누리논단 - '계란 살충제' 사태를 지켜보며… '축산물 생산단계 안전과 위생' 잡을 수 있습니다!

최농훈,Choe, Nong-Hun 축산물품질평가원 2017 KAPE누리 Vol.253 No.-

랫드 궁둥신경에서 155 kd Neurofascin의 발현개시에 대한 면역세포화학적연구

원희영,조익현,이종환,남상섭,최농훈,박재형,장병준,Won, Hee-Young,Cho, Ik-Hyun,Lee, Jong-Hwan,Nahm, Sang-Soep,Choe, Nong-Hoon,Park, Jae-Hyung,Chang, Byung-Joon 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.3

Neurofascin, one of the members of L1CAM, has been known to have some important roles during the development of nerve fibers. In order to investigate the role of neurofascin associated with the development of nerve fibers in the rat sciatic nerve, the initial development of NF155 in the paranode was studied with immuno-fluorescence and immuno-electron microscopy. The result of the present study showed NF155 was not detected in the fetal sciatic nerve and began to reveal at the postnatal day 0 (P0) and dramatically increased by time lapse until postnatal day 7 (P7). NF155 was prominently localized in the axolemma of paranode and not detected in the central region of node of Ranvier. According to the present study, NF155 is likely to have some relationships with the formation of paranode and myelin sheath.

천식유발 마우스에서의 폐 내 세포조성 변화와 IL-4 및 IL-10의 발현 양상

이수진,박세종,리천주,장양호,최농훈,Lee, Soo-Jin,Park, Se-Jong,Li, Tian-Zhu,Jang, Yang-Ho,Choe, Nong-Hoon 한국생명과학회 2006 생명과학회지 Vol.16 No.5

본 연구진은 일차로 BALB/C 마우스를 이용하여 항원으로 ovalbumin을 사용하여 천식을 유발하여 폐 내 세포구성에 미치는 영향을 다른 실험방법에 의해 만들어진 천식모델과 비교하여 살펴보았고 이차로 천식 발생에 중요한 역할을 하는 것으로 알려진 IL-4와 IL-10 knock out (KO) 마우스를 이용하여 천식모델을 구축하여 천식인자를 가진 개체에서의 반응과 천식관련인자가 결핍된 개체에서의 반응성의 차이를 살펴보았다. 천식의 유발은 실험 1일째 $20\;{\mu}g$ ovalbumin으로 감작시킨 후 실험 14일에 재감작을 시켰다. 그 후 nebulizer를 이용하여 nasal inhalation을 28일, 29일, 30일에 실시하여 천식을 유발시켰다. 천식 유발의 확인은 기관지폐포 세척술로 채취한 폐 내 세포액을 이용하여 총 세포수 및 염증세포의 증가와 폐 내 세포와 폐조직의 염색을 통해 분포율을 확인하였다. 천식의 발병 과정에서 IgE 관련 과민반응을 주도하는 IL-4와 Th2 세포의 기능을 억제한다고 알려진 IL-10의 면역화학염색을 통해 그 발현정도를 관찰하였다. BALB/c 마우스의 천식유발군의 경우 천식의 특징인 염증세포의 증가와 호산구의 증가와 세기관지 주위 염증세포의 침윤 및 기도 상피의 비후를 관찰할 수 있었다. 따라서 본 실험에 응용된 천식유발 방법은 적합하였으며 천식유발 절차가 성공적으로 수행되었음을 확인하였다. IL-4 및 IL-10 KO 마우스를 이용한 천식유발군의 경우 BALB/c 마우스를 이용한 천식유발군보다 호산구의 증가 정도가 미약하게 관찰되었고, 폐조직 내 염증세포의 침윤 정도도 감소하였다. 천식매개인자인 IL-4와 IL-10의 면역조직화학염색 결과에서도 양성반응이 거의 나타나지 않았다. 본 연구 결과, IL-10이 IL-4와 같이 천식과 관련하여 세기관지 염증 반응을 증가시키는 역할을 하는 것으로 생각된다. Asthma is a chronic inflammatory disorder of the airways, which characterized by bronchial hyperresponsiveness, reversible airflow limitation and respiratory symptoms. Internationally, the prevalence of asthma has been increased over last 3 decades. Recently, several studies of asthma have been reported with gradually increasing importance. To tesify the hypothesis that interleukin (IL)-4 and IL-10 may be an important determinant of the severity of airway inflammation, their expression was studied in mouse model of asthma. BALB/c mouse, IL-4 Knockout (KO) mouse and IL-10 KO mouse were sensitized with intraperitoneal injection of ovalbumin adsorbed to aluminum potassium sulfate, followed by challenges with intranasal ovalbumin on 3 consecutive days. The severity of pulmonary inflammation was assessed by eosinophilia in BAL fluid, number of total BAL cells, histopathological changes in lung tissues, and immunohistochemical staining against IL-4 and IL-10. In BAL fluid, the number of total cells was significantly increased in asthma induced mouse compare to the control. In asthma induced mouse, eosinophil was increased to 56% and neutrophil was 0.2%. In H &E stains, eosinophilic infiltration and epithelium hyperplasia were clearly noticed in asthma induced mouse. In immunohistochemical staining for IL-4 and IL-10, there was no positive reaction in control group. However, very strong reactions were appeared in asthma induced group. In this research, IL-4 and IL-10, which seem to play a central role in allergic asthma, KO mouse was utilized to test the causative relationship between airway inflammation and role of specific cytokine. Asthma induced IL-4 and IL-10 KO mice showed much decreased inflammatory reactions in the number of total BAL cells, in eosinophilic infiltration, and in immunohistochemical stains against diverse inflammatory proteins. These results suggest that IL-4 and IL-10 increase the asthmatic reactions in vivo mice model.

