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여주홍,이광길,이용우 한국잠사학회 2001 한국잠사곤충학회지 Vol.43 No.1
The collagen growth effects in rats using B. mori fibroin sponge sheet, pure fibroin solution and chitosan sponge sheet were investigated. Histopathological inspection of the wound 12 days later showed the increase of a vascular ingrowth and the absence of inflammatory cells. Wound healing effects were accelerated in the order of Fibroin sponge sheet ≥Fibroin dressing solution > Chitosan sponge sheet > Control. The mean percentage of collagen production in rats after 12 days was 46.12±4.58% and 42.11±5.67% in fibroin sponge sheet and dressing solution-treated rats, respectively. These values were higher than the relative content in the chitosan sponge sheet and control groups, which was 23.87±3.24 and 2.42±0.32%, respectively.
Calcium chloride 피브로인 용해물의 Gel Filtration Chromatography에 의한 순수분리 및 효소 가수분해 효과
여주홍,이광길,이용우 한국잠사학회 1999 한국잠사곤충학회지 Vol.41 No.3
The pure-separation of calcium chloride-treated fibroin hydrolysates could be carried out using gel filtration chromatography. Also, the effect of its enzymatic hydrolysis was investigated in order to find out the enhancement of their functionality. The average molecular weight(Mw), solubility and free amino acid compositions of three hydrolysates samples (calcium chloride, calcium chloride-flavo-urzyme and calcium chloride-thermoase) were measured to compare their characteristics. The molecular weight of calcium chloride hydrolysate was about Mw 46,800 and it can be reduced to Mw12,500 and 1,070 upon the enzymatic hydrolysis by flavourzyme and thermoase, respectively. A solubility of calcium chloride-treated samples shows about 60% while calcium chloride/enzyme-treated samples are perfectly soluble (100% solubility). The total amino acid composition of calcium chloride enzymatic hydrolysates are much higher than that of calcium chloride hydrolysate.
쌍극자-쌍극자 상호작용에 의한 배향섬유의 분자축 각도 결정 - Polyamide계 섬유에의 응용 -
여주홍,이인전,Yeo, Ju-Hong,Lee, In-Jeon 한국섬유공학회 1997 한국섬유공학회지 Vol.34 No.1
The bond direction between fiber axis and N-H bond direction of $^15N$-Gly labeled silk fibroin fiber and poly-p-phenyleneterephthalamide(PPTA) oriented were obtained from the dipolar-bipolar interaction information, respectively. Theoretical method for the determination of molecular bond directions relative to the axis of fiber was derived on the basis of solid state nuclear magnetic resonance dipolar-dipolar interaction tensor. The oriented samples were placed parallel to the applied magnetic field, and the $^15N-^1H$ hetero-nuclear dipolar-dipolar splitting values were obtained. Substituting this values into the derived equation, the N-H bond angles ($$\theta$_NH$=83$^{\circ}$ and 97$^{\circ}$) are calculated. Also, the N-Hbond direcyions to fiber axis of PPTA are 44$^{\circ}$ and 67- These obtained values compared with the values from the angular dependation spectra simulation of oriented PPTA sample.
견사와 폐 우유팩으로부터 분리한 셀룰로오스가 함유된 복합 위생포 제작
여주홍,이광길,이용우,김종호 한국잠사학회 2000 한국잠사곤충학회지 Vol.42 No.2
The preparation of skin care gauze could be make to mixing separated cellulose from waste milk park and degummed silk fibroin fiber. Also, its wound covering and anti-barterial activity were investigated in order to find cut the enhancement of their functionality. By the 30% silk fibroin fiber including skin care gauze. the anti-bacterial activity values of Sraphylococcus stran are much 4 times higher than of0~10% silk fibroin fiber including skin care gauze. The average yield of cellulose from waste milk pack was obtalned 50-6O%, and their morphologie,, physical properties, modulus and biodegradation ratios are studies, respectively.
