Proceeding of 13th Scientific Meeting ; Antitumor Effect of 1 , 3 - bis ( 2 - Chloroethyl ) - 1 - nitrosourea with tubercin - 3 - on Ehrlich Ascites Carcinoma in Mice

정태호 ( Tai Ho Chung ),김여희 ( Yeu Hee Kim ) 한국생화학분자생물학회 (구 한국생화학회) 1978 BMB Reports Vol.11 No.4

인삼 성분이 당질의 산화 및 생성에 미치는 효능

조준승,정태호,곽춘식,김중영,Jo, Joon-Seung,Chung, Tai-Ho,Kwak, Chun-Sik,Kim, Choong-Young 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.1

인삼 성분을 메탄올로써 추출하고 이것이 쥐에 있어서 당질의 산화와 당의 생성 즉 glycogenesis 및 glyconeogenesis 그리고 당 신생반응의 첫 단계 반응을 조절하는 효소라고 믿고 있는 간의 phosphoenolpyruvate carboxylkinase의 활성에 어떠한 영향을 미칠것인가를 조사하여 보았다. 인삼 추출물을 쥐 체중 100g당 5mg이 되도록 생리적 식염수 0.5ml에 녹여서 1일 1회 4일간 복강내로 미리 투여하였으며 대조군에는 같은 양의 식염수만 주입하였다. 이것을 다시 계속 섭식시킨 군과 24시간 굶긴 군으로 나누어서 인삼 추출물을 투여한 1시간뒤에 glucose-$^{14}C$ 또는 amino acid mixed-$^{14}C$를 주입하여 호기중애 배출되는 $^{14}CO_2$ 양을 검출하고 또 3시간 후에 간속의 glycogen과 phospho-enolpyruvate caboxykinase에 대해서 검토하였다. 이 실험 결과 인삼은 glucose의 산화를 현저히 증가시켰으며 이 효과는 기아상태에서 더욱 촉진적으로 작용하였다. 그러나 인삼은 간에서의 glycogenesis나 glyconeogenesis에 대해서는 효능이 그리 없는것 같았다. 즉 인삼투여군은 대조군에 비하여 간의 glycogen농도나 $^{14}C$ 화합물의 glycogen에의 편입속도가 다같이 별 차이를 나타내지 않았다. 그리고 간의 phosphoenolpyruvate carboxykinase의 활성에도 인삼의 별 영향을 미치지 않았다. Cultivated dry ginseng root was pulverized and extracted with methanol. The extractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200~220g were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5mg per 100g body weight of the ginseng extract in 0.5ml of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 uc of glucose-$^{14}C$ with carrier 100mg glucose per 100g of body weight or $4\;{\mu}c$ of amino acid mixed-$^{14}C$ with carrier 20mg L-alanine per 100g of body weight were injected peritoneally for the correspond group and control. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase, To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately after the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-$^{14}C$ or amino acid mixed-$^{14}C$ in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the adminisration of ginseng both the time of feeding and fasting.

인삼 성분이 당질의 산화 및 생성에 미치는 효능

조준승,정태호,곽춘식,김중영 ( Joon Seung Jo,Tai Ho Chung,Chun Sik Kwak,Choong Young Kim ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1

Cultivated dry ginseng root was pulverized and extracted with methanol. Theextractive was washed with ether three times and filtered. The filtrate was concentrated under reduced pressure and lipophilized to be dried. Sprague-Dawley rats weighing 200∼2208 were divided into the ginseng and the saline administered groups. The ginseng group were received daily 5㎎ per 1008 body weight of the ginseng extract in 0.5㎖ of saline peritoneally for 4 days, and the saline group received the same amount of saline. The ginseng and saline groups were further divided into the fasted group, maintained in fasting state for 24 hours before the last administration of ginseng, and the fed group, which was kept in feeding during the experiment. A hour later the administration of ginseng or saline to the corresponding, 4 μc of glucose-^(14)C with carrier 100㎎ glucose per 1008 of body weight or 4 μc of amino acid mixed-^(14)C with carrier 20㎎ L-alanine per 1008 of body weight were injected peritoneally for the correspond group and control.. Three hours after the administration of glucose or amino acid rats were killed to be determined the content of liver glycogen and phosphoenolpyuvate carboxykinase. To estimate the oxidation rate of glucose, rats were put in a metabolic cage immediately alter the injection of glucose. The expired carbon dioxide was collected into barium hydroxide solution and the radioactivity was counter by gas flow counter. It was revealed that the ginseng root extract increased the oxidation of glucose in rat in the early period of the time after a glucose injection. This effect was more prominent in the fasting state. Ginseng, however, does not so much effect on glycogensis and glyconeogenesis when the animal was at the state of feeding or fasting since glycogen content and the rate of glycogen synthesis from glucose-^(14)C or amino acid mixed-^(14)C in ginseng administered groups showed no fructuation compared with that of the control. Activity of phosphoenolpyruvate carboxykinase considered as a regulatory enzyme for glyconeogenesis was not changed by the administration of ginseng both the time of feeding and fasting.

