시판되고 있는 까칠복 ( Fugu stictonotus ) 의 독성

김경찬,박진우,이명자,김상록,김동수,김현대,박영호 ( Kyung Chan Kim,Jin Woo Park,Myung Ja Lee,Sang Rok Kim,Dong Soo Kim,Hyun Dae Kim,Yeung Ho Park ) 한국수산과학회 1995 한국수산과학회지 Vol.28 No.1

Ten specimens (5 males and 5 females) of the pufferfish, Fugu stictonotus ($quot;Ggachilbog$quot;), were collected at a fish market of Pusan, Korea in july 1993, and examined for anatomical distribution of toxicity by mouse assay method. The frequency of toxic specimens was 40% for liver, 60% for ovary, 40% for skin and 60% for bile in female puffers. The highest toxicities were 107, 107, 29 and 93MU/g for liver, ovary, skin and bile, respectively; and average toxicity ±S.E, values were 14±11, 48±22, 4 ±3 and 12±9MU/g, respectively. The range of total toxicity was shown to be from 0 to 35,316MU. The characteristic pattern of toxin distribution observed on these specimens was exhibited; both muscle and testis were non-toxic, but others were weakly toxic. Also, there was significant difference for toxicity between male and female specimens.

HL-60 세포에서 $TNF-{\alpha}$에 의한 MCP-1 발현에 미치는 Betulinic Acid의 효과

김경찬,이추희,Kim, Kyung-Chan,Lee, Chu-Hee 대한약학회 2008 약학회지 Vol.52 No.1

Betulinic acid, a naturally occurring pentacyclic triterpenoid, is found in abundance in the outer bark of white birch (Betula alba). In this study, we investigated if betulinic acid affects cytokine expression from activated macrophage cells. ELISA result showed that stimulation of HL-60 cells with proinflammatory cytokine such as $TNF-{\alpha}$ resulted in MCP-1 release into culture medium. In addition, transcriptional upregulation of MCP-1 in response to $TNF-{\alpha}$ was observed by RT-PCR analysis. However, incubation of HL-60 cells with betulinic acid prior to $TNF-{\alpha}$ treatment abrogated MCP-1 expression in transcription and translational level. Consistent with a number of studies which reported requirement of ERK activation for $TNF-{\alpha}$ expression, Western blot analysis showed that $TNF-{\alpha}-induced$ ERK activation was suppressed by pretreatment of HL-60 cells with betulinic acid. Taken together, our data indicate that betulinic acid exerts its anti-inflammatory effect through inhibition of $TNF-{\alpha}-induced$ ERK activation which is required for the subsequent MCP-1 release.

초정밀작업을 위한 6자유도 마이크로 스테이지의 개발

김경찬,김수현,곽윤근,Kim, Kyung-Chan,Kim, Soo-Hyun,Kwak, Yoon-Keun 대한기계학회 1998 大韓機械學會論文集A Vol.22 No.2

A new 6 degrees-of-freedom micro stage, based on parallel mechanisms and actuated by using piezoelectric elements, has been developed for the application of micro positioning such as semiconductor manufacturing devices, high precision optical measurement systems, and high accurate machining. The micro stage structure consists of a base platform and an upper platform(stage). The base platform can effectively generates planar motion with yaw motion, while the stage can do vertical motion with roll and pitch motions with respect to the base platform. This separated structure has an advantage of less interference among actuators. The forward and inverse kinematics of the micro stage are discussed. Also, through linearization of kinematic equations about an operating point on the assumption that the configuration of the micro stage remains essentially constant throughout a workspace is performed. To maximize the workspace of the stage relative to fixed frame, an optimal design procedure of geometric parameter is shown. Hardware description and a prototype are presented. The prototype is about 150mm in height and its base platform is approximately 94mm in diameter. The workspace of the prototype is obtained by computer simulation. Kinematic calibration procedure of the micro stage and its results are presented.

