FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향

이호균(Ho Kyun Lee),정상영(Sang Young Chung),최수진나(Soo Jin Na Choi) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.5

Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells. Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry. Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1. Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.

커피전문점 선택속성의 만족도에 기초한 점포 입지 및 고객특성별 세분화모형에 관한 연구

이호병(Lee Ho byung) 한국부동산학회 2014 不動産學報 Vol.59 No.-

본 논문은 커피전문점 선택속성 만족도를 토대로 점포의 입지 및 고객특성에 의한 세분화 모형을 분석하고자 한다. 본 연구의 주요 결과는 다음과 같다. 첫째, 차우검정 결과, 성남시 중북부지역에서 커피전문점 상권을 분석하는 경우 입지 및 고객특성에 의한 세분화 모형이 필요하다. 둘째, 요인분석의 결과, 6개 요인(커피/서비스, 점포시설, 부가성, 입지, 영업, 승용차 이용편리성)이 커피전문점 만족도와 관련하여 통계적으로 유의미한 요인으로 작용하고 있음을 알 수 있었다. 1. CONTENTS (1) RESEARCH OBJECTIVES The purpose of this research is to analyze the segmentated model by coffee shop's locations and its customer characteristics based on the satisfaction of its selection attribute. The data of the research were collected from the customers of the coffee shops in the northern-central region of Sungnam-si. (2) RESEARCH METHOD This study was focused on survey analysis. The data for the analysis were collected from the questionnaire. This research used the statistical analysis techniques such as basic statistics analysis, factor analysis, regression analysis and Chow-test. (3) RESEARCH FINDINGS This research showed that the results of Chow-test rejected the null hypothesis of identity for regression coefficients with regard to store location, gender, age, education, income and franchise/general coffee shop preference at the 5% significance level. 2. RESULTS The result of this research produced the following two findings. First, the segmentated model by location and customer characteristics, as a result of Chow-test, are needed in case of investigating the trade area of coffee shop in the northern-central region of sungnam city. Second, the results of factor analysis showed that the 6 factors such as coffee/service, store facility, additional characteristics, location, store management and car traffic convenience were statistically significant in regard to the satisfaction of coffee shop.

소규모 마을하수도 시설의 폐수특성 및 처리공정의 시뮬레이션 특성

이호식 ( Ho Sik Lee ),임란 ( Ran Im ),조경식 ( Kyeong Sik Cho ) 한국수처리학회 2009 한국수처리학회지 Vol.17 No.3

한국 석비石碑 양식의 변천

이호관(Lee ho-kwan) 한국국학진흥원 2002 국학연구 Vol.1 No.-

B 형 만성활동성간염 치료제로서 합성 α - Interferon 의 효용성

이호심(Ho Sim Lee),임종찬(Jong Chan Lim),이일남(Il Nam Lee),조현덕(Hyun Duk Cho),유병철(Byung Chul Ryoo),박실무(Sill Moo Park) 대한내과학회 1990 대한내과학회지 Vol.39 No.3

N/A Fifty eight patiens with chronic active hepatitis were entered into a therapeutic trial of 12-week period of recombinant α-interferon therapy. All patients were positive for both HBsAg and HBeAg. During the 13 to 62-week period of follow-up, 24 of 58 (47%) treated patints became negative for serum HBeAg but in 9 of these HBeAg reappeared in their serum. Fifteen patients (30%) were also positive for serum Anti-HBe but 5 of this lost Anti-HBe from serum. 9 patents positive for initial HBV DNA lost their DNA from serum. Responders had a marked improvement in serm aminotransferase activities. Seroconversion rate of female (5/9, 56%) was seemed to be higher than that of male (19/43, 45%). Comparison of responders to non-responders indicated that a high initial level of serum aminotransferuse and a severe form of hepatic histology correlate best with response to interferon herapy. 45 of 58 (78%) patients showed various adverse reactions, such as myalgia (59%), fatigue (57%), fever (52%), nausea and anorexia. Transient decrease in peripheral leukocyte and platelet count were also noted. These findings suggest that α 12-week course of recombinant α-interferon therapy would be effective and safe in approximately half of patients with chronic active hepatitis B.

한국형 고무차륜경량전철시스템(K-AGT) 신뢰성평가 연구

이호용(Ho-Yong Lee),이안호(An-Ho Lee),조홍식(Hong-Shik Cho),홍재성(Jai-Sung Hong),한석윤(Seok-Youn Han) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.11

Korea Railroad Research Institute(KRRI) developed the Driverless Rubber Tired Korea-AGT (Model: K-AGT) from 1999 to 2004. We have finished the safety and performance tests of K-AGT, but the reliability test result was not effective level. In the paper, analyzed whether can improve in this research through authoritativeness growth analysis to get satisfied result. Data obtained from this testing can be used to evaluate the growth of reliability. The most widely used traditional growth tracking model5 is included as IEC International standard. In this paper, we demonstrated reliability analysis using growth model of driverless rubber tired K-AGT system to prove reliability of development system. Therefore, we introduce the well-known NHPP model and analyze a reliability growth using ReliaSoft's RGA software.

