高濕度下에 있어서의 마른김의 成分變化에 關한 硏究

朴榮浩 釜山水産大學校 1973 釜山水産大學 硏究報告 Vol.13 No.1

In the present study, the effect of storage condition, particularly, high humidity to the changes in chemical components of dried lavers was investigated and some characteristic patterns of the changes were discussed. Dried lavers, Porphyra yezoensis Ueda, were stored at 20� under two different levels of humidity, that were 78% and lower than 7% as relative humidity, respectively. And the content of pigments, organic acids, sugars, and fatty acids were analyzed at 10,25 and 45 day intervals during the storage. Total acidity was also determined. Degradation of chlorophyll a was faster than other pigments such as carotenoids and phycobilins under high humidity. For instance, after 45 day storage, more than two-fold of chlorophll a was retained in low humidified sample compared with that in the case of high humidity whereas a remarkable difference in retention rate of carotenoids or phycobilins was not detected. phycobilins showed high stability under the condition of high humidty, particularly, in phycoerythrin than in phycocyanin. Eight kinds of non-volatile organic acid namely pyruvic, lactic, malonic, fumaric, oxalic, malic, succinic, and citric acid were gas chromatographically identified. The stability of theses acids appeared quite differently by storage humidity. The acids like oxalic, malic, and succinic, did not show an outstanding change under low humidity even after 45 day storage while both oxalic and malic acids remarkably decrased under high humidity and succinic acid contrary increased under the same condition. Citric acid, however, decreased under both cases of humidity. The total amount of volatile acid tended to increase throughout the storage under both high and low humidity, but the rate of increase was relatively greater in case of high humidity than that of low humidity. On the other hand, the same tendedcy was observed in change of total acidity in which the value tended to increase under the high humidity while that remained steady under the low humidity. In the free amino acid composition of dried lavers, alanine,glutamic and aspartic acid were dominant in content whereas such amino acids as methionine, cystine, histididine, comparatively low. It is noteworthy that the content of taurine was tremendously high as an amino acid related compound. During the storage, a significant change in the total amino acid level and individual variation was not detected. In another aspect of storage stability of chemical components, glucose remained with negligible change under both storage conditions and galactose increased rapidly under the high humidity while maintained a steady level under the low humidity. On the contrary, the floridoside decreased rapidly under the high humidity whereas slowly under the low. In the tendecy of strorage stability of fatty acid, tended to decrease in the low humidified material. Under high humidity, ?? acid appeared stable while ?? acid considerably increased. The total fatty acid level which was consisted of 14 kinds of fatty acid seemed to increase in high humidified material and to decrease in the low humidified material.

폴리에스터 연속 중합 공정의 전산모사 연구

박융호,강우철 漢陽大學校 工學技術硏究所 1999 工學技術論文集 Vol.8 No.1

Process modeling, though a very useful tool in optimizing chemical processes, is not utilized very much in Korea due to lack of modeling techniques and application experiences. Modeling of the complicated processes haven't been attempted very much due to the difficulties in using the process simulator and the lack of actual experiences in optimization of large processes. In the case of continuous process of PET polymerization, the modeling of the entire process has never been reported due to secret of commercial processes. In this works, a model for PET process has been bullit with a commercial simulator(Aspen Plus) with a detailed consideration of undissolved TPA particles in esterification step and mass transfer of EG in polycondensation step. The model was used in investigating the effect of the changes in the operating conditions of the process on the properties of polymer product and in studying the interaction among the process equipments. And practical techniques for process modeling and optimization have been suggested through the example of PET process modeling.

