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CITES 분류에 의한 사향(麝香)의 기원 동물 분류 및 특성에 대한 고찰
장문석,김도림,김현철,박성규,Chang, Mun Seog,Kim, Do Rim,Kim, Hyun Chul,Park, Seong Kyu 대한한의학방제학회 2016 大韓韓醫學方劑學會誌 Vol.24 No.4
Objectives : The purpose of this study was to investigate musk deer in taxonomic status and genus species. We investigated the characteristic of musk deer for medicinal usages. Methods : To identifications of musk deer on the taxonomic status and genus species, the literary investigation were conducted on the Korean, China, and Japan pharmacopoeia and published herbal books, CITES Species Lists too. Results : Musk deer placement in a separate family status is the Moschidae. M. chrysogaster Hodgson and M. sifanicus Przewalski was the same species. So, Alpine musk deer revised M. chrysogaster Hodgson[=M. sifanicus Przewalski]. Geographic distribution of M. moschiferus L. divided Sibirica group and himalaica group. Group himalaica contains three subspecies: Korean musk deer(M. moschiferus parvipes Hol.), Chinese musk deer(M. chrysogaster Hodgson), and Himalayan musk deer(M. leucogaster Hodgson). The genetic divergence between M. moschiferus L. and other species was clearly distinguished from the others. M. berezovskii Flerove was less than the others. However, the divergence among M. chrysogaster Hodgson, M. fuscus Li, and M. leucogaster Hodgson were quite low. Musk deers are mostly distributed around the high-plateau. Moschus were from Nepal, Bhutan, Russia, China. Forest musk deer(M. berezovskii Flerove) farming was conducted in China from 1950s. In the Korean hebal pharmacopoeia, Moschus include l-muscone($C_{16}H_{30}O$ : 238.40) over 2% for quantitative test. Conclusions : There are three species of musk deer, Siberian musk deer(M. moschiferus L.), forest musk deer(M. berezovskii Flerove), and Alpine musk deer(M. chrysogaster Hodgson) for medicinal usages.
동결 건조법을 이용한 옥천산 캡슐 제형 개발에 관한 연구
오명숙,김도림,강지웅,장문석,박선민,고병섭,박성규,Oh, Myung-Sook,Kim, Do-Rim,Kang, Ji-Ung,Chang, Mun-Seog,Park, Sun-Min,Ko, Byoung-Seob,Park, Seong-Kyu 대한한의학방제학회 2005 大韓韓醫學方劑學會誌 Vol.13 No.1
Objective : Okchun-San(OCS) is known as an effective herbal medicine on Type 2 diabetes. We performed to change OCS formulation for freeze drying capsulation. Methods The mixtures of OCS were extracted with water. finally, the filtered solution were evaporated and lyophilized to dry granules. The various ratio of excipients were studied to determine the formation for capsulizing. The samples were inspected for any difference in color, taste and appearance. Results: The prepared form of OCS were dried and weighted 260kg. The lyophilized dry powder yielding 40kg. The suitable ratio of OCS-dry powder and excipient was 10:1. The average weights of On and placebo capsules were $440{\pm}5.28mg$, $465{\pm}7.95mg$, respectively. There was no notable change in color and appearance for both capsuled samples throughout the study period. Conclusions: Therefore in can be concluded that freeze drying capsulation is appropriate form of OCS.
Hydrogen Peroxide에 의해 유도된 남성생식세포의 세포독성에 미치는 소양(銷陽)의 효과
오명숙,김도림,양웅모,장문석,박수연,강순아,박성규,Oh Myung-Sook,Kim Do-Rim,Yang Woong-Mo,Chang Mun-Seog,Park Soo-Yeon,Kang Soon-Ah,Park Seong-Kyu 대한한의학방제학회 2004 大韓韓醫學方劑學會誌 Vol.12 No.2
The purpose of this study is to examine the protective effect of Cynomorii herba on $H_2O_2$-induced cytotoxicity in male germ cells. The effects were studied by a modified MTT assay. Hydrogen peroxide significantly induced cytotoxicity in GC-1 spg cells, and co-treatment or pre-treatment of Cynomorii herba extract has reduced the cytotoxicity in a dose dependant manner. These results suggest that Cynomorii herba extract increases the survival rate of GC-1 spg cells through the antioxidant effect against $H_2O_2$-induced cytotoxicity.