외과적 중환자에서 혈청중 Tumor Necrosis Factor, Interleukin-1, Interleukin-6 및 Neopterin의 변동

정기용(Kee Yong Chung),장수일(Soo Il Chang),정태호(Tai Ho Chung) 대한외과학회 1994 Annals of Surgical Treatment and Research Vol.46 No.5

Can the Expression of Histocompatibility Antigen be Changed by Lithium?

Byung-Jo Kang(강병조),Sang-Wun Park(박상운),Tai-Ho Chung(정태호) 대한생물치료정신의학회 1997 생물치료정신의학 Vol.3 No.2

This study was attempted to find out whether HLA type changes would be caused by lithium. Fifteen patients were chosen as subjects(4 males, 11 females). Fifteen completed tests in class Ⅰ and 8 in class Ⅱ. Their mean age was 27. For an averaage of 51 days, lithium 600-1200 ㎎/day(mean daily dose: 900㎎) was administered for HLA tests to be compared with the HLA type before the drug was applied. Class Ⅰ type test was done by Terasaki et al's microcytotoxicity method and class Ⅱ type by Erhlich et al's polymerase chain reaction method. The results were: Eleven of the 15 subjects had changes in HLA-A, B, C types. Two of the 8 subjects had changes in HLA-DR type. In conclusion, lithium in the therapeutic dose is considered to bring about changes in HLA expressions in as short as 2 months. 목적: 김문두등(1996)은 haloperidol, lithium 및 amitriptyline이 인체에서 HLA type에 변화를 초래할 수 있다는 보고를 한 바 있다. 그리하여 저자들은 면역계에도 영향을 미치는 백혈구증다증도 가져올 수 있는 lithium이 치료용량으로 인체에서 HLA class Ⅰ형과 Ⅱ형에 어떤 영향을 미치는가를 좀 더 상세히 알아보고자 본 연구를 시도하였다. 대상 및 방법 : DSM-IV에 근거하여 양극성 장애 10명, 분열정동장애 3명, 충동조절장애 1명, 행실장애 1명 총 15명이 연구대상이었다. 이들에게 lithium 투약전과, 일일평균 900㎎의 lithium을 평균 51일간 복용시킨후 각각 말초 혈액을 채취하여 HLA형을 비교하였다. HLA class Ⅰ형은 Terasaki등의 임파구세포상해 시험법, HLA-DR형을 PCR 방법으로 결정하였다. 결과 : class Ⅰ형은 15명중 11명에서 있던 항원형의 소실이나 새로운 항원형의 출현등 변화가 있었다. class Ⅱ형인 DR형에서도 8명중 2명에서 항원형의 변화가 있었다. 결론 : lithium을 치료용량으로 약 2개월정도 투여하여도 HLA의 표현형에 변화를 가져올 수 있다고 보여진다.

Mildronate가 혈청 알코올 농도와 숙취에 미치는 영향

박선민(Sun-Min Park),강박광(Bak-Kwang Kang),정태호(Tai-Ho Chung) 한국식품영양과학회 1998 한국식품영양과학회지 Vol.27 No.1

