꽃마을 한방병원에서 사용하는 약침

대한약침학회 대한약침학회 2000 Journal of pharmacopuncture Vol.3 No.1

A549 폐암세포와 Sarcoma-180 복강암에 대한 인삼(人蔘) 증류약침(蒸溜藥鍼)의 영향에 관(關)한 실험적(實驗的) 연구(硏究)

위종성,권기록,박희수,We, Jong-Seong,Kwon, Ki-Rok,Park, Hee-Soo 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.3

Objectives : In order to investigate effects and immune improvement of distilled white-ginseng herbal extract, expression of Cox-1, Cox-2, and mRNA of Bcl-2 and Bax were analyzed in A549 cell in vivo. Survival time and expression of cytokine mRNA were measured for the mice with Sarcoma-180 induced abdominal cancer. Methods : Balb/c mouse was treated with distilled white-ginseng Herbal Acupuncture at Wisu($Bl_{21}$) and Chung-wan($CV_{12}$) to investigate anti-cancer effects and immune response. Results : 1. For expression of mRNA of Cox-1 using RT-PCR. the control group and the experiment groups show significant increase. For Cox-2, both experiment groups and the normal group showed significant decrease. For Bcl-2, experiment groups showed slight decrease compared to the control group. For Bax, no significant changes were shown between the control group and experiment groups 2. For survival time, all of experiment groups didn't show significant differences. 3. IL-2 productivity using Flow cytometry, experiment group I didn't show any significance, For II-4, all of experiment groups showed slight decrease compared to the control group. 4. For IL-2 productivity using ELISA, experiment group I showed slight decrease compared to the control group, experiment group II didn't show any significance. 5. For expression of cytokine mRNA using RT-PCR, significant increase of IL-2 and IL-4 were witnessed in experiment group I compared to the control group. Significant decrease of IL-10 was shonwn in all of experiment groups compared to the control group. Conclusion : According to the results, we can expect that distilled white-ginseng Herbal Acupuncture may be further effects in anti-cancer and immune improvement if increasing concentration.

도인약침액(桃仁藥鍼液)의 투여가 흰쥐의 고지혈증 예방에 미치는 영향

강대인,정찬길,김광호,소경순,Kang, Dae-In,Jeong, Chan-Gil,Kim, Kwang-Ho,Soh, Kyeong-Sun 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.3

Objective : In order to study effects of ${\lceil}Persicae\;Semen{\rfloor}$ on the hyperlipidemia rats induced by Triton WR, we have made hyperlipidemia rats by administering Triton WR-1339 for 3 days, then have administered. The results were summerized as follows : 1. The Total cholesterol, triglyceride, LDL-cholesterol, Free fatty acid, phospholipid and CRF(Cardiak Risk Factor) by hypercholesteremic rats injected by Triton WR-1339 of the herb-Acupuncture group were decreased as compared with those of the control group, especialy hypercholesteremic rats injected by Triton WR- 1339 of the herb-Acupuncture+Acupuncture group were some more decreased as compared with those of the control group. 2. The HDL-cholesterol by hypercholesteremic rats injected by Trion WR -1339 of the herb-Acupuncture group were increased as compeared those of control group, especialy hypercholesteremic rats injected by Triton WR-1339 of the herb-Acupuncture+Acupuncture group were some more increased as compared with those of the control group. According to the above results, Persicae Semen showed inhibition on decrease of serum total cholesterol, triglyceride and phosholipid contents as well as inhibition on increase of HDL-cholesterol contents in hyperlipidemia.

분심기음약침(分心氣飮藥針)이 과산화지질(過酸化脂質)을 투여한 흰쥐의 혈장(血漿) 및 간장(肝臟)지질구성과 항산화(抗酸化)에 미치는 영향 -태충혈(太衝穴)과 내관혈(內關穴)을 중심으로-

이준무,김민기,Lee, Jun-Mu,Kim, Min-Ki 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.3

Effects of Boonsimgieum aqua-acupuncture at $Naegweun(HP_6)$ and $Taecheung(LV_3)$ on liver and plasma lipid composition and antioxidative capacity were investigated in rat fed high oxidized fat. Concentrations of plasma triglyceride, total cholesterol and LDL-cholesterol showed a tendency to increase in the high oxidized fat diet group. However these values showed a tendency to decease in aqua-acupuncture groups. HDL-cholesterol showed no significantly different in all the treatment groups. Liver total cholesterol values showed a high in control group, however other treatment groups showed no significantly different. Liver triglyceride concentration showed a high value in control group and $Naegweun(HP_6)$ aqua-acupuncture group showed lower value than $Taecheung(LV_3)$ aqua-acupuncture group. Plasma GOT and GPT values showed a tendency to increase in high oxidized fat diet group. However aqua-acupuncture groups showed a lower values than control group. The concentration of TBARS in liver and plasma showed a high values in high oxidized fat diet group, however these values showed a tendency to decrease in aqua-acupuncture group. Glutathione peroxidase, superoxide dismutase and catalase activity values showed a low values in high oxidized fat diet group, however these values showed a tendency to increase in aqua-acupuncture groups and in aqua-acupuncture groups, these values showed no significantly different.

