Proteomics Comparison of Longissimus Muscle between Hanwoo and Holstein Cattle

심관섭,최호성,박강희,황인호,윤창,나종삼,정현정 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.3

This study was conducted to compare proteins expressed in M. longissimus from Hanwoo and Holstein steers immediately after slaughter. Two-dimensional electrophoresis (2DE)/LC-MS/MS analysis revealed that the total number of detectable protein spots from longissimus muscle tissues was slightly higher in Hanwoo (575 ± 65) than Holstein (534 ± 13) steers, but that these numbers were not statistically significant due to large variation between replicates. A total of twelve protein spots did not match between sample groups, eight of which were expressed in the Hanwoo sample and four that were expressed in the Holstein sample. The protein spots detected in the Hanwoo sample included smooth muscle and non-muscle myosin alkali light chain 6B isomers, αB crystallin isomers, hemoglobin β-A chains, slow myosin heavy chains, and slow skeletal muscle troponin T chains. Collectively, these proteins are a class of slow-twitch muscle fiber and mirror that Hanwoo muscle tissue sampled for the current study contained more slow-twitch muscle fibers than Holstein one. Conversely, proteins detected from the Holstein sample included ankyrin repeat domain 2 and creatin kinase isomers. Given that creatin kinase isomers are related to the fast-twitch muscle, these results likely indicate that Holstein muscle tissue sampled for the current study contained more fast-twitch muscle fibers than Hanwoo beef.

Effects of Emulsified Sausage Supplemented with Ginseng Saponin on Lipid Metabolism in Rat

심관섭 한국축산식품학회 2010 한국축산식품학회지 Vol.30 No.4

The current study was designed to assess the effects of emulsified sausage supplemented with ginseng saponin on lipid metabolism by applying a rat model. Four groups of 8 rats (5 wk old) were each allocated one of 4 treatments: basal feed (C), and basal feed with 20% sausage powder containing 0% (S0), 2% (S2) and 4% (S4) ginseng saponin. The experiment was conducted for 4 wk. The results did not differ among the treatments with different amounts of sausage (ST), but daily feed intake (p<0.01) and feed conversion (p<0.001) were significantly increased in STs compared to C. Both total serum cholesterol and triglyceride concentrations were significantly (p<0.001) reduced, by 45 and 46%, and 48 and 46%, in S2 and S4, respectively, compared to S0. In the liver, the total cholesterol level was dramatically (p<0.05) decreased according to increasing sausage powder levels. In particular, S4 showed approximately 14% reduction compared to S0 (p<0.05). Liver triglyceride content also showed a similar tendency, where S2 and S4 resulted in 7% and 31% reduction. With regard to fatty acid composition in the liver tissues, palmitic acid (16:0), oleic acid (18:1), eicosanoic acid (20:1), and eicosatrienoic acid (20:3) did not differ among the STs, whereas both linoleic acid (18:2) (p<0.01) and linolenic acid (18:3) (p<0.001) showed significant increases in S2 compared to S0. The current data demonstrated that emulsified sausages supplemented with ginseng saponin effectively reduce total cholesterol and triglyceride concentrations in the serum and liver, and increase unsaturated and essential fatty acid in the liver. These data collectively imply that the sausage improved the overall lipid profile in a rat model, and can be further generalized to the result that emulsified sausage can improve lipid metabolism depending on the products’ formula.

Optimization of Aqueous Methanol Extraction Condition of Total Polyphenol from Spent $Lycium$ $chinense$ Miller to Develop Feed Additives for Pig

심관섭,나종삼,오성진,최낙진,Shim, Kwan-Seob,Na, Chong-Sam,Oh, Sung-Jin,Choi, Nag-Jin Korean Association of Organic Agriculture 2012 韓國有機農業學會誌 Vol.20 No.1

This study was conducted to develop a functional feed additive for pig with spent $Lycium$ $chinense$ Mill fruit. We investigated the optimum conditions for the extraction of polyphenol from spent $Lycium$ $chinense$ Mill using methanol. Methanol concentration as a solvent for extraction, extraction time and the volume of solvent per a gram of solid (ground spent Lyceum chinense Mill) were selected as parameters. Three levels of parameters were configured according to Box Behnken experiment design, a fractional factorial design, and total 15 trials were employed. Total polyphenol concentration from each trial was used as response from experiment system and effects of parameters on total polyphenol extraction efficiency were determined using response surface model. As a result, all terms in analysis of variance, regression ($p$ = 0.001), linear ($p$ = 0.002), square ($p$ = 0.017) and interaction ($p$ = 0.047) was significant and adjusted determination coefficient ($R^2$) was 94.7%. Total polyphenol extraction efficiency was elevated along increased methanol content and decreased solvent to solid ratio. However extraction time did not affect the efficiency. This study provides a primary information for the optimum extraction conditions to maximize total polyphenol recovery from spent Lycium chinens Mill fruit and this result could be applied to re-use of argo-industrial by-products and to develop of functional feed additives in organic farming.

