골인이 Morphine 중독 마우스의 금단 증상에 미치는 효과

정태호,김정철,채성철,강병조,이인자 최신의학사 1991 最新醫學 Vol.34 No.2

151종 생약제 추출물이 B형 간염 바이러스 표면 항원(HBsAg)과 항체(HBsAb)의 결합반응 그리고 HBV DNA polymerase 활성에 미치는 효과 : 한국산 각종 생약제를 이용한 B형 간염치료제의 개발

정태호,김정철,김문규,이인선,채성철,김승래,정준모,이인수,김승호,함경수 慶北大學校 醫科大學 1992 慶北醫大誌 Vol.33 No.3

세계인구 43억명 중 B형 간염 바이러스 보균자는 2억 1천 5백만명이며 이중 78%인 1억 6천만명 이상이 아시아 대륙에 집중되어 있다. 한국도 B형 바이러스 간염의 침윤지대이다. 본 연구는 B형 바이러스 보균자 혹은 만성간염을 치료할 수 있는 생약제를 탐색할 목적의 일환으로 한국에서 시판하는 생약제 151종을 구입하여 증류수로 추출하고 여과한 후 냉동건조하여 HBs 항원에 대한 결합능, HBV DNA polymerase억제능, tumor necrosis factor(TNF) 유리에 미치는 영향, 그리고 galactosamine 유발 간염에 대한간장보호 작용을 조사하였다. 151종의 생약제중 0.16㎍/㎖ 농도에서 간염 vaccine 내에 함유된 HBs 항원과 결합하는 생약제는 지유, 가자, 복분자, 대황, 정향, 빈량, 산수유, 소목, 마황, 쇄양, 후박, 목과, 양강, 오약, 산사, 목단피 등 16종이었다. 이들 생약제중 대황(Rheum palmatum L. 大黃), 가자(Terminalia chebula R. 訶子), 지유(Sanguisorba officinalis L. 地楡), 복분자(Rubus coreanus M. 覆盆子)는 HBV DNA polymerase 활성도를 비교적 강하게 억제하였으며 빈량(Areca catechu L. 빈랑), 정향(Eugenia caryophyllata T. 丁香), 목과(Chaenomeles japonica L. 木瓜), 산수유(Cornus officinalis S. 山茱萸)는 약하게나마 HBV DNA polymerase 활성을 억제하였다. 또한 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 대상으로 TNF 유리에 미치는 영향을 조사한 결과 소목을 제외한 8종의 생약제 모두가 대식세포를 자극하여 TNF를 유리시켰다. 그리고 장차 임상에 사용함을 전제로 HBV DNA polymerase를 억제한 대황, 가자, 지유, 복분자, 빈량, 정향, 마황, 소목, 산수유를 galactosamine 유발 간염(백서)에 투여하여 간기능 검사에 미치는 효과를 조사하였으나 간기능 검사상으로는 특별한 보호작용을 나타내지 못하였으며 생약제 단독 투여로서는 간기능에 대하여 나쁜 영향을 주지 않았다. Hepatitis B virus infection is major cause of acute and chrome hepatitis, cirrhosis of liver, and hepatocellular carcinoma. There are over 200 million carriers of hepatitis B virus in the world, nearly 80 % of whom live in the South East Asia, Korea is also highly endemic area of hepatitis B virus infection. This study was performed to evaluate the effect of extracts of traditional Korean herbal medicines on hepatitis B virus. 151 traditional herbs were extracted, filtered and lyophilized. Using 151 herbal extracts we tested binding capability to the HBs Ag, inhibition of HBV DNA polymerase, release of tumor necrosis factor (TNF), and protection against galactosamine induced liver damage. Among 151 herbs, 16 herbs (Sanguisorba officinalis L., Terminalia chebula R., rubus coreanus M., Rheum palmatum L., Eugenia caryophyllata T., Areca catechu L., Cornus officinalis S., Caesalpinia sappan L, Ephedra sinica S., Cynomorium songaricum R., Machilus thunbergii S., Chaenomeles japonica L., Alpinia officinarum H., Lindera strychnifolia V., Crataegus pinnatifida B., Paeonia suffruticasa A. ) had binding capability to HBs Ag which contained in the commercial hepatitis vaccine, at 16 ug/㎖ of concentratioa Among the above 16 herbs, Rheum palmatum L., Terminalia chebula R., Sanguisorba officinalis L., Rubus coreanus M. significantly inhibited the HBV DNA polymerase activity, and Areca catechu L., Eugenia caryophyllata T., Ephedra sinica S., Caesalpinia sappan L., Chaenomeles Japonica L., Cornus officinalis S. also inibited the HBV DNA polymerase activity but less effective. All of the herbs, except Caesalpinia sappan L., which inhibited the HBV DNA polymerase activity, actively stimulated the mononuclear cells to release tumor necrosis factor(TNF) in whole blood culture system. The herbs which inhibited the HBV DNA polymerase activity, did not show any protective effect in galactosamine-induced hepatitis in rats, but there was no direct toxic effect when herbs were administered alone.

