Human Umbilical Cord Blood Mononuclear Cell Transplantation in Rats with Intrinsic Sphincter Deficiency

임좌진,장진범,김지영,문성환,이정노,이경진 대한의학회 2010 Journal of Korean medical science Vol.25 No.5

To evaluate the effectiveness of the human umbilical cord blood (HUCB) transplantation for the treatment of intrinsic sphincter deficiency (ISD), we analyzed the short term effects of HUCB mononuclear cell transplantation in rats with induced-ISD. ISD was induced in rats by electro-cauterization of periurethral soft tissue with HUCB mononuclear cell injection after 1 week. The sphincter function measured by mean leak point pressure was significantly improved in the experimental group compared to the control group at 4 weeks. (91.75±18.99 mmHg vs. 65.02±22.09 mmHg,P=0.001). Histologically, the sphincter muscle was restored without damage while in the control group it appeared markedly disrupted with atrophic muscle layers and collagen deposit. We identified injected HUCB cells in the tissue sections by Di-I signal and Prussian blue staining. HUCB mononuclear cell injection significantly improved urethral sphincter function, suggesting its potential efficacy in the treatment of ISD.

Active biotelemetry를 이용한 젖소의 스트레스 반응 측정 I.보정 스트레스에 대한 경산우와 미경산우의 생체반응의 변화

임좌진,이병한,김진영,이수한,이동희,김재경,정순욱,최농훈,정병현,Lim, Joa-jin,Lee, Byeong-han,Kim, Jin-young,Lee, Su-han,Lee, Dong-hee,Kim, Jae-kyung,Jeong, Soon-wuk,Choe, Nong-hoon,Chung, Byung-hyun 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

This study was carried out to investigate the effectiveness of active biotelemetry to monitor stress response, and the effects of acute stressor (raising a hind foot of a standing animal) on body temperature (BT) and heart rate (HR) in comparison heifer group (n=4) with cow group (n=3). Both parameters were recorded by active telemetry. Cortisol assay was used to compare the result of active biotelemetry. The stressor consisted in restraining the cows for hoof treatments. Body temperature and heart rate were measured at 0, 10, 20, 30, 40, 50, 60, 90, 120, 180 and 360 minutes during the experiments, and also cortisol assay at 0, 0.5, 1, 2, 3, 12 and 24 hours. Base line for these animals was set in there accustomed environment at -24hrs and -30 mins before the beginning of experiments. The results obtained in this study were as follows: 1. In the BT, heifer group showed no significant difference from cow group, but were observed slightly higher BT than cow group during restraint stress for 30 minutes. 2. The both groups showed directly significant increase of HR immediately after the beginning of the restraint stress (P<0.01), but in the required time for the recovery of HR after finishing the stress, heifer group was 40 minutes and cow group 60 minutes. 3. In the cortisol assay, the both groups increased from 30 to 120 minutes after the beginning of experiment (i.e, from 0 to 90 minutes after finishing the restraint stress), showing significant difference from before the beginning of the stress (P<0.01), but heifer group showed great increase of the cortisol level from 30 to 60 minutes after the beginning of the stress, while cow group was observed at 60 minutes. In conclusion, we suppose that care is required to decrease psychological stress in cattle when they suffer from a series of procedures for diagnosis and treatment, especially in heifers rather than in cows. Also BT and HR obtained by active biotelemetry are reliable indicators of stress in cattle.

CLA와 selenium의 투여가 닭의 체구성과 혈중 콜레스테롤 수준에 미치는 영향

전호진,김진영,임좌진,이수한,이병한,정순욱,박희명,정병현,Jun, Ho-Jin,Kim, Jin-Young,Lim, Joa-Jin,Lee, Soo-Han,Lee, Byeong-Han,Jeong, Soon-Wuk,Park, Hee-Myung,Chung, Byung-Hyun 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.3

