Improved Micropropagation of Root Chicory, Cichorium intybus L. var. sativus.

Lim,Jung-Dae,Yang,Deok-Chun,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Sung,Eun-Soo,Yu Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1

The establishment of an efficient protocol for plant regeneration and micropropagation from leaf explant cultures of Chicory, Cichorium intybus L. var. sativus. is reported. Callus formation rate appeared 100% from explant in all growth regulators, but calli formed in the prensence of naphthaleneacetic acid (NAA) were appeared very compact and non-embryogenic state. The regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The highest number of shoots (5.7) per explant and shoot growth (2.8cm) was obtained on MS medium containing 0.1 mg BAP L^-1 and 0.1 mg NAA L^-1. Indole acetic acid was the most suitable auxin for root formation among three auxins tested. 2,4-D had no effect on shoot and root formation.

혼수상태의 외상환자에서 초음파를 이용한 흉부 및 복부손상의 진단

임경수,이강현,이진웅,이부수,황성오,유수영,강성준 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

The need for rapid diagnosis and treatment of life-threatening thoracic and intra-abdominal injury result in controversy over the appropriate triage of unconscious blunt trauma patients. To aid in early decisions for these patients, a prospective analysis of 98 patients with glasgow coma scale(GCS) scores≤8 was undertaken. Although intra-thoracic and intra-abdominal injuries were frequently identified based on systolic blood ressure, the use of clinical signs alone resulted in more missed injuries than did using the emergency ultrasonography. In normotensive patients(n=34), intra-thoracic injuries was identified in one patient(2.9%), and intra-abdominal injuries were in 7 patients(20.6%). In shock(systolic blood pressure<90mmHg) Pa-tients(n=64), thoracic injuries and intra-abdominal injuries were diagnosed in 8(12.5%) and 27 patients(42.3%), thoracic and abdominal combined injuries were identified in 11 patients(17.2%). The sensitivity, specificity, and accuracy of emergency ultrasonography were 96.3%, 91.7%, 94.9%. This study suggests that all unconscious trauma patients undergo immediate emergency ultrasonography to prevent missing life-threatening injuries.

安東地域住民의 生活體育에 대한 意識構造와 活性化方案

林銖遠,朴根守,張龍洙 慶北大學校 師範大學 體育學硏究會 1992 體育學會誌 Vol.20 No.-

The primary purpose of this study was to elucidate structure of consciouness about An-Dong Citizen's life be in sports and grope a device of activation. In order to meet this purpose, 977 questionnaires was analyzed according to sex, age, education, occupation and income. The results were as follows; 1) 84.4% of whole subjects felt necessity of Life be in Sports in every day life. in view of sex, 87.3% of males and 81.5% of females felt the necessity. 2) 81.8% of subjects regarded life be in sports as means of health improvement. 3) About the local facilities of life be in sports, only 9.4% of subjects satisfied and 64.9% of subjects revealed dissatisfaction. 4) Preference order in relation to the expansion of facilities appeared as follews; swimming pool(26.9%), tennis court(18.6%), gymnasium(11.4%). badminton court(11.5%), aerobic hall(5.8%) and bowling range(4.2%). 5) About making sport club, 82.4% of Whole subjects revealed affirmative answer. 6) About present participation in life be in sports, 30.1% of subjects answered being participated, but 69.9% subjects answered not being participated. 7) About necessity of coach of Life be in soprts, 66.0% of subjects felt the necessity. 8) The main reason of not attendance in Life be in sports were lack of leisure, economical difficulty, insufficiency of facilities. 9) 13.0% of subjects wanted to participate in tennis, 11.7% to swimming, 11.3% to badminton, 8.6% to mountaineering, 7.6% to aerobic, and 6.9% to bowling if circumstance permit. and then, the major device for activation of life be in sports were utilization and expansion of facilities, development of program, arrangement and nurture of coach, public information about life be in sports, support and construction of sports club, financial and administrative support.

The Public Policies for the Rural Infrastructure Improvement in Korea : Comparison with the US and Japan

Lim,Kyoung-Soo 한국지역개발학회 1996 韓國地域開發學會誌 Vol.8 No.2

당뇨병 환자에서 혈장 Thrombin-Antithrombin Ⅲ 및 Plasmin-α_2-Plasmin Inhibitor 복합체의 임상적 의의

김경욱,김은숙,정상수,윤수지,박우일,이준희,남수연,안철우,문병수,김경래,차봉수,송영득,임승길,이현철,허갑범 대한당뇨병학회 2002 Diabetes and Metabolism Journal Vol.25 No.5