B16 Melanoma 세포에서 돌나물 추출물의 멜라닌 생성 저해 효과

심관섭,김진화,이범천,이동환,이근수,표형배,Sim, Gwan-Sub,Kim, Jin-Hwa,Lee, Bum-Chun,Lee, Dong-Hwan,Lee, Geun-Soo,Pyo, Hyeong-Bae 대한약학회 2008 약학회지 Vol.52 No.3

To develop effective skin whitening agents, we tested natural herbal extracts for their melanogenic inhibitory activities. Sedum samentosum was selected for its inhibitory effect on melanogenesis in B16 melanoma cells. Ethanolic extract of S. samentosum (SSE) was evaluated for antioxidative effect and tyrosinase inhibitory activity of melanogenesis. We investigated the changes in protein level and mRNA level of tyrosinase, tyrosinase related protein (TRP)-1 and TRP-2 by using western blotting and RT-PCR, respectively. SSE showed scavenging activities of free radicals and reactive oxygen species (ROS) with the $IC_{50}$ of 342.7 $\mug/ml$ against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 64.69 $\mug/ml$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. SSE treatment suppressed the biosynthesis of melanin up to 46% and reduced tyrosinase activity up to 51% at 100 $\mug/ml$ in B16 melanoma cells. The tyrosinase activity and tyrosinase expression in B16 melanoma cells were reduced in a dose-dependent manner by SSE. Also, SSE was able to significantly inhibit tyrosinase and TRP-1 expression in mRNA level. These results suggest that SSE inhibited melanin production which may be dependent on tyrosinase activity and expression in B16 melanoma cells, and an effective whitening agent for the skin.

권백 추출물의 인간 피부 섬유아세포에서의 항산화와 Matrix Metalloproteinase-1 발현저해효과

심관섭,김진화,박성민,이범천,윤여표,표형배 대한약학회 2004 약학회지 Vol.48 No.2

The production of matrix metalloproteinases (MMPs) by the UV irradiated skin fibroblast and the degradation of extracellular matrix (ECM) by these enzymes is known as one of the main reasons of photoaging. In this paper, to investigate the relationship between aging and Selaginella tamariscina extract (STE), we investigated the effects of antioxidant and expression of UVA-induced MMP-1 in human dermal fibroblasts. STE was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the $IC_{50}$/ values of 65.1 $\mu\textrm{g}$/$m\ell$ against 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical and 40.9 $\mu\textrm{g}$/$m\ell$ against superoxide radicals in the xanthine/xanthine oxidase system, respectively. UVA induced MMP expression was reduced 75.5% by treatment with STE, and MMP-1 mRNA expression was reduced in a dose-dependent manner. Therefore STE was able to significantly inhibition of MMP expression in protein and mRNA level. All these results suggested that STE may act as an anti-aging agent by antioxidation and reducing UVA-induced MMP-1 production.

의약품의 선전

심관섭 淑明女子大學校 藥學大學 1968 藥硏 Vol.4 No.1

Structure Activity Relationship of Antioxidative Property of Flavonoids and Inhibitory Effect on Matrix Metalloproteinase Activity in UVA-Irradiated Human Dermal Fibroblast

심관섭,이범천,조호성,이재웅,김진화,이동환,김진희,표형배,문동철,오기완,윤여표,홍진태 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3

Collagenase, a matrix metalloproteinases (MMPs), is a key regulator in the photoaging process of skin due to the reactive oxygen species generated after exposure to ultraviolet A (UVA). Flavonoid compounds have been demonstrated to possess antioxidant properties, and could be useful in the prevention of photoaging. In this study, to investigate the structure-activity relationship of flavonoid compounds on their antioxidant property and inhibitory effects against the MMP activity, the effects of several flavonoids; myricetin, quercetin, kaempferol, luteolin, apigenin and chrysin, on the reactive oxygen species scavengering activity and inhibitory effect against the MMP activity were examined in vitro and in human dermal fibroblasts induced by UVA. The relative order of antioxidative efficacy, as determined using the 1, 1- diphenyl-2-picrylhydrazyl (DPPH) method and the xanthine/xanthine oxidase system, was as follows; flavones: luteolin > apigenin > chrysin, flavonols: myricetin > quercetin > kaempferol, and correlated with the respective number of OH group on their B-ring. In good correlation with the antioxidant properties, the flavonoids inhibited the collagenase activities, in a dose-dependent manner, and the MMP expression. These results suggested the UVA induced antioxidative activity and inhibitory effects of flavonoids on the collagenase in human dermal fibroblasts depends on the number of OH group in the flavonoid structure, and those with a higher number of OH group may be more useful in the prevention of UV stressed skin aging.