The purpose of this study was to examine whether conjugated linoleic acid and selenium supplementation in broiler chicken diets would be effective, enhance indices of immune status and body weight, and modulate serum lipid concentration. Forty Hyline brown chickens, 1 weeks of age, were divided into 5 groups of 8 chickens. Chickens were fed the experimental diets supplemented with 1% CLA (conjugated linoleic acid; Group 1), 1% CLA + selenium (Group 2), 1% safflower-seed-oil as LA (Group 3), 1% safflower-seed-oil as LA + selenium (Group 4) or nothing (Control) for 4 weeks. After 4 weeks, serum, liver, spleen and abdominal fat were taken. Measurement of total immunoglobulin were executed using sandwich ELISA. Weight ratio of liver to body showed that the group fed with CLA were significantly higher than the group fed with CLA + selenium. Weight ratios of spleen and fat to body showed no significantly differences. In concentrations of serum total cholesterol and HDL-cholesterol, the group fed with CLA showed significantly higher values than that fed with CLA + selenium. In concentrations of serum triglyceride and LDL-cholesterol there were no significantly differences between the treatment groups. In conclusion, supplementation of CLA with selenium protected hepatomegaly and reduced level of serum total cholesterol and HDL-cholestererol in chickens.

Active biotelemetry를 이용한 젖소의 보정 스트레스 반응 측정

이동희,이병한,임좌진,김진영,박희명,정병현,Lee, Dong-Hee,Lee, Byeong-Han,Lim, Joa-Jin,Kim, Jin-Young,Park, Hee-Myung,Chung, Byung-Hyun 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2

This study was conducted to investigate the influences of the rope and the tipping chute restraints on body temperature (BT) and heart rate (HR) as acute response for stress caused by restraining for diagnasis and treatment in cattle. Both parameters were recorded by active biotelemetry. In addition cortisol concentration in blood was analyzed as a indicator for stress response. Twelve cattle were divided into two groups based on hydraulic power, the rope restraint group and the tipping chute restraint group. BT and HR were measured at -30 (base), 0, 10, 20, 30, 40, 50, 60, 90, 120, and 180 minutes, including restraint period from 0 to 30 minutes during the experiment. The results obtained in this study was summarized as follows: 1. BT of the rope restraint group was increased ($39.8{\pm}0.3^{\circ}C$) until 20 min after restraint stress for 30 min, and then maintained with high values to the end of experiment. In the tipping chute restraint group, the BT was increased ($39.6{\pm}0.3^{\circ}C$) until the end of the restraint period, but then showed decrese until the end of experiment. 2. HR of both groups was maximized at the beginning of the restraint stress (P<0.05), and then it was decreased gradually but in the tipping chute restraint group showed increase again at the end of the reatraint stress (P<0.05). 3. The cortisol level of the rope restraint group was increased significantly ($9.72{\pm}5.09{\mu}g/d{\ell}$) until 30 min after the end of the restraint stress (P<0.05) and then decreased, but in the tipping chute restraint group showed great increase ($4.68{\pm}1.56{\mu}g/d{\ell}$) at the end of the restraint stress (P<0.05) and then decreased while the tipping chute restraint group was significantly lower than the rope restraint group 30 min after the restraint stress (P<0.05). In conclusion, this study suggests that the tipping chute restraint produces less response to physical stress than the rope restraint but the time for diagnasis and treatment should be shortened when using the tipping chute restraint.

쓰레기 매립지로부터 발생된 오염이 동물 림프구의 DNA 이상에 미치는 영향

김재우,이병한,임좌진,이수한,배춘식,김진영,정순욱,박희명,정병현 한국임상수의학회 2005 한국임상수의학회지 Vol.22 No.1

Lots of waste are produced from cities and embeded at the depository. The waste produces chemicals and organic matters. Those toxicants contaminate air and water. The pollution causes the increase of insects thereby to spray pesticide on the depository as well as its outskirts. Chemicals and pesticide ingredients are accumulated on the depository and released into outskirts. Those toxic agents are assumed to generate DNA damage to animals exposed to the water and air pollutions. To prove the possibility of DNA damage to pollution, comet assay was conducted on lymphocytes of animals exposed to the pollution of a depository at Southern part of Seoul. Peripheral lymphocytes of animals were treated with endonuclease III and electrophoresed. Broken DNA was released and measured under fluorescence microscope. The measurement showed no statistically significant DNA damage but some individuals showed higher DNA damage than in that of control group. These experiments were carried out on rabbits and dogs, the most and the least contaminated, respectively. The rate of DNA damage of cows was in between that of rabbits and dogs.

