의류제품의 치수 규격동향

한상미,Han, Sang-Mi 기술표준원 2003 기술표준 Vol.15 No.-

채취 시기 및 지역에 따른 봉독의 성분 분석

한상미,윤형주,백하주,Han, Sang-Mi,Yoon, Hyung-Joo,Baek, Ha-Ju 한국응용곤충학회 2012 한국응용곤충학회지 Vol.51 No.3

서양종 꿀벌 독의 채집시기와 지역에 따른 봉독의 성분 변화 및 약리효과에 미치는 영향을 검토하였다. 채집시기는 5월부터 9월까지, 채집지역은 전국 35개 지역으로부터 채취한 봉독을 대상으로 2010년과 2011년 2년에 걸쳐 동일 지역에서 동일한 방법으로 봉독을 채취하였다. 채취한 봉독은 액체크로마토그래피를 통해 멜리틴과 아파민 그리고 포스포리파아제 A2의 성분 함량을 분석하였다. 그 결과 채집시기와 지역에 따른 성분에 유의한 차이는 확인되지 않았다(One way-ANOVA, Duncan's test (${\alpha}$=0.05)). 봉독의 성분은 채집시기와 지역에 관계없이 멜리틴 $55.2{\pm}2.07%$, 아파민 $2.57{\pm}0.103%$ 그리고 포스포리파아제 A2는 $12.51{\pm}0.37%$을 차지하였다. 이상의 결과로부터 봉독은 채취시기에 따른 주요 성분은 차이를 갖고 있지 않았으며, 이는 꿀벌의 먹이, 사육온도 등 외부 환경이 봉독 분비에 영향을 주지 않는 것으로 사료되었다. This study aims to investigate whether geographical variation affects the antibacterial component properties of honeybee (Apis mellifera L.) venom in Korea. Honeybee venom samples were collected from May to September, during 2010 and 2011, from 35 different sites, and were analyzed for major components, including melittin, apamin and phospholipase A2 were determined by a liquid chromatography using ammonium formate, acetonitrile, trifluoracetic acid. On average, melittin, apamin and phospholipase A2 were determined $55.2{\pm}2.07%$, $22.57{\pm}0.103%$, and $12.51{\pm}0.37%$, respectively. The ratio of the major components, including melittin, apamin and phospholipase A2 did not differ significantly according to flower or temperature during collections (One way-ANOVA, Duncan's test (${\alpha}$=0.05)).

국산 프로폴리스의 Helicobacter pyrori 억제효과

한상미,홍인표,우순옥,김세건,장혜리,장재선,Han, Sang Mi,Hong, In Phyo,Woo, Soon Ok,Kim, Se Gun,Jang, Hye Ri,Jang, Jae Seon 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.1

Propolis possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of this study was to evaluated chemical composition and in vitro anti-Helicobacter pylori activity of eight propolis ethanolic extracts form different region in Korea. The chemical composition of eight Korean propolis was investicated by absorption spectrophotomety and UHPLC. Total penolic and total flavonoids content ranged between $21{\pm}1.5$ and $31{\pm}1.8$ gallic acid g/100 g and $7.2{\pm}0.9$ and $24.4{\pm}1.9$ quercetin g/100 g, respectively. The p-coumaric acid as standard components content ranged between $0.29{\pm}0.00$ and $2.34{\pm}0.02mg/g$ using UHPLC. Cinnamic acid content between $0.43{\pm}0.00$ and $1.33{\pm}0.01mg/g$. 80% ethanolic extract of propolis against H. pylori was evaluated by using the disc diffusion method. Ethanol was used as a control. Mean diameters of H. pylori growth inhibition using $10{\mu}g/mL$, $50{\mu}g/mL$ or $100{\mu}g/mL$ ethanol propolis per disc were 0, ranging from 0.9 to 2.1 mm and from 1.9 to 3.5 mm, respectively. In conclusion, variation in the chemical composition of Korean propolis were mainly due to the difference in the preferred regional plants visited by honeybees. Also Korean propolis possesses considerable antibacterial activity against H. pylori. There was not confirmed a correlation between the chemical composion contens and anti-H. pylori effect. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.