右歸飮이 hydrogen peroxide에 의해 유도된 Leydig cell에 미치는 항산화 효과 연구
김수현,김도림,장문석,박성규,Kim, Soo Hyun,Kim, Do Rim,Chang, Mun Seog,Park, Seong Kyu 대한한의학방제학회 2015 大韓韓醫學方劑學會誌 Vol.23 No.1
Objectives : The purpose of this study was to investigate the antioxidant activity of water extract of Woogyuyeum (WGY) in Leydig cells. Methods : We investigated the cytoprotective effect of WGY in cultured mouse Leydig cells by MTT assay. Leydig cells treated with WGY were incubated in the presence or absence of 50 μM hydrogen peroxide at 37℃ for 24 h. The protective effects of WGY against hydrogen peroxide-induced oxidative stress, lipid peroxide (LPO), superoxide dismutase (SOD), and catalase activity assays were performed in Leydig cells. Results : As a result, WGY showed no significant cytotoxicity in Leygdig cells. WGY showed cell viability as 103.65% in 5 μg/ml concentrations. The cytotoxicity induced by hydrogen peroxide in Leygdig cells, the antioxidant effects of WGY was increased in 1, 5, 50, 100 ug/ml concentraions. 100 μg/ml concentration of WGY showed maximum antioxidant effects. Treatment of cells with 100 μg/ml WGY significantly reduced the MDA concentration to 0.23 nmoles/mg protein. SOD activity was increased at 1, 100 μg/ml concentration of WGY and catalase activity was significantly increased at 50, 100 μg/ml concentrations of WGY, respectively. Conclusions : In conclusion, WGY has antioxidant activities against hydrogen peroxide-induced oxidative stress in Leydig cells.
DPPH 방법을 통한 토사자, 보골지(補骨脂), 사상자(蛇床子), 음양곽(淫羊藿)의 항산화 활성에 대한 연구
오명숙,김도림,강지웅,김산웅,유태원,박정열,김동민,박완수,장문석,박수연,박성규,Oh, Myung-Sook,Kim, Do-Rim,Kang, Ji-Ung,Kim, San-Woong,Yu, Tae-Weon,Park, Jung-Yeul,Kim, Dong-Min,Park, Wan-Su,Chang, Mun-Seog,Park, Soo-Yeon,Park, Seong-Ky 대한한의학방제학회 2005 大韓韓醫學方劑學會誌 Vol.13 No.2
The present study was conducted to compare antioxidant activity of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba by DPPH radical scavenging activity. The extract was studied using diphenyl-picryl-hydrazyl (DPPH) for DPPH method. DPPH radical scavenging activity was measured after. .10, 20 and 30 minutes. The extract was tested by 1, 5, 10, 50, 100, 500 and $1000{\mu}g/ml$ concentrations. The results showed that the extract scavenged DPPH radical with time-dependent manner. Also, the extract showed dose-dependent DPPH radical scavenging activity. The extract of Cuscutae Semen, Psoraleae Fructus, Cnidii Fructus and Epimedii Herba scavenged DPPH radical with the IC50 being 2.7, 3.2, 2.9 and 1.1 mg/ml, respectively. In conclusion, the extract of Epimedii Herba, Cuscutae Semen, Cnidii Fructus and Psoraleae Fructus have antioxidant activity for the treatment of male sterility.
