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대한간학회지 제6차 춘계학술대회 초록집 : 구연 ; Differential Gene Expression을 이용한 간세포암 형성에 관여하는 유전자 규명
송정엽 ( Song Jeong Yeob ),최정희 ( Choe Jeong Hui ),김유진 ( Kim Yu Jin ),이광재 ( Lee Gwang Jae ),유병무 ( Yu Byeong Mu ),함기백 ( Ham Gi Baeg ),김진홍 ( Kim Jin Hong ),조성원 ( Jo Seong Won ) 대한간학회 2000 Clinical and Molecular Hepatology(대한간학회지) Vol.6 No.1(S)
재발성 구강내 진균감염증이 동반된 IgG3 아형 결핍증 1례
최정희,박해심,송정엽,이수걸,남동호,유상용,김선신 대한알레르기학회 2000 천식 및 알레르기 Vol.20 No.4
Common clinical manifestations in patients with IgG subclass deficiency include recurrent respiratory tract infection, recurrent otitis media and sinopulmonary infection by virus or bacteria. The administration of intravenous immunoglobulin(IVIG) has been regarded as the most effective therapy in these patients. We experienced a 22-year-old patient with IgG3 subclass deficiency and recurrent fungal infection of oral cavity and lips. IVIG was given at 0.2g/kg/dose twice a month for 6 months. After treatment with IVIG, the patient improved clinically.
유방암 환자의 말초혈액에서 역전사효소연쇄중합반응을 이용한 Human Mammaglobin 측정의 임상적 유용성
김재홍,강석윤,송정엽,최태영,임홍석,김선경,김영진,박준성,김현수,최진혁,임호영,김효철 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.2
Background: The mammaglobin gene encodes a novel protein that is secreted from the mammalian epithelium of normal breast tissue as well as malignant breast cancer tissues. In order to ascertain the prognostic value of mammaglobin gene in breast cancer patients, we measured the expression of human mammaglobin (hMAM) by RT-PCR method in various stages of breast cancer patients. Methods: Peripheral blood samples from forty healthy volunteers and 114 breast cancer patients were obtained. Peripheral blood stem cells (PBSC) collected for the purpose of autologous stem cell transplantation in five patients with metastatic breast cancer and ten patient with high risk for relapse and no evidence of disease were used for hMAM assay. Results: All samples from peripheral blood of forty healthy individuals (twenty males and twenty females) were negative for hMAM, whereas 43 of 114 samples (38%) from breast cancer patients were positive for hMAM mRNA. All the normal breast tissues were positive for hMAM mRNA. hMAM mRNA expression was detected in 11 of 42 (26%) in breast cancer patients who underwent for curative resection and had no evidence of disease, in 8 of 25 (34%) with chemo-sensitive relapsed disease, and in 16 of 32 (53%) with chemo-refractory progressive disease. Eight (53%) samples from peripheral blood of 15 breast cancer patients with metastatic disease at diagnosis were positive for hMAM. Three (20%) samples from peripheral blood stem cells of 15 breast cancer patients for high dose chemotherapy were positive for hMAM. Conclusion : In contrast to healthy volunteers, hMAM transcripts were detected in the peripheral blood of breast cancer patients. The frequency of hMAM expression in peripheral blood was correlated with the clinical stages of disease, but, was not significant. The contamination of hMAM expressing cells in the stem cell pool warrants additional effective purging method before the transplantation. The clinical relevance of hMAM RT-PCR-based tumor cell detection in the peripheral blood of breast cancer patients should be further evaluated in prospective studies.
임영애,전희선,곽연식,송정엽 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1
Introduction: To evaluate the usefulness of Widal test requested only once during the clinical episode(single Widal test) in diagnosing typhoid fever, results of culture for Salmonella typhi were compared to the results of single Widal test. Materials and Methods: Widal tests were performed by rapid slide melhod(stained Salmonella suspensions, Murex, England) and the cutoff values of Widal test liter were ≤ 1:160 for 0 antigen, ≤ 1:320 for H antigen. After reviewing culture results of S.typhi the sensitivity, specificity and efficiency of the single Widal test were computed. Results: Culture results of S.typhi in positive Widal test were 7.1%(9/126) for 0 antigen, 7.6% (11/134) for H antigen, 10.9%(6/55) for 0 & H, therefore false positive results were 92.9% for 0, 92.4% for H, 89.1% for 0 & H combined and false negative results were 0.7%(7/1022) for 0, 0.5% (5/1003) for H, 0.2%(2/1093) for 0 & H. The sensitivity, specificity and efficiency of single Widal test were 56.3% (9/16), 89.7%(1015/1132) and 89.3%(1026/1148) for 0; 68.8%(l 1/16), 88.2% (998/1132) and 87.8%(1009/1148) for H; 37.5%(6/16), 95.7%(1083/1132) and 94.9%(1089/1148) for O & H, respectively. Also, Widal test results were positive on many occasions when group D Salmonella was isolated even if isolates were not S.typhi and some group B Salmonella isolates, Conclusion: The outcome of single Widal test showed more than a 90% false positive rate for both 0 and H antigens. Furthermore, Widal tests were positive when patients were infected with Salmonella groups other than S.typhi. Therefore, it is concluded that single Widal test is not a good diagnostic test for typhoid fever.
간세포암종과 관련된 유전자 규명을 위한 Differential Gene Expression 의 이용
조성원,이광재,김진홍,최정희,함기백,송정엽,유병무 대한간학회 2001 Clinical and Molecular Hepatology(대한간학회지) Vol.7 No.3
Background/Aims It has been acknowleged that diverse factors such as Hepatitis B or C virus, alcohol, food carcinogens, and environmental or genetic factors are involved in hepatocellular carcinogenesis. In the molecular biologic aspect, suppression of tumor suppressor gene or amplification of oncogene, abnormal regulation of cell cycle-related proteins, abnormal apoptosis mechanism, and diverse growth factors are reported to be factors that contribute to hepatocellular carcinogenesis. In this study, the genetic difference between hepatocellular carcinoma tissue and surrounding non-hepatocellular carcinoma tissue has been investigated to identify genes that are deleted, diminished, amplified, or newly developed in hepatocellular carcinoma using differential gene expression. Method: We studied each of 12 biopsy samples of hepatocellular carcinoma and surrounding non-hepatocellular carcinoma tissues obtained during surgical resections. Random arbitrarily primed-polymerase chain reaction (RAP-PCR) was applied for differential gene expression. The genes that are deleted, diminished, or amplified, newly developed in hepatocellular carcinoma are cloned, sequenced, and then identified by BLAST search, some genes are characterized by eletrophoresis motility shift assay (EMSA) and in situ hybridization. Results: We identified the various, diverse genes classified as tumor suppressor genes, oncogenes, growth factor genes, and some kinds of transcription factors. Some of these genes were identified to be repressed, deleted or diminished, others were amplified, or newly developed in hepatocellular carcinoma tissues. Conclusions: RAP-PCR is a good method in the identification of the gene associated with hepatocellular carcinoma. The result in this study shows that so many genes are different between hepatocellular carcinoma and surrounding non- hepatocellular carcinoma tissues, and that the genes related with hepatocellular carcinogenesis may be predicted. Further studies are necessary for analyzing the relationship between the identification of the gene associated with hepatocellular carcinoma and the diverse factors involved in hepatocellular carcinogenesis. (Korean J Hepatol 2001;7:265-272)