Sarcoma 180 誘發 생쥐의 生存延長에 對한 Cyclophosphamide, Picibanil, 및 Tubercin-3의 單獨 및 倂合投與의 有效成

鄭泰浩,孫健英 慶北大學校 醫科大學 1981 慶北醫大誌 Vol.22 No.1

생쥐에 Sarcoma 180을 유발 시키고 각각 그 작용점이 상이한 면역요법제 Tubercin-3와 Picibanil의 단독 요법과 병합요법 그리고 화학요법제 Cyclophosphamide와의 병합사용의 효과를 검토 하였다. Sarcoma 180 유발후의 생쥐의 생존일수의 평균치는 대조군 11.1일 : Tubercin-3투여군 14.8일 : Picibanil 투여군 12.4일 : 그리고 Tubercin-3과 Picibanil의 병합투여군은 8.4일 이었다. Cyclophosphamide 단독투여군의 생존일수는 18.1일이었고 Cyclophosphamide와 Tubercin-3 병합군은 27.4일, Cyclophosphamide와 Picibanil 병합군 22.6일로서 상당히 연장 되었으나 Cyclophosphamide와 두가지 면역요법제를 같이 사용한 동물군의 생존일은 평균 18.7일로서 면역요법제를 사용하지 않은 군과 같았다. This study was carried out to determine the efficacy of combined treatment of cyclophosphamide with Tubercin-3 and Picibanil. One hundred sixty sarcoma 180 bearing mice were divided eight groups of 20 animals each group receiving saline, Tubercin-3, Picibanil, and Cyclophosphamide alone and Cyclophosphamide combined with Tubercin-3, with Picibanil and with both Tubercin-3 and Picibanil respectively. The treatment was initiated 48 horus after tumor cell implantation and repeated three times given once a day with following doses suspended or dissolved in 0.2㎖ of saline at the interval of every other days: 0.5 microgram Tubercin-3 0.1㎎ Picibanil; 3.O㎎ Cyclophophamide; either in alone or in combination. Immunotherapeutic agents were administered by subcutaneously and Cyclophosphamide was given abdominally. The observation of general conditions of animal took place twice a day following treatment and the time of death after tumor implantation was determined. Average survival days in each group of animals were as follows: Control, 11.1 days; Tubercin-3, 14.8 days; Picibanil, 12.4 days; Cyclophosphamide 18.1 days. In combined therapy Cyclophosphamide with Tubercin-3, 27.4 days; Cyclophosphamide with Picibanil, 22.6 day and Cyclophosphamide with both Tubercin-3 and Picibanil 18.7 days. Conclusively, the therapeutic potentiation was reflected to be extended survival in combined treatment of a chemotherapeutics Cyclophosphamide with either one of immunotherapeutics. Tubercin-3 or Picibanil. Combination of Tubercin-3 and Picibanil showed to be antagonistic each other.