본 연구는 실험동물인 가토와 음주 경험이 많은 남자 대학생을 대상으로 carnitine의 유사체인 Mildronate 복용이 음주 후 혈중 알코올의 농도와 숙취에 미치는 영향을 조사하였다. 동물실험과 임상실험에서 모두 Mildronate를 복용하였을 때 알코올 분해속도를 촉진시키지 못하였고, 혈중 알코올 농도도 감소시키지 않았다. 임상실험에서 간세포의 파괴 정도를 나타내는 indicator인 혈청내 GOT의 농도는 현저하게 감소하여서 Mildronate가 알코올의 대사산물로 인한 독성을 감소시킨다는 것을 알았다. 또한 숙취에 대한 설문조사에서 Mildronate를 복용하였을 때 술이 깬 후 두통과 속 쓰림이 감소하였는데 이것은 Mildronate가 중추신경계에 작용하여 알코올에 의한 해로운 작용을 완화시켰던 것으로 여겨진다. 즉, Mildronate를 음주 직전에 복용하였을 때 알코올의 분해를 촉진시키지는 않았지만, 알코올로 인한 간세포의 파괴를 감소시켰고, 술깬 후 숙취도 감소시키는 경향을 나타내었다. 그러나 아직까지 그 기전을 설명하기는 어렵고, 이에 대한 연구가 요구된다. 결론적으로 음주 전 Mildronate를 복용하였을 때 알코올의 분해속도를 촉진시키지는 않았지만, 알코올 섭취로 인한 부작용인 숙취 현상과 간세포의 손상을 감소시키는 좋은 효과를 나타내었다. 또한 본 연구는 Mildronate를 음주 전에 복용하였을 때의 효과를 측정하였는데, 음주 후 Mildronate를 복용하였을 때 숙취에 미치는 현상에 대한 연구도 필요하겠다. This study was conducted to determine whether Mildronate, an analogue of carnitine, influences blood alcohol concentration and hang-over syndrome in rabbits and healthy college male students. In the animal study, ten rabbits were randomly divided into two groups. One hundred ㎎ per 1㎏ body weight of Mildronate was injected twice into five rabbits before injecting 50% ethanol; the rest of rabbits were injected with saline. The human study was performed with two sections. Each section of the study was conducted by a two-phase cross-over design with a four day wash-out period. All volunteers took Mildronate in one phase, and took a placebo in the next phase. The difference between the two sections was related to the time of taking the Mildronate pill and the amount of alcohol consumed. Blood alcohol concentrations were not significantly different between in those taking Mildronate and in those taking the placebo in both the human and the animal study. However, the concentration of serum aspartate aminotransferase(AST, GOT), the indicator of liver cell damage, was lowered in those taking Mildronate, compared to those taking the placebo. Also, headache and heartburn among hang-over syndrome patients were less severe with Mildronate. In conclusion, taking Mildronate prior to drinking alcohol can somewhat reduce liver cell damage and hang-over syndrome without stimulating alcohol metabolism.

각종 간질환환자에서 혈청 N - acetyl - β- D - Glucosaminidase 와 Monoamine Oxidase 의 활성도 변화

허정욱(Jung Wook Hur),박승국(Soong Kook Park),정태호(Tai Ho Chung) 대한내과학회 1987 대한내과학회지 Vol.34 No.1

N/A Glycosaminoglycan, a component of connective tissue, increased significantly in tissue fibrosis including liver cirrhosis. It has been reported that N-acetyl-0-D- glucosaminidase takes place important role in tissue fibrosis and that the degree of fibrosis is reflected in serum N-acetyl- β -D-glucosaminidase activity in hepatic fibrosis. Morevoer, in tissue fibrosis collagen formation is the main event. And in Collagen formation monoamine oxidase has function of fixation of the soluble collagen molecule and of stimulation of the connective tissue maturation. It also has been reported that serum monoamine oxidase activity reflects hepatic monoamine oxidase activity and the activity is related to the synthetic activity more than the accumulated amount of the collagen. The authors have investigated the activities of N- acetyl-β-D-glucosaminidase and monomaine oxidase in the serum in 15 healthy subjects and 55 patients with various liver diseases. In patients with chronic hepatitis, liver cirrhosis and hepatoma, serum N-acetyl- β -D-glucosaminidase activity increased twice as the value of healthy subjects. Serum monomaine oxidase increased significantly in chronic hepatitis (p<0.001) and liver cirrhosis (p<0.01). No definite correlation was found between any of liver function tests or the evidence of protal hypertention and these two enzyme activities on patients with liver cirrhosis. These results suggest that serum N-acetyl- β -D- glucosaminidase and monomamine oxidase activities can be used as an important index in the evaluation of the hepatic fibrogenesis and its therapy.

惡性腫瘍患者의 DNCB 皮膚減作과 免疫療法(Tubercin-3 投與)後의 臨床的 觀察

金世民(Sae Min Kim),申澤秀(Taik Soo Shin),金東龍(Dong Yong Kim),鄭泰浩(Tai Ho Chung) 대한외과학회 1978 Annals of Surgical Treatment and Research Vol.20 No.8

각종 간질환에서의 (肝疾患) 혈청 (血淸) High Density Lipoprotein - Cholesterol 의 변동

안성훈(Sung Hoon Ahn),박승국(Seung Kook Park),허정욱(Jung Wook Hur),이호찬(Ho Chan lee),김정철(Jung Chul Kim),정태호(Tai Ho Chung) 대한소화기학회 1984 대한소화기학회지 Vol.16 No.1

