Classification of soybeans from different habitats based on metabolomic–transcriptomic integration

Wang Jinghui,Zheng Qiyou,Wang Chenxu,Zhou Ao 한국응용생명화학회 2024 Applied Biological Chemistry (Appl Biol Chem) Vol.67 No.-

Soybeans are a significant agricultural product in China, with certain geographical locations often yielding higher quality, and thus more expensive, soybean crops. In this study, metabolomics and transcriptomics analyses were con ducted on soybean samples from nine regions in Heilongjiang and Liaoning Provinces using untargeted liquid chro matography–mass spectrometry (LC–MS) and Illumina sequencing technologies. The primary objective was to devise an effective and unbiased method for determining the geographical origin of each soybean variety to mitigate potential fraudulent practices. Through multidimensional and unidimensional analyses, successful identification of dif ferentially expressed metabolites (DEMs) and differentially expressed genes (DEGs) was achieved, yielding statisti cally significant outcomes. Integration of the metabolomics and transcriptomics datasets facilitated the construction of a correlation network model capable of distinguishing soybeans originating from different geographical locations, leading to the identification of significant biomarkers exemplifying noteworthy distinctions. To validate the feasibil ity of this method in practical applications, partial least squares discriminant analysis was employed to differentiate soybean samples from the nine regions. The results convincingly showcased the applicability and reliability of this approach in accurately pinpointing the geographical origin of soybeans. Distinguishing itself from prior research in soybean traceability, this study incorporates an integrated analysis of metabolomics and transcriptomics data, thereby unveiling biomarkers that offer a more precise differentiation of soybean traits across distinct regions, thereby bridging a critical research gap within the soybean traceability domain. This innovative dual-data integration analysis methodology is poised to enhance the accuracy of soybean traceability tools and lay a new foundation for future agricultural product identification research.

Major ginsenosides from Panax ginseng promote aerobic cellular respiration and SIRT1-mediated mitochondrial biosynthesis in cardiomyocytes and neurons

Huang, Qingxia,Lou, Tingting,Lu, Jing,Wang, Manying,Chen, Xuenan,Xue, Linyuan,Tang, Xiaolei,Qi, Wenxiu,Zhang, Zepeng,Su, Hang,Jin, Wenqi,Jing, Chenxu,Zhao, Daqing,Sun, Liwei,Li, Xiangyan The Korean Society of Ginseng 2022 Journal of Ginseng Research Vol.46 No.6

Background: Aerobic cellular respiration provides chemical energy, adenosine triphosphate (ATP), to maintain multiple cellular functions. Sirtuin 1 (SIRT1) can deacetylate peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) to promote mitochondrial biosynthesis. Targeting energy metabolism is a potential strategy for the prevention and treatment of various diseases, such as cardiac and neurological disorders. Ginsenosides, one of the major bioactive constituents of Panax ginseng, have been extensively used due to their diverse beneficial effects on healthy subjects and patients with different diseases. However, the underlying molecular mechanisms of total ginsenosides (GS) on energy metabolism remain unclear. Methods: In this study, oxygen consumption rate, ATP production, mitochondrial biosynthesis, glucose metabolism, and SIRT1-PGC-1α pathways in untreated and GS-treated different cells, fly, and mouse models were investigated. Results: GS pretreatment enhanced mitochondrial respiration capacity and ATP production in aerobic respiration-dominated cardiomyocytes and neurons, and promoted tricarboxylic acid metabolism in cardiomyocytes. Moreover, GS clearly enhanced NAD⁺-dependent SIRT1 activation to increase mitochondrial biosynthesis in cardiomyocytes and neurons, which was completely abrogated by nicotinamide. Importantly, ginsenoside monomers, such as Rg1, Re, Rf, Rb1, Rc, Rh1, Rb2, and Rb3, were found to activate SIRT1 and promote energy metabolism. Conclusion: This study may provide new insights into the extensive application of ginseng for cardiac and neurological protection in healthy subjects and patients.