BF₃LiMA를 단량체로 하는 고체 고분자전해질 합성과 전기화학적 특성

김경찬,류상욱,Kim, Kyung-Chan,Ryu, Sang-Woog 한국전기화학회 2011 한국전기화학회지 Vol.14 No.4

합성된 $BF_3LiMA$ 리튬염을 단량체로 사용하는 고체 고분자전해질을 제조하고 $BF_3LiMA$의 농도가 이온전도도에 미치는 영향 및 전기화학적 안정성을 교류임피던스 측정법과 선형전위주사법을 통하여 평가하였다. 그 결과 $BF_3LiMA$가 12.9 wt%인 고체 고분자전해질에서 $7.71{\times}10^{-6}S\;cm^{-1}$의 가장 높은 $25^{\circ}C$ 이온전도도가 관찰되었으며 이 값을 전후로 이온전도도는 다소 감소하는 경향이 나타났다. 이러한 결과는 저농도의 $BF_3LiMA$에서 발생할 수 있는 리튬염의 부족과 고농도의 $BF_3LiMA$에서는 발생할 수 있는 고분자기질의 유동성 감소가 원인으로 해석된다. 또한 $BF_3LiMA$ 기반의 고체 고분자전해질은 음이온이 고정되어 있는 자기-도핑형 계열로서 $60^{\circ}C$에서 6.0 V까지 우수한 전기화학적 안정성을 보여주었다. Solid polymer electrolytes using $BF_3LiMA$ as monomer were synthesized by usual one step radical polymerization in THF solvent. The effect of $BF_3LiMA$ concentration on ionic conductivity and electrochemical stability was investigated by AC impedance measurement and linear sweep voltammetry. As a result, the highest ionic conductivity reached $7.71{\times}10^{-6}S\;cm^{-1}$ at $25^{\circ}C$ was obtained in 12.9 wt% of $BF_3LiMA$ content. Further increase or decrease of $BF_3LiMA$ content result to decrease the ionic conductivity due to the brittle matrix properties in former case and the insufficient number of charge carrier in the latter case. Furthermore, since the counter-anion was immobilized in the self-doped solid polymer electrolytes, high electrochemical stability up to 6.0 V was observed even in $60^{\circ}C$.

자기-도핑형 poly(PEGMA-co-BF₃LiMA) 전해질의 합성과 이온전도도에 대한 PEGMA분자량의 영향

김경찬,류상욱,Kim, Kyung-Chan,Ryu, Sang-Woog 한국전기화학회 2012 한국전기화학회지 Vol.15 No.4

분자량이 각각 300(PEGMA300) 및 1100(PEGMA1100) g $mol^{-1}$인 PEGMA와 합성된 $BF_3LiMA$ 리튬염을 이용하여 다양한 조성의 고분자전해질을 제조하고 전기화학적 특성을 평가하였다. 흥미롭게도 AC-impedance 측정법에 의한 상온 이온전도도는 분자량 $300g\;mol^{-1}$로 합성된 액체 고분자전해질에서 $8.54{\times}10^{-7}S\;cm^{-1}$의 값이 얻어진 반면, PEGMA1100으로 합성된 고체상태의 고분자전해질에서 최대 14배 이상 높은 $1.22{\times}10^{-5}S\;cm^{-1}$가 관찰되었다. 이러한 결과는 PEGMA에 ethylene oxide 단위가 5개인 $300g\;mol^{-1}$보다 23개인 $1100g\;mol^{-1}$에서 리튬이온의 배위가 쉽게 일어나기 때문으로 해석된다. 또한 양이온 수율 측정결과 리튬메탈과 $BF_3$간의 반응으로 인해 0.6의 비교적 낮은 값이 나왔지만 초기 3000초 동안에는 0.9 이상의 값이 관찰되어 단일이온 전도체의 특징을 보여주었다. Polymer electrolytes consisted of $BF_3LiMA$ and 300 (PEGMA300) or 1100 (PEGMA1100) g $mol^{-1}$ of PEGMA were prepared and the electrochemical properties were characterized. Interestingly, the AC-impedance measurement shows $1.22{\times}10^{-5}S\;cm^{-1}$ of room temperature ionic conductivity from PEGMA1100 based solid polymer electrolytes while $8.54{\times}10^{-7}S\;cm^{-1}$ was observed in PEGMA300 based liquid polymer electrolytes. The more suitable coordination between lithium ion and ethylene oxide (EO) unit might be the reason of higher ionic conductivity which can be possible in PEGMA1100 based electrolytes since it has 23 EO units in monomer. The lithium ion transference number was found to be 0.6 due to the side reactions between $BF_3$ and lithium metal expecially for longer time but 0.9 was observed within 3000 seconds of measuring time which is strong evidence of a single-ion conductor.

