Using weighted gene co-expression network analysis (WGCNA) to identify the hub genes related to hypoxic adaptation in yak (Bos grunniens)

Bao Qi,Zhang Xiaolan,Bao Pengjia,Liang Chunnian,Guo Xian,Chu Min,Yan Ping 한국유전학회 2021 Genes & Genomics Vol.43 No.10

Background As a mammal living at the highest altitude in the world, the yak has strong adaptability to the harsh natural environment (such as low temperature, scarce food, especially low oxygen) of Qinghai-Tibet Plateau (QTP) after a long process of natural selection. Objective Here, we used Weighted Correlation Network Analysis (WGCNA), a systematic biology method, to identify hypoxic adaptation-related modules and hub genes. The research of the adaptability of yak against hypoxia is of great signifcance to identify the genetic characteristics and yak breeding. Methods Based on the transcriptome sequencing data (PRJNA362606), the R package DESeq2 and WGCNA were conducted to analyze diferentially expressed genes (DEGs) and construct the gene co-expression network. The module hub genes were identifed and characterized by the correlation of gene and trait, module membership (kME). In addition, GO and KEGG enrichment analyses were used to explore the functions of hub genes. Results Our results revealed that 1098, 1429, and 1645 DEGs were identifed in muscle, spleen, and lung, respectively. Besides, a total of 13 gene co-expression modules were detected, of which two hypoxic adaptation-related modules (saddlebrown and turquoise) were found. We identifed 39 and 150 hub genes in these two modules. Functional enrichment analyses showed that 12 GO terms and 18 KEGG pathways were enriched in the saddlebrown module while 85 GO terms and 22 KEGG pathways were enriched in the turquoise module. The signifcant pathways related to hypoxia adaptation include FoxO signaling pathway, Thermogenesis pathway, and Retrograde endocannabinoid signaling pathway, etc. Conclusions In this study, we obtained two hypoxia-related specifc modules and identifed hub genes based on the connectivity by constructing a weighted gene co-expression network. Function enrichment analysis of two modules revealed mitochondrion is the most important organelle for hypoxia adaptation. Moreover, the insulin-related pathways and thermogenic-related pathways played a major role. The results of this study provide theoretical guidance for further understanding the molecular mechanism of yak adaptation to hypoxia.

Bisindole Alkaloids of the Topsentin and Hamacanthin Classes from a Marine Sponge Spongosorites sp.

Bao Quan Bao,Qi Shi Sun,Xin Sheng Yao,Jong Ki Hong,Chong O Lee,Hee Young Cho,Jee H. Jung 경희대학교 동서약학연구소 2008 경희약대 논문집 Vol.35 No.1

Investigation on the Current Situation of China's Internet Arbitration and Suggestions for its Perfection