호박벌 일벌독의 성분 분석 및 생리활성 탐색

한상미,이광길,여주홍,권해용,우순옥,윤형주,김미애,김원태,백하주,Han, Sang-Mi,Lee, Kwang-Gill,Yeo, Joo-Hong,Kweon, Hae-Yong,Woo, Soon-Ok,Yoon, Hyung-Joo,Kim, Me-Ae,Kim, Won-Tae,Baek, Ha-Ju 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.2

호박벌 일벌독의 성분과 생리활성을 규명하기 위하여 단백질 성분분석과 암세포 생육 저해 효능, 항균력을 검토하였다. 이차원단백질 분석을 통해 호박별의 일벌독은 63개의 단백질이 존재하는 것으로 확인되었으며, 가장 많은 함량을 보이는 3개의 단백질을 염기서열 분석하였다. 그러나 이들 성분은 아직 밝혀지지 않은 성분으로 판단되었다. 호박벌 일벌독의 암세포 (간암; Hep3B, 폐암; A549, 유방암; BT-20, 위암; AGS) 에 대한 생육 저해능은 시료 농도가 증가함에 따라 증가하는 경향을 보이며 100ng/ml에서 간암세포 (Hep3B) 에 대한 생육 저해능이 55%로 가장 높았다. 항균활성에 E. faecium 과 S. sonnei에 대하여 최소발육억제농도와 최소살균농도 모두 각각 0.256ug/ml 로 강한 항균활성을 나타내었으며, 그 외의 피검균에 대해서도 비교적 높은 활성을 보였다. 이상의 결과로부터 호박벌 일벌독의 성분은 다른 벌의 독성분과는 차이를 보이며, 그 생리활성에 있어 약리학적 이용이 가능할 것으로 판단되었다. Pure Bombus ignitus venom samples were submitted to two-dimensional gel electrophoresis. A total of 64 excised spots were analyzed by mass spectrometry. Three main proteins resulted in the identification have not been described in other bee venoms before. Dose-dependence against human carcinoma (Hep3B, BT-20, A549 and AGS) were observed from 1ng/ml to 100ng/ml. Expecially, the treatment of 100ng/ml B. ignitus venoms showed the highest cytotoxicity with 55% against hepatocellular carcinoma (Hep3B). The B. ignitus venoms showed strong antimicrobial activities against Enterococcus faecium and Shigella sonnei, and practically antimicrobial activity against the other microorganisms tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of E. faecium and S. sonnei, were 0.256ug/ml, respectively.

무당벌레(Harmonia axyridis) 추출물에 의한 BV-2 세포주의 Nitric Oxide 생성 저해 활성

한상미,이상한,윤치영,강석우,이광길,김익수,윤은영,이평재,김선여,황재삼,Han Sang-Mi,Lee Sang-Han,Yun Chi-Young,Kang Seok-Woo,Lee Kyung-Gill,Kim Ik-Soo,Yun Eun-Young,Lee Pyeong-Jae,Kim Sun-Yeou,Hwang Jae-Sam 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.1

