Catechol-O-Methyltransferase Activity from Regenerating Liver after Partial Hepatectomy in Rats

곽춘식,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The change of catechol-O-methyltransferase (COMT) activity during regeneration of rat liver was studied. Cytosolic, mitochondrial and microsomal COMTs activities were estimated in regenerating rat livers over a period of ten days after 70% (median and left lateral lobes) partial hepatectomy. The values of Km and Vmax in the hepatic enzymes were also measured. The activities of cytosolic and microsomal COMTs in regenerating rat liver after partial hepatectomy were found to be significantly increased between the second and the third day. Whereas the mitochondrial COMT activity did not change. The Vmax values of the cytosolic and microsomal COMTs in the regenerating rat liver were significantly increased at the second day after partial hepatectomy, however, the Km values of the above hepatic enzymes did not vary in all the experimental groups. Therefore, the results suggest that the biosynthesis of COMT was increased during the regeneration of rat liver.

주정 중독 흰쥐에서 총담관 결찰이 혈청 및 간의 α-D-및 β-D-Glucosidase 활성에 미치는 영향

곽춘식,김여희,임종술 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.2

이 연구는 간담도 질환시 음주의 해로움에 대한 생화학적 배경의 일단을 밝히고자 시행한 실험으로서 급성 및 만성 주정 중독을 시킨 흰쥐에게 총담관결찰로 담즙울체를 야기시켜 혈청과 간의 α-D-glucosidase와 β-D-glucosidase의 활성도를 측정한 것이다. 쥐간의 lysosomal α-D-glucosidase 활성도는 총담관만 결찰한 군보다 만성 주정 중독 후 총담관을 결찰한 군이 총담관 결찰 후 7일부터 14일까지 덜 현저한 증가를 나타내었다. 총담관을 결찰한 군과 만성 주정 중독 후 총담관을 결찰한 군에서 간의 lysosomal acid β-D-glucosidase 및 cytosolic broad specificity β-D-glucosidase 활성도를 비교했을 때는 양군간에 별 차이가 없었다. 총담관을 결찰하고 14일 경과한 군과 총담관 결찰 14일 후 급성 주정 중독을 시킨 군에서 간의 lysosomal α-D-glucosidase, lysosomal acid β-D-glucosidase 및 cytosolic broad specificity β-D-glucosidase의 활성도를 비교했을 때는 양군간에 별 차이가 없었다. 쥐 혈청의 lysosomal α-D-glucosidase와 broad specificity β-D-glucosidase 활성도를 총담관을 결찰한 군과 만성 주정 중독 후 총담관을 결찰한 군을 비교했을 때는 만성 주정 중독 후 총담관을 결찰한 군이 총담관만 결찰한 군보다 더 현저한 증가를 나타내었다. 그러나 총담관을 결찰하고 14일 경과한 군과 총담관 결찰 후 급성 주정 중독을 시킨 군에서 혈청의 이들 효소의 활성도를 비교했을 때는 broad specificity β-D-glucosidase만 총담관 결찰 후 14일 경과한 군보다 총담관결찰 14일 후 급성 주정 중독을 시킨 군이 그 활성도가 더 높았다. 이들 성적 중에서 혈청의 lysosomal α-D-glucosidase와 broad specificity β -D-glucosidase의 활성도가 총담관만 결찰했을 때 보다 만성 주정 중독 후 총담관을 결찰했을 때 더 증가되고 아울러 혈청의 broad specificity β-D-glucosidase 활성도가 총담관 결찰 14일 후 급성 주정 중독을 시켰을 때는 총담관만 결찰한 후 14일 경과했을 때보다 그 활성도가 높은 것은 만성 주정 중독 및 급성 주정 중독시 담즙울체가 야기되면 담즙울체만 있을 때보다 간손상이 증폭된다는 것을 나타낸 결과라 생각되며 이성적은 간손상의 증폭으로 간에서 이들 효소의 누출일 증가된 것이라 할 수 있다. 따라서 이 성적은 담즙울체로 간손상이 있을 때는 음주를 해서는 안된다는 것을 뒷받침하는 자료라 하겠다. The activities of the liver and serum α-D and β-D-glucosidase were studied for cholestasis induced by common bile duct ligation and chronic ethanol intoxication developed, or cholestasis after acute ethanol intoxication for manifestation of the biochemical background of alcohol intoxication in hepatobiliary disease. The groups that received common bile duct(CBD) ligation after being chronically intoxicated with ethanol showed a marked increase at the 7th and 14th day after the ligation in the liver lysosomal α-D-glucosidase activities. However, the activities showd a lower degree than groups of the CBD lgation. The groups that received CBD ligation after being chronically intoxicated with ethanol showed a slight increase after the ligation in the liver lysosomal acid β-D-glucosidase and cytosolic broad specificity β-D-glucosidase activities, and the same was seen in the groups of the CBD ligation. At the 1.5th and 24th hours after the acute intoxication with ethanol which was done after 14 days of the CBD ligation, the rats showed a considerable increase in the liver lysosomal α-D-glucosidase, lysosomal acid β-D-glucosidase and cytosolic broad speciticity β-D-glucosidase activities, and the same was seen in the group sacrificed on the 14th day after the CBD ligation. The groups that received CBD ligation after being chronically intoxicated with ethanol showed a marked increase after the ligation in the serum lysosomal α-D-glucosidase and broad specificity β-D-glucosidase activities. However, the activities showed a far higher degree than groups of the CBD ligation. For the groups of acute intoxication with ethanol done after 14 days of the CBD ligation, the serum broad specificity β-D-glucosidase activities increased markadly, but the activities showed a higher degree than the group with the 14th day after the CBD ligation. In summary, especially, when the acute and chronic ethanol intoxication with cholestasis occurred, the serum lysosomal α-D-glucosidase and broad specificity β-D-glucosidase are higher than in cholestasis because of increased liver cell damage, which causes the enzyme to leak into the blood in great quantity. Accordingly, these results will be the data supporting the alcoholic drink is enzymologically harmful in hepatobiliary disease.