펨토초 레이저를 이용한 미세 연소노즐 가공 기술

김경찬(Kyung Chan Kim),김경호(Kyung Ho Kim),하지수(Ji Soo Ha),손익부(Ik Bu Sohn),최해운(Hae Woon Choi),김태권(Tae Kwon Kim) Korean Society for Precision Engineering 2010 한국정밀공학회지 Vol.27 No.11

Churg-Strauss 증후군에 동반된 Wells` Syndrome

김경찬 ( Kyung Chan Kim ),정현 ( Hyun Chung ),박준수 ( Joon Soo Park ),박재복 ( Jae Bok Park ),최정윤 ( Jung Yoon Choe ),박성훈 ( Sung Hoon Park ),김지영 ( Ji Young Kim ),김성규 ( Seong Kyu Kim ) 대한류마티스학회 2008 대한류마티스학회지 Vol.15 No.1

Wells` syndrome is an inflammatory dermatosis with associated aberrant eosinophil responses caused by unknown factors. Its histology is characterized by erythematous plaques with flame figures in the dermis, which is potentially diagnostic but not pathognomic. Cases of Wells` syndrome in patients with Churg-Strauss syndrome (CSS), which is characterized by antineutrophil cytoplasmic antibody-related necrotizing vasculitis, marked peripheral eosinophilia, and eosinophil tissue infiltrates, have rarely been reported, and the pathogenic association between these two diseases remains undetermined. Differences of clinical and histopathologic features of these two diseases suggest that they are distinct disease entities, even though, in part, they share pathogenic mechanisms. Here we present a new case with Wells` syndrome in a patient with CSS, treated with systemic steroid.

폐루프 구조를 가지는 6 자유도 머니퓰레이터의 개발 및 기구학적 해석

김경찬(Kyung-Chan Kim),우춘규(Chun-Kyu Woo),김수현(Soo-Hyung Kim),곽윤근(Yoon Keun Kwak) Korean Society for Precision Engineering 1998 한국정밀공학회지 Vol.15 No.1

543 nm 헬륨네온 레이저의 주파수 안정화를 위한 I<SUB>2</SUB> 포화흡수분광

김경찬(Kim, Kyung-Chan),엄년식(Eum, Nyeon-Sik),홍주현(Hong, Joo-Hyun),서호성(Seo, Ho-Sung) 한국산학기술학회 2010 한국산학기술학회논문지 Vol.11 No.3

본 연구는 543 nm 헬륨네온 레이저의 주파수 안정화를 위한 <SUP>127</SUP>I2 포화흡수분광 장치의 설계와 제작에 관한 것이다. 온도와 PZT 변화에 의한 레이저관 길이 가변을 이용하여 543 nm 헬륨네온 레이저의 주파수를 주사시켰다. 3 차 고조파 신호을 얻기 위해 PZT 변조 가능한 레이저 출력경을 이용하여 주파수 변조를 하였다. 그 결과, <SUP>127</SUP>I2 용 B-X 시스템의 R(12) 26-0에서 R(106) 28-0의 천이를 가지는 초미세구조 분광은 포화흡수분광기를 사용한 3차 미분 신호에서 관찰되었다. 또한, 전형적인 초미세구조 분광 신호의 신호대 잡음비는 약 30:1 로 얻어졌다. We have designed and assembled a saturated absorption-spectroscopy system of <SUP>127</SUP>I2 for the frequency stabilization of the 543 nm He-Ne laser. The frequency of a internal-mirror 543 nm He-Ne laser was swept by utilizing the temperature and PZT change of the laser cavity length. Frequency modulation was applied to the output laser mirror with a PZT modulator for the third harmonic signal. A portion of the hyperfine spectrum originated from the R(12)26-0 and R(106)28-0 transitions of the B-X system of <SUP>127</SUP>I2 was observed as the third-derivative signal using a saturated absorption spectroscopy. The signal-to-noise ratio of a typical hyperfine spectrum signal was estimated to be 30:1.

