서울 시내 일부 대학생의 성지식과 성태도에 관한 연구

김보배,노은경,박서영,박영,오승희,전영란,차현진,윤희상,김수지,이자형 이화여자대학교 간호과학대학 2004 이화간호학회지 Vol.- No.38

This study surveyed and analyzed comparatively college students-knowledge' attitude, was to cultivate the right knowledge and attitude on sex, and serve the foundational data for the suitable sex education. The purpose of the study was to identify and examine: 1) students; sexual knowledge and sexual attitude; 2) the differences between variables of gender, age, and self-perceived sexual knowledge in relation to sexual knowledge and sexual attitude; and 3) the relationship between sexual knowledge and sexual attitude. For this study the questionaires were given to 300 students from five different universities located seoul areas. the questionaires were collected Jenuary 8th to 15th in 2004. Two hundred and nigty one samples were analyzed for the study. The adapted SKAT(saxual knowledge & attitude test; Lief & Reed, 1986) was used to determine sexual knowledge and the students' sexual attitude. Collected data were analyzed statistically by means of percentage, t-test, ANOVA test, and pearson correlation analysis with SPSS 12.0 package program. The main results of this analysis are as follows: Participants consisted of male(48.1%) and female(51.9%) which were mainly between the ages of 21.25(71.8%) with an age range of 19 to 30. Knowledge of sexual disease was scored highest of 92.4%, and konwledge of the knowledge of the male and female's anatomy was the lowest at 60.9%. The respondents' level of sexual attitude was tolerant as on average 3.66(on the basis of 5) Relating to the items, the respondents' score of the sexual curiosity was 4.16, sexual behavior 3.85, self-consolation 3.81, aborthon 3.23, virgine 3.19, extramarital experiences 3.17. There were no significant differences in sexual knowledge between male and females, between younger and older students, residence, sexual experience, and how students mainly obtained their sexual knowledge. But a significant difference was also found religion in their sexual attitude(p<.005). In addition, results showed the relationship between sexual knowledge and sexual attitude(P=0.332).

