was supposed to have four identical subunits. On the basis of the visible absorption spectra of the purified enzyme, the enzyme was a typical hemoprotein. The isoelectric point of the enzyme was 4.1. On using N-ethyl-n-(2-hydroxy-3-sulfopropyl)-m-toluidine (Toos) as a hydrogen donor, the enzyme showed optimum activity at the pH 5.5 and 50℃. The enzyme activity was inhibited by carbonyl reagent and Hg^2+.

정신분열병 환자에서 정신병적 증상과 위스콘신카드 분류검사와의 관계

엄양기,김철권,조진석,변원탄 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.5

The purpose of this study was to investigate 1) the difference of performance on the Wisconsin Card Sorting Test(WCST) between schizophrenecs and normal controls, 2) and the change of performance while clinically improved. WCSTs were administered in 28 schizophrenics and 32 normal controls. The results were as follows. 1) Schizophrenic patients performed poorly on the WCST compared to normal controls. 2) There was no significant change of performance in WCST as clinical symptoms improved 3) There was no significant correlation between the performance of the Wisconsin Card Sorting Test and age, sex, education level and volcabulary test in both groups. These findings suggest that the WCST mat be an indicator of vulnerability to schizophrenia.