Metallo-β-lactamase 생성 Pseudomonas aeruginosa 및 포도당 비발효그람음성간균의 검출방법에 관한 연구

신경섭,김원식,손재철,홍승복,최재운,형성민 충북대학교 의과대학 충북대학교 의학연구소 2003 忠北醫大學術誌 Vol.13 No.2

연구목적: Metallo-β-lactamse (MBL)는 carbepenem를 포함하는 대부분의 β-lactam 항균제를 가수분해할 수 있으므로 MBL 생성균의 확산은 이들 균에 의한 감염의 치료에 큰 문제를 야기할 수 있다. 따라서 MBL 생성균에 의한 감염의 확산을 막기 위해 이들 균의 조기 검출이 필요하다. 저자들은 MBL 생성균을 검출할 수 있는 세 가지 방법을 PCR 방법과 비교하여 보았다. 재료 및 방법: Imipenem의 MIC가 8 μg/mL 이상인 50개의 포도당 비발효 그람음성 간균을 대상으로 EDTA-double disk synergy test (EDTA-DDS), Etest MBL, Hodge 변법을 시행하였으며 대조 검사로 IMP-1, VIM-1, VIM-2에 대한 PCR을 시행하였다. 결과: 총 50 균주 중 MBL 생성균은 Pseudomonas aeruginosa 1 균주, Alcaligenens xylosxidans 7 균주 등 8 균주(16%)가 검출되었다. 이들 균주는 모두 VIM-2 형이었으며, EDTA-DDS, Etest MBL은 PCR 방법과 100% 일치하였다. Hodge 변법은 8 MBL 생성균주 중 2 균주를 검출하지 못했다. 결론: 50 균주 중 8 균주가 MBL 생성균이었고 모두 VIM-2 형이었다. Etest MBL은 가격이 비싸 검사실에서는 EDTA-DDS가 적당할 것으로 사료된다. Purpose: Because metallo-β-lactamase (MBL) has activity to hydrolysls against most β-lactam drugs, dissemination of MBL producing bacteria may cause problems of treatment of infection by their microorganisms. Early detection or screening for MBLs will contribute to prevent further spread of resistance. Authors compared three MBL detection methods with PCR for MBLs. Materials and Methods: For 50 isolates of g1ucose nonfermentative gram negative bacilli with reduced susceptability against imipenem (MIC≥8 μg/mL), EDTA-double disk synergy (EDTA-DDS), Etest MBL and modified Hodge test were compared with PCR detection method for MBLs. Results: Among 50 isolates of glucose nonfermentative microorganisms, eight MBL producers including a Pseudomonas aeruginosa and 7 Alcaligenes xylosoxidans were detected. Those isolates were VIM-2 producer. The results of EDTA-DDS and Etest MBL completely agreed with PCR for MBL detection. Two isolates among eight MBL Producers were not detected in modified Hodge test. Conclusion: All of MBL producing bacteria were VIM-2 genotype. In conclusion, EDTA-DDS may be useful method for MBL detection in clinical laboratory due to high cost of Etest MBL strip.

IMT-2000용 수신단 One-chip MMIC의 설계 및 제작

신상문,권태운,최재하 울산대학교 2002 공학연구논문집 Vol.33 No.1

본 논문은 IMT-2000용 수신단 One-chip MMIC의 설계 및 제작에 관한 것이다. LNA, 이중 평형 믹서, LO 발룬, RF 발룬, 그리고 IF 증폭기를 하나의 칩에 집적시켰다. 새롭게 제안된 바이어스 회로는 공정상의 변화와 온도의 변화 등의 원인에 의한 문턱전압의 변화를 보상할 수 있다. 제작된 칩의 측정 결과는 모의실험으로 얻은 결과와 일치하지 않는다. LNA는 변환이득이 평탄하게 동작하지 않을 뿐만 아니라 발진현상도 나타났다. 문제에 대한 분석을 통해 원인을 찾았으며, 이 결과는 향후 연구에 사용하려고 한다. 믹서의 변환손실은 -14㏈, IP3는 4㏈m, 포트간 격리도는 25㏈ 이상인 특성을 나타낸다. 설계된 칩은 ETRI MESFET 0.5 ㎛공정으로 제작하였으며 칩 사이즈는 1.4㎜×1.4㎜이다 This is paper describes a design and fabrication of a one-chip receiver MMIC for IMT-2000. The LNA, double balanced mixer, LO balun, RF balun, IF amplifier and a bias circuit are integrated a chip. Each balun operates active and composed of MESFETs. The new applied bias circuit is able to compensate the variation of the threshold voltage caused by the process variation, temperature changes, and etc. The measured response of the fabricated chip doesn't correspond with the simulated performance, the LNA is not only the conversion gain doesn't operates flatly, but also even the oscillation presented. By the study of the cause about the problem, the factor was found. And the result will be deal with further study. The mixer achieves the conversion losses of -14㏈, IP3 of 4㏈m, and port to port isolation over 25㏈. The designed chip is fabricated by the ETRI MESFET 0.5㎛ process. The chip size is 1.4m×1.4m.