연구배경:당뇨병 환자에서 혈액응고 및 섬유소용해 체계의 이상경향이 있어 그 결과로 여러 혈관합병증의 발생위험이 높다는 사실은 널리 알려져 있다. 그 기전은 아직 확실히 밝혀지지 않았으나, 고혈당으로 인한 혈장 단백질들의 비효소성 당화작용이나 산화성 스트레스로 인한 유리 라티칼 작용으로 응고항진이나 섬유소용해 활성의 저하를 유발하는 것으로 생각되고 있다. 최근 응고 및 용해인자와 그 억제자의 복합체들의 증가가 이 상태를 비교적 예민하게 반영한다고 알려져 있다. 방법:본 연구에서는 당뇨병 환자 101명과 정상 대조군 20명에서 혈장내 thrombin­antithrombin complex(TAT)와 plasmin­α₂­plasmin inhibitor complex(PIC)를 측정하여 비교하고, 당뇨병 환자에서 미세혈관 합병증과 대혈관합병증의 유무에 따른 차이와, 이미 혈관 질환의 위험인자로 알려져 있는 인자들간의 상관성을 알아보고자 하였다. 결과:1. 환자의 분포를 살펴보면 혈관합병증이 있는 군은 85명, 혈관합병증이 없는 군은 16명이었고, 평균연령은 각각 57.9±14.1세, 49.9±16.6세로 혈관 합병증이 있는 군에서 더 나이가 많았고, 체질량지수는 23.2±3.4㎏/㎡, 24.1±3.4㎏/㎡로 두 군간 유의한 차이는 없었다. 또 두 군간의 혈압 및 HbA1c, 공복혈당 및 인슐린과 C­peptide, 총 콜레스테롤, 중성지방, HDL­콜레스테롤, Lp⒜는 유의한 차이가 없었고, 미세혈관합병증이 있는 군에서 당뇨병의 유병기간이 길었다. 2. TAT 및 PIC의 농도는 정상 대조군에서는 2.8±1.2 ng/mL, 240.4±69.7 ng/mL이었고, 당뇨병 환자군에서는 9.5±22.6 ng/mL, 472.2±258.7 ng/mL이었다. TAT와 PIC 모두 당뇨병 환자군에서 정상 대조군에 비해 유의하게 증가되어 있었고(p<0.001), TAT/PIC ratio는 두 군간 차이가 없었다. 3. 당뇨병 환자의 혈관합병증에 따른 TAT 및 PIC, fibrinogen 농도는 합병증이 없는 군은 각각 4.1±2.4ng/mL, 362.2±272.0ng/mL, 322.7±102.4mg/mL으로 PIC와 fibrinogen의 증가를 보였으나, 연령을 보정한 후에는 통계학적 유의성은 없었다. 또 대혈관 합병증군에서는 각각 6.0±4.9 ng/mL, 507.4±321.6 ng/mL, 427.1±194.7 mg/dL이었으며 미세·대혈관 합병증군에서는 10.4±6.4 ng/mL, 484.8±269.7 ng/mL, 388.4±132.4 mg/dL으로 TAT의 증가를 보였으나 역시 연령을 보정한 후에는 통계학적 유의성은 없었다. 4. 미세혈관합병증군에서 HbA1c(>8%)가 높은 군의 PIC 농도가 유의하게 높았고(p=0.049), 대혈관합병증군에서 HbA1c(>8%)가 높은 군의 총 콜레스테롤 농도가 유의하게 높았다(p=0.042). 5. 총 당뇨병 환자군에서 PIC는 fibrinogen과 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며(r=0.47, 0.31,-0.25), 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다(r=0.67). 결론:이상의 결과에서 혈장 TAT 및 PIC 농도는 당뇨병 환자에서 정상 대조군에 비해 의미있게 증가되어 있었고, 당뇨병 환자군에서는 연령의 증가와 유병기간이 혈액응고항진 및 용해의 장애에 큰 역할을 함을 알 수 있었으며, 총 당뇨병 환자군에 PIC와 HbA1c와 양의 상관관계를, BMI와 음의 상관관계를 보였으며 혈관 합병증이 없는 당뇨병 환자군에서만 TAT는 HbA1c와 양의 상관관계를 보였다. 따라서 당뇨병 환자에서 혈액응고 및 용해의 장애가 동반되어 있다고 볼 수 있으며, 혈장 TAT 및 PIC는 혈관합병증으로의 진행을 예측하는 지표로서 유용하리라 생각된다. 또 혈당조절정도와 상관성이 있으므로 혈당조절후에 추적검사를 시행하여 합병증의 예방이 가능한지 추후 연구가 필요하리라 생각된다. Background : Abnormality of coagulation and fibrinolystic system is known as a predisposing factor of vascular complication in diabetes. Although the pathogenesis is not well known, non-enzymatic glycation reaction and the increase in production of free radicals due to an increased oxidative stress may be linked to the hypercoagulibility and hypofibrinolytic activity. As indices of abnormality in coagulation and firinolysis in peripheral blood, plasma thrombin-antithrombin Ⅲ complex (TAT) and plasmin-α_2-plasmin inhibitor complex (PIC) were measured. The purpose of this study was to clarify whether hypercoagulability exists in diabetic patients with or without vascular complication. Methods : In our study, we measured plasma thrombin-antithrombin Ⅲ compelx (TAT) and plasmin-α_2-plasmin inhibit or complex (PIC) in 101 diabetic subjects and 20 controls. Comparing TAT and PIC levels in diabetic microvascular complication group, diabetic macrovascular complication group and controls, we examined correlation between risk factors associated with diabetic vascular complication. Results : 1. The group with diabetic vascular complication was older than group without complication. There was no significant difference in BMI, blood pressure, HbA_ic, blood sugar level, insulin, C-peptide, serum creatinine, total cholesterol, triglyceride, HDL-cholesterol, Lp (a) between two groups. The group with diabetic microvascular complication had longer duration of diabetes. 2. Concentration of TAT and PIC were 2.8±1.2 ng/ mL, 240.4±69.7 ng/ mL in controls and 9.5±22.6 ng/ mL, 472.2±258.7 ng/ mL in diabetic patients, respectively. TAT and PIC were significantly higher in diabetic patients than in control (p<0.001). But TAT/PIC ratio was no significant difference between two groups. 3. In diabetic patients, concentration of TAT and PIC and fibrinogen were respectively 4.1±2.4 ng/ mL, 362.2±272.0 ng/ mL, 322.7±102.4 mg/ dL in group without vascular complication and 5.3±4.1 ng/ mL, 529.5±258.7 ng/ mL, 374.9±106.2 mg/ dL in group with microvascular complication, which group had increase in PIC and Fibrinogen but no significance after correction of age. Concentration of TAT and PIC and Fibrinogen were 60.±4.9 ng/ mL, 507.4±321.6 ng/ mL, 427.1±194.7 mg/ dL in macrovascular complication, and 10.4±6.7 mg/ mL, 484.8±269.7 ng/ mL, 388.4±132.4 mg/ dL in combined vascular complication which group showed increase of TAT but also had no significant increase after correction of age. 4. In diabetic microvascular complication patients, group of high HbA_1c (>8%) (p=0.049) had significant high PIC concentration. In diabetic macrovascular complication patients, group of high HbA_1c (>8%) (p=0.042) had significant high total cholesterol concentration. 5. In all diabetic patients, PIC was positively correlated with fibrinogen and HbA_1c and negatively correlated BMI (r=0.47, 0.31, -0.25). Only in daibetic patients without angiopathy, TAT was positively correlated with HbA_1c (r=0.67). Conclusion : In this study, plasma TAT and PIC concentration significantly increased in diabetic patients compared with controls, and PIC was increased in group with microvascular complication, TAT were increased in group with combined micro macrovascular complication. However, there was no significance relationship existed when correctinf for age. PIC was correlated with HbA_1c. TAT was correlated with HbA_1c only in the group without angiopathy. Abnormality of coagulation and fibrinolysis were combined in diabetes, plasma TAT and PIC can be used as an index of vascular complication. Also we found the correlation with the degree of the blood glucose control. Therefore we need follow up study for the possibility of prevention of vascular complication after controlling the blood glucose to age-matched patients (J Kor Diaabetes Asso 25:354~363, 2001).