황색 형광견 중 Tyrosine잔기 거동의 분광학적 분석
여주홍,이인전,한명세 한국잠사학회 1997 한국잠사곤충학회지 Vol.39 No.2
The behavior of tyrosine(Tyr.) residue of Bombyx mori silk fiber from yellow fluorescent cocoon has been examined for the dependence of pH in aqueous silk solution under the presence of orange II salt. Through the peak separation of angular dependence of spectral pattern of 15N-Tyr. and (1-13C)-Tyr. between the fiber axis and the molecular bond direction, N-H bond in fiber as well as the orientation distribution around the fiber axis were analyzed. Also, and sericin component was obtained from these angular dependence of oriented spectral pattern. The pH dependence of the 13C NMR chemical shift of B. mori silk fibroin was examined in aqueous solution in the presence of orange II hydrophobic interaction such as stacking between the Tyr.-ring and the orange ll. All peaks of orange II are broad at pH ≥ 7.0. However, these become sharper at pH ≥8.0 and remain sharp at higher pH. In these higher pH range, a chemical shift change occurs due to the deprotonation of the Tyr. side group of fibroin. At higher pH, such a hydrophobic cluster is destroyed because of the electrostatic interaction according to the deprotonation of the Tyr.-OH group.
실크피브로인을 처리한 MC3T3-E1 조골세포 조건배양액의 파골세포 분화억제효과
여주홍(Joo-Hong Yeo),박경호(Kyung-Ho Park),주원철(Won-Chul Ju),이진아(Jinah Lee),이광길(Kwang-Gill Lee),우순옥(Soon-Ok Woo),한상미(Sang-Mi Han),권해용(HaeYong Kweon),김성수(Sung-Su Kim),조윤희(Yunhi Cho) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.8
본 연구는 MC3T3-E1 조골전구세포에 실크피브로인 처리 시 파골세포분화 관련 국소인자 중 파골세포로의 분화를 강력히 유도하는 IL-1β의 발현 및 파골세포로의 분화억제에 관여하는 국소인자인 OPG의 발현변화를 조골세포 조건배양액에서 살펴보았으며, 이 조골세포 조건배양액을 RAW264.7 파골전구세포에 처리 시 파골세포로의 분화를 억제하는지를 알아보았다. 조골세포 조건배양액에서 OPG의 발현은 농도별(0.001 ㎎/mL~0.1 ㎎/mL) 실크피브로인 처리 시 86%~100%로 양성대조군(+C)과 유의적 차이가 없었다. 그러나 IL-1β의 발현은 0.1 ㎎/mL 실크피브로인 처리 시 양성대조군(+C)의 1.8% 수준으로 그 발현을 현저히 억제 하였다. 조골세포조건배양액을 처리한 RAW264.7 세포의 파골세포로의 분화정도는 농도 의존적으로 현저히 감소하였다. 특히, 0.1 ㎎/mL의 실크피브로인을 처리한 조골세포 조건배양액 처리 시 파골세포로 분화하지 않은 음성대조군(-RANKL) 수준으로 파골세포 분화를 현저히 억제한 것은 OPG의 발현 증가가 아닌 IL-1β의 발현 억제에 의한 것으로 사료되며, 추후 연구를 통해 관련 기전의 연구가 필요할것으로 생각되어진다. In this study, we investigated the indirect effect of silk-fibroin on osteoclastic differentiation of RAW264.7 cells. The conditioned medium were collected from MC3T3-E1 osbeoblasts treated with 0.001 ㎎/mL~0.1 ㎎/mL silk fibroin for 6 days, mixed in 1:1 ratio with osteoclast medium, and then added into RAW264.7 cells with receptor activator of nuclear factor kappa B ligand (RANKL), a differentiation inducer for 3 days. Of osteoclastic cytokines in the conditioned medium, the protein expression of osteoprotegerin (OPG) with silk-fibroin was not significantly different. However, the protein expression of interleukin (IL)-1β was specifically lower in a dose dependent manner. In RAW264.7 cells, the conditioned medium with silk-fibroin inhibited RANKL induced osteoclastic differentiation as total number of multinucleated tartrate-resistant alkaline phosphatase (TRAP)-positive osteoclasts in a dose dependent manner. Taken together, we demonstrated that the conditioned medium of silk-fibroin treated osteoblasts inhibits RANKL induced differentiation of osteoclasts with inhibiting selective expression of IL-1β.