황련해독탕약침(黃連解毒湯藥針)을 이용한 흉부상열감(胸部上熱感) 치험(治驗) 2례(例)

김종욱,이윤재,임은경,신선호,김동웅,홍석훈,주종천,조권일,Kim, Jong-Uk,Lee, Yun-Jae,Rhim, Eun-Kyung,Shin, Sun-Ho,Kim, Dong-Woung,Hong, Seok-Hoon,Joo, Jong-Cheon,Cho, Gwon-Il 대한약침학회 2003 Journal of pharmacopuncture Vol.6 No.2

The purpose of this study is to evaluate the clinical effect of Hwangryunhaedok-tang Herbal-Acupuncture. Hwangryunhaedok-tang is used in all heating diseases. Chest Heating Sensation is a unique concept in Oriental Medicine. So we applied Hwangryunhaedok-tang Herbal-Acupuncture to treat the Chest Heating Sensation. We used DITI(Digital Infrared Thermographic Imaging) to estimate the temperatures of chest surface for the outcome assessment. We came to know that the chest surface temperatures were all reduced in both cases after Herbal-Acupuncture treatment. The reduced average temperature was $1.5^{\circ}C$ in case 1 and $0.9^{\circ}C$ in case 2. The above result indicates that Hwungryunhaedok-tang Herbal-Acupuncture treatment has an effect on Chest Heating Sensation, thus continuous Hwangryunhaedok-tang Herbal-Acupuncture study will be needed for more clinical applications.

하천의 어제, 오늘 그리고 내일 - 공지천 유역의 생태하천복원사업

김종욱,Kim, Jong-Uk 한국하천협회 2010 하천과 문화 Vol.6 No.2

가와사키병에 의한 관상동맥류의 관상동맥우회로술 -치험 1례-

김종욱,이재원,송명근,Kim, Jong-Uk,Lee, Jae-Won,Song, Myeong-Geun 대한흉부심장혈관외과학회 1995 Journal of Chest Surgery (J Chest Surg) Vol.28 No.4

Kawasaki s disease is a multisystemic disorder that is an important cause of cardiovascular disease in children. We experienced a case of coronary artery aneurysm secondary to Kawasaki s disease. The patient was 12-year-old female presented as exertional dyspnea & chest pain[NYHA classII . Her coronary angiogram showed saccular aneurysms at proximal right coronary artery and proximal left anterior descending artery. CABG was performed by use of double internal mammary artery.Postoperative E.K.G. showed a normal pattern.She discharged at postoperative 10th day without problem. 4 months after discharge, she had no dyspnea & chest pain in exertion[NYHA class I .

척추관내 인대 골화증 6례에 대한 임상적 고찰 -후종인대 골화증(OPLL) 3례 및 황색인대 골화증(OLF) 3례-

김종욱,최성용,황우준,이순호,유인식,Kim, Jong-uk,Choi, Sung-yong,Hwang, Woo-jun,Lee, Sun-ho,Yoo, In-sik 대한침구의학회 2004 대한침구의학회지 Vol.21 No.6

Objective : Ossification of ligaments within spinal canal, i.e., OPLL and OLF, is uncommon clinical entity as a cause of the progressive compression myelopathy or radiculopathy. More and more cases being reported in the field of occidental medicine, but very few cases have been reported in the field of oriental medicine. The purpose of this study is to report on oriental medical approaches to OPLL and OLF. Methods : Subjects of this study are 3 cases of OPLL and 3 cases of OLF who visited Won-kwang oriental medical hospital(Dept. of acupuncture and Moxibustion) from May, 2002 to October, 2003. These patients undergo oriental medical treatment such as acupuncture, cupping, Bee-Venom therapy and herbal medication and so on. We made a comparison JOA scores between before treatment and after treatment and we evaluated results of treatment. Results : The results of treatment in these six cases are as follows ; One case was evaluated 'Excellent', one case was evaluated 'Good', two cases were evaluated 'Fair' and two cases were evaluated 'Failure'. One of these cases had a surgical operation after discharge from this hospital. Conclusions : After oriental medical care for these cases, there are some improvements such as decrease of pains, relief of myelopathy etc. But, it had little effect on some cases, therefore we considered that more special study to find various and effective methods of oriental medical treatment for these diseases should be made.

