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정영희,강지연,임은희,이은주,여수정,진전성,이경아,김영호,강종성 충남대학교 약학대학 의약품개발연구소 2005 藥學論文集 Vol.20 No.-
Herbal drugs have been playing an important role in the medical therapy system in the oriental culture for thousands of years. Nowadays, the needs for the quality evaluations of these materials are very strong. In this research costunolide in Saussureae Radix was analyzed and the content of regulation was established for the quality evaluation of Saussureae Radix. Costunolide in the acetone extract of Saussureae Radix was successfully separated on Zorbax Eclipse XDB C_(18)(2.1×150 ㎜) with methanol-water(65:35, v/v) as mobile phase. The flow rate was 0.25 ㎖/min and detection was carried out at UV 215 ㎚. The HPLC method was validated by ICH guide lines comparing the linearity, precision, accuracy and detection limit. The average content of costunolide in Saussureae Radix was 1.04%. The highest costunolide content among the samples was 18 times higher than the lowest content. The content of costunolide for regulation in Saussureae Radix, set as the lowest costunolide content of analyzed sample which lied in the range of 95% confidence interval, was 0.68%. The appropriate number of samples to establish the costunolide content for regulation was proposed as 20, with the error level of not higher than 10%. These results provided the statistical back ground for the quality evaluation of Saussureae Radix.
당뇨병 Goto-Kakizaki 랫트에서 호모시스테인의 대사조절
오정민,여수정,김봉희,김상겸 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10
Elevation of homocysteine levels is a risk factor for cardiovascular diseases and liver diseases. It has been reported that both streptozotocin-induced type I diabetic rats and obese type II diabetic rats have plasma total homocysteine lower than each control rats. We determined the effects of lean type II diabetes on homocysteine levels using type 2 diabetic Goto-Kakizaki rats. The concentrations of serum glucose were increased to ∼two-fold of control levels and the total cholesterol levels were also increased in GK rats. Hepatic aspartate, histidine, threonine, alanine and methionine levels were significantly increased in GK rats. Plasma aspartate and glutamate levels were elevated, but threonine and arginine levels were decreased in GK rats. Plasma total homocysteine levels were not changed in GK rats, but hepatic total homocysteine levels were increased to ∼-three-fold of control levels. These results suggest that hepatic metabolism of sulfur-amino acid may be altered in diabetic condition.