생강(Zingiberis Rhizoma Crudus) 메탄올 추출물의 산화질소 생성 억제 효과 연구

김영훈a, 배덕성a, 김정화a, 문성민b, 백채민c, 장정호d, , 황방연e, 이종택f, 이재권a 충북대학교 과학교육연구소 2012 과학교육연구논총 Vol.27 No.2

본문 참고

항균활성 천연물질을 이용한 반추위 메탄저감용 친환경 첨가제 개발

이아름,양진호,조상범,나종삼,심관섭,김영훈,배귀석,장문백,최빛나,신수진,최낙진,Lee, A-Leum,Yang, Jinho,Cho, Sang-Buem,Na, Chong-Sam,Shim, Kwan-Seob,Kim, Young-Hoon,Bae, Gui-Seck,Chang, Moon-Baek,Choi, Bitna,Shin, Su-Jin,Choi, Nag-Jin 한국유기농업학회 2014 韓國有機農業學會誌 Vol.22 No.3

본 연구는 천연물질에서 유래한 반추위 메탄저감용 친환경 첨가제 개발을 위해 각기 다른 종균을 이용하여 발효한 세신 추출물의 항균활성, 항산화활성 및 in vitro 반추위 발효시험을 체계적으로 실시하였다. 접종된 균주들의 성장효율을 알아보기 위해 실시한 생균수측정 결과 L. curvatus NJ40 균주에서 유의적으로 높은 균주성장을 나타냈다(p<0.05). 항균활성측정 결과는 대조구 대비 L. curvatus NJ40 및 L. plantarum NJ45 균주와 발효된 세신 추출물이 병원균에 대한 항균효과를 나타내는 것으로 조사되었다. In vitro 반추위 발효실험에 세신 및 발효 세신 추출물을 적용한 결과, 휘발성 지방산 생성량 대비 반추위 메탄 저감효과가 나타났다. 특히 반추위 미생물 활력 및 사료이용 효율을 대표할 수 있는 휘발성 지방산 생성효율에 대한 부정적 효과 없이, 오히려 휘발성지방산 생성효율을 향상시키면서 반추위 메탄 저감효과를 가져올 수 있는 것으로 나타났다. The present study was conducted to investigate effective starter culture to improve biological activity of Asarum sieboldii. Antibacterial activity, antioxidant activity and reduction of enteric rumen methane production were used as criterions for biological activity. Ground A. sieboldii was added in MRS broth at 10% (w/v) and fermented by different starter cultures. Weissella confusa NJ28, Weissella cibaria NJ33, Lactobacillus curvatus NJ40, Lactobacillus brevis NJ42, Lactobacillus plantarum NJ45 and Lactobacillus sakei NJ48 were used for starter culture strains. Each starter culture was inoculated with 1% (v/v) ratio and fermentation was performed at $30^{\circ}C$ with agitation (150 rpm) for 48 h. MRS broth for the control was employed without starter culture. Then the fermentation growth was dried and extracted using ethyl alcohol. The growth of starter culture was detected at NJ40, NJ42, NJ45 and NJ48. And the highest cell growth was found in NJ40. Antibacterial activity against to Staphylococcus aureus, Listeria monocytogens, Mannheimia haemolytica and Salmonella gallinarum were observed in the extract fermented by NJ40 and NJ45. All treatments showed antioxidant activities, however, there were no significant differences (p>0.05). In in vitro rumen fermentation, negative control (NC) and positive control (PC) were assigned to without extract and with non-fermented A. sieboldii extract. Significant suppression of gas productions were detected in positive control and treatments compared to negative control (p<0.05). However, total volatile fatty acid production was not suppressed. Significant methane reduction per total volatile fatty acid productions were found in positive control and NJ45 treatment (p<0.05). The present study suggested a fermentation of A. sieboldii using NJ45 strain could improve its biological activity and make possible for its use in bio additive for enteric rumen methane mitigation without suppression of animal productivity.