진행성 비소세포폐암 환자에서 제1차 요법으로서 매주요법 Docetaxel/Platinum 복합항암화학요법의 효과

김소연 ( So Yeon Kim ),류헌모 ( Hun Mo Ryoo ),배성화 ( Sung Hwa Bae ),정현영 ( Hyun Young Jung ),김경찬 ( Kyung Chan Kim ),현대성 ( Dae Sung Hyun ),이상채 ( Sang Chae Lee ),김경옥 ( Kyeong Ok Kim ),이경희 ( Kyung Hee Lee ),현명수 대한내과학회 2007 대한내과학회지 Vol.72 No.6

목적: 비소세포폐암은 최근 지속적으로 증가하며 대다수의 환자가 질병 경과 중에 항암화학요법이 필요하다. Docetaxel과 platinum의 복합항암화학요법이 비소세포폐암의 치료에 효과적이지만 표준적인 3주요법은 심각한 골수 억제 독성이 있다. 주 단위 저용량의 docetaxel을 투여할 경우 효과를 유지하면서 골수 억제 독성을 감소시킨다고 한다. 이에 저자들은 진행성 비소세포폐암 환자에서 주 단위 저용량 docetaxel과 platinum 복합항암화학요법의 효과와 독성을 알아보기 위해 본 연구를 시행하였다. 방법: 2004년 7월부터 2005년 12월까지 총 29명의 완치가 불가능한 비소세포폐암 환자를 대상으로 docetaxel 35 mg/m2를 제 1, 8, 15일에 1시간 동안 정맥점적주사하고, 제1일에 cisplatin 75 mg/m2 (60세 미만) 또는 carboplatin (AUC 6, 60세 이상)을 docetaxel 투여 후 정맥점적주사하였다. 결과: 총 29명 중 남자 25명, 여자 4명이었고, 중앙 연령 66세, 60세 이상 65.5%, 일상생활수행능력(ECOG) 2가 55.2%였다. 편평상피세포암 48%, 선암 38%였다. 제IIIB병기 21%, 제IV병기 72%, 수술 후 재발한 환자 7%였다. 복합항암화학요법은 총 95회를 투여하였다. 평가 가능한 26명에서 부분반응 44.8%, 안정성 병변 10.3%, 진행성 병변 34.5%로 전체종양반응률 44.8%였다. 중앙 생존기간 11.3개월(95% 신뢰구간, 23-67주), 무진행생존기간 5.3개월(95% 신뢰구간, 11-31주), 1년 추정 생존률 37%였다. 연령, 성별, 병기, 일상생활수행능력 및 조직학적 아형에 따른 생존률은 통계학적으로 의미있는 차이는 없었다. 결론: 진행성 비소세포폐암 환자에서 저용량의 docetaxel 매주요법과 platinum 제제의 복합항암화학요법은 3주요법과 대등한 전체 종양 반응률과 중앙생존기간을 보이면서 낮은 혈액학적 독성을 보여 일상생활수행능력이 불량하거나 고령인 환자를 포함하여 비소세포폐암의 새로운 치료법이 될 수 있을 것으로 생각한다. Background: Docetaxel is a highly effective chemotherapeutic agent with proven efficacy for non-small cell lung cancer (NSCLC). However, myelosuppression can be a substantial concern when docetaxel is administered every 3 weeks. Weekly administration of low-dose docetaxel has demonstrated a comparable efficacy together with a distinct toxicity profile with reduced myelosuppression. We conducted a phase Ⅱ study of weekly administration of docetaxel and cisplatin or carboplatin in patients with advanced NSCLC to evaluate efficacy and safety. Methods: Twenty-nine patients with advanced or metastatic NSCLC who had not received prior treatment were enrolled in the study. The patients received intravenous infusions of docetaxel (35 mg/m2 on days 1, 8, 15) and cisplatin (75 mg/m2 on day 1) or carboplatin (AUC 6), followed by a week of rest. Results: Twenty-six patients were assessable for efficacy and all patients were assessable for toxicity determination. The overall response rate of the regimen was 44.8%. The median survival was 11.3 months, and the 1-year survival rate was 37%. Of the hematologic toxicities, grade 3/4 neutropenia were observed in 12.6% of the patients, but there were no episodes of neutropenic fever. Non-hematologic toxicities were mild. Conclusions: With this weekly dosing regimen, although efficacy is comparable, myelosuppression is substantially less, and the overall tolerability profile is better than with dosing every 3 weeks. (Korean J Med 72:625-631, 2007)