Ethylene 중합용 Pentamethylene Bridged Dinuclear Zirconocene 합성에 관한 연구

박융호,임선화 漢陽大學校 工學技術硏究所 2001 工學技術論文集 Vol.10 No.-

본 연구는 메탈로센 신촉매 공정 개발을 위하여 진행되었으며, 하나의 촉매에 두 개의 활성점을 갖는 pentamethylene bridged dinuclear zirconocene을 Noh 등의 protocol을 활용하여 합성하였고, 상업화시 가장 중요한 변수인 반응 수율을 향상시키기 위하여 각 합성 단계의 개선을 시도하였다. Biscyclopentadienyl pentane 합성시 silica gel chromatography column을 이용하여 순수한 ligand를 제조하였고, trimethyltin compound 합성시 용매 변환과 TEA 주입을 통하여 수율을 향상시켰다. 최종 메탈레이션 반응시에는 반응 온도의 변화를 통하여 촉매의 활성을 향상시킬 수 있었다. This study was done for the development of a new metallocene catalyst process. The pentamethylene bridged dinuclear zirconocene which have two active sites in one catalyst had been synthesized using Noh et al's protocol and each synthetic step was modified for the improvement of reaction yield which was the most important variable in commercialization. Pure biscyclopentadienylpentane was obtained by passing through silica gel chromatography column and the reaction yield of trimethyltin compound was improved by solvent change and TEA injection. A change in the reaction temperature during the final metallation process also improved the catalytic activity.

명태 肉質의 冷凍變性 防止에 關한 硏究

朴榮浩,姜泳周 釜山水産大學校 1974 釜山水産大學 硏究報告 Vol.14 No.1

冷凍명태 fillet의 冷凍變性 防止에 미치는 各種 添加物處理의 效果를 試驗코져 fillet를 凍結前에 21種類의 處理液에 5分間 浸漬하여 凍結貯藏한 후 解凍하였 을때의 free drip, expressible drip의 發生量 및 drip中의 total solid, nitrogen, DNA의 含量을 定量하여 冷凍變性의 程度를 比較檢討하였다. 1. Free drip는 處理液의 種類 및 濃度에 따라 큰 差를 나타내었으나, expressible drip에 있어서는 그 差가 적었다. 2. Drip량의 減少에 있어 效果가 큰 處理液은 sodium polyphosphate 10%, sodium polyphosphate: sodium pyrophosphate(1:1) 10% 및 5%, sodium chloride 10%, sodium citrate 10%였다. Sodium metaphosphate, glucose, sucrose 및 sorbitol의 處理 效果는 그다지 크지 못하였고, malic acid 處理는 큰 逆效果를 나타내었다. 3. Drip中의 total solid 및 nitrogen은 大체的으로 drip量에 比例하는 結果를 보였으나, malic acid 處理區의 nitrogen量은 drip의 發生量에 비하여 相當히 적은 量을 나타내었다. 4. Drip中의 DNA量에 미치는 前處理의 效果는 drip發生量 및 drip中의 total solid, nitrogen의 경우 보다 현저하였고, 特히 重合燐酸鹽의 效果는 커서 sodium metaphosphate까지도 良好한 結果를 나타내었다. Malic acid 處理區는 drip量은 월등히 많은데도 DNA 含量은 微量에 지나지 않았다. The present study was conducted to evaluate the effect of prefreezing treatment, dipping the fillets into such solutions as condesed phosphate, sugars, and sodium chloride, upon the freezing denaturation of Alaska pollack muscle. The degree of freezing denaturation was estimated by measuring the amount of drip released, contents of total solid, total nitrogen, and DNA in the drip. Fillets were frozen at -27℃- -28℃ and stored for 20 and 40 days at -18℃- -20℃ . The amount of free drip was greatly dependent on treating solution although the expressible drip was not so much variable. Among the treating solutions examined, 10% sodium polyphosphate, 5% and 10% of the mixture of sodium polyphosphate and sodium pyrophosphate (1:1 w/w), 10% sodium chloride, and 10% sodium citrate appeared most effective ones to retard the releasing of drip. The effect of sodium metaphosphate, glucose, sucrose, and sorbitol was not so benefitable. On the contrary, malic acid showed a remarkable reverse effect. As a tendency, total solid, total nitrogen, and DNA in the drip varied proportionally to the amount of drip released. Releasing of DNA, however, was greatly affected by treating solutions comparing with that of total solid and total nitrogen. It was not the case in malic acid treated fillet particularly in DNA content, even though the amount of drip was greater than other cases, DNA content was far less.