An Efficient PSI-CA Protocol Under the Malicious Model

Jingjie Liu,Suzhen Cao,Caifen Wang,Chenxu Liu 한국인터넷정보학회 2024 KSII Transactions on Internet and Information Syst Vol.18 No.3

Private set intersection cardinality (PSI-CA) is a typical problem in the field of secure multi-party computation, which enables two parties calculate the cardinality of intersection securely without revealing any information about their sets. And it is suitable for private data protection scenarios where only the cardinality of the set intersection needs to be calculated. However, most of the currently available PSI-CA protocols only meet the security under the semi-honest model and can’t resist the malicious behaviors of participants. To solve the problems above, by the application of the variant of Elgamal cryptography and Bloom filter, we propose an efficient PSI-CA protocol with high security. We also present two new operations on Bloom filter called IBF and BIBF, which could further enhance the safety of private data. Using zero-knowledge proof to ensure the safety under malicious adversary model. Moreover, in order to minimize the error in the results caused by the false positive problem, we use Garbled Bloom Filter and key-value pair packing creatively and present an improved PSI-CA protocol. Through experimental comparison with several existing representative protocols, our protocol runs with linear time complexity and more excellent characters, which is more suitable for practical application scenarios.

Researching on X-Band Electromagnetic Interference Shielding Efficiency of MWCNTs Buckypapers Inserted with Mn Nanopowder

Shaowei Lu,Yaoyao Bai,Jijie Wang,Chenxu Zhao,Wei Li,Xiaoqiang Wang,Zhenwei Huang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.6

Ultrathin multi-walled carbon nanotubes (MWCNTs) buckypapers inserted with Mn nanopowder were fabricated via vacuum filtration method, at a lower manufacturing cost and easier operation. The buckypapers were characterized structurally using scanning electron microscopy, energy-dispersive spectrometer and X-ray diffraction. Electromagnetic interference (EMI) shielding effectiveness (SE) of MWCNTs buckypapers inserted with Mn nanopowder (0–30 wt.%) were tested in X-band (8.2–12.4 GHz). When the blended Mn nanopowder content is 20 wt.%, the buckypaper exhibits much higher EMI SE of average value up to 42 dB with the thickness of only 0.11 mm. Further, this buckypaper is flexible and lightweight, and can be integrated with resin matrix composites. Thus, the results and techniques promise a simple and effective approach to achieve lightweight and ultrathin composite films for a wide application prospect in the field of EMI shielding and microwave absorption.

Progressive Collapse Analysis of Large-span Reticulated Domes

Qinghua Han,Mingjie Liu,Yan Lu,Chenxu Wang 한국강구조학회 2015 International Journal of Steel Structures Vol.15 No.2

The paper presents the progressive collapse analysis of large-span reticulated domes based on Alternate path (AP) method. Nonlinear buckling analysis is conducted on a large span roof with two different structural layouts. The results show that although large-span single-layer reticulated dome has a certain redundancy. When 10 key elements or 3 key nodes were removed, the single-layer reticulated dome under the service load would cause progressive collapse. Therefore the protection of key nodes of this structure should be strengthened. However, the redundancy of large-span double-layer reticulated dome is 7 to 13 times that of single-layer reticulated dome. When 10 key elements or 3 key nodes were removed, the double-layer reticulated dome under the service load would not cause progressive collapse. Hence, the resistance to progressive collapse of large-span double-layer reticulated dome is better than that of large-span single-layer reticulated dome.

Navigation safety domain and collision risk index for decision support of collision avoidance of USVs

Zhou Jian,Ding Feng,Yang Jiaxuan,Pei Zhengqiang,Wang Chenxu,Zhang Anmin 대한조선학회 2021 International Journal of Naval Architecture and Oc Vol.13 No.1

This paper proposes a decision support model for USVs to improve the accuracy of collision avoidance decision-making. It is formed by Navigation Safety Domain (NSD) and domain-based Collision Risk Index (CRI), capable of determining the collision stage and risk between multiple ships. The NSD is composed of a warning domain and a forbidden domain, which is constructed under the constraints of COLREGs (International Regulations for Preventing Collisions at Sea). The proposed domain based CRI takes the radius of NSD in various encounter situations as threshold parameters. It is found that the value of collision risk in any directions can be calculated, including actual value and risk threshold. A catamaran USV and 6 given vessels are taken as study objects to validate the proposed model. It is found that the judgment of collision stage is accurate and the azimuth range of risk exists can be detected, hence the ships can take direct and effective collision avoidance measures. According to the relation between the actual value of CRI and risk threshold, the decision support rules are summarized, and the specific terms of COLREGs to be followed in each encounter situation are given.