봉독 면역요법후의 면역학적 변화에 대한 고찰 -알레르기 질환에 응용 가능성을 중심으로-

한동하,Han, Dong-Ha 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.3

Beevenom immunotherapy(BVIT) in allergic patients is a well-established treatment modality for the prevention of systemic anaphylactic reactions caused by insect stings. BVIT is accompanied by increases in allergen-specific IgG, particularly the IgG4 isotype, which blocks not only IgE-dependent histamine release from basophils but also IgE-mediated antigen presentation to T cells. Inhibition of T cells after BVIT also involves decreased induction of the costimulatory molecule ICOS, which, in turn, seems to be dependent on the presence of IL-10, also associated with the inhibited status of T cells after BVIT. Suppression of T cells by IL-10 is an active process, which depends on the expression and participation of CD28. Immune tolerance in specific allergen immunotherapy might be a consequence of decreased Th2 or increased Th1 response of allergen specific T lymphocytes. BVIT shifted cytokine responses to allergen from a TH-2 to a TH-1 dominant pattern, suggesting direct effects on T cells. Many studies showed that severe side effects due to venom immunotherapy are rare. These results suggest that immunological changes after BVIT may be applied to be therapeutic alternative of general allergic diseases including beevenom allergy.

Effects of Atractylodis Rhizoma Pharmacopuncture on an Acute Gastric Mucosal Lesion Induced by Compound 48/80 in Rats

이윤규,Seong-Chul Lim,김재수 대한약침학회 2012 Journal of pharmacopuncture Vol.15 No.1

Objectives: This study was designed to investigate the protective effects of Atractylodis Rhizoma pharmacopuncture (ARP) against acute gastric mucosal lesions induced by compound 48/80 in rats. Methods: The ARP was injected in Joksamni (ST36) and Jungwan (CV12) 1 hr before treatment with compound 48/80. The animals were sacrificed under anesthesia 3 hrs after treatment with compound 48/80. The stomachs were removed, and the amounts of gastric adherent mucus, gastric mucosal hexosamine, thiobarbituric acid reactive substances (TBARS),xanthine oxidase (XO), and superoxide dismutase (SOD) were measured. Also, histological examination were performed. Results: Gastric adherent mucus, gastric mucosal hexosamine and histological defects of gastric mucosa declined significantly after ARP treatment. Changes in gastric mucosal TBARS were also reduced by ARP treatment, but this result was not statistically significant. ARP treatment did not change the XO and the SOD activities. Conclusions: ARP showed protective effects for acute gastric mucosal lesions induced by compound 48/80 in rats. These results suggest that ARP may have protective effects for gastritis.

자연산 산삼 증류약침의 혈맥주입 전.후 혈장의 Proteom 분석

강태식,이선구,권기록,Kang, Tae-Sik,Lee, Sun-Gu,Kwon, Ki-Rok 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.3

Objectives : To observe changes in the serum proteins before and after intravenous injection of wild ginseng herbal acupuncture. Methods : Blood was collected before and after the administration of wild ginseng herbal acupuncture and only the serum was centrifuged. Then differences in the spots on the scanned image after running 2-Dimensionl electrophoresis were located and conducted mass analysis and protein identification. Results : Following results were obtained from the comparative analysis of serum proteins before and after the administration of wild ginseng herbal acupuncture. 1. 28 spots were identified before and after the administration. 2. In confirming manifestation degree, spots with more than two-times increase were 204, 803, 1505, 2205, 3105, 7104, 9001 spots, with more than one-time increase were 1101, 1302, 2013, 3009, 3010, 4002, 4009, 6706, 7103, 8006, 8101, and spots with decrease were 205, 801, 3205, 5202, 6105. 3. After conducting protein identification, proteins 205, 804, 1302, 4009, 6105, 6106 are unidentified yet, and 1101 is unnamed protein. Protein 204 is identified as complement receptor CR2-C3d, 801 as YAP1 protein, 803 as antitrypsin polymer, 1505 as PRO0684, 2013 and 3010 as proapolipoprotein, 2205 as USP48, 2403 as vitamin D binding protein, 3009 as complement component 4A preprotein, 3105 as immunoglobulin lambda chain, 3205 as transthyretin, 4002 as Ras-related protein Ral-A, 4204 as beta actin, 5202 and 7104 as apolipoprotein L1, 6704 as alpha 2 macroglobulin precursor, 7103 as complement component 3 precursor, 8006 as testis-specific protein Y, 8101 as Transferrin, 9001 as(Alpha-Oxy, Beta-(C112g)deoxy) T-State Human Hemoglobin, and 9003 as human hemoglobin. 4. Immune protein CR2-C3d, which acts against microbes and pathogenic organisms, and Antitrypsin(803), which is secreted with inflammatory response in the lungs, were increased by more than 200% after the administration of herbal acupuncture. 5. Immunoglobulin lambda chain(3105), Alpha-Oxy, Beta-(C112g)deoxy T-State Human Hemoglobin(9001), and human hemoglobin(9003) were increased by more than two-times after the administration of herbal acupuncture. 6. Proapolipoprotein(2013, 3010) and apolipoprotein(7104), key components of the HDL-cholesterol which plays an important role in preventing arteriosclerosis, were increased after the administration of herbal acupuncture. 7. Vitamin D binding protein(DBP, 2403), protecting the lung at the time of inflammatory response, was increased after the administration of herbal acupuncture. 8. Transthyretin(TTR, 3205), which is the main protein causing familial aimyloid polyneuropathy(FAP), was decreased after the administration of herbal acupuncture. 9. Ras-related protein Ral-A(4002) that controls phospholipid metabolism, cytoskeletal formation, and membrane traffic, was increased after the administration of herbal acupuncture. 10. Testis-specific protein Y(8006), which takes part in determination of the gender, was increased by more than two-times after the administration of herbal acupuncture. 11. Transferrin(8101), T-State Human Hemoblobin(9001), and Human Hemoblobin(9003) which balances the iron level in the body, were increased after the administration of herbal acupuncture. Conousion : Above results support the notion that intravenous injection of cultivated wild ginseng herbal acupuncture induce changes in serum proteins and this research can be a pioneer work in finding biomarkers.