부화부산물 수평아리 사체를 이용한 사료용 효모 배양에 관한 연구

심관섭,박강희,김정학 한국축산환경학회 2000 축산시설환경학회지 Vol.6 No.3

수평아리 사체를 이용한 효모의 최적 배양 조건과 효모배양물이 육계의 성장에 미치는 영향을 조사하였다. 수평아리 추출물의 단백질 농도는 72시간 동안 추출되었을 때 가장 높았으며, 수평아리 사체에서 추출물을 얻기 위한 물의 첨가 비율은 수평아리 사체의 무게에 1.5배(v/w ratio)가 적당하였다. 수평아리 사체의 추출물에서 지방은 효모의 성장에 영향을 미치지 못하였다. 수평아리 사체의 추출물과 4% sugarcane molasses로 구성된 SCELP2 배지는 1% yeast extract, 2% bacto pepton 그리고 2% glucose로 구성된 YEPD 배지보다 효모수가 26% 더 증가하였다. 또한 SCELP2 배지에 4% 폐이스트를 첨가한 SBYW2 배지는 SCELP2 배지보다 효모수가 8% 증가하였다. SBYW2 배지에서 배양된 효모배양물을 5주 동안 육계에 급여한 결과 종체량은 4% 첨가구가 대조구보다 9% 증가하였다. 따라서 본 연구 결과 부화부산물로 발생되는 수평아리 사체는 사료용 효모배양물을 생산하기 위한 효모배양 배지의 질소원으로서 이용될 수 있음을 의미한다. Optimal conditions to utilize egg type male chicks from hatchery for cultivating yeast(Saccharomyces cerevisiae) and the effects of the yeast culture on growth of broiler chicks were investigated. The protein concentration of the spent cockerel extracts was the highest when extracted for 72 hours. Optimal water volume added to the spent cockerel chicks for the extraction was 1.5 times to the cockerel chicks weight (v/w ratio). Lipid in the extracts from the spent cockerel chicks did not affect on the yeast growth. The number of yeast cultured in the SCELP2 medium containing spent cockerel extracts and 4 % sugarcane molasses was higher by 26 % than that in the YEPD medium containing 1 % yeast extract, 2 % bacto pepton and 2 % glucose. Also the number of yeast cultured in the SBYW2 medium containing SCEP2 medium containing SCELP2 and 4 % brewer's yeast waste was increased by 8 %, compared to that in the SCELP2 medium. Body weight gain of chicks fed 4 % yeast culture supplementations cultivated in the SBYW2 medium was increased at 5 weeks by 9 %, relative to no supplementation(P<0.05). The results from this study suggest that the spent cockerel chicks can be utilized as nitrogen sources to produce yeast culture for animal feed.