새로운 유전자 재조합 방법을 이용한 대장균에서의 인간 tissue inhibitor of mtrix metalloproteinase-2 (TIMP-2) 유전자의 가용성 발현

김종욱,최동순,주현,민철기,Kim, Jong-Uk,Choi, Dong-Soon,Joo, Hyun,Min, Churl-K. 한국생물공학회 2008 KSBB Journal Vol.23 No.3

암세포의 침윤은 숙주 조직의의 기저막과 세포외 기질을 침투함으로써 일어난다. 침윤과 전이과정에는 단백질가수분해 효소인 matrix metalloproteinases (MMPs)가 깊이 연관되어 있는 것으로 알려져 있으며, MMP의 가수분해 활성은 tissue inhibitors of metalloproteinases (TIMPs)라는 억제 단백질에 의해 억제된다. TIMP-2는 21kDa 크기의 포유류 단백질로 대장균에서 과발현 시 다른 많은 포유류 단백질과 마찬가지로 가용성이 낮은 봉입체 형태로 발현된다. TIMP-2 단백질의 접힘에 6개의 이황화결합이 필요하고, 이는 일반적으로 대장균 환경은 적합하지 않다. 본 연구에서는 대장균에서 불가용성으로 발현되는 TIMP-2 유전자를 유전자셔플링 기법의 한 가지인 StEP (staggered extension process)를 변형하고 동시에 $Mn^{2+}$ 농도 변화와 dGTP 불균형을 이용한 무작위 돌연변이 기법을 혼용하여 대장균에서 가용성 TMP-2 재조합 변이체를 생성하고자 하였다. 무작위로 재조합된 TIMP-2 유전자 중에서 가용성으로 발현되는 TIMP-2 유전자를 선별하기 위해서 chloramphenicol acetyltransferase (CAT)-융합 방법을 도입하였다. CAT 유전자가 가용성으로 발현되는 재조합 TIMP-2 유전자에 융합되면 이를 갖는 E. coli는 높은 chloramphenicol 환경에서 생존이 가능하게 된다. 이러한 in virro mutageuesis 기법과 CAT-융합 방법으로 대장균 가용성 TIMP-2 재조합 변이체를 14가지 얻을 수 있었다. 변이체 TIMP-2의 아미노산서열 분석과 구조 분석 결과 주로 소수성 아미노산이 친수성 아미노산으로 전환되었고, MMP와의 결합이 관여하지 않는 C-말단 부위에 돌연변이가 집중되어 있었다. 본 연구에서 개발된 간편하고 새로운 in vitro 재조합 방법과 CAT을 이용한 스크리닝 기법은 다른 많은 대장균 내 불가용성 단백질의 발현에도 사용될 수 있을 것으로 사료된다. The second family member of tissue inhibitors of matrix metalloproteinases, TIMP-2, is a 21kDa protein which inhibits matrix metalloproteinases 2 (MMP-2). Expression of mammalian proteins in E. coli often forms inclusion bodies that are made up of mis-folded or insoluble protein aggregates. The requirement for the formation of 6 disulfide bonds in the process of the TIMP-2 folding is likely to be incompatible with the reducing environment of E. coli. However, this incompatibility can be often overcome by introducing a mutagenesis that could lead to enhancement of the protein solubility. In this reason, we have attempted to express the soluble TIMP-2 in E. coli by applying a modified staggered extension process (StEP), one of the in vitro PCR-based recombinant mutagenesis methods, and error-prone PCR. C-terminally located CAT fusion protein with respect to mutated TIMP-2 proteins enables us to differentiate the soluble TIMP-2 from the insoluble in E. coli by virtue of chloramphenicol resistance. According to this scheme, E. coli harboring properly-folded CAT fused to TIMP-2 protein was selected, and some of the resulting colonies exhibited an enhanced, soluble expression of TIMP-2 compared to the wild type, implying (i) the StEP technique is successfully employed to enhance the proper folding thereby increasing the solubility of TIMP-2, and (ii) the CAT dependent screening may be a simple and effective method to differentiate the soluble protein expression in E. coli.