Bao, Bing-Feng,Yang, Ya-Qi 한중법학회 2019 中國法硏究 Vol.39 No.-

국가의 정보화 발전전략이 부단히 추진되고 있는 상황에서 국가주도 의 자원형식이 점차적으로 공공자원, 사회자원, 시장자원 등의 다원화 된 주체의 형식으로 변화하고 있으며, 분쟁해결에 있어서도 소송의 이 른바 “외나무다리(独木桥)”라는 현상을 맞으면서 화해, 조정, 중재 등과 상호 관련하여 해결할 수 있는 시스템으로 발전하고 있다. 분쟁해결에 대하여 인민 대중의 만족을 위하여 인터넷 중재시스템의 혁신과 발전의 시기를 마주하고 있다. 인터넷 중재는 인터넷 비즈니스 분쟁에 착안하 여 인터넷 플랫폼인 전자기술과 빅데이터, 클라우드 등의 종합적 사용방안을 활용하여, 당사자에게 분쟁해결서비스를 제고할 수 있을 것이라 보고 있다. 이 에 분쟁안건의 “양(量)”을 분류하고, 분쟁해결의 실현에 있어 “질(质)”적 개선 을 촉진할 수 있을 것이다. 그리고 인터넷 중재는 중국에서 보편적이지 않아 중재 의식고 신뢰가 부족하므로 다양한 면에서 장애가 존재한다. 이러한 장애 를 제거하는 관건은 법률의 강력한 보장과 실무에서의 효율성 제고에 있다. 이 에 기초하여, 첫 번째는 입법적 통일을 통하여 인터넷 중재의 전체 구조를 확 립함으로써 건전한 법률체계를 포함하여 인터넷 신뢰표준제도 및 인터넷 중재 판결서의 송달과 집행을 보장하여야 한다. 둘째, 필요한 기술을 통하여 플랫폼 시스템과 안건 처리에 있어 빅데이터를 탄력적으로 운영한다. 또한, 일반 대중 의 인터넷 중재에 관한 의식을 제고할 수 있도록 한다. 이를 통해 인터넷 중재 가 발휘할 수 있는 효능을 보장할 수 있으며, 인터넷 중재시스템이나 새로운 유형의 분쟁해결시스템에 대한 정보화 발전을 촉진할 것이다. In the present moment when the National Information Development strategy is constantly advancing, the state-dominated public resource model has gradually been transformed into a multi-subject model of public resources, social resources, market resources, etc. In the dispute resolution channel, litigation has also evolved into a mutually applicable mechanism of conciliation, mediation, arbitration and so on. In order to meet the diverse needs of the people for dispute resolution, the innovation and development of online arbitration is timely. Internet arbitration focuses on Internet business disputes and integrates the comprehensive use of electronic technology, big data, and cloud computing on the Internet platform to provide parties with convenient and efficient dispute resolution services. As a result, the “quantity” diversion of dispute cases is promoted, and the “quality” improvement of dispute resolution is realized. However, the promotion of Internet arbitration in China is not universal. It faces many obstacles, such as lack of arbitration consciousness, credit concepts and so on. The key to removing obstacles lies in the strong guarantee of law and the optimization of practical operation. Based on this, on the one hand, it is necessary to unify and perfect the overall framework of Internet arbitration through legislation, including perfecting the supporting legal system, establishing the online trust stamp system, and ensuring the service and enforcement of Internet arbitration awards. On the other hand, we need science and technology to help online platform systems and a flexible use of big data to handle case data, while standardizing the industry personnel and enhancing public awareness of arbitration. Only in this way can we effectively guarantee the effectiveness of the Internet arbitration system and promote the information development of the online arbitration institutions and the new dispute resolution mechanism.

Effect of Vanadium on the Microstructure and Mechanical Properties of 2100 MPa Ultra-High Strength High Plasticity Spring Steel Processed by a Novel Online Rapid-Induction Heat Treatment

Qi‑Lei Dai,Kun Li,Kai‑Ren Meng,Zhou Fang,Wen Chen,Tian‑Bao Yang,Chi Feng,Jin‑Ming Wu,R. D. K. Misra 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.4

Advanced automotive industries generate large demand for the next generation of high strength and high toughness springsteels. Vanadium-containing 55SiCrV spring steels subjected to rapid-induction heating treatment can fulfil such requirements. However, the effect of vanadium microalloying under online rapid-induction heat treatments is rarely reported. Acomparative study of the microstructure and tensile properties of 55SiCr and 55SiCrV spring steel wires subjected to a novelonline rapid induction heat treatment has been demonstrated herein. It is found that the tensile strength of the 55SiCr springwire decreases with the decrease in the wire speed in online rapid-induction heating, and the plasticity increases. Whereas,the tensile strength of the 55SiCrV steel wire increases with the decrease in the wire speed with the retained high plasticity,which is attributed to the strengthening effect of the dislocations. Through the optimized rapid-induction heating/coolingthermal cycles and intermediate-temperature tempering treatment, the tensile strength of the 55SiCrV steel wire approaches2106 MPa with total elongation of 9.7%. Compared with the 55SiCr spring steel, the addition of V in 55SiCrV spring steelchanges the strengthening and toughening mechanisms via the grain refinement and enhancement in the hardenability andtempering resistance. The finely dispersed V-containing secondary phases are rarely found in the matrix, which indicates thatthe precipitation effect stemming from the addition of V is not the dominant strengthening factor in the online rapid-inductionheat process. The proposed novel online rapid-induction heat treatment provides a promising pathway for the mechanicalproperty improvement of the spring steel.