퇴행성 뇌질환은 뇌에 존재하는 면역세포인 소교세포의 염증반응이 발병 요인 중의 하나로 알려져 있다. 이에 본 연구는 초고속자동화시스템(high throughput screening: HTS)을 이용하여 약용곤충추출물로부터 항산화와 항염증 기능이 있다고 알려진 무당벌레 추출물로부터 염증발생인자인 nitric oxide의 생성에 어떠한 영향을 주는지 다음과 같은 결과를 얻었다. 소교세포인 BV-2세포에 대한 무당벌레 추출물의 세포독성은 물과 메탄올, DMSO 추출물에서는 100 ng/ml 까지는 거의 없었으나 에탄올 추출물은 1 ng/ml에서도 세포독성이 있었다. 물과 메탄을 추출물(50 ng/ml)은 LPS로 활성화된 BV-2세포에서 $TNF-{\alpha}$와 $IL-1{\beta}$의 발생을 35-60% 가량 억제 하였다. LPS로 유도된 NO의 생성은 물과 메탄올 추출물을 처리했을 때 각각 55%, 76% 억제되었다. 또한, MeOH 추출물을 처리했을 경우 LPS에 의한 iNOS 발현 정도를 단백질 수준과 mRNA 수준에서 현저하게 억제시킴을 확인하였다. Inflammation in the brain has known to be associated with the development of a various neurologiacal diseases. The hallmark of neuro-inflammation is the activation of microglia, brain macrophage. Pro-inflammatory compounds including nitric oxide(NO) are the main cause of neuro-degenerative disease such as Alzheimer's disease. In the study, we examined whether Harmonia axyridis extracts inhibit the NO production by a direct method using Griess reagent, western blotting and by RT-PCR(Reverse Transcription-Polymerase Chain Reactionin) the gene expression of inducible nitric oxide synthase(iNOS). Distilled water$(H_2O)$ and methanol(MeOH) extracts of H. axyridis inhibited the protein expression of TNF-a(Tumor Necrosis Factor) and IL-6(Interleukin) in LPS (Lipopolysaccharide) stimulated BV-2 cells at the concentration of 100 ng/ml. Incubation of BV-2 cells with the extracts of $H_2O$ of MeOH inhibited the LPS induced NO and iNOS protein. And this inhibition of iNOS protein is concordant with the inhibition of iNOS mRNA expression. These data suggested that H. axyridis extracts may play a crucial role in inhibiting the NO production.

실천 현장에서 요구하는 신입 사회복지사의 자질에 관한 연구

한상미(Han, Sang-Mee),전진호(Jeon, Jin-Ho),김기덕(Kim, Gi-Duk) 한국지역사회복지학회 2008 한국지역사회복지학 Vol.0 No.25

논문 : 학문 목적 한국어 말하기 평가 연구 -대학 입학 전 과정을 중심으로-

한상미 ( Sang Mee Han ) 국제한국어교육학회 2009 한국어 교육 Vol.20 No.1

This paper presents a study on developing the speech test tools for learners of Korean for Academic purpose. The purpose of this study is to suggest a sample of useful proficiency test tool sets for evaluating communicative competence of the learners of Korean who prepare to enter Korean colleges. The research is composed of 4 chapters. The first chapter presents the purpose this study. The second chapter focuses on the theoretical background of the study, especially on the content and the assessment categories of the speech test tools, the task·function lists for academic purpose, and the proficiency level for studying in colleges. The third chapter is composed of three sub-chapters which show the assessment criteria, types of question forms for the speech test, and the samples of the test tool set. In the last chapter a brief summarization of this study is made and some suggestions are offered. (Yonsei University)

모바일 기반 한국어 동영상 교수자료 개발 원리 -한국어능력시험(TOPIK) 이해 영역 학습 동영상 콘텐츠개발 사례를 중심으로-

한상미 ( Sang Mee Han ),이복자 ( Bok Ja Lee ),김종인 ( Jong In Kim ),최유경 ( Yoo Kyung Choi ),김지혜 ( Ji Hye Kim ),박지원 ( Jee Won Park,),김선영 ( Seon Yeong Kim ) 연세대학교 언어연구교육원 한국어학당 2014 외국어로서의 한국어교육 Vol.41 No.-

The present study aims to present the principles of developing a video content for mobile-learning and to show the implementation process of the content, focusing on intermediate TOPIK comprehension section. This study reports four major principles of mobile content development; student-centeredness, motivation, input-efficiency, and authenticity. First, to keep the learning process student-centered, a variety of question types were formed for the learners to organize and decide the order and method of learning for themselves, while self-evaluation stage was set up for the learners to check what they have learned. Next, to build and constantly maintain the learners’ motivation and interest, learning strategies were constructed by the ARCS (Attention, Relevance, Confidence and Satisfaction) motivation theory. Also, strategic learning methods were invented in the input process to improve the learners’ inner cognitive processing ability. Lastly, all the texts were constructed to have authenticity in meaningful contexts. Under these principles, the video content consists of the following six stages; 1) learning objective 2) strategies for solving the question 3) applying the strategies 4) language learning strategy 5) expected questions 6) self-evaluation.