시간 스케일러빌리티를 이용한 MPEG-4 FGS 비디오 부호화 기법

김종욱,이배호 전남대학교 전자통신기술연구소 2002 전자통신기술논문지 Vol.5 No.1

The paper shows MPEG-4 FGS(Fine Granularity Scalability) using the scalable video encoding technique that is adapted by MPEG-4 standard and a scheme to improve its efficiency. MPEG-4 FGS is the encoding technique that has the good flexibility for various property of devices and bandwidth variation over the internet. To improve MPEG-4 FGS coding efficiency, we applied an encoding technique with temporal scalability. In other words, the base layer is encoded with the same technique as the typical MPEG-4 FGS and the enhancement layer is encoded by adding temporal scalability property In test results, we could know that had better coding efficiency in the case of adding temporal scalability for the enhancement layer.

온라인 소비자 구매결정과정에서의 제품관여도 효과에 관한 연구

김종욱,박상철,Kim, Jong-Uk,Park, Sang-Cheol 한국경영정보학회 2005 Asia Pacific Journal of Information Systems Vol.15 No.3

This research is to investigate the effects of product involvement on the selection of online shopping malls. From the statistical analysis of 282 response data, it was found that consumers buying products with higher involvement tend to shop in a shopping mall with greater trust, which provide more information regarding their products and a wider variety of products. The other purpose of this research is to investigate factors of shopping malls which influence the buyer's purchase intention. The results of data analysis indicate that consumers are more likely to buy in a shopping mall with greater trust, with detailed product information, and with higher entertaining contents. The results of the study indicate that the shopping malls which give more detailed product information to their customers and establish greater trust will finally lead to a higher sales revenue because more expensive products will be sold.

행간(行間)(LR2) 전침자극(電鍼刺戟)이 적외선(赤外線) 체열진단상(體熱診斷上) 안면부(顔面部) 온도변화(溫度變化)에 미치는 영향(影響)

김종욱,최성용,진경선,황우준,민상준,이순호,이상룡,Kim, Jong-uk,Choi, Sung-yong,Jin, Kyong-son,Hwang, Woo-jun,Min, Sang-jun,Lee, Sun-ho,Lee, Sang-ryong 대한침구의학회 2004 대한침구의학회지 Vol.21 No.1

