THE RELIABLE DESIGN OF THE CIM NETWORK FOR A LARGE-SCALE MACHINE TOOL FACTORY

Yan, Bao Ping,Zhou, Jin Ming,Bao Shcng Hu 대한전자공학회 1992 HICEC:Harbin International Conference on Electroni Vol.1 No.1

This paper discusses the reliability problems during designing the JFMT (Jinan First Machine Tool works) CIM network. At first the sources which affect the reliability of JFMT CIM network are analysed. And then some solutions for enhancing the reliability of JFMT CIM network are given. These methods are also suitable to other CIM networks design and of certain practicality.

Glucosamine induces cell death via proteasome inhibition in human ALVA41 prostate cancer cell

Bao-Qin Liu,Hua-Qin Wang,Xin Meng,Chao Li,Yan-Yan Gao,Ning Li,Xiao-Fang Niu,Yifu Guan 생화학분자생물학회 2011 Experimental and molecular medicine Vol.43 No.9

Glucosamine, a naturally occurring amino monosaccharide,has been reported to play a role in the regulation of apoptosis more than half century. However the effect of glucosamine on tumor cells and the involved molecular mechanisms have not been thoroughly investigated. Glucosamine enters the hexosamine biosynthetic pathway (HBP) downstream of the rate-limiting step catalyzed by the GFAT (glutamine:fluctose-6-phosphate amidotransferase), providing UDPGlcNAc substrates for O-linked β-N-acetylglucosamine (O-GlcNAc) protein modification. Considering that O-GlcNAc modification of proteasome subunits inhibits its activity, we examined whether glucosamine induces growth inhibition via affecting proteasomal activity. In the present study, we found glucosamine inhibited proteasomal activity and the proliferation of ALVA41 prostate cancer cells. The inhibition of proteasomal activity results in the accumulation of ubiquitinated proteins, followed by induction of apoptosis. In addition, we demonstrated that glucosamine downregulated proteasome activator PA28γ and overexpression of PA28γ rescued the proteasomal activity and growth inhibition mediated by glucosamine. We further demonstrated that inhibition of O-GlcNAc abrogated PA28γ suppression induced by glucosamine. These findings suggest that glucosamine may inhibit growth of ALVA41 cancer cells through downregulation of PA28γ and inhibition of proteasomal activity via O-GlcNAc modification.

Comparison of the Nutritional Composition of Bullfrog Meat from Different Parts of the Animal

Yanli Zhu,Min Bao,Chong Chen,Xiaoli Yang,Wenliang Yan,Fazheng Ren,Pengjie Wang,Pengcheng Wen 한국축산식품학회 2021 한국축산식품학회지 Vol.41 No.6

The objective of this study was to evaluate the nutritional content of bullfrog meat from different parts of the animal, including fore-chest, thigh and calf. Bullfrog meat was found to be a rich source of proteins, essential amino acids and minerals, but with a low fat content, compared with other aquatic meat products. There was no significant difference (p>0.05) between thigh and calf in mineral content (K, P, Na, Mg, Ca, Zn, Fe, Cu, and Mn), but the contents of K, P, and Mg were higher in thigh and calf than in the fore-chest (p<0.05). The salt-soluble, water-soluble and insoluble protein bands in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis, from fore-chest, thigh and calf were similar, with the most abundant bands being 35 kDa (salt-soluble protein), 35-48 kDa (water-soluble protein) and 48 kDa (insoluble protein). The results showed that the insoluble protein content in the fore-chest meat was higher than that in the thigh meat and calf meat, but the salt-soluble protein fraction was the most abundant in thigh meat. These results showed that the nutrients in different parts of bullfrog meat were different.

Sonochemical synthesis of flower-like ZnO assembled by hollow cones toward water vapor permeability and water resistance enhancement of waterborne film

Yan Bao,Lu Gao,Caiping Feng,Jianzhong Ma,Wenbo Zhang,Chao Liu,Demetra Simion 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.82 No.-

In this study,flower-like ZnO assembled by hollow cones (F-ZnO-HCs) were synthesized viasonochemical route and the related morphology and structure of product were characterized. Then,F-ZnO-HCs nanostructures were employed to polyacrylate and the effect of F-ZnO-HCs on the propertiesof waterbornefilms was investigated. Meanwhile, the morphological evolution of F-ZnO-HCs wasproposed and the superiority of F-ZnO-HCs on synchronously improving the water resistance and watervapor permeability of polyacrylatefilm was discussed. The results showed that F-ZnO-HCs possessed anaverage diameter of800 nm and a large specific surface area of 16.01 m2 g 1. Adjusting the watercontent and citric acid dosage, the morphology of ZnO transformed from F-ZnO-HCs to ZnOnanoparticles, F-ZnO-NSs, F-ZnO-SCs and F-ZnO-NRs. F-ZnO-HCs nanostructures asfillers not onlysignificantly enhance the stability of polyacrylate latex but also simultaneously improve the water vaporpermeability and water resistance of polyacrylatefilm. Moreover, compared with other morphologicalZnO, F-ZnO-HCs was the best for simultaneously improving the water resistance and water vaporpermeability of polyacrylatefilm. F-ZnO-HCs is an ideal candidate for resolving the contradiction of thewater resistance and water vapor permeability of waterbornefilms.