Mice에서 Corticoids 투여에 의한 면역성 저하에 대한 Conjugated linoleic acid의 효과에 관한 연구

임형진,김진영,임좌진,이병한,박희명,정병현 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.1

This study was performed to investigate the immunopotentiative effects of conjugated linoleic acid on mice immunosuppressed by administrating corticoids. Mice were divided into four groups of 8 mice. Two groups (C, CP) were given diet supplemented with 1% conjugated linoleic acid (CLA) and the other two goups (L, LP) were given diet supplemented with 1% linoleic acid (LA) instead of CLA. Prednison was administered to two groups (CP, LP) for immune depression. After feeding diets for 3 weeks containing PDS injection for last 1 week. Serum and gut lumen lavage were taken. Measurement of total Ig were executed using sandwich ELISA. Serum levels of IgA, IgG, and IgM showed some trend which groups fed with CLA were higher than groups fed with LA while IgE was reduced in those fed the CLA intake, and groups administered with PDS were lower than groups administered with saline. However, no significant differrences were seen in the proportion of total immunoglobulin in serum. In case of secretory IgA, Group C and CP were significantly higher than group L and LP. Especially between CP and LP, it can be seen effects of CLA. In addition that the CLA treated group weighted a significantly lower level than the one's that have not been treated with CLA. These result support the view that CLA potentiate the immune response and prevent immune depression caused by administrating of corticoids. In conclusion, CLA produced a situation favorable for immunopotentiative effects. Thus, the clinical application of CLA is warranted.

Active biotelemetry를 이용한 젖소의 스트레스 반응 측정: II. 수송 스트레스 감소를 위한 수액투여 효과

이수한,이병한,임좌진,김진영,이동희,김재경,최농훈,정순욱,정병현,Lee, Su-han,Lee, Byeong-han,Lim, Joa-jin,Kim, Jin-young,Lee, Dong-hee,Kim, Jae-kyung,Choe, Nong-hoon,Jeong, Soon-wuk,Chung, Byung-hyun 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

A transport stress is one of the main causes of economic losses and physiological dysfunction. The present study has been performed to suggest a method to decrease the adverse effects above mentioned from transport. The groups were prepared as follows; (1) Control group : 4 cattle transported for 5 hrs (274 km) without any treatment, (2) Treatment group : 4 cattle treated with electrolyte-mineral solution (I.V.) at 1 hr before the enrollment of transport under same experimental condition with Control group. The blood specimens were collected at 1 hr before transport, 2.3 hrs (135 km) and 5 hrs (274 km) after the enrollment of transport, and 1, 6 and 18 hrs after fulfillment of transport. The collected blood specimens were analyzed for cortisol and epinephrine. Core temperature and heart rate were measured with active biotelemetry in every 30 minutes from 0.5 hr before the start of transport to 18 hrs after the end of transport. In results, the level of cortisol considerably increased to the peak either in Control group ($5.3{\pm}1.3{\mu}g/d{\ell}$) and in Treatment group ($4.0{\pm}2.6{\mu}g/d{\ell}$) at 2.3 hrs in transport. The concentration of epinephrine of Treatment group had been higher than that of Control group from the start of transport to 18 hrs after the fulfillment of transport. Particularly there was the biggest gap between Control and Treatment groups, $424.0{\pm}194.1pg/m{\ell}$ and $209.1{\pm}65.1pg/m{\ell}$ respectively, at 6 hrs after the end of transport. The heart rates were considerably increased either in Control group ($81.5{\pm}18.5$ to $126.3{\pm}7.8beats/min$) and in Treatment group ($114.3{\pm}14.4$ to $140.8{\pm}22.4beats/min$) with the enrollment of transport. These results indicate that the concentration of cortisol and the heart rate were pertinent to cognitive parameters to evaluate physiological responses against stress such as transport. In addition, the intravenous administration of electrolyte-mineral solution could be suggested as the method to decrease the adverse effects from a transport stress.

Mouse에서 CLA의 투여가 면역성 증진에 미치는 영향

박종국,김진영,이병한,임좌진,정병현 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.1

Dietary conjugated linoleic acid(CLA) has been shown to affect immune function. Thus, the objective of this study was to investigate the effects of CLA on the mice that treated prednisone. Mice were randomized into 6 groups and fed diet containing either 0(control, P), 0.5%(CLA1, CP1) or 1.5%(CLA2, CP2) CLA for 5weeks. Before 1 week of finishing diet supplement CP1, CP2, and P group treated the prednisone by subcutaneous injection. The levels of serum immunoglobulin A, G, E, gut lumen s-IgA, MLN immunoglobulin A, body weight, mucosal protein was compared. The level of serum IgA in CLA1, CLA2, CP1, and CP2 group increased, while which of P group was decreased. The level of serum IgG in CLA1 group increased, while which of the other group no differences. Serum IgE level showed no difference and the immunoglobulin production in MLN lymphocyte in CLA1 group increased. The level of gut lumen s-IgA in P group showed decreased, while which of the other group showed no differences. These results support the view that CLA supplement partially enhance the cell-mediated immunity and overcome the immunosuppressive effect of prednisone.