콤포머의 NaOH 용액 내에서의 화학적 분해

박미란,양규호,최남기,이영준,김선미 大韓小兒齒科學會 2004 大韓小兒齒科學會誌 Vol.31 No.2

이상적인 수복재료는 치아의 저작기능과 심미성을 회복할 수 있어야 하며 동시에 생체 적합성과 구강 내 환경변화에 대한 내구성을 가지고 있어야 한다. 그러나 수복물이 장기간 구강 내 환경에 노출되면 수복물 표면의 마모와 변색 및 수복물 자체의 파괴가 발생될 수 있다. 콤포머도 복합 레진처럼 낮은 마모 저항성을 주요한 단점으로 지적할 수 있다. 본 연구에서는 현재 유치 수복에 많이 사용되고 있는 Dyract (Dentsply, USA), Elan(Kerr, USA), F-2000(3M, USA)의 3종의 콤포머(polyacid modified resin composites)를 알칼리성 용액(0.1N NaOH)에 보관하였을 때 각 제품의 무게손실, 분해층 깊이 및 시편내에서 용출된 Si, Al과 Ba의 농도를 평가하여 제품간의 분해저항성을 평가하고 비교를 하고자 하였다. 각 제품 당 6개의 시편을 제작하여 무게 측정을 한 후, 0.1N NaOH용액, 3㎖에 저장하여 60℃에서 보관하였다. 2주후 제거하여 1.23% HCI로 2시간동안 중화하고 증류수로 세척한 60℃에서 건조하였다. 분해저항성은 무게손실, 분해층 깊이 및 시편내의 Si, Al과 Ba의 농도 변화를 근거로 평가하여 다음과 같은 결과를 얻었다. 1. 각 제품의 무게 손실량은 1.43%~2.14%까지 다양하였으며 제품 간에 유의한 차이는 없었다. 2. 각 제품의 표면하 분해층 깊이는 182.92㎛~227.70㎛였으며 제품 간에 유의한 차이는 없었다. 3. 각 제품으로부터 용출된 Si, Al양은 각 제품마다 유의한 차이가 있었으며(p<0.05), Si는 Dyract에서 Al은 F-2000에서 가장 많은 양이 방출되었다. 용출된 Ba양은 각 제품마다 유의한 차이가 없었다. 4. 무게 손실량과 표면하 분해층 깊이 사이의 상관 관계에서 비교적 높은 상관관계를 보였다(r=0.64, p<0.05). 5. 주사전자현미경 관찰시 0.1N NaOH용액에 보관한 후 콤포머기질과 필러사이의 결합의 파괴를 관찰할 수 있었으며, 기질이 소실되어 필러입자가 두드러진 양상을 보였다. 또한 필러입자의 표면은 분해에 의해 탈회된 모습을 보였다. 이상의 결과에서 현재 유치 수복에 사용되고 있는 3종의 콤포머는 분해저항성에 있어 유의한 차이를 보이지 않았다. The aim of this study was to evaluate the resistance to degradation of three commercial compomers in an alkaline solution. Dyract(Dentsply). Elan(Kerr) and F-2000(3M) polyacid modified resin composites(compomers) were used in this study. The resistance to degradation was evaluated on the basis of mass loss(%), degradation depth(μm) and Si, Al, Ba loss(ppm). The results were as follows : 1. The mass loss of each brand was 1.42%-2.14% and there was no statistically significant difference of mass loss among Dyract, F2000 and Elan. 2. The degradation layer depth of each brand was 182.92-227.7μm and there was no statistically significant difference of degradation layer depth among Dyract, F2000 and Elan. 3. There was statistically significant differences in Si-loss and Al-loss among three compomers (p〈0.05). Si loss was the highest value in Dyract and Al loss was the highest value in F2000. 4. There was statistically significant correlation between mass loss and degradation layer depth (r=0.60, p〈0.05). 5. In SEM finding, there was some destruction of compomer matrix-filler interface in post-exposure specimen to NaOH solution. As the matrix decreased, the filler particles distinguished and the periphery of the filler particles appeared whitish color due to degradation.

Rhizopus oligosporus에 의한 Oncom제조

박성오,한영란,변정아 서울여자대학교 자연과학연구소 1991 자연과학연구논문집 Vol.2 No.-

Peanut presscake powder with 1% different starch sources (tapioca, corn, potato) was used for preparation of Oncom and inoculated with Rhizopus oligosporus and incubated at 30℃ for 48 hours. During incubation, it was analyzed every 12 hours for the determination of moisture, reducing sugar, soluble sugar, total sugar, crude lipid, pH, crude protein, soluble nitrogen and total amino acids, and, for mineral determination, final product was used. Oncom prepared with peanut presscake as control (group Ⅰ), 1% tapioca (group Ⅱ), 1% corn starch (group Ⅲ) and 1% potato starch (group Ⅳ) added to peanut presscake were determined. The results of 48 hours incubation product were as follows : the moisture content for group Ⅰ,Ⅱ,Ⅲ and Ⅳ were 55.84, 53.50, 54.47 and 58.86 (wt%), respectively and its content was increased compared with unfermented peanut presscake. Reducing sugar content were 0.98, 1.06, 0.78 and 0.60(wt%), respectively and soluble sugar content were 1.06, 0.89, 1.05 and 0.99(wt%), respectively and it was increased slightly compared with unfermented peanut presscake. Total sugar content were 5.01, 4.54, 2.66 and 5.16(wt%), respectively and it was decreased greatly compared with unfermented one. Crude lipid content were 7.24, 7.24, 7.08 and 6.94(wt%), respectively and it was decreased slightly. The value of pH were 6.38, 6.31, 6.20 and 6.22, respectively and it was increased compared with unfermented one. The crude protein content were 5.25, 4.97, 5.99 and 5.31(wt%), respectively and it was almost same as unfermented one. The soluble nitrogen content were 26.0, 23.0, 25.0 and 20.0(mg%), respectively and it was increased almost 7 times. Total amino acids content were 1404.0, 1468.4, 1342.4 and 1377.4(mg%), respectively and it was increased very slightly. The minerals (K, Na, Ca, Cu, Mg, Mn, Fe, Zn) contents were determinted. The content of total sugar, K, Na, Mg, Mn were higher in Ⅳ group than any other groups. The content of reducing sugar, crude lipid, total amino acids were higher in Ⅱ group than any other groups. The content of soluble sugar, crude lipid, soluble nitrogen were higher in Ⅰ group than other groups and the content of crude protein in group Ⅲ were higher than other groups.