공유숙박 소비가치와 소비위험이 공유숙박 공급 참여의도에 미치는 영향: 소비자 혁신성의 조절 효과와 함께

심관섭,양동우 한국창업학회 2019 한국창업학회지 Vol.14 No.6

This study studies the relationship between the consumption value and risk from consuming P2P(peer to peer) shared accommodation platforms such as Airbnb and the intention to participate in the supply of shared accommodation, and analyzes whether consumer innovation will be regulated in the participation of shared accommodation as a means of the main job by paying attention to the basic principle of sharing economy that can generate new income by utilizing idle resources. The results are summarized as follows. First, the economic value, cultural value, and the value of the transaction among the consumption value of shared accommodation had a significant effect on the intention of participation in the supply, but the social value did not significantly affect the intention of participation in the supply. Second, the consumption risk of shared accommodation had a significant negative effect on the intention of participation in the provision, whereas the economic risk had no significant effect on the intention of participation in the provision. Third, consumer innovation has shown that the consumption value and risk of shared accommodation do not moderate the effect on the intention of participation in the supply of shared accommodation. This study has identified thw factors that affect the intention to participate in the supply to Airbnb, a shared accommodation platform that has become a successful shared economy business model, and may be available as a useful analysis tool for future analyses targeting P2P (peer to peer) shared economy businesses in other areas. 본 연구는 유휴자원을 활용하여 새로운 소득을 발생시킬 수 있는 공유경제의 기본원리에 주목하여 공유숙박 서비스의 긍정적·부정적 소비경험이 손쉬운 부업 및 본업으로서 공유숙박 공급 참여의도에 어떠한 영향을 주는지를 분석하였다. 에어비앤비와 같은 P2P(peer to peer) 공유숙박플랫폼을 소비하면서 얻는 소비가치 및 위험과 공유숙박 공급 참여의도 간에 어떠한 관계가 있는지와 소비자 혁신성이 이 관계에 조절효과가 있을 것인가를 실증분석하였다. 연구결과, 첫째, 공유숙박의 소비가치 중 경제적 가치, 문화적 가치, 거래의 가치는 공급 참여의도에 유의한 정(+)의 영향을 주었으나, 사회적 가치는 공급 참여의도에 유의한 영향을 주지 않았다. 둘째, 공유숙박의 소비위험 중 시설·안전 위험은 공급 참여의도에 유의한 부(-)의 영향을 주었으나, 경제적 위험은 공급 참여의도에 유의한 영향을 주지 않았다. 셋째, 소비자혁신성은 공유숙박의 소비가치와 위험이 공유숙박 공급 참여의도에 미치는 영향을 조절하지 않는 것으로 나타났다. 본 연구는 성공적인 공유경제 비즈니스 모델로 자리잡은 공유숙박플랫폼 에어비앤비 등에 공급 참여하고자 하는 의도에 영향을 주는 요인들을 찾아냄으로써 향후 다른 분야의 P2P(peer to peer) 공유경제 비즈니스를 대상으로 하는 분석에도 유용한 시사점을 제공할 수 있을 것이다.

자외선이 조사된 사람 피부 섬유아세포에서 권백의 항산화와 MMP 발현에 미치는 영향에 대한 연구

심관섭,김진화,김진희,이범천,표형배 대한화장품학회 2006 대한화장품학회지 Vol.32 No.2

다양한 약용식물의 피부노화에 미치는 영향을 평가하였으며, 이 중 권백(Selaginella tamariscina)은 동양에서 암환자 치료를 위한 전통약용식물로 알려져 있다. 우리는 피부노화에 대한 화장품 소재로 권백에 대한 다양한 생물학적 평가를 하였다. 권백의 항산화 효과를 알아보기 위해 DPPH radical과 superoxide anion radical 소거효과를 측정하였다. 그 결과 DPPH radical의 IC₅₀ 값은 65.1 μg/mL이고, xanthine/xanthine oxidase에 의한 superoxide anion radical의 IC₅₀ 값은 40.9 μg/mL이었다. 세포내 활성산소 소거평가를 위해 사람 진피 섬유아세포(human dermal fibroblast)를 배양하여 UVB 20 mJ/cm²에 의해 증가된 세포내 활성산소(ROS)가 권백을 처리함으로써 활성산소 소거효과가 증가하였다. 사람 진피 섬유아세포에서 UVA에 의해 발현된 MMP-1 단백질과 mRNA가 권백에 의해 농도 의존적으로 감소하였다. 뿐만 아니라, 권백은 zymography와 semi-quantitative RT-PCR을 이용하여 UVA 조사된 사람 진피 섬유아세포에 MMP-2 (gelatinase)의 활성 감소를 확인하였다. 결론적으로 권백은 자외선에 의한 세포손상을 보호하여 항노화 화장품의 새로운 소재로 이용될 것으로 사료된다. In this study. we evaluated anti-aging activity of medical plants that protect the skin cell damage induced by UV irradiation. We have investigated diverse biological activities of Selaginella tamariscina as an anti-aging ingredient of cosmetics. S. tamariscina was found to show scavenging activities of radicals and reactive oxygen species (ROS) with the IC₅₀ values of 65.1μg/mL against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 40.9 μg/mL against superoxide radicals in the xanthine/xanthine oxidase system, respectively. For testing intracellular ROS scavenging activity, the cultured human dermal fibroblasts were analyzed by increase in dichlorofluorescein (DCF) fluorescence upon exposure to UVB 20 mJ/cm² after treatment of S. tamariscina. UVA-induced MMP-1 protein and mRNA expression in human dermal fibroblasts were reduced in a dose-dependent manner by S. tamariscina. Moreover, S. tamariscina inhibited MMP-2 (gelatinase) activity in UVA-irradiated human dermal fibroblasts assayed by zymography and semi-quantitative RT-PCR. Taken together, these results suggest that S. tamariscina may act as an anti-aging agent by Increasing collagen and preventing the skin cell damage induced by UV irradiation, and imply that S. tamariscina nay be useful as a new ingredient for anti-aging cosmetics.