에탄올 탈수 PSA 공정의 전산 모사 및 설계 최적화 연구

박융호,최재식 漢陽大學校 工學技術硏究所 1997 工學技術論文集 Vol.6 No.1

A model for scale-up of PSA process of ethanol dehydration was established using bench experimental data. LDF mass-transfer model, Langmuir adsorption isotherm and single-component adsorption were assumed in the model and the coefficients for heat and mass transfer in the beds were fitted against the experimental data. Model equations were solved using a DAE solver (Speed Up). The effects of cycle time, P/F ratio, adsorption pressure, bed length were examined, and the dimension and the operating condition which give optimum peformance for a commercial process were determined.

백일해 백신이 Streptozotocin 유발성 당뇨백서의 당대사에 미치는 영향

박상기,문경래,박춘호,김갑승,박영봉,이병래,양남웅 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

Streptozotocin(STZ) may produce a permanent form of insulin-dependent diabetes (IDD) in experimental animals. This STZ-induced diabetes has become an useful model to study the pathogenesis of IDD in man primarily because it approximate clinical findings and also because it lends itself easily to controlled manipulation. The diabetogenic activity of STZ, however, is known to be modified by several agents such as nicotinamide, diazoxide, adrenergic blockers and pertussis vaccine (PV). The present study was undertaken to observe the protective effect of PV or boiled pertussis vaccine(bPV; incubating the PV at 80℃ for 30 minutes) against the development of IDD induced by STZ in young rats, and also the effect of PV on the activities of glycolytic & gluconeogeneic enzymes in both liver and muscle of rat. PV at a dose of 1.2×10^(10) microorganism was administrated intrapntoneally on 3days before & 7days after a single injection of STZ (GO㎎/㎏ body wt). The blood glucose and insulin levels were measured at 1st wk, 2nd wk, 4th wk, 6th wk and 8th wk after STZ injection, and the enzyme activities were exemined at 8th wk. The following results were obtained. 1. No significant difference was noted in blood glucose levels between STZ group and STZ+PV or STZ+bPV groups (P>0.05). 2. Blood insulin levels in STZ group decreased significantly from 2nd wk(P<0.05). Compared with STZ group, blood insulin levels in STZ+PV & STZ+bPV groups were higher, but not a significant value except at 2nd wk in STZ+PV grouP (P<0.05). 3. Compared with STZ group, hepatic glycolytic enzyme activities were significantly increased in STZ+PV or STZ+bPV groups, and muscular glycolytic enzyme activities were significantly increased in STZ+PV or STZ+bPV groups. 4. Compared with STZ group, hepatic gluconeogeneic enzyme activities were significantly increased in STZ+PV or STZ+bPV groups. These results suggest that the PV has somewhat protective effects on blood insulin levels and tissue glycolytic enzyme activities in rats, but it could not reduced blood glucose levels significantly, probably because of excessive increase of hepatic gluconeogeneic enzymes.

Polyester 축중합 공정 회분식 반응기의 전산 모사 및 운전 최적화 연구

박융호,강우철 漢陽大學校 工學技術硏究所 1996 工學技術論文集 Vol.5 No.1

A batch polycondensation reactor for polyester manufacture has been modeled based on plant data using a process simulator (Aspen Plus). Model includes major aspects of polycondensation reactions, side reaction, vapor liquid equilibria, and mass transfer and predicted correctly the dependency of the process performance on the temperature and the pressure of the reaction. Various profiles of the temperature and the pressure were tested for its performance and the profiles which improves significantly the degree of polymerization of the final product were found. The individual and combined effects of the temperature profile and pressure profiles were examined and a way to shorten the batch time using optimized profiles has been discussed.