Objective: Purpose of this study was to examine the effect of electroacupuncture(EA) at Xingjian(LR2) as 'Fire(火)' point of The Leg Absolute Um Liver Meridan(足厥陰肝經 : Chok-Kworum-Kan-Kyong) on the facial thermal change. Methods: Subjects of this study were 15 patients with upperpart(includes head and facial part) fever of human body and two examinations were carried out in each other day. We divided cases of two examinations into two groups. One is experimental group(N=15) that was carried out electroacupuncture stimulation at Xingjian(LR2), the other is control group(N=15) which was carried out electroacupuncture stimulation at optional point(in space between 1st and 2nd fingers) except acupuncture points of 12 meridians. We took the temperature of fixed areas on face by digital infrared thermal image(D.I.T.I.) before and after electroacupuncture stimulation. Those fixed areas on face that was taken temperature are Jingming(BL1), Sibai(ST2), Dicang(ST4), Indang, Shuigou(GV26), Chengjiang(CV24) areas. In cases of temperature of Jingming(BL1), Sibai(ST2), Dicang(ST4) areas, we applied each mean of left and right temperature to statical analysis. Results: In the group of electroacupuncture stimulation at Xingjian(LR2), temperature of every fixed areas on face fell: Jingming(BL1) area's ${\Delta}T=-0.7007{\pm}0.78642$, Sibai(ST2) area's ${\Delta}T=-0.6280{\pm}0.56439$, Dicang(ST4) area's ${\Delta}T=-0.5940{\pm}0.60179$, Indang area's ${\Delta}T=-0.7200{\pm}0.64515$, Shuigou(GV26) area's ${\Delta}T=-0.6160{\pm}0.80487$, Chengjiang(CV24) area's ${\Delta}T=-0.5627{\pm}0.72615$. In Xingjian(LR2) electroacupuncture group, each temperature of Jingming(BL1), Sibai(ST2), Indang areas showed a drop significantly in comparison with control group (p<0.05). But each temperature of Dicang(ST4), Shuigou(GV26), Chengjiang(CV24) areas did not showed a drop significantly in comparison with control group(p>0.05). Conclusions: The results mentioned above showed that electroacupuncture stimulation at Xingjian(LR2) significantly decreased the temperature on face of patients with upperpart fever of human body. In Xingjian(LR2) electroacupuncture group, especially temperature of upper part of face includes eye, cheekbone, forehead regions showed a drop significantly in comparison with control group.

품질에 의한 커피 원두의 분류

김종욱,Kim, Jong-Uk 한국식품연구원 2008 한맛한얼 Vol.1 No.4

커피원두의 품질평가는 커피가 생산되는 나라마다 규격이 다르고 매우 다양한 원두가 생산되고 있어 오랜 기간 동안 숙련을 거친 사람만이 할 수 있는 작업이다. 원두의 품질을 제대로 평가할 능력이 없을 경우 저가의 원두를 고가를 지불하고 구매하는 경우도 발생하며 일정한 품질의 원두를 구매할 수 없어 균일한 품질의 제품을 생산할 수 없게 되므로, 커피 제조업체에서는 원두 품질평가자가 매우 중요한 역할을 담당하게 된다. 커피가 재배되는 산지에서는 커퍼(Cupper)라고 불리는 품질평가자들이 엄청난 양의 품질을 평가하고 가격을 결정하는 일을 담당하고 있다.

백두옹탕(白頭翁湯)의 대장암 세포주 HCT-116 항암효과와 세포자멸사에 관한 연구

김종욱,문구,박찬희,이정한,지혜민,Kim, Jong-Uk,Moon, Goo,Park, Chan-Ny,Lee, Jeong-Han,Ji, Hye-Min 대한한방내과학회 2010 大韓韓方內科學會誌 Vol.31 No.2

Objectives : To investigate the anti-cancer effect of Baekduong-tang(BDOT) against cancer cells, the signaling pathway of apoptosis was explored in human colon cancer cells. Materials and Methods : Human colon cancer cell lines, including HT-29 and HCT-116 cells, were used. Cell viability was measured by MTT assay. Apoptosis was determined by DAPI nuclei staining and flow cytometry in HCT-116 cells treated with 0.25 mg/$m{\ell}$ Baekduong-tang for 48 hrs. Results : Baekduong-tang induced the apoptosis of p53 positive HCT-116 cells with G2/M phase arrest. Treatment with Baekduong-tang led to increased expression and phosphorylation of p53 and decreased expression of CDK2 and CDK6 in HCT-116 cells. It also activated caspase-3 through caspase-10 and caspase-9 activation. Finally, Baekduong-tang induced production $H_2O_2$, superoxide anion ($O_2^-$) and NO and modulated proteins expression including SOD, NOS, Bax and Bcl-2. Conclusions : These results indicate Baekduong-tang induces apoptotic death of HCT-116 cells through G2/M phase arrest and disturbance of intracellular redox status in a p53-dependent manner.