무수프탈산을 취급하는 근로자들의 알레르기 질환 유병 실태

임현술,김수근,김기식 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.4

Phthalic anhydride(PA) is widely used in the production of alkyd and unsaturated polyester resins. It has been reported that some workers exposed to PA have developed dermatitis, rhinitis and asthma. The authors intended to investigate the prevalence of PA induced allergic diseases and to develop preventive measures of occupational diseases. Forty-five male workers from PA production and handling were selected as an exposure group. Forty-four male workers not handling PA at tie same factory were chosen as a reference group. A symptom questionnaire, doctor's examination, eosinophil count, serum-total IgE and PA-specific IgE were done on the subjects. There were no significant differences in age, educational level. tenure or smoking habits between groups. Most clinical symptoms were significantly more frequent among the exposure group than the reference group: sneezing(62.2%), rhinorrhea(57.8%), nasal stuffiness(53.3%), coughing(44.4%) and nasal itching(35.6%) were the major symptoms among the exposure group(p<0.01, p<0.05). There were no significant differences in eosinophil count and serum-total IgE between groups but the mean level in PA-specific IgE, in the exposure group, was statistically significantly higher than the reference group(p<0.01). Abnormal rates of eosinophil count, serum-total IgE and PA-specific IgE were not significantly different from each other. The prevalence rate was 68.9%(31 cases) for allergic rhinitis in the exposure group, statistically significantly higher than the reference group(p<0.01). The prevalence rate was 6.7%(3 cases) for bronchial asthma and contact dermatitis in the exposure group, not more statistically significant than the reference group. In the exposure group, the prevalence rate for age, educational level, tenure, smoking habits and use of protective devices are not statistically significant. In PA specific IgE, sensitivity is 10.8%, specificity is 100.0%, positive predictability is 100.0% and negative predictability is 61.2% when criteria point is 3.5 KU/ιor above. As a result, it was recognized that the exposure group had a high prevalence of PA induced allergic rhinitis. Also, in diagnosis of PA induced allergic diseases, medical histories and specific symptoms were the most important where as eosinophil count, serum-total IgE are the only indirect informations. PA-specific IgE has been needed to evaluate the meanings more.