외상성 골낭에 관한 연구

박원길,고재경,김영진,오경란 大韓口腔顎顔面放射線學會 1997 Imaging Science in Dentistry Vol.27 No.2

Traumatic bone cyst is a pathologic cavity that is not lined with epithelium. It is, therefore, not a true cyst. It may be a normal variant rather than a disease process. The etiology of the condition is unknown. This condition is occurred widely ranging ages(2 to 75years), however, most are found during the second decade of life. Radiographically, this condition is radiolucent lesion with well-defined outline, scalloping of superior margins. Cyst enucleation and curettage is the treatment of choice. The authors compared and analyzed the clinicoradiologic features of the five cases of traumatic bone cyst, diagnosed at the Dental college hospital in Chosun University, Kwangju, Korea., The five cases wear shown the followed results; 1. 3cases occurred in second decade of life & no significant sex differences (M:F, 2:3) All cases occurred in mandible. 2. Two patients complained symptoms, but three cases had no symptom with encountering during routine examination. 3. In 3 of 5cases, teeth vitality existed except one tooth and no checking of teeth vitality in two cases. 4. All cases didn't have any accurate trauma history, but one case was in orthodontic treatment, another case was postextraction site area. 5. Radiologically, "scalloping appearance" were evident in all cases; in 3 cases, multilocular tendency & only one case seen intact mandibular canal image. 6. Histologically, all section showed bone trabeculae with blastic activity, 2 cases showed no epithelial lining, and other 2 cases were seen innflammatory cell infiltration in edematous tissue. 7. Surgical intervention (curettage) was that treatment of choi

성견 절치에서 수직 골결손 형성후 함입이동시 치주조직의 반응

박양수,김영준,최홍란,황현식 전남대학교 치과대학 2000 전남치대논문집 Vol.12 No.1

The present study was performed to investigate the periodontal response following orthodontic intrusion of teeth with vertical bony defect produced by periodontal disease. Vertical bony defect and periodontal disease were produced adjacent to the both maxillary second lateral incisors in four adult dogs, weighing 20kg or more. Four weeks later, a flap operation was performed to eliminate inflammation and a reference notch was made on the root surface at the level of bottom of each defect. Two weeks after periodontal surgery, four weeks of intrusion and another four weeks of retention was executed on the right incisors while left incisors served as the control. Through the histologic analysis, following results were obtained. 1. The periodontal lesion which was produced by elastics and artificial bony defect was characterized by three walled infrabony pocket. 2. In the histologic section of control side, the base of the pocket was located below the level of the cemento-enamel junction. Connective tissue attachment was formed apically from the base of the pocket. 3. On the experimental side, the base of the pocket was localized close to the cemento-enamel junction. Connective tissue attachment was formed near to pocket base and extended apically. 4. New cementum was formed from the notch coronally toward the epithelial junction along the root surface in the both intruded incisors and control. 5. In the fluorescence microscopic observation, bone regeneration was began at the base of defect and the remodelling pattern characterized by a combination of coronal bone generation and resorption of the marginal crest could be detected. Although the remodelling pattern was the same in the both side, the bone remodelling in intruded incisors was continued during tooth movement. The results of the present study suggest that an orthodontic intrusion of extruded incisors with vertical bony defect can be carried out with a desirable periodontal response, provided that both the oral hygiene and the biomechanical force system are kept under control.

