야생효모, Wickerhamomyces anomalus HO9-2로부터 급성 폐손상 억제물질인 호중구 elastase 저해물질 생산 및 생산균주의 균학적 특성

장지은, 문정수, 김하근, 이종수 배재대학교 자연과학연구소 2023 自然科學論文集 Vol.34 No.1

This study was performed to produce acute lung injury-preventing neutrophil elastase inhibitor from non-pathogenic wild yeast and further determine enzyme activity and physiological functionality of the potent wild yeast. Culture supernatants and cell-free extracts of one hundred twenty five non-pathogenic wild yeast strains were prepared and their neutrophil elastase inhibitory activities were measured. The neutrophil elastase inhibitory activity of the cell-free extract from Wickerhamomyces anomalus HO9-2 showed the highest inhibitory activity of 38.4% and its elastase inhibitor was maximally produced(IC50,3.2㎎) when Wickerhamomyces anomalus HO 9-2 was cultured in pH 7.0 of malt extract medium at 20℃ for 24h. Wickerhamomyces anomalus HO9-2 was oval shape and did not form ascospore and pseudomycelium. The HO9-2 strain grew well in YPD and YM media and was halophilic yeast which grew well in YPD medium containing 15% NaCl. Cell-free extract of the HO9-2 strain were showed high 17.2U of glucoamylase activity and anti-diabetic α-glucosidase inhibitory activity of 87.5%. 본 연구는 급성 폐손상 예방(또는 치료)의약 소재를 개발하기 위하여 비병원성 야생효모들의 elastase 저해활성을 측정하여 우수 균주를 선발하였다. 또한 선발균주의 elastase 저해제 생산 조건을 최적화 하였으며 선발균주의 미생물학적 특성 등을 조사하였다. 비병원성 야생효모인 Wickerhamomyces anomalus HO9-2의 무세포추출물이 38.4%의 호중구 elastase 저해활성을 보여 우수균주로 선발하였고 이 균을 pH 7.0 의 malt extract 배지에서 20℃로 24시간 배양하였을 때 최고의 elastase 저해활성(IC50, 3.2mg)을 보였다. 또한 이 균은 난형으로 포자와 의균사를 형성하지 않았고 YPD와 YM배지에서 생육이 양호하였고 NaCl을 15% 함유한 배지에서도 생육하는 호염성 효모이었다.

축구포지션별 무산소 운동능력 분석

손장식,정정욱,김종호,임정수,김훈 대구대학교 기초과학연구소 2003 基礎科學硏究 Vol.19 No.3

In this study, the Wingate power test was performed to investigate of the anaerobic power output in soccer players who play in different position. The main purpose of the test was to provide the enhancement of anaerobic capacity which was conducted by comparing and analysing of the Wingate test. This will improve the performance during the soccer games. Seventeen soccer players with three different positions, FW, MF, DF, were recruited, and undertook the Wingate power test, and anaerobic power, peak power output, anaerobic capacity, and fatigue index was analysed. There was a significant effect of a peak power output among positions showing MF>DF>FW (P<.05). MF revealed the highest peak power output at 15 and 30 seconds time points compared to DF and FW. As far as the players' positions were concerned, a relative anaerobic capacity showed a similar results with a peak power output (P<.05; MF>DF>FW). Unlike with these results, the fatigue index in different positions did not show any statistical significant results (P>.05). Based on these results, it was suggested that the systematical training program is required to perform a modern soccer, pressure and total soccer, in MF players. If the physical strength, especially power out, in MF players is enhanced, the results of the soccer games will be also improved.

풍력발전기 원격모니터링 시스템 구축 및 개발

차장현,이정완,유능수,남윤수 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.A

In this paper, remote monitoring system for wind turbine is developed. The developed system consists of data acquisition for wind sensor, and monitoring for site environment. In order to accomplish effective monitoring, the system uses Datasocket, SMB, FTP, Web Server, and G Web Server. Two computer system - one is data acquisition computer using Windows-XP and the other is monitoring computer using UNIX - constraint the distribute system with individual tasks. By using this system, one can perform various monitoring and control tasks in Wind-Turbine application, efficiently.