시설물 입지에 있어 인구 중심점 개념을 이용한 수요 규모 추정 방법 연구

주성아 ( Sung A Joo ),김영훈 ( Young Hoon Kim ) 한국지리정보학회 2007 한국지리정보학회지 Vol.10 No.2

본 논문은 인구 중심점 개념을 이용하여 GIS 공간 모형에서 보다 정확한 수요 규모를 추정할 수 있는 방법을 제시하고자 하는 연구이다. 이를 위하여 본 연구에서는 면적 속성의 수요 데이터와 점형 속성의 인구 중심점(population centroid)의 개념을 활용하여 보다 정확한 수요 규모를 추정할 수 있는 방법을 제시하고 다양한 지역 및 공간 단위에도 적용될 수 있는 가능성을 제시하였다. 이를 위하여 기존의 수요 데이터 이용 방법의 한계와 제한점을 보완하고 보다 정확한 수요 규모의 추정을 위해서 주택 유형별 가중치 기반의 인구 중심점 추정 방법을 제시하였다. 추출된 인구 중심점을 기반으로 각 수요점의 위치와 수요 규모를 추정하고 인구 중심점과 수요 지점간의 거리 측정 방법을 통하여 실제 GIS 공간모형의 적용 가능성을 살펴 보았다. 이를 위하여 입지-배분 공간 모형을 사례로 시설물 입지를 위한 기본적인 수요 규모와 서비스 배분을 위한 GIS 공간 모형의 적용 가능성을 확인하였다. This paper is to discuss analytical techniques to estimate demand sizes and volumes that determine optimal locations for multiple facilities for a given services. While demand size estimation is a core part of location modeling to enhance solution quality and practical applicability, the estimation method has been used in limited and restrict parts such as a single population centroid in a given larger census boundary area or small theoretical application experiments(e.s. census track and enumeration district). Therefore, this paper strives to develop an analytical estimation method of demand size that converts area based demand data to point based population weighted centroids. This method is free to spatial boundary units and more robust to estimate accurate demand volumes regardless of geographic boundaries. To improve the estimation accuracy, this paper uses house weighted value to the population centroid calculation process. Then the population weighted centroids are converted to individual demand points on a grid formated surface area. In turn, the population weighted centroids, demand points and network distance measures are operated into location-allocation models to examine their roles to enhance solution quality and applicability of GIS location models. Finally, this paper demonstrates the robustness of the weighted estimation method with the application of location-allocation models.

에스테틱과 연계된 피부과의원의 디자인 아이덴티티 요소에 관한 연구

주혜라(Ju, Hye-Ra),김영훈(Kim, Young-Hoon),소현아(So, Hyun-A) 한국실내디자인학회 2009 한국실내디자인학회논문집 Vol.18 No.4

The aesthetics of current dermatology clinics are the main space of dermatology, which is gradually becoming an area of specialty. Paths requiring spatial transition due to the expansion of treatment field in dermatology clinics are currently emerging, meaning that the duty to provide medical and aesthetic environment with high spatial connection must be accompanied. Contrary to past clinics where only functional aspects were emphasized, current clinics require a differentiated environment that considers both aspects of function and aesthetics, centered on the patient. The purpose of this research in this perspective is to study the efficient connection between dermatology and aesthetics, while also analyzing identity factors to indicate design factors differentiated from other functional spaces, to apply them as preliminary data for the planning of dermatology clinics. Based on the above, six dermatology clinics located in Seoul were designated for case studies through field studies. Overall, Aesthetics connected to dermatology clinics legally must have separate business registrations and have alternative entrance ways. Currently however, there were many cases where entrance, waiting, reception and receipt were not separated. there were efforts to partially display identities when analyzing design factors that formed the identity of dermatology clinics. However, there were insufficient cases where visual communication factors such as a clinic’s spatial identity, logo, signing system, and applied products were integrated into a coherent theme. At this point when dermatology clinics are becoming brands, all fields must merge for integrated identity effects that go beyond the boundaries of contemporary H.I, to clearly display their identity with the clinics’ professional image and consistent concept.