Methotrexate가 脾臟에 미치는 影響에 관한 硏究

朴景蘭,李永浩,金源式 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

In order to study the morphological changes of spleen after treatment with methotrexate, ICR mice were used as experimental animal. The experimental mice were divided into control, 50mg/kg and 500mg/kg of methotrexate treated groups. Methotrexate injected intravenously via tail vein once on the 8th day of pregancy. The mice were sacrificed on the 1st, 2nd 3rd and 4th day after treatment, the spleens were removed and fixed in 10% neutral buffered formalin solution for 48hrs. Spleens were cutted 5㎛ thick and stained with H-E, then observed under the light microscope. The results were as follows: 1. The size of spleen were reduced after MTX treatment more on dose day by day. 2. The periarterial lymphatic sheath (PALS) and germinal center were also reduced after MTX treatment. 3. The lymphocytes of white pulp showed nuclear pyknotic changes and many nuclear debris were found after MTX treatment. 4. The brown hemosiderin pigments were increased markedly in the red pulp of MTX treated groups. 5. Marked proliferation of fibrous trabeculae were found in the red pulp of MTX treated mice.

치근단 낭종에서 염증세포와 콜레스테롤 결정의 분포양상

박영식,최홍란 전남대학교 치과대학 1995 전남치대논문집 Vol.7 No.1

Apical periodontal cysts correlate to dental caries, and derived from the epithelial rests of apical granuloma. The lesions are histologically characterized by infiltrates of monocytes/macrophages, lymphocytes, plasma cells and mast cells. In addition, cholesterol crystals and foam cells are found. The aim of this study was to investigate the relationship between chronic inflammatory cells, cholesterol crystals and foam cells. The results obtained were as follows : 1. In all apical periodontal cysts, mild to severe number of inflammatory cells could be observed, while foam cells and cholesterol crystals were found in varying number. 2. The cholesterol crystals were not always associated with foam cells. 3. The cholesterol crystals appeared as long narow spaces with pointed tips and cluster formation. 4. The foam cells and choesterol crystals surrounded by lymphocytes, plasma cells, and erythrocytes, hemosiderin pigments and giant cells were observed. 5. Vast number of the cholesterol crystals and foam cells congregated in connective tissue, less frequently crystal mass eroded the epithelial lining and empties the crystal into the cystic cavity. From above the results, it was found that cholesterol crystals and foam cells are characteristic findings and correlated to inflammatory reaction in apical periodontal cyst. It suggested that inflammation closely related to cholesterol crystals and foam cells.

K市 管未 水道꼭지의 水質 特性에 關한 硏究

박란,이성기,송영일 조선대학교 환경공해연구소 1999 環境公害硏究 Vol.15 No.-

The purpose of this study was water quality of water treatment plants and distribution and service pipes examine and comparison, remain effect of disinfectant examine, used quality mark out prediction technique according to the distance apart from water treatment plants as intend to contributed water management. 1. The Langelier Saturation Index which is used to measure corrosion is influenced by the pH of the water. pH is at 6.3∼7.3 at the terminal place and in summer there is minimal pipe line corrosion in K city. 2. The shorter the distance from the water treatment plant to the tap the free chlorine residual concentration is only slightly deminished, but as the as the distance from source to tap increases the free chlorine residual concentration in the water decreases. Free chlorine residual change the 1st reaction, the 1st reaction was C = 0.70e (-0.105L). 3. The equation of 1st reaction law for color was C= 1.636e (0.008L), 1st reaction law for turbidity was C = 0.57e (0.050L). 4. The optimum distance for free chlorine residual concentration of 0.2 ㎎/L is about 15∼16km from water treatment p1ant(or distribution reservoir). Therefore, when the terminal place is 20∼25km the injection of free chlorine residual as the concentration of 0.3∼0.5 ㎎/L from water treatment plants(or distribution reservoir). The high free chlorine residual concentration avoid inject, in result pipe line corrosion and THM's was potential of dissolved.