THE RELIABLE DESIGN OF THE CIM NETWORK FOR A LARGE-SCALE MACHINE TOOL FACTORY

Bao Ping Yan,Jin Ming Zhou,Bao Shcng Hu 대한전자공학회 1992 대한전자공학회 Conference Vol.1992 No.2

Genome-wide identification and analysis of long noncoding RNAs in longissimus muscle tissue from Kazakh cattle and Xinjiang brown cattle

Yan, Xiang-Min,Zhang, Zhe,Liu, Jian-Bo,Li, Na,Yang, Guang-Wei,Luo, Dan,Zhang, Yang,Yuan, Bao,Jiang, Hao,Zhang, Jia-Bao Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.11

Objective: In recent years, long noncoding RNAs (lncRNAs) have been identified in many species, and some of them have been shown to play important roles in muscle development and myogenesis. However, the differences in lncRNAs between Kazakh cattle and Xinjiang brown cattle remain undefined; therefore, we aimed to confirm whether lncRNAs are differentially expressed in the longissimus dorsi between these two types of cattle and whether differentially expressed lncRNAs regulate muscle differentiation. Methods: We used RNA-seq technology to identify lncRNAs in longissimus muscles from these cattle. The expression of lncRNAs were analyzed using StringTie (1.3.1) in terms of the fragments per kilobase of transcript per million mapped reads values of the encoding genes. The differential expression of the transcripts in the two samples were analyzed using the DESeq R software package. The resulting false discovery rate was controlled by the Benjamini and Hochberg's approach. KOBAS software was utilized to measure the expression of different genes in Kyoto encyclopedia of genes and genomes pathways. We randomly selected eight lncRNA genes and validated them by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Results: We found that 182 lncRNA transcripts, including 102 upregulated and 80 downregulated transcripts, were differentially expressed between Kazakh cattle and Xinjiang brown cattle. The results of RT-qPCR were consistent with the sequencing results. Enrichment analysis and functional annotation of the target genes revealed that the differentially expressed lncRNAs were associated with the mitogen-activated protein kinase, Ras, and phosphatidylinositol 3-kinase (PI3k)/Akt signaling pathways. We also constructed a lncRNA/mRNA coexpression network for the PI3k/Akt signaling pathway. Conclusion: Our study provides insights into cattle muscle-associated lncRNAs and will contribute to a more thorough understanding of the molecular mechanism underlying muscle growth and development in cattle.

Acupuncture for Treatment of Erectile Dysfunction: A Systematic Review and Meta-Analysis

Bao-yong Lai,Hui-juan Cao,Guo-yan Yang,Li-yan Jia,Suzanne Grant,Yu-tong Fei,Emma Wong,Xin-lin Li,Xiao-ying Yang,Jian-ping Liu 대한남성과학회 2019 The World Journal of Men's Health Vol.37 No.3

ChinaPurpose: To assess the effectiveness and safety of acupuncture for erectile dysfunction (ED).Materials and Methods: We searched six major English and Chinese databases included randomized controlled trials (RCTs) testing acupuncture alone or in combination for ED. Dichotomous data were presented as risk ratio (RR) and continuous data were presented as mean difference (MD) both with 95% confidence interval (CI). The Revman (v.5.3) was used for data analyses. Quality of evidence across studies was assessed by the online GRADEpro tool.Results: We identified 22 RCTs, fourteen of them involving psychogenic ED. Most of the included RCTs had high or unclear risk of bias. There was no difference between electro-acupuncture and sham acupuncture with electrical stimulation on the rate of satisfaction and self-assessment (RR, 1.50; 95% CI, 0.71–3.16; 1 trial). Acupuncture combined with tadalafil appeared to have better effect on increasing cure rate (RR, 1.31; 95% CI, 1.00–1.71; 2 trials), and International Index of Erectile Function-5 scores (MD, 5.38; 95% CI, 4.46–6.29; 2 trials). When acupuncture plus herbal medicine compared with herbal medicine alone, the combination therapy showed significant better improvement in erectile function (RR, 1.68; 95% CI, 1.31–2.15; 7 trials). Only two trials reported facial red and dizziness cases, and needle sticking and pruritus cases in acupuncture group. Conclusions: Low quality evidence shows beneficial effect of acupuncture as adjunctive treatment for people mainly with psychogenic ED. Safety of acupuncture was insufficiently reported. The findings should be confirmed in large, rigorously designed and well-reported trials.