Foods Derived from Cloned Animals and Management Policies in Worldwide

이수진,장양호,김효비,이명헌,소병재,양병철,강종구,최농훈 한국축산식품학회 2012 한국축산식품학회지 Vol.32 No.4

Cloned animals are a result of asexual reproduction of animals using somatic cell nuclear transfer. Ever since the first report of a cloned sheep 'Dolly' produced by SCNT, increasing numbers of livestock, such as bovine and swine clones, have been generated worldwide. Foods derived from cloned animals have not been produced yet. However, the food safety of cloned animals has provoked controversy. The EU Food Safety Authority and U.S. Food and Drug Administration announced that milk and meat from cloned and non-cloned animals have no difference regarding food safety. However, food derived from cloned animals is considered unsuitable for eating vaguely. Moreover, there were scant information about cloned animals in Korea. Therefore, we surveyed the number of cloned animals worldwide including Korea and summarized the reports for cloned animals and discussed predictable problems.

Active biotelemetry를 이용한 젖소의 스트레스 반응 측정: II. 수송 스트레스 감소를 위한 수액투여 효과

이수한,이병한,임좌진,김진영,이동희,김재경,최농훈,정순욱,정병현,Lee, Su-han,Lee, Byeong-han,Lim, Joa-jin,Kim, Jin-young,Lee, Dong-hee,Kim, Jae-kyung,Choe, Nong-hoon,Jeong, Soon-wuk,Chung, Byung-hyun 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

A transport stress is one of the main causes of economic losses and physiological dysfunction. The present study has been performed to suggest a method to decrease the adverse effects above mentioned from transport. The groups were prepared as follows; (1) Control group : 4 cattle transported for 5 hrs (274 km) without any treatment, (2) Treatment group : 4 cattle treated with electrolyte-mineral solution (I.V.) at 1 hr before the enrollment of transport under same experimental condition with Control group. The blood specimens were collected at 1 hr before transport, 2.3 hrs (135 km) and 5 hrs (274 km) after the enrollment of transport, and 1, 6 and 18 hrs after fulfillment of transport. The collected blood specimens were analyzed for cortisol and epinephrine. Core temperature and heart rate were measured with active biotelemetry in every 30 minutes from 0.5 hr before the start of transport to 18 hrs after the end of transport. In results, the level of cortisol considerably increased to the peak either in Control group ($5.3{\pm}1.3{\mu}g/d{\ell}$) and in Treatment group ($4.0{\pm}2.6{\mu}g/d{\ell}$) at 2.3 hrs in transport. The concentration of epinephrine of Treatment group had been higher than that of Control group from the start of transport to 18 hrs after the fulfillment of transport. Particularly there was the biggest gap between Control and Treatment groups, $424.0{\pm}194.1pg/m{\ell}$ and $209.1{\pm}65.1pg/m{\ell}$ respectively, at 6 hrs after the end of transport. The heart rates were considerably increased either in Control group ($81.5{\pm}18.5$ to $126.3{\pm}7.8beats/min$) and in Treatment group ($114.3{\pm}14.4$ to $140.8{\pm}22.4beats/min$) with the enrollment of transport. These results indicate that the concentration of cortisol and the heart rate were pertinent to cognitive parameters to evaluate physiological responses against stress such as transport. In addition, the intravenous administration of electrolyte-mineral solution could be suggested as the method to decrease the adverse effects from a transport stress.