들깨잎의 품종에 따른 성분분석 및 생리활성물질 탐색

한호석,박정혜,최희진,손준호,김영활,김성,최청 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.1

The biochemical components of Namcheondlggae, Miryangdlkkae 25, Boradlggae and Ipdlkkae 1 were measured. The samples were extracted with hot water, 60% acetone or 80% ethanol for screening physiological activity. The crude protein content (4.36%) was found in the Miryangdlkkae 25 and calcium content (497.5 mg%) was found in the Namcheondlggae among the tested 4 perilla leaves. Fructose was 30.86 mg% in the Namcheondlggae and free amino acids at all perilla leaves was detected seventeen. In Boradlggae, glutamic acid and alanin were 25.37 and 11.91 mg%. Totally nine non-volatile organic acids were also detected and the contents of malic acid and glutaric acid were 28.34 and 14.57 mg% in Boradlggae. The Miryangdlkkae 25 had the highest vitamin C amount which was 113.24 mg%. Angiotensin converting enzyme (ACE) inhibition activity of 80% ethanol extract of Boradlggae was 46.71%. Electron donating activity of 60% acetone extract from Namcheondlggae was the strongest inhibition activity as 98.19% when 200ppm level of the sample extracts were added.

果實·菜蔬類의 窒酸鹽 및 亞窒酸鹽含量

金章亮,朴榮浩,千石祚 釜山水産大學校 1984 釜山水産大學 硏究報告 Vol.24 No.1

果實·菜蔬類의 窒酸鹽 및 亞窒酸鹽의 含量을 밝히기 위하여 배추, 양배추, 엇갈이배추, 상치, 시금치, 가지, 오이, 양파, 당근, 파 및 무 등 11종류의 菜蔬類와 사과(紅玉, 골든, 골든델리셔스, 陸奧), 감(富有, 堂上蜂屋), 포도(Tanored, Seibel 9110)등 10종류의 果實類를 食料로 하여 生體의 各部位別로 나누어 그 含量을 調査, 檢討하였는데, 그 結果는 다음과 같다. 1. 배추, 양배추, 엇갈이배추, 상치 및 시금치등의 菜蔬類에 있어서는 全般的으로 葉柄部의 窒酸鹽含量이 다른 部位에 비하여 높은 傾向을 나타내었으며 특히, 시금치의 葉柄部는 2050ppm으로 가장 높은 含量을 나타내었고, 이어, 엇갈이배추, 상치, 배추, 양배추의 順이었다. 2. 가지 및 오이등의 果菜類에 있어서는 果皮部가 果肉部보다 높은 窒酸鹽含量을 나타내었으마, 그 量은 菜蔬類에 비하여 全般的으로 적어서 오이에서는 108ppm, 가지는 169ppm이였다. 3. 양파, 당근, 대파 및 무 등의 根菜類에 있어서는 일반적으로 根部보다는 葉柄部가 있는 上端部의 窒酸鹽含量이 높은 傾向을 나타내었다. 종류별로는 무의 含量이 가장 높아 1101ppm을 나타내었으며, 이어, 당근 대파, 양파의 順이었는데, 특히, 양파의 含量이 적어 16ppm에 지나지 않았다. 4. 菜蔬類의 亞窒酸鹽含量은 全般的으로 낮아 0.1∼3.0ppm였으며, 菜蔬類에 있어서는 葉柄部의 含量이 높은 경향을 보였으나 果菜類 및 根菜類에 있어서는 部位別에 따른 뚜렷한 차이를 찾아볼 수 없었다. 5. 果實類의 窒酸鹽含量에 있어서는 全般的으로 菜蔬類에 비하여 월등히 낮은 含量을 나타내었으며, 果皮部가 果肉部에 비하여, 높은 경향을 보였다. 試料果實中에서는 사과의 含量이 가장 높았으며, 이어, 배, 포도, 감의 순이었고 사과에 있어서는 紅玉, 골든 및 골든델리셔스등은 31∼32ppm으로 비슷한 含量이었으나, 陸奧는 5ppm정도로 월등히 적은 含量을 나타내었다. 6. 果實類의 亞窒酸鹽含量은 全般的으로 극히 낮은 편이었으며, 특히, 배의 경우는 거의 검출되지 않았다. 試料果實中 감의 含量이 가장 높아 紅枾의 경우에 있어서는 窒酸鹽含量과 거의 비슷한 2.4ppm으로 나타났다. It has been known that nitrates and nitrites accelerate not only the corrosion of tinplate, but also the formation of carcinogen. This study was carried out to elucidate the distribution of nitrates and nitrites from the fruits and vegetables by portions. Nitrate contents in the petioles of vegetables were higher than those of other portions. Especially, nitrate content in the petiole of spinach was the highest as 2050 ppm. There were no appreciable differences in the contents of nitrites of the vegetables by portions. Nitrite contents of the vegetables were less than 1.0 ppm except spinach and chininese cabbage for 2.2-3.0 ppm. Nitrate contents in the fruits were generally low in comp arison with those of vegatables. Nitrate contents of fruit skins were more than those of other portions. On the other hand, notrites were not detected in pears.

