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보육교사의 아동학대 방지를 위한 보육기관 리뷰 및 실시간 CCTV열람 서비스 제공 웹 플랫폼
임종묵(JongMuk Lim),문현호(HyunHo Mun),설경원(GyungWon Seol),신정훈(JungHoon Shin),유철중(Cheol-Jung Yoo) 한국정보기술학회 2021 Proceedings of KIIT Conference Vol.2021 No.6
현대 사회에서 아이를 보육기관에 위탁하는 것은 보호자의 입장에서 필수 불가결하지만 보육교사의 아동학대 문제로 인한 보호자의 불안감은 나날이 증가하고 있다 이로 인해 아동학대 문제를 해결하기 위한 대책 마련의 목소리가 사회적으로 높아졌고 본 논문에서는 보육기관에 대한 리뷰 서비스와 실시간 및 녹화 CCTV 열람 서비스를 제공하는 웹 애플리케이션 시스템 구축을 통해 보육기관의 아동학대 문제를 해결할 수 있는 방안을 제시하고자 한다. In modern society, entrusting children to child-care institutions is indispensable for guardians, but their anxiety over child abuse is increasing day by day. As a result, people have been argued socially to solve the child abuse problem, and in this paper, we propose ways to solve the child abuse problem of child-care institutions through the development of a web application system that provides real-time and recorded CCTV watching services.
임종묵 ( Lim Jong-mook ),권일범 ( Kwon Il-bum ),김치엽 ( Kim Chi-yeop ),최만용 ( Choi Man-yong ),김인걸 ( Kim In-gul ) 한국구조물진단유지관리공학회 1999 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.3 No.1
The strain calibration of PVDF(polyvinylidene fluoride) sensor was performed by Al beam test. The PVDF sensor and the electrical strain gage were bonded on the beam. When the beam was loaded, the output of electrical strain gage was compared with the output of the PVDF sensor. In the case of that the charge amplifier was no used in the experiment, the waveform of PVDF sensor output was shown as the differential waveform of the output of the electrical strain gage. In another case, that was applied by the charge amplifier, the waveform of PVDF sensor output was shown as the same form of the output of electrical strain gage. The maximum amplitude of PVDF sensor output was only slightly different with the maximum amplitude of electrical strain gage output in the case of using the charge amplifier. Therefore, the gain was selected as 1.7 by comparing these two signals to determine the exact value of the strain.
임종은,Irina Krier,임정묵,심수진,한동근,Jeffrey A. Hubbell 한국고분자학회 2015 Macromolecular Research Vol.23 No.4
Polyethylenimine (PEI) is a promising gene carrier among polymeric vectors because of its high transfectionefficiency, buffering capacity, which is responsible for its escape from endosomes, facility of modification,reasonable price and wide range of molecular weight. However, toxicity of PEI is an important obstacle to overcomefor using it as a gene carrier in gene therapy protocols. In our study, PEI (10 kDa) was modified with urocanic acid,which has an imidazole ring (PEIU). This modification of PEI played an important role by improving transfectionefficiency and reducing toxicity. The PEIU was condensed with DNA in different nitrogen of polycation/phosphateof DNA (N/P) ratios starting from 1.25. The PEIU/DNA complexes formed smaller (60-80 versus 100-400 nm) andless dispersible particles than PEI/DNA complexes. Cell viability test showed that in all the transfection experimentsPEIU was always found less toxic than the PEI on three different cell-lines, Cos-7, HeLa, and 293T. The PEIUdemonstrated improved biocompatibility as compared to the PEI. The in vitro transfection experiments showed thatthe PEIU displayed similar or lower transfection efficiencies than the PEI in the absence of serum. In medium complementedwith serum, the PEIU had higher transfection efficiencies than the PEI. In vivo transfection experimentswere carried out with intravenous and subcutaneous injections into mice. Five different organs were taken and lysedfor quantification of expressed GFP. The quantification of the fluorescence of these organs exhibited that the PEIUhas better transfection efficiency in in vivo experiments compared to the PEI. This study generated a strong evidenceindicating that PEIU can be considered as a promising versatile gene carrier because of its biocompatibility and goodtransfection efficiency.
임종은,Jeffrey A. Hubbell,이승태,한동근,임정묵 한국고분자학회 2015 Macromolecular Research Vol.23 No.4
The development of an in vitro culture system, comparable to the in vivo environment in terms of effectson oocyte growth and development, could provide a valuable experimental tool for studying the mechanisms governingoocyte development as well as practical clinical, agricultural, zoological, and biotechnological applications. This studyreports on the importance of the microenvironment for the ovarian folliculogenesis process. The complexity of sucha microenvironment was approached with a strategy based on functionalized PEG-hydrogels. The PEG matrix notonly serves as a scaffold, but it is also used a reservoir of immobilized cues. Using tethered integrin-binding peptidesin combination with other signaling factors, we aimed at better understanding the interactions of the oocyte and itssurrounding granulosa that may determine the efficiency of the actual and the future in vitro mature oocyte production. Ina first step, the mechanical properties of PEG-hydrogel were optimized for producing secondary follicles, in whichthe oocyte is surrounded by two layers of granulosa cells. Follicle growth was highly dependent on the mechanicalproperties of the surrounding environment, with the optimal elastic modulus being approx. 1 kPa. The effects of keysoluble factors were also investigated to confirm their compatibility with the established 3-D culture system and tofurther qualitatively and quantitatively improve the produced mature oocytes. Accordingly, various combinations ofthe gonadotropins follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were tested. Interestingly, theeffects of the gonadotropins in the 3-D PEG system were close to their known in vivo effects. In conclusion, this studydemonstrates the efficiency and the flexibility of a novel 3-D culture system, PEG-hydrogel. Circumventing problemsinherent to the “on-plastic” standard culture, such as the loss of the granulosa-oocyte interactions, allowed the emergenceof a culture system tailored for investigating fundamental folliculogenesis-related questions. Furthermore, the reportedculture system may serve as a platform for developing clinical and biotechnology applications.