투석이 필요했던 중증 급성 신손상 환자에서 신장기능의 예후

최경아 ( Kyung A Choi ),권정아 ( Jeong Ah Kwon ),김영훈 ( Young Hoon Kim ),이유지 ( Yu Ji Lee ),오하영 ( Ha Young Oh ),김대중 ( Dae Joong Kim ),김윤구 ( Yoon Goo Kim ),허우성 ( Woo Seong Huh ),이정은 ( Jung Eun Lee ) 대한신장학회 2009 Kidney Research and Clinical Practice Vol.28 No.4

Purpose: Acute tubular necrosis (ATN) is a serious complication in critically ill patients. This study investigated the renal outcome of severe ATN requiring RRT and prognostic factors for renal recovery. Methods: Between January 2000 and May 2008, surviving patients with presumed ATN requiring dialysis were analyzed retrospectively. Patients with pre-existing chronic kidney disease and other causes of ARF rather than ATN were excluded. Primary outcomes were complete renal recovery (CR) and dialysis withdrawal. CR was defined as a return to basal serum creatinine level or creatinine <1.5 mg/dL (male) or <1.3 mg/dL (female) without dialysis. Results: Of one hundred twenty two patients, 79 (65%) patients were male. The mean age was 54±16 years and 87 patients (71%) received continuous renal replacement therapy. 55% had ischemic ATN, 29% had septic ATN, and 16% had nephrotoxic ATN. Mean duration of dialysis was 12 (6-29) days. Dialysis withdrawal rate at 30 days and at 60 days after initiation of dialysis were 51% and 77%, respectively. CR at 60 days after initiation was 50%. Multivariate analysis revealed that older age (per year, Hazard ratio (HR)=0.981, 95% Confidence interval (CI) 0.963 to 0.999), ischemic ATN (vs. toxic ATN, HR=0.481, 95% CI 0.238 to 0.974),and longer duration of oliguria (per day, HR=0.979, 95 % CI 0.962 to 0.996) were independent prognostic factors of renal recovery. Conclusion: Young age and short duration of oliguria were favorable factors for renal recovery from ATN requiring dialysis. The cause of ATN might be also an independent prognostic factor.

신한옥형 사회복지시설 실증구축 설계 변경적용요소 분석

노진아(No, Jin-A),권오진(Kwon, Oh-Jin),김영훈(Kim, Young-Hoon) 한국생태환경건축학회 2021 한국생태환경건축학회 학술발표대회 논문집 Vol.21 No.2

In this study, we intend to review the factors for applying changes in the design of the New Han-ok Style Social Welfare facility demonstration structure and refer to it in similar fields in the future. As a method of the study, the implementation design of the demonstration structure and the construction status were compared to analyze whether the design was changed and the occurrence factors. As a result of the study, the change application factors appeared in most parts except for the main structural parts, and the occurrence factors were found to be three factors: external influence, application unpredictability, and lack of verification of existing technology.

몰약(Commiphora molmol)의 항염증 및 화장품 약리활성에 관한 연구

장영아 ( Young Ah Jang ),천순주 ( Soon Ju Cheon ),장민정 ( Min Jung Jang ),전동하 ( Dong Ha Jun ),조우아 ( Woo A Cho ),권오준 ( O Jun Kwon ),김태훈 ( Tae Hun Kim ),김영훈 ( Young Hun Kim ),이진태 ( Jin Tae Lee ) 한국미용학회 2009 한국미용학회지 Vol.15 No.3