축산분야 소독제 정보 제공 스마트폰 어플리케이션 개발

정한성,김수권,최광훈,박지헌,쇠린택,최농훈,Chung, Hansung,Kim, Su kwon,Choi, Kwanghoon,Park, Ji Heon,Htet, Swe Lynn,Choe, Nonghoon 대한수의학회 2019 大韓獸醫學會誌 Vol.59 No.2

It is important to select the correct disinfectants and to use them appropriately in order to prevent the initial spread of highly infectious livestock disease, such as foot-and-mouth disease or highly pathogenic avian influenza. This study describes a smartphone application developed to enable livestock workers to observe information related to disinfectants for the prevention of livestock disease in the domestic market, regardless of time and location, through a Linux-based Android mobile platform. This application (Konkuk-Disinfectant Information Database) provides information on disinfectant names, components, license and use; it was designed to enable the user to share disinfectant information through social media.

Efficacy evaluation of commercial disinfectants by using Salmonella enterica serovar Typhimurium as a test organism

장영호,이광직,윤선종,이명헌,송재영,장병준,최농훈 대한수의학회 2017 Journal of Veterinary Science Vol.18 No.2

The efficacies of six commercial disinfectants were evaluated by using Salmonella enterica serovar Typhimurium under simulated natural conditions such as sub-zero temperature, short disinfecting time, and surface type (uneven or smooth). We used a suspensionmodel test to determine the disinfecting efficacy under varying contact times (1, 5, 10, and 30 min) and temperatures (25oC, 4oC, 0oC, and −10oC). The bactericidal effect according to surface structure was measured by using a carriermodel test at 25oC and −10oC. The effective concentrations of each disinfectant were fixed to give a disinfecting effect within a short time (< 1 min) at 25oC and −10oC. The suspension model results revealed that bactericidal efficacy significantly dropped at low temperature for most of the disinfectants used; a sodium dichloroisocyanurate product showed the strongest efficacy. In the carrier test, bacterial load on a wooden surface was more difficult to remove than that on a stainless-steel surface. The results show that commercial disinfectant products vary in their disinfecting efficacy, which is affected by several field factors including temperature, contact time, and carrier material. Environmental conditions and surface type for disinfection should be considered prior to selecting an optimal disinfectant in the field.

국내 흑염소에서의 소결핵, 브루셀라, Q fever 유병률 연구

김효비,김성준,김기나,김별,장병준,최농훈,Kim, Hyobi,Kim, Seongjoon,Kim, Kina,Kim, Byeol,Chang, Byungjoon,Choe, Nong-Hoon 대한수의학회 2016 大韓獸醫學會誌 Vol.56 No.4

As the meat of black goats has become popular as a healthy food, domestic goat meat-related industries are steadily growing. However, previous studies are scarce of informations about the zoonotic disease originated from the black goat in Korea. In this study, we investigated Korean black goat's infectious diseases representing bovine tuberculosis, brucellosis, and Q fever. One hundred and eighty samples were collected from a local slaughter house located in Jeollanam-do. Three typical zoonotic diseases were separately examined by carrying out enzyme linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Histopathological test was additionally performed in tuberculosis. In case of tuberculosis, results of the PCR and histopathological test were negative but the ELISA results were positive in eight samples. In case of brucellosis, one out of the total samples was shown to be positive in the ELISA and none in the PCR. In case of Q fever, there were forty one positive in the ELISA and twenty positive in the real-time PCR. Those results indicate that the Korean black goat could be a natural reservoir in the possible chain-infections among human, cows and goats. Thus, further study needs in order to improve productivity as well as to prevent the zoonosis spreading and circulation of other livestock with the black goat in this country.