부동산경매정보서비스에 대한 고객의 재이용 영향 구조 분석 -정보컨텐츠를 중심으로 -

이호병 ( Lee Ho Byung ) 한국부동산학회 2012 不動産學報 Vol.50 No.-

1. CONTENTS (1) RESEARCH OBJECTIVES The purpose of this study is to analyze a structure of the customer's reusing affect for real estate auction information service in the respect of its the information contents using statistical analysis techniques. (2) RESEARCH METHOD This study was focused on survey analysis. The data for the analysis were collected from the questionnaire. This study used the statistical analysis techniques such as factor analysis and structural equation modeling. (3) RESEARCH FINDINGS In the result of this study, the profitability-related factor was the greatest influence on the satisfaction for the information contents of real estate auction information service among the three factors such as object content service, after-bidding service and profitability-related service. 2. RESULTS Each of the three factors did not showed the significant influence on the reuse of real estate auction information service. However, the three factors showed the significant influence on the satisfaction of the information contents of real estate auction information service. Furthermore, their satisfaction affected the reuse significantly.

투명 세포 극세포종

이호균(Ho Gyun Lee),조광현(Kwang Hyun Cho),이유신(Yoo Shin Lee),함의근(Eui Keun Ham),송계용(Kye Young Song),김원석(Won Suk Kim) 대한피부과학회 1990 대한피부과학회지 Vol.28 No.5

We report a case of clear cell acanthoma occurred in a 61 year old man. He developed a brownish bean sized nodule on the left chest 3 years ago. The skin lesion has slowly increased in size without any subjective symptoms. Histopathologic examination revealed proliferation of clear cells within a sharply demarcated area of the epidermis. The presence of numerous neutrophils throughout the epidermis were also noted. (Kor J Dermatol 28(5): 642-645, 1990)

CORBA 를 이용한 객체 지향 분산 멀티미디어 응용 시스템 구현

이호동(Lee Ho Dong),김영곤(Kim Young Gon),배두환(Bae Doo Hwan),전예임(Jeun Yame),송태섭(Song Tae Seop) 한국정보과학회 1997 한국정보과학회 학술발표논문집 Vol.24 No.2Ⅰ

분산 환경이 가속화 됨에 따라 분산 시스템을 위한 응용 소프트웨어에 대한 요구가 발생하였고, 분산 멀티미디어 응용 개발에 대한 필요성도 점점 증가하고 있다. 본 논문은 분산 멀티미디어 응용 소프트웨어 구현 시 발생되는 어려움을 줄이기 위해 상호 운용성, 객체 지향 개념, 투명성 등을 제공하는 CORBA를 사용하여 분산 멀티미디어 응용에 적용 하였다. CORBA는 멀티미디어 특성이 객체 지향 개념에 잘 부합되고, 분산 환경에 대한 지원이 제공되기 때문에 CORBA를 사용하여 분산 멀티미디어의 개발은 매우 효율적이다. 분산 시스템에서의 응용 소프트웨어를 쉽게 개발할 수 있도록 도와주는 미들웨어인 CORBA 환경하에 멀티미디어 정보를 검색하는 시스템을 구현하였다.

야관문(夜關門)의 항염증효과(抗炎症效果)

이호일 ( Ho Il Lee ),정지윤 ( Ji Yun Jung ),황보민 ( Min Hwang Ho ),구세광 ( Sea Kwang Ku ),김영우 ( Young Woo Kim ),지선영 ( Seon Young Jee ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.4

Objectives : Lespedeza Cuneata has been used to treat leukorrhea, asthma, stomach pain, diarrhea, acute mastitis, in Korean traditional medicine. According to recent studies, Lespedeza Cuneata has antioxidation, hypoglycemia, cell protective, insulin secretion, whitening, corpora cavernosa smooth muscle relaxation and antimicrobial activities, but it has been rarely conducted to evaluate the immuno-biological activity. The present study was examined to evaluate the anti-inflammatory effects of the Lespedeza Cuneata MeOH extract (LCE) in vivo and in vitro. Methods : In vitro, inflammatory mediators, such as cytokines, nitric oxide and prostaglandin E2 were detected after the addition of LPS with or without LCE in Raw 264.7 macrophage cell line. In vivo, anti-edema effect of LCE was determined in the carrageenan-induced paw edema model in rats. Results : In vitro assay, LCE decreased release of nitric oxide (NO) and prostaglandin E2 (PGE2) via suppression of iNOS and COX-2 expression. LCE inhibited the phosphorylation of IĸB indicating the suppression of NF-ĸB pathway. In vivo assay, LCE significantly inhibited the formation of paw edema induced by carrageenan injection in rats. LCE effectively inhibited increases of hind paw skin thickness and inflammatory cell infilterations. Conclusion : These findings demonstrate that LCE has inhibitory effect on inflammatory mediators in LPS-activated Raw 264.7 cells and on paw edema in carrageenan-stimulated rats, showing the possibility of anti-inflammatory use of Lespedeza Cuneata.