Commiphora molmol (well known myrrha) is a reddish-brown resinous material, the dried sap of the tree Commiphora molmol, native to Yemen, Somalia and the eastern parts of Ethiopia. High quality myrrha can be identified through the darkness and clarity of the resin. Limited scientific studies suggest that myrrha has antibacterial and anti-inflammatory activities. This study determined cytotoxicity of myrrha oil to Raw 264.7 cells and its effect, measured by ELISA. This study was carried out to investigate the cosmeceutical activities of Commiphora molmol methanol extracts, by measuring electron-donating ability (using DPPH), nitrite-scavenging ability, and astringent activity. Based on the MTT assay, 12 and 24h myrrha exposures to 10ppm myrrha had little effect on Raw 264.7 cell (12h only) viability. At 24 h, 10~1000ppm myrrha decreased Raw 264.7 viability 30~38%. In nitrite-scavenging ability test, myrrha inhibited nitric oxide (NO) production and blocked LPS-induced iNOS expression. The DPPH radical scavenging activity was the 88% in 500 ppm of myrrha showed electron donating ability. Astringent effect of myrrha 98% at 500ppm concentration. These results indicate of myrrha extracts may be useful as a adjuvant for cosmeceutical industry.

조현병 환자에서 비정형 장기지속형 주사제와 경구제의 6개월 추적관찰 기간 효과비교

박찬미(Chan mi Park),박은정(Eun jung Park),이승환(Seung Hwan Lee),김영훈(Young Hoon Kim),정영철(Young Chul Chung),원승희(Seung hee Won),이상규(Sang Kyu Lee),박선철(Seon Cheol Park),배경열(Kyung ryul Bae),최진아(Jin A Choi),최하진(Ha 한국보건의료연구원 2016 근거와 가치 Vol.2 No.2

Objectives: To compare the clinical outcomes between the schizophrenia patients using long-acting injection (LAI) and those using oral agent (Oral). Methods: A prospective multicenter registry conducted at 7 hospitals comparing a one-year treatment effectiveness of LAI to reduce recurrence and to improve medication adherence (MA) and quality of life (QOL). This interim analysis presents data from the first 6 months of the follow-up period. Results: Among a total of 393 patients enrolled in this study, 385 patients (LAI 87, Oral 298) were analyzed as the interim analysis. There were no significant differences in clinical global impression (CGI), global improvement (GI), positive and negative symptom scale (PANSS), and QOL (CGI, P=0.618; GI, P=0.599; PANSS, P=0.289; EuroQol five dimensions questionnaire, P=0.254; Korean version of 4th revision of Schizophrenia Quality of Life Scale, P=0.416; visual analogue scale, P=0.581). LAI was associated with improved doctor’s perspective MA (P=0.021), but no difference was observed in patient’s perspective MA (P=0.355). Recurrence for 6 months occurred in 8 cases (9.2%) of LAI and in 43 cases (14.4%) of Oral. Kaplan-Meier analysis showed that there was no significant difference in time to recurrence after enrollment between LAI and Oral. The multivariate Cox analysis also showed no difference (hazard ratio=1.710; 95% confidence interval=0.758~3.855). Conclusion: In patients with schizophrenia experiencing recurrence, the difference of effectiveness between LAI and oral therapy could not be observed during 6 months of follow-up.

캄보디안 상황버섯 추출물의 주름개선 효과 연구

천순주(Soon-Ju Cheon),장민정(Min-Jung Jang),장영아(Young-Ah Jang),최은영(Eun-Young Choi),전동하(Dong-Ha Jun),김영훈(Young-Hun Kim),조우아(Woo-A Cho),정연숙(Yeon-Sook Jeong),권혁범(Hyeork-Bum Kwon),김태훈(Tae-Hoon Kim),최경임(Kyung-Im 한국생명과학회 2008 생명과학회지 Vol.18 No.12