분만 후 심한 저혈당이 동반된 림프구성 뇌하수체염 1예의 경과 관찰

손장명,고승현,안유배,주강,이정록,양성은,송기호,손호영,전정수 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

저자들은 출산 후 발생한 심한 저혈당을 주소로 내원한 31세 여자 환자에서 뇌 자기공명영상검사에서 뇌하수체종괴가 관찰되고 복합 뇌하수체 기능검사 결과전반적인 뇌하수체 기능저하증의 소견을 보여 림프구성 뇌하수체염을 진단하고, 호르몬 보충요법만으로 경과를 관찰하여 호전된 예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Lymphocytic hypophysitis, an inflammatory disease of the pituitary gland that is often associated with pregnancy, is caused by autoimmune destruction of the pituitary gland evidenced by diffuse inflammatory cell infiltration of the pituitary gland and some kinds of detectable autoantibodies. We report a case of lymphocytic hypophysitis in a 31-year-old woman presenting with severe hypoglycemia and hyponatremia after delivery. Hormonal study revealed panhypopituitarism and magnetic resonance imaging with enhancement showed the bulging contour of the right side pituitary gland with an ill-defined mass-like lesion and nodular thickening of the stalk The patient's symptoms and biochemical data improved greatly with replacement of L-thyroxine and glucocorticoid. Partial recovery of panhypopituitarism was also seen. The follow-up tests revealed dramatic resolution of the pituitary lesion (J Kor SOC Endocrinol 1 8 : 3 25 -33 1, 2003).

풍력발전기 원격모니터링 시스템 구축 및 개발

차장현,이정완,유능수,남윤수 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

In this paper, remote monitoring system for wind turbine is developed. The developed system consists of data acquisition for wind sensor, and monitoring for site environment. In order to accomplish effective monitoring, the system uses Datasocket, SMB, FTP, Web Server, and G Web Server. Two computer system - one is data acquisition computer using Windows-XP and the other is monitoring computer using UNIX - constraint the distribute system with individual tasks. By using this system, one can perform various monitoring and control tasks in Wind-Turbine application, efficiently.

石灰石 添加에 따른 二酸化黃 除去率 向上

김영수,박진식,장성호,장부규,서정민,김장호 동아대학교 환경문제연구소 1996 硏究報告 Vol.19 No.2

This study was carried out to investigate the performance enhancement of forced oxidation operation on the limestone utilization and SO₂ removal efficiency of the pilot flue gas desulfurization system. The results are as follows : 1. The results of the forced oxidation tests have shown that converting from natural to forced oxidation operation can have a significant effect on both limestone utilization and SO₂ removal efficiency of an FGD system. Conversely, if the limestone utilization is maintained at a constant level, the SO₂ removal efficiency will increase. Conversely, if the pH is held constant, the limestone utilization will increase significantly. 2. This characteristic of a forced oxidation system can translate into lower L/G requirements and, therefore, reduced capital and operating costs for slurry recycle pumps. 3. Forced oxidation operation will provide improved limestone utilization and, therefore. reduced limestone reagent costs.