극저준위 감마선 분광시스템의 백그라운드 저감화를 위한 중성자 차폐체 설계

김태욱,박종묵,박종길,신상운,전재식 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.2

극저준위 방사능측정시스템의 백그라운드에 영향을 주는 중성자를 차폐하기 위한 차폐체를 설계하였다. 중성자 차폐방법은 고 밀도 폴리에틸렌을 이용하여 고속중성자를 감속한 후 B4C를 이용하여 감속된 열중성자를 흡수하는 방법을 이용하였다. 몬테카를로 모사방법인 MCNP4B 코드를 이용하여 계산한 결과 고 밀도 폴리에틸렌의 두께가 10 cm 일 때 열중성자속이 최대가 되는 것으로 나타났으며 감속된 중성자의 흡수는 용제에 자연상태의 B4C 분말을 30 w% 섞을 경우 2 mm의 두께에서 94%의 중성자 흡수가 일어나는 것으로 나타났다. 또한 몬테카를로 모사를 통한 계산결과의 타당성 여부를 조사하기 위하여 중성자 차폐실험 장치를 제작하여 실험 결과와 비교하였으며, 비교 결과 실험값과 일치하는 것으로 나타났다 In order to shield the neutrons affecting the background of Low Level Gamma Ray Spectrometer, a neutron shielder was designed. The method used in this study for neutron shielding was the deceleration of fast neutrons by high density polyethylene(HDPE) and the absorption of those slowing-down neutrons by B4C. The calculation results of neutron interaction in HDPE using Monte Carlo simulation code MCNP4B showed that the thermal-neutron flux was maximum at 10 cm thickness of HDPE. The results also showed that 95% of the thermal neutrons were absorbed by 2 mm thickness of B4C absorber consisted of 30 w% B4C and 70 w% polymer. The results of the Monte Carlo calculation were in good agreement with the experimental value obtained by a neutron shielding apparatus designd for this purpose.

Disease-miRNAdb: a manually-curated database for the investigation of the microRNA-human disease relationship

Shin, Jae Moon,Lim, Wooyoung,Lee, Kyung-Mi,Won, Jung Im,Jung, Da Woon,Nho, Chu Won,Kang, Kui Dong,Yoon, Jee Hee,Cho, Yoon Shin Springer-Verlag 2017 Genes & Genomics Vol.39 No.12

<P>MicroRNAs (miRNA) of approximately 22 nucleotides are fundamental molecules in cellular biology that act by modulating target gene expression. As a post-transcriptional/translational regulator, the role of miRNAs in human disease is highly expected. Recently, a rising number of miRNAs that are associated with a variety of human diseases have been reported in the literature. Aiming to provide a comprehensive of resource of miRNAs that are related to the human disease, we built the Disease miRNA Database (Disease-miRNAdb). MiRNAs from three groups of diseases including kidney disease, cancer, and metabolic disease were manually curated in the current version of database located on a user-friendly online website (http://disease-miRNAdb.hallym.ac.kr). In addition, this database also includes information regarding single nucleotide polymorphisms (SNPs) located in pre-miRNAs, miRNA flanking regions, and 3'-UTRs of miRNA target genes. The generation of genetic variation in these regions may result in a loss or gain of miRNA-target gene interactions, which may influence the development of disease. Thus, information regarding miRNA-related SNPs will be useful in the discovery of disease-associated miRNAs in human populations by employing SNP association analysis for the disease of interest. Disease-miRNAdb will be continually maintained with up-to-date information to provide a current and valuable resource for investigating the roles of miRNAs in human disease.</P>

Multiple subcutaneous nodules leading to the diagnosis of pulmonary aspergillosis in immunocompromised patient with COPD (초)

( Jae Woo Jung ),( Sung Woon Park ),( Eun Ju Jun ),( Jae Chol Choi ),( Jong Wook Shin ),( Jae Yeol Kim ),( In Won Park ),( Byoung Whui Choi ),( Mi Kyung Kim ) 대한내과학회 2008 대한내과학회 추계학술대회 Vol.2008 No.-

Free Paper Session : HCC ; Risk factors for Liver abscess after TACE in patient with HCC (초)

( Jae Uk Shin ),( Kwang Cheol Koh ),( Shin Young Min ),( Sang Un Park ),( Geum Youn Gwak ),( Moon Seok Choi ),( Joon Hyoek Lee ),( Seung Woon Paik ),( Byung Chul Yoo ),( Sung Wook Shin ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3(S)

A Case of Cutaneous Vasculitis with Acronecrosis in a Neonatal Dog

Jae-Ik Han, Young-Mi Shin, Hee-Woon Lee, Byeongwoo Ahn, Ki-Jeong Na 충북대학교 동물의학연구소 2010 Journal of Biomedical and Translational Research Vol.11 No.2