사회복지사의 감정노동이 소진에 미치는 영향 - 슈퍼비전과 인권의 조절효과를 중심으로 -

한상미 ( Han Sang-mee ),양성욱 ( Yang Seong-wook ) 한국자치행정학회 2018 한국자치행정학보 Vol.32 No.1

본 연구의 목적은 사회복지사의 감정노동이 소진에 영향을 미치는 과정에서 슈퍼비전과 인권이 조절기능을 하는지 검증하는 것이다. 이를 위해 국가인권위원회(2013)의 ‘사회복지사 인권상황 실태조사 보고서’에 사용된 전국 사회복지사를 대상으로 조사한 2,295명의 데이터를 활용하였다. 분석결과, 개인 지원에 초점을 두는 슈퍼비전은 유의미한 조절효과를 보이지 않았으며, 조직시스템에 기반 하는 인권 요인인 권리침해경험, 상사 및 동료의 폭력, 클라이언트 폭력은 모두 유의미한 조절효과가 나타났다. 연구결과, 사회복지사의 감정노동으로 인한 소진 완화를 위해서는 사회복지사들의 인권보호를 위한 조직시스템을 강화할 필요성이 제시된다. 연구결과를 바탕으로 사회복지사들의 인권강화를 위한 제도적 보완책으로 사회복지사들의 인권과 노동법 교육의 의무화, 사회복지사들의 안전권 확보를 위한 기관의 노력 강화, 감정노동자를 보호하는 구체적인 법안 마련 등의 실천적, 정책적 방안을 제시하였다. The purpose of this study was to examine the effect of emotional labor on burnout for social worker focused on moderating effect of supervision and human rights. For this, the data of 2,295 social workers on national wide which was used in The Report of Actual Situation Survey of Social Worker’s Human Rights by National Human Rights Commission of The Republic of Korea in 2013 were analyzed. The results showed that the supervision which focus on supporting individual were not significant moderating effect to social worker’s emotional labor lead to burnout, while factors related on human rights were all significant moderating effects. Factors related on human rights were included such as the experience of infringement of basic right, the experience of violence from peer and superior in the workplace and also from clients. According to the study, it is concluded that reducing the burnout caused by emotional labor could be achieved through strengthening organization system to protect social worker’s human rights. This study was meaningful to examine empirically how alleviate burnout caused by emotional labor, distinctively both by supporting individual social worker and by improving the system. This study suggested practical and political way to enhance human rights for social workers, including mandatory education on human rights and labor law for them, the enforcement of the duty for social work organization to improve worker’s safety rights and legislation specifically to protect the emotional labor.

요양병원 간호사의 의사소통 능력과 직무스트레스가 조직유효성에 미치는 영향

한상미(Han Sang-Mi),박성주(Park Sung-Ju) 전남대학교 간호과학연구소 2019 Nursing and Health Issues(NHI) Vol.24 No.2

Purpose: This study was a descriptive survey and aimed to identify the communication skill, job stress, and organizational effectiveness of geriatric hospital nurses and examine the effects of communication skill and job stress on organizational effectiveness. Methods: The participants were 180 nurses with more than one year of work experience as a geriatric hospital nurse, who were randomly recruited from six selected long-term care hospitals with more than 100 beds, located in G Metropolitan City and J Province. Results: Communication skill had an average score of 3.56 points, job stress had 3.77 points, and organizational effectiveness had 3.17 points on a five-point scale. Organizational effectiveness of long-term care hospital nurses had a significant positive correlation with communication skill (r=.43, p <.001) and a significant negative correlation with job stress (r=−.28, p <.001). The factors significantly influencing organizational effectiveness were communication skill and job stress. The explanatory power of the two variables was 23%. Conclusion: Organizational effectiveness had a significant positive correlation with communication skill and a significant negative correlation with job stress. Therefore, it is judged to be necessary to develop various studies and programs for enhancing communication skill and reducing job stress in order to promote organizational effectiveness of long-term care hospital nurses.