풍촌 석회암의 소성에 관한 연구

이재장,노범식,장경호,정수현 강원대학교 석재복합신소재제품연구센터 1997 석재연 논문집 Vol.2 No.-

본 연구는 여러 가지 용도에 적합한 생석회를 생산하기 위하여 풍촌 석회석을 대상으로 소성시험을 하였다. 시료의 형상은 주로 정방형이나 때로는 구형을 나타내며, 배색 내지 회백색을 띈다. 소성시험에 사용한 시료의 크기는 45∼50㎜ 이며, 소화시험에서는 3.3mm(6mesh) 이하로 분쇄하여 소성하였다. 시료의 광물학적 특성을 보기위하여 편광현미경 관찰, X-ray 분석, 열분석을 하였다. 시료는 거의 순수한 CaCO₃이었으며, 600℃ 부터 열분해가 시작되어 850℃에서 완결되었다. 화학분석 결과 CaO 98.27%인 잠재적 순도를 갖는 생석회를 얻을 수 있음을 알 수 있다. 대략 50 ㎜ 정도의 석회석은 전기로의 온도가 실온에서 1,050℃에 도달하였을 때 99%이상이 소성되었다. 이 때 얻어진 생석회는 거의 순 백색이며 갈라짐이 없었다. 소화시험 결과 ASTM C-110에 의하면 반응성이 우수한 생석회인 것을 알 수 있으며, 1분 이내의 상승온도는 63℃ 이었다. In this study, the calcination characteristics of Pungchon limestone was investigated to produce the optimum quick lime for various uses. The shape of limestone sample was mainly cubic, but sometimes longish. Approximate size range for the calcination tests was from 45 to 50 mm. For the slaking test, it was crushed by cobbing method and screened by 3.3 mm(6 mesh) screen. Mineralogical studies of the sample were carried out to investigate textures, X-ray diffraction analysis and thermal analysis. The result of chemical analysis shows a very high grade limestone with a CaO potential of 98.27%. Approximate 50 mm size's of limestone, about 250 g was calcined more than 99% when the calcination temperature arrived at 1,050 ℃ from room temperature in the electric laboratory furnace. Lumpy lime in nearly white colour with nearly no cracks was observed during calcining test. and during calcination process the limestone places kept their shape. Slaking test(ASTM C-110 ) results indicate a high reactivity product. In slaking, a 63℃ temperature rise was achieved within the first one minutes.

한국어 판 구조화 임상면담도구 개발 : 신뢰도 연구

한오수,안준호,송선희,조맹제,김장규,배재남,조성진,정범수,서동우,함봉진,이동우,박종익,홍진표 대한신경정신의학회 2000 신경정신의학 Vol.39 No.2

연구목적: 정신장애의 정확한 진단과 평가는 임상에서뿐만 아니라 신뢰성 있는 연구를 위해서도 매우 중요하다. Structured Clinical Interview for DSM-IV(SCID)는 임상전문가들이 사용하는 진단도구로서, 비교적 짧은 시간에 정확한 진단을 내릴 수 있다. 본 연구는 한국어판SCID를 개발하고 그 신뢰도를 평가하기 위해 수행되었다. 방법: 국문학자가 포함된 번역위원회를 통하여 연구용판 SCID를 번역한 후, 정신과 의사 2인과 임상심리학자 1인에게 한국어판SCID 실시방법을 교육시킨 뒤 한국어판SCID를 이용한 면담의 평가자간 신뢰도 (interrater reliability)를 검증하였다. 면담 대상은 1999년 2월에서 3월까지 2개 병원 정신과에 치료중인 환자 90명(남:41명, 여:29명)이었다. 결과: 현재 장애(current disorder) 평가에서 주요 우울장애, 기분부전장애, 정신분열병, 알코올 남용 및 의존, 기타 물질 남용 또는 의존, 여러 불안장애들 및 섭식장애 등과 같은 대부분 장애의 kappa값은 .70이상으로 매우 높았다. 그 이외의 양극성 장애, 망상장애, 광장공포증, 감별 불능 신체화 장애 및 건강염려증의 kappa값도 .69에서 .40사이로 수용할 수 있는 정도였다. 평생 장애(lifetime disorder)에서는 양극성 장애(k=.69)와 감별 불능 신체화장애(k=.59)를 제외한 다른 모든 장애의 kappa값이 .70이상이었다. K-SCID 면담시 Ⅰ축 질환에 대하여 흔하게 다중 진단이 내려졌으며, 평균 진단 수는 1.5∼1.7개로 나타났다. 결론: 한국어판 SCID는 신뢰도가 높은 진단도구로 생각되며, 향후 정신질환의 정확한 진단과 임상연구에 유용하게 사용될 수 있다. Objectives: Accurate diagnosis and assessment for psychiatric disorders is crucial for research, as well as for clinical practice. Structured Clinical Interview for DSM-Ⅳ(SCID-RV) is a less time-consumimg and more accurate structured diagnostic interview form. It can be used by clinical professions and is known for a reliable diagnostic tool. Present study was conducted to develop Korean version of SCID-RV and to test the inter-rater reliability. Methods: The authors have translated original SCID-RV into Korean, and revised in parallel with sociocultural background of Korea. Ninety patients from two psychiatric hospitals, both outpatient and inpatient, were interviewed and rated independently by three raters. Results: The kappa coefficients for most of illnesses, such as major depressive disorder, dysthymia, schizophrenia, alcohol abuse and dependency, anxiety disorder and eating disorder were excellent(>0.70) in the evaluation of current disorders. And the kappa coefficients for bipolar disorder, delusional disorder, agoraphobia, undifferentiated somatoform disorder, and hypochondriasis were acceptable(>0.40) in the evaluation of current disorders. In the evalua-tion of lifetime disorders, the concordant rates of all the diagnoses except bipolar disorder and undifferentiated somatoform disorder were excellent. Lack of hierarchy in DSM-Ⅳ allows for multiple Axis I diagnoses. Mean numbers of Axis I diagnoses per subject assigned by the three raters were 1.5-1.7. Conclusions: Our findings confirm that SCID-RV yields highly reliable diagnoses. SCID-RV is recommended for accurate diagnosis in clinical practice and research on psychiatric disorders.