Effect of Melatonin on Hemolysis Induced by Cyclosporine

곽춘식,문교철,서성일 대한신장학회 2001 대한신장학회잡지 Vol.20 No.5

Thiol Methyltransferase Activity in Cholestatic Rat Liver Induced by Commom Bile Duct Ligation

곽춘식,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

Changes of thiol methyltransferase (TMT) activity in cholestatic rat liver were studied. Hepatic subcellular and serum TMT activities were determined in cholestatic rat induced by common bile duct (CBD) ligation over a period 28 days. The mitochondrial and microsomal TMT activities in cholestatic rat liver were found to be significantly increased between the 1st and the 28th day after CBD ligation. The TMT activity in serum was significantly increased throughout the experiments. The Vmax values of the above hepatic TMT in cholestatic rat were significantly increased at the 7th day after CBD ligation. However, the Km values of the above hepatic enzymes did not vary in all the experimental groups. Therefore, the results indicate that the biosynthesis of TMT was increased in cholestatic rat liver. The elevated serum TMT activity is most likely caused by increased hepatocytes membrane permeability due to cholestasis mediated liver cell necrosis.

주정 중독 흰쥐에서 총담관 결찰이 간 및 혈청의 Glyoxalase I 활성에 미치는 영향

곽춘식,김여희,변용준 啓明大學校 醫科大學 1995 계명의대학술지 Vol.14 No.4

The activities of the rat liver and serum glyoxalase-I(GLO-I) were studied in cholestasis induced by common bile duct(CBD) ligation after chronic ethanol intoxication, and in cholestasis before acute ethanol intoxication to establish the biochemical background of alcohol hazards in hepatobiliary disease. GLO-I activities in the rat's liver and serum showed less increases when CBD was ligated after chronic ethanol intoxication than that in the CBD ligation alone. GLO-I activities in the rat's liver and serum showed no more increases when acute ethanol intoxication was induced after CBD ligation than that in the CBD ligation alone. On the other hand, when CBD was ligated after chronic ethanol intoxication, the value of Vmax of the liver GLO-I decreased significantly than that in the CBD was ligated alone. However, the values of Km of the liver GLO-I did not changes in the all experimental groups. Viewed from above results, when chronic ethanol intoxication is combined with cholestasis, GLO-I in the liver seems to be decrease its activity than that in cholestasis, and the cause of the decrease is thought to be decreased biosynthesis.

Effect of Acute Ethanol Intoxication on Hepatic Rhodanese Activity in Rats with Extrahepatic Cholestasis

곽춘식,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.2

Liver and serum rhodanese activities were determined in acute ethanol intoxicated rats with extrahepatic cholestasis induced by common bile duct ligation (CBD) to manifest the biochemical background of alcohol drinking hazard under the hepatobiliary disease. Liver cytosolic and microsomal rhodanese activities and these Vmax values in CBD ligated rats with acute ethanol intoxication were found to be decreased much more than that in CBD ligation alone. However, the difference of Km value on above hepatic enzyme was not found between the experimental groups. On the other hand, serum rhodanese activity in CBD ligated rats with acute ethanol intoxication was greater increased more than that in CBD ligation alone. These results indicate that the biosynthesis of the hepatic rhodanese decreases and the serum rhodanese activity increases in cholestasis combined with acute ethanol intoxication. reflecting damage of aggravated hapatocytic membrane. Accordingly, the resulting data supported the fact that alcoholic drinks were enzymologically harmful to the hepatobiliary disease.