活性酸素種에 의한 Melanoidin의 脫色 및 分解生成物 : Ⅰ. 過酸化水素에 의한 脫色 및 分解 Ⅰ. Decolorization and Degradation of Melanoidins by Hydrogen Peroxide

金善奉,朴榮浩 釜山水産大學校 1985 釜山水産大學 硏究報告 Vol.25 No.2

D-glucose-glycine系로 부터 調製한 非透析性 Melanoidin에 過酸化水素를 使用하여 脫色 및 分解를 행하고 그 分解條件의 檢討와 分解生成物의 同定 및 部分構造를 推定하였다. Glucose oxidase를 使用하여 Melanoidin에 作用시켰을 때 Melanoidin의 脫色率은 65%로 나타났다. 本酵素에 의한 Melanoidin의 脫色은 酵素-基質間의 反應生成物인 過酸化水素등에 의한 것으로 밝혀졌다. 그래서, 中性 및 알칼리性 條件下에서 Melanoidin에 過酸化水素를 加하고 37℃에서 28時間 反應시켰을 때, 中性條件下에서의 脫色率은 64%, 알칼리性條件下에서는 97%이었다. 이 處理에 의하여 平均分子量 5,300의 未處理 Melanoidin이 平均分子量 3,500까지 低分子化하였다. 한편, 非透析性 Melanoidin의 過酸化水素處理에 의하여 얻어진 에테르可溶性劃分中의 主要分解生成物은 2-methyl-2, 4-pentanediol, N, N-dimethylacetamide, acetic acid, oxalic acid, Methylpropanedioic acid, propanedioic acid, Butanedioic acid, 2-furancarboxylic acid, 2-hydroxypropanoic acid, 2, 5-furandicarboxylic acid, 5-(hydroxymethyl)-2-furancarboxylic acid 등이었다. 水溶性劃分中의 低分子分解生成物은 glycine이 대부분이었고, 量的으로는 Melanoidin當 1.73%이었다. 이들 Melanoidin의 低分子化 및 分解生成物은 Melanoidin의 糖側鎖-C-CO-結合의 解裂 및 NH-CO結合의 切斷에 의한 것으로 推察된다. Nondialyzable melanoidins prepared from glucose-glycine system were incubated with glucose oxidase under optimal conditions and then the melanoidins were decolorized at a degree of 65%. The decolorization of melanoidin by glucose oxidase mainly caused by hydrogen peroxide produced from enzyme-substrate reaction. Therefore, the oxidative decomposition and decolorization of melanoidins were tried by using hydrogen peroxide. Melanoidins were treated with hydrogen peroxide (final conc. 6.72%) under neutral (pH 7.0) and alkaline (pH 10.0) conditions at 37℃ for 28 hr. Melanoidins were decolorized to 64% and 97% under above optimum conditions, respectively. The mean molecular weight of melanoidins decreased from 5,300 to 3,500 before and after hydrogen peroxide treatment. The major components in ether-soluble fraction obtained from melanoidins by oxidative degradation of alkaline hydrogen peroxide were identified as 2-methy1-2, 4-pentanediol, N, N-dimethylacetamide, phenol, acetic acid, oxalic acid, methylpropanedioic acid, propanedioic acid, 2-furancarboxylic acid, butanedioic acid, 2-hydroxypropanoic acid, 2, 5-furandicarboxylic acid and 5-(hydroxymethyl)-2-furancarboxylic acid. On the other hand, the major degradation product in aqueous fraction was identified as glycine, and 1.73% glycine was produced per melanoidins.