사람의 피부는 자외선, 오염된 공기, 화학 제품 등 환경에 끊임없이 노출된다. 이들로 인한 자유라디칼과 활성 산소는 피부세포에 큰 손상을 끼치게 된다. 캄보디안 상황버섯 열수, 에탄올 추출물의 기능성 화장품 소재로 활용하기 위하여 지방 산패 억제능 측정과 주름개선 효과 측정을 하였다. 캄보디안 상황버섯 열수, 에탄올 추출물에 금속이온을 첨가하여 지방산패 억제능을 측정한 결과 캄보디안 상황버섯 열수 추출물 보다 에탄올 추출물이 Fe²?와 Cu²?chelating 능력이 우수하였다. 캄보디안 상황버섯 에탄올 추출물의 경우 0.1 ㎎/㎖, 0.5 ㎎/㎖, 1 ㎎/㎖의 농도에서 대조군인 butylated hydroxytoluene (BHT)보다 높은 지방산패 억제를 나타내었다. 캄보디안 상황버섯 열수, 에탄올 추출물은 산화 촉매제인 Fe²를 함유한 지방 산패 억제능이 Cu²?를 이용한 지방산패 억제능 보다 우수하였다. 주름개선 효과 측정으로 elastase 저해활성을 측정한 결과 캄보디안 상황버섯 에탄올 추출물은 0.01 ㎎/㎖의 농도에서 50.7%의 저해활성을 나타내어, 대조군인 urosolic acid보다 높은 elastase 저해활성을 타나내었다. 또한 에탄올 추출물의 경우 0.1 ㎎/㎖의 농도에서 약 50%의 저해활성을 나타내어 캄보디안 상황버섯 열수 추출물 보다 에탄올 추출물이 collagenase에 대해 높은 저해활성을 나타내었다. 이상의 결과에서 캄보디안 상황버섯의 에탄올 추출물은 지방산패 억제능이 우수할 뿐만 아니라 주름 개선 효과가 있음을 확인할 수 있었고, 주름 개선 화장품 소재로서의 가능성을 확인할 수 있었다. The skin of human is constantly being exposed to environmental irritants such as ultraviolet, smoke and chemicals. These irritants cause free radicals and reactive oxygen species which leave serious damages on the cells of skin. The water and ethanol extracts of Cambodian Phellinus linteus were investigated for the activities of anti-lipid peroxidation and anti-wrinkle effects to apply as a functional ingredient for cosmetic products. As the result of evaluation of liquid oxidation rate by add Fe²? and Cu²? to Cambodian Phellinus linteus extracts, Cambodian Phellinus linteus ethanol extracts were higher than Cambodian Phellinus linteus water extracts in the chealting ability of Fe²? and Cu²?. The Cambodian Phellinus linteus ethanol extracts exhibited that anti-lipid peroxidation higher than butylated hydroxytoluene (BHT) at the concentration of 0.1 ㎎/㎖, 0.5 ㎎/㎖ and 1 ㎎/㎖. Cambodian Phellinus linteus water and ethanol extracts showed a higher inhibitory effect on Fe²?-induced lipid peroxidation compared to Cu²?-induced lipid peroxidation. In the case of anti-wrinkle effect, the elastase inhibition activity of Cambodian Phellinus linteus ethanol extracts was 50.7%, and it is higher than urosolic acid at the concentration of 0.01 ㎎/㎖. Also, in collagenase inhibition activity, Cambodian Phellinus linteus water extract showed low effect, but Cambodian Phellinus linteus ethanol extract was about 50% at a 0.1 ㎎/㎖. concentration. These results proved that Cambodian Phellinus linteus had anti-lipid peroxidation and anti-wrinkle effect. Therefore, Cambodian Phellinus linteus could be useful as an anti-wrinkle cosmetic ingredient.

판람근(Isatis tinctoria L., 판람근(板藍根))추출물의 항산화활성 및 화장품약리활성에 관한 연구

김영훈 ( Young Hun Kim ),조우아 ( Woo A Cho ),천순주 ( Soon Ju Cheon ),장민정 ( Min Jung Jang ),성지연 ( Ji Yeun Sung ),정수현 ( Su Hyun Jung ),최향자 ( Hyang Ja Choi ),김대익 ( Dae Ik Kim ),김정옥 ( Jung Ok Kim ),이창언 ( Chang E 대한본초학회 2007 大韓本草學會誌 Vol.22 No.3