흰쥐 재생간에서의 Aryl Sulfotransferase의 활성도

곽춘식,김여희,신미정 啓明大學校 醫科大學 1995 계명의대학술지 Vol.14 No.4

This study was made to know the changes of aryl sulfotransferase activity in regenerating rat liver. Cytosolic, mitochondrial and microsomal aryl sulfotransferase isozymes I,II and III, IV activities were determined in regenerating liver tissue following 70% (median and left lateral lobes) partial hepatectomy in rats for a period of ten days. The activities of these enzymes in serum were measured, the values of the Km and Vmax in these hepatic enzymes were also measured. The activities of cytosolic, Mitochondrial, and microsomal aryl sulfotransferase I,II in regenerating rat liver tissue showed a significant increase between the second and the third day, the first and the third day, and the twelfth hour and the third day, respectively, following partial hepatectomy. The activities of aryl sulfotransferase isozymes I,II and III,IV in rat serum showed a significant increase between the twelfth hour and the first day following partial hepatectomy. The values of Vmax of cytosolic, mitochndrial and icrosomal aryl sulfotransferase isozyme I,II and III,IV in the regenerating liver showed significant increases at the second day following partial hepatectomy However, the values of Km of the these hepatic enzymes did not change. In View of the above results, aryl sulfotransferase isozymes in regenerating rat liver suggest that these are the enzymes increasing their bisoyntheses in the regenerating stage.

Effects of Intravenous Administration of Taurocholate on Hepatic Aryl Sulfotransferase Activity in Cholestatic Rats

곽춘식,--,-- 대한의생명과학회 2005 Biomedical Science Letters Vol.11 No.1

The possible mechanisms of increased aryl sulfotransferase (AST) isozymes activities in cholestatic rat liver were studied. Hepatic AST-Ⅰ, Ⅱ and -Ⅲ, Ⅳ activities were determined from the experimental rats with common bile duct ligation (CBDL). The Michaelis-Menten constants in these hepatic enzymes were also measured. The activities of mitochondrial AST-Ⅰ, Ⅱ and -Ⅲ, Ⅳ, and microsomal AST-Ⅲ, Ⅳ as well as their Vmax values were found to be increased significantly in CBDL plus taurocholic acid (TCA) injected group than in the control group, such as CBDL alone groups. However, their Km values in the experimental groups did not vary. The results suggest that TCA stimulates biosynthesis of the AST in the liver.

Actinomycin D 의 투여가 총담관을 결찰한 흰쥐의 혈청지질분획의 농도에 미치는 영향

곽춘식 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.2

담즙울체시의 혈청지질들의 농도의 변동과 이들 변동의 기전을 규명하기 위하여 흰쥐의 총담관을 결찰하고 경시적으로 혈청의 중성지방, 총인지질, 총 cholesterol 및 유리지방산의 농도를 측정하는 한편 이들에 대한 acitnomycin D의 영향을 관찰하여 그 성적을 비교검토 하였다. 혈청의 중성 지방, 총 인지질 및 총cholesterol은 총담관 결찰후 현저히 증가되었다. 그러나 혈청의 유리지방산은 의의있는 변동을 보이지 않았다. 한편 이들 지질농도의 증가는 acitnomycin D를 담도 결찰수술전후 투여함으로써 그 농도가 현저히 저하되었는데 그저하의 정도는 acitnomycin D를 투여하지 않은 담도결찰군에 비해 혈청 중성지방은 약 20%, 총 인지질은 약 41% 그리고 혈청 총 cholesterol은 약 26%였다. 그러나 혈청유리지방산은 acitnomycin D를 투여해도 의의있는 변동을 보이지 않았다. The intension of this study was to observe the changes in serum triacylglycerol, total phospholipids, total cholesterol and non esterified fatty acid concentations after the ligation of common bile doct in rats of actinomycin D administration in rats following the above procedure were also studied and observed for comparison. Common bile duct ligation in the rats brought about a rapid increase in serum total cholesterol and triacylglycerol concentration and even a greater increase in serum total phospholipids. But serum non esterified fatty acid remained within the limits of control. Serum total phospholipids showed an increase of 3-and 2-fold at 3rd and 6th day after bile duct ligation respectively. But the increase was markedly inhibited by the administration of actinomycin D (50㎍/100g of body weight) one hour prior to and eighteen hours after the ligation of the common bile duct in rats. Simultaneously, the increased level of both serum total cholesterol and triacylglycerol were also reduced significantly with actinomycin D treatment.

Effects of Intravenous Administration of Taurocholic Acid on Liver Lysosomal α-D- and β-D-Mannosidase Activities in Rats with Extrahepatic Cholestasis

Park, So-Kyung,Kim, You-Hee,곽춘식 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.2

The effects of intravenously administered of high concentration of taurocholic acid (TCA) on α-D- and β-D-mannosidase activities in rat liver lysosomes were studied. These liver lysosomal enzymes, and serum lysosomal α-D- and β-D-mannosidase isozymes activities were determined in experimental rats with common bile duct ligation (CBDL). The liver lysosomal β-D-mannosidase activity as well as the serum lysosomal α-D- and β-D-mannosidase isozymes activities were found to be significantly increased in the CBDL plus TCA injected group than in the control group such as CBDL alone group. However, the liver lysosomal α-D-mannosidase activity was found to be significantly decreased in the CBDL plus TCA injected group. The above results suggest that TCA repress the biosynthesis of the lysosomal α-D-mannosidase and induce the biosynthesis of the lysosomal β-D-mannosidase in the liver. And that the elevated serum lysosomal α-D and β-D-mannosidase isozymes activities are most likely due to increased hepatocyte membrane permeability caused by TCA mediated liver cell necrosis