본문참고

Signal Change of Normal Saline by Oxygen Injection in FLAIR Image

Woon-Jae Shin 한국방사선학회 2019 한국방사선학회 논문지 Vol.13 No.1

고농도의 산소를 흡입하는 경우에 자기공명영상의 FLAIR(fluid attenuated inversion-recovery, FLAIR) 영상 에서 뇌척수액에서 신호가 억제되지 않고 고신호로 나타나는 경우가 있다는 보고가 있었다. 본 연구는 아가 젤로 고정한 팬텀을 제작하여 산소를 주입한 생리식염수와 조영제를 희석한 생리식염수의 신호를 FLAIR 기법의 반전시간(TI : inversion time)을 변화하여 영상을 획득하고, 분석하여 기초자료를 마련 하고자 하였다. 부산 P병원의 Philips Achieva MR 3.0T를 이용한 결과에서 자기공명영상의 FLAIR 기법에서 산소가 주입된 생리식염수의 신호 대 잡음비(signal to noise ratio)는 산소가 주입되지 않은 생리식염수 보다 증가되었다. 하지만 희석된 조영제보다는 높지 않았다. 반전시간 1,800ms에서는 산소에 의한 신호 증강이 없는 영상을 획득할 수 있었다. 산소가 주입된 생리식염수와 조영제의 대조도 대 잡음비(contrast to noise ratio)에서도 임상에서 주로 사용하는 반전시간 2,800ms보다 1,800ms에서 높게 증가되었다. 본 실험의 결과가 자기공명영상의 FLAIR 기법에서 산소 주입에 따른 뇌척수액의 신호 변화 연구에 기초자료가 될 수 있을 것으로 사료된다. It was reported that there were some cases in which signal was not inhibited but high signal appeared in cerebrospinal fluid on FLAIR(fluid attenuated inversion recovery) of MRI(Magnetic Resonance Imaging) in case a person inhales high-concentration oxygen. This study was to prepare basic database. We produced a phantom fixed with agar gel and by using it, obtained the images of the signals of normal saline into which oxygen was injected and normal saline diluted with contrast media by changing the TI(Inversion Time) of FLAIR technique and analyzed them. In the result of FLAIR technique of MRI using Philips Achieva MR 3.0T in Busan P Hospital, the SNR(Signal to Noise Ratio) of normal saline into which oxygen was injected was higher than the SNR of normal saline into which oxygen was not injected. However, it was not higher than the SNR of normal saline diluted with contrast media. In the TI 1,800ms, we could obtain the images which do not have the rise of the signal due to oxygen. In the CNR(Contrast to Noise Ratio) of normal saline into which oxygen was injected and normal saline diluted with contrast media as well, it was higher in the TI 1,800ms than in the TI 2,800ms that is mainly used clinically. It is thought that the result of this study could be basic database for studies on change of signal of cerebrospinal fluid as a result of injection of oxygen in FLAIR technique of MRI.

Multiple subcutaneous nodules Leading to the diagnosis of pulmonary aspergillosis in immunocompromised patient with COPD (초)

( Jae Woo Jung ),( Sung Woon Park ),( Eun Ju Jun ),( Jae Chol Choi ),( Jong Wook Shin ),( Jae Yeol Kim ),( In Won Park ),( Byoung Whui Choi ),( Mi Kyung Kim ) 대한내과학회 2008 대한내과학회지 Vol.75 No.1S

Development of BPH Resistant Rice Variety for Environmental Friendly Cultivation

Woon-Chul Shin,Ki-Young Kim,Jong-Cheol Ko,Young-Jun Mo,Jeong-Kwon Nam,Mun-Sik Shin,Bo-Kyeong Kim,Jae-Kwon Ko 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

BPH(Brown planthopper, Nilaparvata lugens Stål) is a major insect of rice and give a lot of damages around Korea, Japan and East-West Asia. BPH often causes heavy losses of up to 60% of rice crop yields in susceptible cultivars. Especially, increase of the environmental friendly cultivation in South Korea has caused the outbreak of BPH rapidly. But few resistant varieties in Korea have known until now. Utilization of R genes in breeding programs has been the most effective and economical strategy for controlling insect resistance. It is reported that Bph1 gene is located on a long side of chromosome 12 and linked to bph2 among reported 18 R genes. BPH resistant "Iksan529" was derived from a cross between HR22538-GHB-36-4(bph2) and Iksan471 at Department of Rice and Winter Cereal Crop, NICS, RDA. Culm length of Iksan529 is 74cm and it shows moderate resistance to blast, and wide spectrum resistance to bacterial blight pathogen(K1, K2, K3) and stripe virus. Especially, Iksan529 was identified bph2 gene using KPM2(co-dominant CAPS marker) and KPM4(resistant-associated marker). When Iksan529 seedling were infested at 2nd and 3rd instar stages of BPH nymphs(Bio-type 1), it showed resistance at one month after infest. Iksan529 will be tested local adaptability for 3 years and deveoped for environmental friendly cultivation.