간질환환자의 말초임파구 염색체에서 Fragile Site의 의의

박경란,김수일,이영호,김원식 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

To understand chromosomal instability in hepatitis B virus related disease and significance of fragile sites in hepatoma, lymphocytes of peripheral blood from patients of hepatitis, liver cirrhosis and hepatoma were cultured. Fragile sites of chromosomes were counted by chromosome group and the results were as follows. 1. The frequencies of fragile sites were significantly increased in hepatitis, liver cirrhosis and hepatoma compared with control group. 2. The frequency of fragile sites by chromosome group were appeared in chromosome C, A, B and E group in turn on control group, chromosome C, A and B in turn on hepatitis and liver cirrhosis, and chromosome C, B and A in turn on hepatoma respectively. 3. The frequency of fragile sites was significantly increased in liver cirrhosis with HBsAg( + ) in comparison to HBsAg(-) liver cirrhosis. However no differences of frequency were founded in hepatoma by presence of HBsAg. 4. The common specific fragile sites of chromosome were appeared in chromosome 2q13, 5q34, 6p22 and 11q13, It is presumed that chromosomal instability by hepatitis B virus may induce hepatocellular carcinoma, and the common fragile sites found in chromosome 2q13, 5q34, 6p22 and 11q13 would be correlated with sites of oncogenes. However, more study needs to he done in correlation between fragile sites and oncogenes.

비인두 암종에서 Epstein-Barr virus 감염과 p53 유전자 변이의 관계

박영식,최홍란 전남대학교 치과대학 2001 구강과학 Vol.13 No.1

Nasopharyngeal carcinoma is a common neoplasm of the head and neck, and commonly found in Southeast Asia. Epstein ­ Barr virus(EBV) is found within the malignant cells in most cases of nasopharyngeal carcinoma. The p53 gene has been implicated as a tumor suppressing gene in many types of human cancers, but the frequency of mutations varies in different cancers. To find out the correlation between EBV infection and p53 mutation in nasopharyngeal carcinoma, we evaluated the presence of EBV using EBER ­ 1 in situ hybridization and a mutation of p53, using PCR ­ SSCP analysis and DAN sequencing method. We examined nasopharyngeal specimens from 33 patients, histologicaly consisting of 27 cases of nonkeratinizing squamous cell carcinoma (WHO ­ Ⅱ) and 6 cases of undifferentiated carcinoma (WHO ­ Ⅲ) according to the World Health Organization claasification. EBER1 were detected in 14 of 33 (42.4%) cases. Positive hybridization signals were restricted to the nuclei of tumor cells. Of 14 cases 14 cases, 11 out of 27 (40.7%) cases of WHO ­ Ⅱ, and 3 out 6 (50%) cases of WHO ­ Ⅲ were positive. 33 biopsy specimens were analyzed by PCR ­ SSCP for p53 gene mutations. Our study concentrated on exons 5 through 9 of the p53 gene. However, we did not detect any mobility shifts n exon 5/6, 7 and 8/9 of the p5 gene in comparison to the banding patterns of the normal control. The reliability of the SSCP analysis was confirmed by sequencing five randomly chosen specimens for the exon 5 ­ 9 region. Among five cases, 4 out 5 cases were EBER1 positive and histologially WHO ­ Ⅱ, and 1 out of 5 cases were EBER1 negative and WHO ­ Ⅲ. We detected point mutation in 3 cases were EBER ­ 1 positive and WHO ­ Ⅱ. No alteration of the exon 7 and 8/9 of the p53 sequence was observed. Alteration occurred in 2 out of 3 cases; GCC was replaced by GTC at codon 131, resulting in a change in amino acid sequence from alanine to valine. In one 3 cases, TCC was replaced by TTC at codon 149, resulting in a change from arginine to arginine. Based on the above results, this study shows correlation between the presence of EBV and mutations of the p53 gene, suggesting contributions to malignant transformation in nasopharynx. We need to futher investigate this situation to understand the stepwise progression of nasopharyngeal carcinogenesis.