Using weighted gene co-expression network analysis (WGCNA) to identify the hub genes related to hypoxic adaptation in yak (Bos grunniens)

Bao Qi,Zhang Xiaolan,Bao Pengjia,Liang Chunnian,Guo Xian,Chu Min,Yan Ping 한국유전학회 2021 Genes & Genomics Vol.43 No.10

Background As a mammal living at the highest altitude in the world, the yak has strong adaptability to the harsh natural environment (such as low temperature, scarce food, especially low oxygen) of Qinghai-Tibet Plateau (QTP) after a long process of natural selection. Objective Here, we used Weighted Correlation Network Analysis (WGCNA), a systematic biology method, to identify hypoxic adaptation-related modules and hub genes. The research of the adaptability of yak against hypoxia is of great signifcance to identify the genetic characteristics and yak breeding. Methods Based on the transcriptome sequencing data (PRJNA362606), the R package DESeq2 and WGCNA were conducted to analyze diferentially expressed genes (DEGs) and construct the gene co-expression network. The module hub genes were identifed and characterized by the correlation of gene and trait, module membership (kME). In addition, GO and KEGG enrichment analyses were used to explore the functions of hub genes. Results Our results revealed that 1098, 1429, and 1645 DEGs were identifed in muscle, spleen, and lung, respectively. Besides, a total of 13 gene co-expression modules were detected, of which two hypoxic adaptation-related modules (saddlebrown and turquoise) were found. We identifed 39 and 150 hub genes in these two modules. Functional enrichment analyses showed that 12 GO terms and 18 KEGG pathways were enriched in the saddlebrown module while 85 GO terms and 22 KEGG pathways were enriched in the turquoise module. The signifcant pathways related to hypoxia adaptation include FoxO signaling pathway, Thermogenesis pathway, and Retrograde endocannabinoid signaling pathway, etc. Conclusions In this study, we obtained two hypoxia-related specifc modules and identifed hub genes based on the connectivity by constructing a weighted gene co-expression network. Function enrichment analysis of two modules revealed mitochondrion is the most important organelle for hypoxia adaptation. Moreover, the insulin-related pathways and thermogenic-related pathways played a major role. The results of this study provide theoretical guidance for further understanding the molecular mechanism of yak adaptation to hypoxia.

Cationic silicon-based gemini surfactants: Effect of hydrophobic chains on surface activity, physic-chemical properties and aggregation behaviors

Yan Bao,Jiajia Guo,Jianzhong Ma,Pan Liu,Qiaoling Kang,Jing Zhang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.53 No.-

A series of cationic silicon-based gemini surfactants (Cm-PSi-Cm) having different hydrophobic chains length (m = 8,10,12,14,16,18) were synthesized. Subsequently, surface parameters including equilibrium surface tension (gCMC), critical micelle concentration (CMC), surface pressure at CMC (PCMC) and maximum surface excess (Gmax) were calculated according to the surface tension measurements. The importance of hydrophobic chains length on the surface activity of Cm-PSi-Cm was revealed in detail. With the increasing hydrophobic chains length, the equilibrium surface tension (gCMC) values follow the order of C8-PSi-C8 > C10-PSi-C10 > C12-PSi-C12< C14-PSi-C14< C16-PSi-C16< C18-PSi-C18. The thermodynamic parameters of micellization process, namely, standard Gibbs free energy (DG0 m), enthalpy (DH0 m) and entropy (DS0 m), were derived from conductivity measurements at different temperatures. The aggregation of the cationic silicon-based gemini surfactants (C12-PSi-C12) in aqueous solution at different concentrations was studied by DLS and TEM measurements. The formation of micelles and vesicles in C12-PSi-C12 solutions was observed at a concentration well above the CMC. Moreover, the morphology of vesicles changed from sphere to rod-like, dumbble-like and finally string dumbbell-like with the increasing of the surfactant concentration.

Molecular organization of the B mating type locus of a Lentinula edodes monokaryon strain SUP2

Bao Da-Peng Chen Ming-Jie,Song Wen-Hua,Song Chun-yan,Zhang Mei-Yan,Chen Xiang,Lin Nan 한국버섯학회 2010 한국버섯학회지 Vol.8 No.4

Lentinula edodes is an important cultivated mushroom in China. The development of Lentinula edodes production promotes more studies on it. In our previous work, degenerate PCR and chromosome walking technologies were used to obtain one pheromone receptor gene and one pheromone precursor gene from Lentinula edodes. In this study, four pairs of specific primers were designed according to the whole genome sequencing of the protoplast monokaryon of Lentinula edodes strain 135, to amplify STE3-like pheromone receptor gene and its flanking conserved genes in the protoplast monokaryon strain SUP2 derived from Lentinula edodes strain Suxiang and 33655bp DNA sequence was obtained. By BlastX search, seven putative genes were identified, and three of them are pheromone receptor encoded genes. Furthermore, near to two pheromone receptor genes, four genes encoding proteins with conserved motifs of pheromone precursors were found. This study firstly reveals the molecular organization of the B mating type locus of Lentinula edodes.