랫드 수초좌골신경섬유에서 Neurofascin분포에 대한 면역세포화학적 연구

장병화,유관희,이종환,조익현,배춘식,박창현,한정미,최농훈,장병준,Chang, Byung-Hwa,You, Kwan-Hee,Lee, Jong-Hwan,Cho, Ik-Hyun,Bae, Chun-Sik,Park, Chang-Hyun,Han, Jeong-Mi,Choe, Nong-Hoon,Chang, Byung-Joon 한국현미경학회 2006 Applied microscopy Vol.36 No.2

Neurofascin은 L1CAM의 하나로 신경섬유의 발달과정에서 중요한 역할을 하는 것으로 알려져 있다. 말초신경의 수초형성과 관련된 neurofascin의 역할을 알아볼 목적으로 면역형광염색과 면역전자현미경기법을 이용하여 랫드의 수초좌골신경섬유에서 neurofascin의 분포를 추구하여 다음과 같은 결과를 얻었다. 1.수초형성이 진행됨에 따라 좌골신경섬유에서 neurofascin 분포는 매우 심하게 변화되었다. 2. 수초신경섬유에서 neurofascin은 Ranvier마디에서 약하게 국재하였다. 3. Neurofascin은 수초신경섬유의 paranodal loop, Schmidt-Lantermann incisure, 속축삭사이막, 바깥축삭사이막처럼 Schwann세포의 막이 밀착되지 않은 부위에서도 뚜렷하게 국재하였다. 이상의 연구결과로 neurofascin은 Schwann세포의 마주보는 막사이에 이상적인 간격을 유지하는데 어떤 역할을 하는 것으로 생각되며, 수초층에서 물질이동이 가능하게 하는 것으로 보인다. Neurofascin, one of the members of L1CAM, has been known to have some important roles during the development of nerve fibers. In order to investigate the role of neurofascin associated with the myelination of peripheral nerves, the localization of neurofascin in myelinated rat sciatic nerve fibers was studied with the immuno-fluorescence and immune-electron microscopy and the results are as follows; 1. According to the myelination is going on, neurofascin localization was dramatically changed in the sciatic nerve fibers. 2. In the myelinated fibers, neurofascin was weakly localized along the axolemma at the node of Ranvier. 3. Neurofascin was also apparantly localized at the non-compact area of Schwann cell membrane such as paranodal loop, Schmidt-Lantermann incisure, inner & outer mesaxons in the myelinated fibers. From the above results, neurofascin is likely to have a role to sustain the ideal gap of apposing membranes of Schwann cell, so it may enable to materials transport in the myelin sheath.

Silk fibroin hydrolysate ameliorates diabetic dyslipidemia in type 2 diabetic model mice

박준홍,Herry Jung,남윤이,김진봉,최농훈,서준교 한국통합생물학회 2013 Animal cells and systems Vol.17 No.5

The antidiabetic properties in silkmaterials, such as silkwormand silk fibroin, have been described in traditional folk medicine. In a previous study, we reported that the silk fibroin hydrolysate (SFH) had a protective effect against glucotoxicity in HIT-15 cells and an antidiabetic effect in a noninsulin-dependent diabetes mellitus (type 2 diabetes, T2DM)mouse model. This study aimed to determine whether SFH ameliorates diabetic dyslipidemia and promotes pancreatic β-cell function in a T2DM animal model (C57BL/KsJdb/db). SFH significantly improved blood glucose levels after 4 weeks of treatment. T2DM animals had a normal serum blood glucose level after 6 weeks of SFH treatment. Hemoglobin A1c (HbA1c) levels were significantly lower in T2DM animals that were treated with SFH for 6 weeks. Glucose tolerance in T2DM animals also significantly improved after 6 weeks of SFH treatment. Furthermore, plasma total cholesterol, high-density lipoprotein cholesterol, nonesterified fatty acid, and triglyceride concentrations significantly decreased in SFH-treated T2DM animals compared to T2DM animals. This result showed that SFH ameliorated diabetic dyslipidemia in the T2DM model mice. Taken together, our results suggest that SFH could be used as a potential agent to treat dyslipidemia in patients who have type 2 diabetes mellitus.