B형 간염바이러스의 태아와 성인간세포에 대한 감염성 해명 연구

정태호,김성국,서화정,윤영국,임종완,차상호 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1

사람의 태아와 성인의 간세포에 대한 B형 간염바이러스의 감염도를 비교 분석함으로써 HBV 와 간세포간의 친화도내지는 HBV 수용체에 관한 정보를 얻고자 함 대상 및 방법 : 성인이 외상을 입어 부득이 손상된 간조직을 절제하였을 경우와 모자보건법상 임신지속이 어려운 상태에서 부인과적 수술시 21주 전후의 태아 간조직을 얻어 DMEM 을 배지로 50% 용량으로 HBsAg 양성인 사람 혈청을 가하여 5일간 체외 배양하고 병리조직학적 처리후 효소 면역학적 방법으로 HBsAg 를 염색하여 바이러스의 존재 여부를 검색하였다. 결과 : 태아와 성인 간조직을 5일간 체외 배양하였을 때 그 조직상은 간조직임을 인지할 수 있었다. HBV 의 감염여부는 DAKO 사의 효소면역 법으로 조사한 결과 태아의 간조직에서는 HBsAg 양성부위를 발견하지 못하였으나, 성인 간조직에서는 HBsAg 양성부위가 발견되었다. 결론 : 태생 21주 전후의 태아의 간세포는 HBV 와 친화도는 아주 미약하거나 없고, 성인 간세포는 HBV에 대한 친화도가 높다. 따라서 태생 21주의 간세포에는 HBV 의 감염이 어렵다는 것을 인지하게 되었다. Considerable progress has been made in the immune prophylaxis of hepatitis B virus(HBV) infection and in the understanding of its replication and pathogenecity at the molecular level. However, a convenient in vitro assay for HBV infectivity has been lacking and the early steps of viral life cycle are now yet well understood. Primary cultured human hepatocytes are reported to be susceptible to HBV 10-12, but these systems are not practical because they are short-lived and require primary explanted human liver. However, it is uncertain yet whether primary hepatocytes of human fetal liver are susceptible to HBV for infection or not. We have expanded the infectivity of HBV into a primary cultures of human fetal hepatocytes together with controversy of adult primary hepatocytes. To investigate the infectivity of HBV for, adult and fetal hepatocytes After 5 day culturing fetal(1) and adult(2) liver tissues, which obtained from a surgical operation on DMEM culture media with half HBV positive serum, separating liver cells and fixing them, the existence of HBsAg antigen was investigated through the immuno-enzymatic staining. HBsAg stained area was not found in the 21 weeks gestation liver cells until 5 days of invitro-culture, while it was found in the adult liver cells. This test result showed that the HBV infection is very hard to happen in the liver cells 21 weeks gestation or prior one.

Alcoholics 환자와 Alcohol Dehydrogenase

정태호,신창규,이종학,강병조 경북대학교 병원 1998 경북대학교병원의학연구소논문집 Vol.2 No.1

저자들은 만성알콜중독의 원인으로 음주자의 성격, 사회요인 등 여러가지 문제들이 게재되어 있지만 유전적 요인도 중요하리라고 생각되어 만성알콜중독자로 진단되어 국립부곡정신병원에 입원가료 중인 환자 30명을 대상으로 ADH2의 다형현상(polymorphism)을 조사하여 알콜중독자가 아닌 건강한 한국인의 유전자형과 비교한 결과 다음과 같은 결과를 얻었다. 만성알콜중독자 30명 중 ADH2-1/ADH2-1 형을 갖는 사람이 27명이었으며 ADH2-1/ADH2-2를 갖는 사람이 3명이었다. ADH2¹의 gene frequencies는 0.68이었으며 ADH²의 gene frequencies는 0.32로서 정상 한국인과 비교하여 유의한 차이가 없었다. This study was performed to know the gene polymorphism of alcoholic dehydrogenase(ADH)-2 in 30 Korean alcohol addicted patients who were admitted at Bugok National Mental Hospital. The results were as follows ADH-2/ADH2-1 type among 30 alcohol addicts were 27 and ADH2-1/ADH2-2 type were 3. Gene frequencies of ADH2-1 were 0.68 and gene frequencies of ADH2-2 were 0.32 These results were not different from the gene polymorphism of ADH2 in non-alcoholic Korean healthy adults.