수직축 블레이드를 적용한 소형 풍력발전시스템에 관한 연구

윤정필,최장균,차인수 동신대학교 2004 論文集 Vol.14 No.-

Need developments of substitute energy to solve problem of global warming by excess use of fossil energy, excess discharge of carbon dioxide. Wind Generation is all-important energy in next generation as clean energy. Environmental pollution of wind force development is not exhausted entirely. And, electric-power production unit cost is cheap than other energy. Wind Generation system that is supplied much present is most horizontality style blade structure. But, Horizontal style structure is serious noise and there is problem in stability of blade. We designed special blade aiming to this problem. And, manufactured vertical axis wind force development system because using blade. Also, developed assistance power generator to heighten driving efficiency ago wind generation. We expect this devices that is such cover shortcoming of wind force development.

비병원성 야생효모 Rhodosporidium toruloides HGG35-2와 호중구 elastase 저해제 생성효모인 Wickerhamomyces anomalus HO9-2로부터 Killer toxin 생산

문정수, 장지은, 김하근, 이종수 배재대학교 자연과학연구소 2023 自然科學論文集 Vol.34 No.1

항진균 바이오의약의 소재인 killer toxin을 비병원성 야생효모로부터 생산하기 위하여 25개 비병원성 색소 생성 효모들의 killer toxin 활성을 측정하여 우수균주로 Rhodosporidium toruloides HGG35-2와 Wickerhamomyces anomalus HO9-2을 선발하였다. 이들 killer toxin 생성균주들의 효모 감수성 균주들에 대하여 killer 활성을 측정한 결과 시판 포도주등의 알콜발효로 이용되고 있는 Saccharomyces cerevisiae (La Parisienne®)에 대하여 우수한 항진균활성을 보였다. Killer toxin production of non-pathogenic wild yeasts, Rhodosporidium toruloides HGG35-2 and Wickerhamomyces anomalus HO9-2 were investigated. Rhodosporidium toruloides HGG35-2 showed strong killer activity against pathogenic yeast, Candida guilliermondii AHP28-1 and commercial alcohol fermentation yeast, Saccharomyces cerevisiae(La Parisienne). Wickerhamomyces anomalus HO9-2 also showed killer activity against Rhodotorula nothofagi JSC10-1 and commercial alcohol fermentation yeast, Saccharomyces cerevisiae(La Parisienne®).