N/A In patients with liver disease, abnormalities of cholesterol metabolism has been suspected. Hence concentrations of cholesterol in whole serum, and cholesterol concentrations in the high density lipoprotein fraction of serum was measured after the precipitation of low density and very low density lipoproteins with sodium phosphotungstate-13Mg in l patients with liver cirrhosis, 10 patients with acute hepatitis, 10 patients with chronic hepatitis, 7 patients with hepatoma, 5 patients with obstructive jaundice, and 24 healthy control subjects. Total cholesterol concentrations in patients with hepatic cirrhosis were significantly lower than control, but in patients with chronic hepatitis, and obstructive jaundice, total cholesterol concentrations were slightly higher than control. High density lipoprotein cholesterol concentrations in various liver disease were significantly lower than control, and their ratios of high density lipoprotein cholesterol to total cholesterol were also significantly low.

오리(Duck) B형 바이러스 간염에 대한 생약제 가자(訶子), 지유(地楡), 복분자(覆盆子), 그리고 대황(大黃)의 치료효과

정태호,이종률,김정철,김문규,이인선,장원희,이인수,김영철,정혜리,김승호,함경수 慶北大學校 醫科大學 1993 慶北醫大誌 Vol.34 No.4

사람의 B형 간염을 치료할 목적으로 한국산 생약제 151종으로부터 시험관 내에서 HBsAg과 결합능이 있고 HBV DNA plymerase 활성을 억제하는 가자(訶子), 지유(池楡), 복분자(覆盆子), 그리고 대황(大黃)을 duck hepatitis B virus(DHBV)에 감염된 오리에 투여하여 감염 2주 후와 4주 후의 혈청중 DHBV-DNA와 DHBV-DNA polymerase활성도를 조사하였다. 가자(訶子)를 투여한 오리에서는 4주 후에 DHBV-DNApolymerase 활성도가 생약 투여전에 비하여 유의하게 낮아졌으며 지유(地楡)를 투여한 오리에서는 투여 전에 비하여 유의한 차이를 보이지 않았다. 복분자(覆盆子)와 대황(大黃)을 투여한 군에서는 DHBV-DNA와 DNA polymerase활성이 다같이 유의한 저하를 보여주었다. 기간중에 모든 오리에서 혈청중 transaminase활성과 간장의 병리조직학적 소견은 변동을 보이지 않았다. This study was performed to evaluate the effect of extracts of 4 traditional Korean herbal medicines on hepatitis B virus. 151 traditional herbs were extracted, filtered and lyophilized. Using 151 herbal extracts we tested binding capability to the HBsAg and inhibition of HBV DNA polymerase activity. We had determined that among 151 herbs, 4 herbs (Terminalis chebula, Sanguisorba officinalis, Rubus coreanus, and Rheum palmatum) showed binding capability to HBsAg which contained in the commercial hepatitis vaccine, at 16㎍/㎖ of concentration and also significantly inhibited the human hepatitis B virus DNA polymerase activity. To investigate the in vivo effect of Terminalis chebula, Sanguisorba officinalis, Rubus coreanus, and Rheum palmatum extracts on the sequential changes of duck, each 7 DHBV carrier ducks were treated with Terminalis chebula(50㎎/㎏), Sanguisorba officinalis(50㎎/㎏), Rubus coreanus(50㎎/㎏), and Rheum palmatum(25㎎/㎏) 3 times per week for 4 weeks. Serum samples were obtatined before treatment and 2 and 4 weeks after administration of herbs from each group of ducks to determine the sequential changes of serum DHBV-DNA and DHBV-DNA polymerase activities. DHBV-DNA was analyzed by the method of spot hybridization test and the DHBV-DNA polymerase activity was determined by the method of Laplan et al. Treatment with Terminalis chebula brought sequential decrease of quantities of DHBV-DNA amount was not significant compared with the values of before herb administration. In the Sanguisorba officinalis treated animals, sequential changes of both the calues of DHBV A-DNA and DNA polymerase activities showed irregular fructuation. Two groups of animals treated with Rubus coreanus and Rheum palmatum brought the significant decrease both in the sequential examination of the serum. Terminalis chebula, Rubus coreanus, and Rheum palmatum seemed to have in vivo anti-viral effect on DHBV infected ducks. No significant change of serum transaminase activities were determined in all animals during experimental period, and unremarkable pathological changes were noticed by the microscopic examination in the liver tissues obtained after 4 weeks.