주요 우울증을 동반한 신경성 식욕과항진증 1례

신석호,권용실,김보연,이정태,이성필 한국의학사 1992 診斷과治療 Vol.12 No.7

經口的 Steroid 性 避姙劑의 長期服用이 韓國女性의 血淸中 Lipoprotein 과 High Density Lipoprotein Cholesterol 含量에 미치는 效果

安東皓,郭春植,鄭春浩 慶北大學校 醫科大學 1981 慶北醫大誌 Vol.22 No.1

한국 경산군에 거주하는 여성 중 경구 피임제를 복용한 78명을 복용 기간에 따라 4개군으로 나누어 혈청중 very low density lipoprotein, 및 low density lipoprotein과 high density lipoprotein cholresterol함량을 조사하여 같은 지역에 거주하는 피임제 복용하지 않은 여성 19명의 lipoprotein, 분획high 및 density lipoprotein cholesterol량과 total cholresterol량을 비교하였다. 피임제 norinyl-1의 사용 후 12∼24개월군, 25∼60개월군, 61∼120개월군, 121∼180개월군의 각군과 대조군 사이에 lipoprotein 및 high density lipoprotein cholresterol과 total cholresterol 함량차는 유의하지 않았으며 각 군간 사이에도 유의한 차는 발견되지 않았다. It is well known that the occurrances of atherosclerosis among Koreans are far less frequent than in the Europeans or Americans. After a prolonged research on the concentration of cholesterol in blood and occurrances and sites of atherosclerosis among the Japanese, the team from Cornell University found that the disease starts among the Japanese later in age than their counterparts in Europe and that the initial site is the brain arteries rather than the coronary. Clinical observations also indicated that the disease started in the brain artery among Koreans, too, which might be a common phenomenon of the Orientals. It is also generally known that the onset of the disease in female immediately after menopause owing to estrogen decrease in blood. The use of steroid contraceptives in women might affect both metabolism of estrogen and lipid fractions in the blood. In the past, partial research was conducted on lipids fractions among the women on oral contraceptives. But few of such studies were made among Korean women who are under different factors of diet, climate, and genes. This study is to determine the lipid fractions in the blood of Korean women who have been on prolonged usage of the contraceptives and to detect the early findings of any changes in lipids. 78 women on oral contraceptives and 19 normal subjects matched for parity entered this study. 78 subject were divided into 4 groups according to their duration of oral pill application. Blood samples were collected at Kyungsan Health Center in the morning before breakfast. Serum lipoproteins were determined by the method of Sakurabayashi and Kawai, total cholesterol by the method of Levinson, and high density lipoprotein cholesterol by the method of Lopes-Virella. Values of serum very low density lipoprotein, low density lipoprotein, total cholesterol, and high density lipoprotein cholesterol were respectively 104.9±5.3mg/dl, 216.6±10.1mg/dl, 153.3±4.4mg/dl, and 54.1±2.Omg/dl, in normal subjects. Mean VLDL, LDL, total cholesterol, and HDL-cholesterol in women receiving oral contraceptives for 12-24, 25-60, 61-120, and 121-180 months were respectively as follows; VLDL, 99.1±7.5mg/dl, 104.0±12.3mg/d1, 95.7±7.1mg/dl, and 109.0±18.9mg/dl, LDL; 235.5±15.7mg/dl, 244.0±18.1mg/dl, 242.2±14.9mg/dl, and 210.1±19.Omg/dl, total cholesterol; 162.2±5.Omg/dl, 157.4±7.5mg/dl, 157.4±6.1mg/dl, and 165.5±9.5mg/dl, HDL-cholesterol; 52.8±1.8mg/dl, 50.4±2.3mg/dl, 56.0±2.3mg/dl, and 55.0±2.5mg/dl, No significant differences were observed between women on oral contraceptives and control.