Mice에서 CLA의 생물학적 활성이성체의 투여가 면역반응과 체구성에 미치는 영향

최미현,김진영,이병한,임좌진,정재홍,정병현 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.1

Numerous physiological effects are attributed to conjugated linoleic acid(CLA). The purpose of this study is to consider these effects with respect to the cis-9, trans-11 and trans-10, cis-12 CLA isomer. Both isomers are natural products. The c9,t11-CLA isomer is the principal dietary form of CLA, but the concentrations of this isomer and the t10,c12-CLA isomer in dairy products or beef vary depending on the diet fed to cows or steers, respectively. The influence of dietary CLA isomers on the immune response was examined, body weight and weight ratio of organ to body of Balb/C mice. Mice were divided into four groups of 8 mice. Balb/C mice were fed the experimental diets supplemented with 1% CLA (c9,t11-CLA isomer : t10,c12-CLA isomer=2:3) (Group 1), 1% CLA (c9,t11-CLA isomer : t10,c12-CLA isomer=1:1) (Group 2), 1% safflower oil (Group 3) or nothing (Control) for 3 weeks. After 3 weeks, serum, gut lumen lavage, fat, liver, spleen and thymus were taken. Measurement of total immunoglobulin were executed using sandwich ELISA. Serum levels of IgA and IgM showed that group fed with t10,c12-CLA isomer significantly were higher than group fed with c9,t11-CLA isomer. In addition serum level of IgG showed that group fed with t10,c9-CLA isomer significantly were lower than group fed with c9,t11-CLA isomer. However, no significantly differences were observed in the serum IgE and secretory IgA. Weight ratio of spleen to body showed no significant differences. In weight ratio of liver and thymus to body, t10,c9-CLA isomer significantly were respectively higher than group fed with c9,t11-CLA isomer. In weight ratio of fat to body, t10,c9-CLA isomer significantly were respectively lower than group fed with c9,t11-CLA isomer. In conclusion, t10,c12-CLA isomer produced a situation favorable for immunopotentiative effect and body composition. But it should be protected against hepatomegaly induced lipid accumulation in liver.

Prednisolone의 투여에 의한 마우스 비장의 Lymphocyte Subset의 변화

이경리,이병한,김진영,임좌진,정병헌 한국임상수의학회 1999 한국임상수의학회지 Vol.16 No.2

Corticosteroids have long been used for anti-inflammatory, anti-rheumatoid and other purposes in hospital. These effects may be due to inhibit immune reaction. So the animal given corticosteroids was more susceptible to infection because of immunosuppressive effect of corticosteroids. The purpose of this study was to investigate the effects of prednisolone on the lymphocyte subset in the spleen, immunoglobulin in serum, spleen weight, thymus weight and total WBC in peripheral blood. Mice were randomized into 3 groups. Each group has 24 mice. The small dosage group were given by 4 mg/kg/day of prednisolone for 4 days and the large dosage group were given by 8 mg/kg/day respectively. Prednisolone was suspended in saline and orally administered. Mice in control group were given saline alone. Eight mice in each group were sacrificed every week after administration of predisolone. The weight of thymus and spleen were mesured immediately. Lymphocytes were taken from spleen and these cells were analysed by flow cytometry. Also the concentration of total immunoglobulins in serum were assayed by enzyme-linked immunosorbant assay (ELISA). T cell, T-helper cell and T-cytotoxic cell were all significantly (P<0.05) decreased at 1 week after administration of predisolone and at 2 weeks they recovered similarly to that of control. Population of B cell showed various distribution. The concentration of total immunoglobulins in serum was not changed significantly. The weight ratio of spleen to body decreased significantly (P<0.05) during predisolone administration but increased at 1 week later, Eventually the weight ratio was recovered to that of control at 2 weeks. The weight ratio of thymus to body decreased significantly (P<0.05) by prednisolone and recovered gradually up to normal ratio 2 weeks later.