Involvement of MicroRNA-198 Overexpression in the Poor Prognosis of Esophageal Cancer

Qi, Bo,Yao, Wen-Jian,Zhao, Bao-Sheng,Qin, Xiu-Guang,Wang, Yi,Wang, Wen-Ju,Wang, Tian-Yun,Liu, Shang-Guo,Li, Han-Chen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.9

Objective: This study aimed to investigate whether the miR-198 expression level is related to clinicopathological factors and prognosis of esophageal cancer. Methods: MicroRNA was extracted from esophageal cancer patients who underwent surgery for assessment using the Taqman@ MicroRNA assay. The correlation between miR-198 expression and clinicopathological features was analyzed, and the significance of miR-198 as a prognostic factor and its relationship with survival was determined. Results: MicroRNA-198 (miR-198) expression was higher in patients with poor prognosis than those with good prognosis (P<0.05). Kaplan-Meier analysis results showed that the miR-198 expression level had a significant correlation with survival time (P=0.030) and that patients with a higher expression of miR-198 had a shorter survival time. Cox multi-factor model analysis showed that patient prognosis (P=0.014), tumor length (P=0.040) and expression (P=0.012), and survival time had a significant correlation; the corresponding risks were 7.268, 1.246, and 3.524, respectively. Conclusion: miR-198 overexpression is involved in the poor prognosis of esophageal cancer and can be used as a biomarker for selection of cases requiring especial attention.

Effects of Elevated Ozone on Soil Microbial Community Composition and Metabolic Diversity Depending on the Ozone-Tolerance of Wheat Cultivars

Qi Li,Xuelian Bao,Wenju Liang,Caiyan Lu,Jianguo Zhu 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Laboratory evaluation of differential attraction of Culex pipiens pallens to the volatiles of flowers, fruits, and seed pods

Bao-Ting Yu,Shu-Qi Huang,Yan-Mei Ding,Hatem Fouad,Hong-Jie Li,Jian-Chu Mo 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4

The attraction of Culex pipiens pallens Coquillett to various potential sugar sources in their habitats remains unknown. In the present study, we evaluated the attraction of Cx. pipiens pallens to the volatiles of different kinds of flowers, fruits, and seed pods by multiple choice tests under laboratory conditions. Nine of the 21 tested fresh tissues of flowers were attractive to females and six of them were attractive to males. The volatiles of six flowers (Ligustrum quihoui, Abelia chinensis, Ophiopogon japonicus, Phaseolus vulgaris, Osmanthus fragrans, and Nerium indicum) were attractive to both female and male Cx. pipiens pallens, and the flowers of Torenia fournieri and Lavandula angustifolia were potentially repellent to female and male mosquitoes, respectively. Besides, the volatiles of three fruits and seed pods (Broussonetia papyrifera, Amygdalus persica, and Cucumis melo) were attractive to both female and male Cx. pipiens pallens while the seed pods of Ficus carica and Pyrus bretschneideri were only attractive to females and males, respectively. This study provides valuable information on developing novel control methods against Cx. pipiens pallens in China.

HPLC Analysis of Citrinin in Red Yeast Rice

Bao-jun Xu,Qi-jun Wang,Jeong-hyun Lee,Xiao-qin Jia,Chang-keun Sung 한국식품과학회 2003 Food Science and Biotechnology Vol.12 No.4

Dynamic File Grouping for Load Balancing in Streaming Media Clustered Server Systems

Qi Jiang,Hong-Sheng Xi,Bao-Chen Yin 제어·로봇·시스템학회 2009 International Journal of Control, Automation, and Vol.7 No.4

A dynamic file grouping strategy is presented to address the load balancing problem in streaming media clustered server systems. This strategy increases the server cluster availability by balancing the workloads among the servers within a cluster. Additionally, it improves the access hit ratio of cached files in delivery servers to alleviate the limitation of I/O bandwidth of storage node. First, the load balancing problem is formulated as a two layers semi-Markov switching state-space control process. This analytic model captures the behaviors of streaming media clustered server systems accurately, and is with constructional flexibility and scalability. Then, a policy iteration based reinforcement learning algorithm is proposed to optimize the file grouping policy online. By utilizing the features of the event-driven policy, the proposed optimization algorithm is adaptive and with less computational cost. Simulation results demonstrate the effectiveness of the proposed approach.

Design of Control System for Bearing Tester

Long-Qi Zu,Bao-Ying Li,Yang Chen 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

A design of control system used in bearing parameter tester is introduced, using GE company PLC (programmable logic controller) as main control unit, PT (programmable Terminal) as data display & control unit, IPC (Industry Processing Computer) as monitor unit, by means of the communication between IPC and PLC, PLC and PT, control system realized the parameter test for the special bearing. The designed programmer on PLC realized bearing parameter test for example pressure, velocity, PV(present value) value and so on. The designed programmer on PT realized that bearing parameter test have all kind functions for example manual running, auto-running, simulation running and so on. It has been testified that the system has higher reliability, tested bearing parameter up to scratch and better property.