정신과 병동에서의 억제대 시행에 관한 고찰

이정태,최수호 大韓神經精神醫學會 1982 신경정신의학 Vol.21 No.2

This study deals with some aspects of psychiatric use of physical restraints. A total of 476 patients were admitted to the department of neuropsychiatry of Yoido St. Mary's Hospital, Catholic Medical College from Aug. 1, 1980 to July 31, 1981. Among these patients 73 cases were physically restrained during this period and all included in this study. The results were as follows; 1. 35 patients were males and 38, females. More than one half of the restrained patients were in their twenties (37.0%) and thirties (23.3%). 2. Diagnostically schizophrenia was most frequent (53.5%), and the next was organic brain syndrome (12.4%), and alcoholism (11.0%) in that order. 3. The frequencies of physical restraints per patient ranged from 1 to 9 (total 151 times), and 33 patients were restrained only once. 4. Physical restraint was required on the first day of admission in 21.9% of all cases, but in 39.7% of total number of uses, it was required after 7 days. Most restraints were able to be removed within 24hours. 5. Common impulsive ward behaviors of the physically restrained patients were disruption of therapeutic environment (40 times), assaultive to staffs or patients (35 times) and verbally abusive and combative with staffs (33 times). The most common cause for physical restraints was that for the administration of medical care to non-cooperative patients (70 cases, 46.3%). Violent behaviors requiring a physical restraint were more common in nonschizophrenics than schizophrenics.

전국 응급의학과 수련병원의 응급실 병력지에 대한 분석

임태호,임훈,이종호,강형구,장문준,조광현,장석준,김승호,정상원 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

Background: This study was designed to analyze the current emergency department(ED) medical records of teaching hospitals in Korea. Methods: The five-item questionnaires were mailed to the EDs of 40 hospitals. Among them, 27 questionnaires and 35 ED medical records were returned for reply rates of 67.5% and 87.5%, respectively. Results: 1) The actual number of data elements in the ED medical records used by each hospital varies widely. It ranges from 1 to 15 data elements with an average of 7.5 data elements. 2) Thirteen data elements, signature of nurse, checklist style in review of systems, checklist style in physical examination, neurologic examination, figure of face, Glasgow coma scale, trauma scale, treatment plan, mode of transfer, condition on transfer, documents sent with patient, condition on discharge or discharge instruction, use of pediatric chart and vaccination history are used by less than 50% of the medical records examined. 3) There was no difference in the total number of data elements or in redesign and computerization of ED medical record based on the location of the hospital, the type of hospital administration, or the number of years since the start of EM residency program. 4) There was a statistically increased number of data elements in redesigned medical records. 5) In the survey, 89% of the residents replied that medical records needed to be redesigned. With respect to uniformity, 58% of the residents disagreed. A well-designed checklist chart rather than a descriptive chart was preferred by 89% of the residents. Conclusion: The currently used ED medical records have much room for improvement. The age of the ED had little impact on the quality of ED medical records. More attention and effort in this field are needed. In addition, The Korean Society of Emergency Medicine should provide guidelines for ED medical records.

Benzene과 n-Heptane 混合物의 液膜分離에 있어서 界面活性濟의 影響

金泰澔,鄭泰秀 成均館大學校 科學技術硏究所 1993 論文集 Vol.44 No.2

In the separation of benzene-n-heptane mixture by emulsion liquid membrane in a batch system, the effect of surfactants on the separation factor and stability of liquid membrane was investigated over the surfactant concentration ranging from 0.1 to 1.5wt% at the emulsification time of 5 and 10 minutes, the contact time of 5 and 10 minutes and the settling time of 5 and 10 minutes. The surfactants used were triethanolamine lauryl sulfate, sodium laurylether sulfate and sodium lauryl sulfate. In the concentration range used sodium laurylether sulfate showed better separation than the other two surfactants and the separation factor reached its maximum at the surfactant concentration of 0.5wt% for all three surfactants except several experimental conditions only for sodium laurylether sulfate. In the range of contact time used the separation factor increased as the contact time increased for all three surfactants, however, in the case of sodium laurylether sulfate the separation factor decreased after the contact time of 20 minutes.