Efficiently targeted therapy of glioblastoma xenograft via multifunctional biomimetic nanodrugs

Zhipeng Yao,Xiaochun Jiang,Hong Yao,Yafeng Wu,Fan Zhang,Cheng Wang,Chenxue Qi,Chenhui Zhao,Zeyu Wu,Min Qi,Jia Zhang,Xiaoxiang Cao,Zhichun Wang,Fei Wu,Chengyun Yao,Songqin Liu,Shizhang Ling,Hongping Xi 한국생체재료학회 2022 생체재료학회지 Vol.26 No.4

Background: Glioblastoma multiforme (GBM) is a fatal malignant primary brain tumor in adults. The therapeutic efficacy of chemotherapeutic drugs is limited due to the blood-brain barrier (BBB), poor drug targeting, and short biological half-lives. Multifunctional biomimetic nanodrugs have great potential to overcome these limitations of chemotherapeutic drugs. Methods: We synthesized and characterized a biomimetic nanodrug CMS/PEG-DOX-M. The CMS/PEG-DOX-M effectively and rapidly released DOX in U87 MG cells. Cell proliferation and apoptosis assays were examined by the MTT and TUNEL assays. The penetration of nanodrugs through the BBB and anti-tumor efficacy were investigated in the orthotopic glioblastoma xenograft models. Results: We showed that CMS/PEG-DOX-M inhibited cell proliferation of U87 MG cells and effectively induced cell apoptosis of U87 MG cells. Intracranial antitumor experiments showed that free DOX hardly penetrated the BBB, but CMS/PEG-DOX-M effectively reached the orthotopic ntracranial tumor through the BBB and significantly inhibited tumor growth. Immunofluorescence staining of orthotopic tumor tissue sections confirmed that nanodrugs promoted apoptosis of tumor cells. This study developed a multimodal nanodrug treatment system with the enhanced abilities of tumor-targeting, BBB penetration, and cancer-specific accumulation of chemotherapeutic drugs by combining chemotherapy and photothermal therapy. It can be used as a flexible and effective GBM treatment system and it may also be used for the treatment of other central nervous systems (CNS) tumors and extracranial tumors.

One-step solvothermal synthesis of poly(arylene ether nitrile) decorated magnetic composite for methylene blue adsorption from aqueous solution

Zhou Xuefei,Miao Wei,Cheng Wenxi,Lin Haowei,Lin Haowei,Zheng Hongjuan,Cheng Qiaohuan,Wang Renjie,Yao Chenxue,Liu Xiaobo 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.6

A series of poly(arylene ether nitrile) containing versatile carboxyl and sulfonic groups (CSPEN) were magnetically functionalized by ferroferric oxide (Fe3O4), and the obtained magnetic adsorbents (Fe3O4/CSPEN) were used to remove methylene blue (MB) dye from aqueous solution. The systematical characterizations that including scanning electron microscope (SEM), X-ray powder diffractometer (XRD), X-ray photoelectron spectroscopy (XPS), vibrating sample magnetometer (VSM), Fourier transform infrared (FTIR) certified that the Fe3O4/CSPEN possessed versatile functional groups and magnetic separation properties. The batch adsorption studies revealed that the Fe3O4/CSPEN not only displayed high selective adsorption ability for cationic MB in the presence of anionic MO, but also exhibited a removal efficiency as high as 98.2%. Besides, the adsorption kinetics and isotherm matched well with pseudo-second-order model and Langmuir model, respectively, and the maximum adsorption capacity of Fe3O4/CSPEN for MB was 92.029 mg/g. The FTIR and Brunauer-Emmett-Teller (BET) analyses confirmed that the outstanding adsorption capacity of Fe3O4/CSPEN was attributed to their own microporous structure and the electrostatic interaction with MB. Therefore, the modified magnetic adsorbent can be used to selectively remove cationic dye from aqueous solution.

N-Heterocyclic Carbene Adducts of Cyclopalladated Ferrocenylpyridine Complex: Synthesis, Structural Characterization and Application in Heck Reaction

Chen Xu,Lu-Meng Duan,Zhen Li,Xin-Hua Lou,Zhi-Qiang Wang,Yao-Ting Fan 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

no abstract

N-Heterocyclic Carbene-Tricyclohexylphosphine Palladium(II) Complex: Synthesis, Crystal Structure and Application in Suzuki Reaction of Aryl Chlorides

Chen Xu,Xin-Qi Hao,Zhen Li,Lu-Meng Duan,Zhi-Qiang Wang,Bao-Ming Ji,Mao-Ping Song 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.10