오공약침(蜈蚣藥鍼)을 사용한 하지정종(下肢淨腫) 환자(患者) 1례(例)에 대한 임상적 연구

최회강,소기숙,김성남,임정아,이정훈,문형철,김성철,Choi, Hoi-Kang,So, Ki-Suk,Kim, Sung-Nam,Lim, Jeong-A,Lee, Joung-Hoon,Moon, Hyung-Cheol,Kim, Sung-Chul 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.2

Objectives : This study is performed for the purpose of proving the efficacy of the Oriental medicine of swollen leg. Edema is defined as an increase in the extravascular(interstitial) component of the extracellular fluid volume, which may expand by several liters before the abnormality is recognized. We treated one case of patient with a swollen leg. Methods & results : We examined DITI before treatment and after treatment. We measured the circumference of thigh and calf every morning, so we compared with the values day after day. So we found the fact that leg swelling considerably decreased after treatment for 14 days. We followed up after discharge. Conclusions : Acupuncture, Aquacupuncture and Herbal medicine is possible to be effective for treating patients with a swollen leg.

가락시장 근로자의 병증과 한의학적 치료에 대한 예비 분석

유재룡,송호섭,Yoo, Jae-Ryong,Song, Ho-Sueb 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.2

Objective : To broaden our understanding on occupational disease of market workers and to evaluate the effect of TKM treatment focusing on acupuncture and herb medicine and to promote base studies and clinical trials on occupational disease. Materials and Methods : Analysis was done on 33 Ga-Rak market workers' chart which had been selected through investigation of 1508 outpatient's chart of Kyung Won University Hospital from Jun. 1st, 2002 to May. 31th, 2003. Results : 1. Out of 33 patients, Men had more occupational diseases than women had and Most people were in their forties. 2. Major cause of the disease include repetitve bending, heavy weight lifting and overwork. 3. Past History of patients mostly include frequent lumbar sprain, periarthritis of shoulder, lumbar HNP 4. The patients with occupational disease were diagnosed as lumbar sprain, periarthritis of shoulder, lumbar HNP, degenerative spondylosis, spinal stenosis and their chief complaints were low back pain, omalgia, back pain with radicular pain. 5. The duration of treatment was mostly within a week. 6. Acupuncture, Bee Venom Acupuncture, moxibustion, Herb-medicine, extract, taping therapy, physical therapy were used as treatment methods. 7. Applied herb medicine were composed of 8 kinds of prescriptions and extract were made up of 6 prescriptions 8. Applied acupoints belonged mainly to 14 meridians 9. The treatment of herb medicine combined with acupuncture proved effective in treating the ocupational disease. Conclusion : The occupational disease of patients working in Ga-Rak market were closely related with overwork, especially with repetitive bending and heavy weight lifting and TKM treatment focusing on acupuncture combined with herb medicine was effective in treating occupational disease.

요통(腰痛) 및 요각통(腰脚痛) 환자에 있어 약침치료(藥鍼治療)의 호전도에 대한 임상적 고찰

이동현,김효수,위통순,Lee, Dong-Hyun,Kim, Hyo-Soo,Wei, Tung-Shuen 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.2

Objectives : The purpose of this study is to compare the improvement rate in managering patients who had lumbago and pain in loin & feet by herbal-acupunture therapy with conservative treatment. Methods : 40patients who invited from March. 2003 to May. 2004 with lumbago and pain in loin & feet inve, were divided into two classes. A class was managed with conservative treatment(acupuncture, herb med, negative therapy, bed rest and physical therapy) and herbal-acupuncture therapy, the other class was managed with conservative treatment only. Two classes were distributed by sex and age, therapeutic periods, radiolographic results and therapeutic results that checked with VAS(visual analog scale) and ODI(Oswestry disability index). Results : According to VAS and OBI results, A class was managed with conservative treatment and herbal-acupuncture therapy shows the improvement in controling the pain of lumbago etc. comparing with the other class.