쇄양(鎖陽)의 Diphenyl-picryl-hydrazyl (DPPH) 소거 활성 및 HepG2 세포에 대한 항산화 효과
장문석,양웅모,김도림,박은화,박수연,박성규,Chang, Mun-Seog,Yang, Woong-Mo,Kim, Do-Rim,Park, Eun-Hwa,Park, Soo-Yeon,Park, Seong-Kyu 대한한의학방제학회 2007 大韓韓醫學方劑學會誌 Vol.15 No.2
The purpose of this study was to investigate the anti-oxidant effect of Cynomorium songaricum. The extract of Cynomorii Herba was studied for diphenyl-picryl-hydrazyl (DPPH) radical scavenging activity, HepG2 cell viability and $H_2O_2$-induced cytotoxicity by a modified MTT assay. DPPH radical scavenging activity was measured after 30 minutes. The extract was tested by 1. 5, 10, 50, 100 and 500 ${\mu}g/ml$ concentrations. HepG2 cell viability by a modified MTT assay was measured in the concentrations of 10, 50, 100, 250, 500 ug/ml for 24 h. The results showed that the extract scavenged DPPH radical up to 52.2% with 50 ug/ml concentration. The extract did not reduced the cell viability and $H_2O_2-induced$ cytotoxicity (69.4%) was blocked by the extract in the concentrations of 50, 100, 250 and 500 ${\mu}g/ml$. In conclusion, the extract of Cynomorii Herba has potent antioxidant activity.
Leydig Cell의 항산화에 미치는 벌사상자와 사상자의 비교연구
오지훈 ( Ji Hoon Oh ),김도림 ( Do Rim Kim ),박수연 ( Soo Yeon Park ),장문석 ( Mun Seog Chang ),박성규 ( Seong Kyu Park ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.6
Objectives : The purpose of this study was to estimate the antioxidant activity of water extract of Cnidii Fructus (CF) and Torilis Fructus (TF) in Leydig cells. Methods : Free radical scavenging activity of CF and TF against 2,2-diphenyl-1-picrylhydrazyl (DPPH) was determined spectrophotometrically. We investigated the effect of CF and TF in Leydig cells by MTT assay. The protective effects of CF and TF against hydrogen peroxide-induced oxidative stress in Leydig cells. Superoxide dismutase (SOD), and catalase activity assays were performed in Leydig cells. Results : The results showed that CF scavenged DPPH radical in a dose-dependent manner by up to 81.2%, TF scavenged DPPH radical in a dose-dependent manner by up to 63.8%. CF showed cell viability as 121.0, 132.7, 126.6% in 5, 10, 100 μg/ml concentrations. TF showed cell viability as 127.5, 111.8% in 5, 100 μg/ml concentraions, respectively. The hydrogen peroxide-induced cytotoxicity of Leydig cells were protected to 86.3% by CF at concentration of 10 μg/ml and protected to 83.5% by TF at concentration of 100 μg/ml. Both CF and TF at all concentrations, SOD activity was not significantly changed. Catalase activity was significantly increased at 10, 100 μg/ml concentrations of CF, respectively. TF`s catalase activity showed no significant difference from that of the control. Conclusions : These results suggest that CF, as an antioxidant, protects Leydig cells in hydrogen peroxide-induced oxidative stress. know that『Kwangjebikeup』played a role in settlement and spreading of foreign knowledge to civilians.
인삼(人蔘)이 수컷 생쥐의 정자 수와 CatSper3, 4 단백질 발현에 미치는 영향
박은화 ( Eun Hwa Park ),김도림 ( Do Rim Kim ),박성규 ( Seong Kyu Park ),장문석 ( Mun Seong Chang ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.2
Objectives:The purpose of this study was to investigate the reproductive effect of Ginseng radix on male mice. Methods:Male C57BL/6J mice were divided into four groups; normal group (vehicle-treated, n=8), Ginseng radix treatment group (100, 500, 1000 mg/kg, n=8). Ginseng radix extract was treated for 5 weeks. After treatment each group was examined for assessment of sperm count and CatSper protein expression using computer assisted semen analysis (CASA) system and the immunofluorescence. Results:Sperm count of normal and Ginseng radix extract treated group were 287.57 vs. 371.62, 364.83, 343.29 x106, respectively. The CatSper3, 4 proteins expression of Ginseng radix treated group were significantly increased than that of normal group. Conclusions:These findings suggest that the Ginseng radix improves male reproductive function by increasing sperm count and CatSper protein expression.