법의학적 견지에서의 약진의 임상적 의의

임수덕 大韓法醫學會 1981 대한법의학회지 Vol.5 No.1-2

爐보수 근로자의 혈중 Carboxyhemoglobin 농도와 신체증상

임현술,윤덕로,조수헌,김헌 大韓産業醫學會 1993 대한직업환경의학회지 Vol.5 No.1

제철소 爐보수 작업에 종사하는 근로자의 일산화탄소 폭로정도를 추정하고, 만성적인 저농도 일산화탄소 폭로가 근로자의 건강에 미치는 영향을 평가하고자 제철소 현장에서 일하는 로보수작업 생산직 근로자 808명을 폭로군으로, 제철소 밖에 있는 사무실에서 근무하는 근로자 162명을 대조군으로 하여 흡연, 작업조건, 신체증상 등을 묻는 설문조사를 시행하고 정맥혈을 채취하여 Co-oximeter(Instrumentation Laboratory사, Model482)를 이용하여 carboxyhemoglobin 농도를 측정하여 다음과 같은 결과를 얻었다. 1) 폭로군과 대조군의 혈중 COHb농도는 폭로군이 평균 3.21%, 대조군이 2.36%로 양군간에 통계적으로 유의한 차이를 보였다(p-value<0.0001 by T-test). 2) 흡연 습관에 따른 혈중 COHb농도는 흡연자군이 평균 3.88%, 비흡연자군이 0.90%로 양군 간에 통계적으로 유의한 차이가 있었으며(p-value<0.0001 by T-test) 폭로군과 대조군에서 모두 흡연자와 비흡연자간의 혈중 CPHb 농도는 유의한 차이를 보였다(p-value<0.001 by T-test). 3) 흡연자군의 혈중 COHb 농도는 폭로군과 대조군 사이에 유의한 차이가 있었으나 (p-value<0.05) 비흡연자군에서는 양군간에 유의한 차이를 발견할 수 없었다. 4) 폭로군이 대조군보다 통계적으로 유의할 정도로 더 높은 발현율을 보였던 증상은 없었다. 흡연자군에서는 비흡연자군에 비하여 '가래'의 발현율이 더 높게 나타났다(p-value<0.001 by χ²-test). 5) 혈중 COHb 농도는 작업종료후 채혈까지의 시간에 따라서는 유의한 변화양상을 보이지 않았던 반면 마지막 흡연후부터 채혈까지의 시간이 길어질수록 통계적으로 유의하게 감소하는 경향을 보였다. 이상의 결과로 미루어 제철소 로보수 작업 근로자의 일산화탄소 폭로수준은 임상적 증상을 유발할 만큼 높지 않으며 폭로군과 대조군의 혈중 COHb 농도가 통계적으로 유의한 차이를 보였던 것은 거의 대부분이 흡연습관의 차이에 기인하는 것으로 생각된다. 그러나 고위험군이나 흡연자의 경우에는 저농도 일산화탄소 폭로에 의해서 건강 장해가 유발될 가능성이 높으므로 저농도 일산화탄소에 폭로될 수 있는 작업장에서는 고위험군의 색출과 금연에 대한 교육이 필수적이다. To estimate carbon monoxide(CO) exposure levels and to evaluate health effects of chronic low level CO exposures of furnace-repairing workers, self-administrative questionnaire and venous carboxyhemoglobin(COHb) concentration measurements were performed on 808 furnace-repairing workers(exposed group) and 162 clerical workers(control group). Mean COHb level of furnace-repairing workers(3.21%) was significantly higher than that of clerical workers(2.35%)(p-value<0.0001 by T-test) and mean COHb level of smokers(mean:3.88%) was higher than that of nonsokers(mean:0.90%)(p-value<0.0001 by T-test). Smokers in exposed group showed statistically different COHb levels from those in control group but mean COHb level of nonsmokers in exposed group was not statistically different from that of control group. There was no CO-related symptoms, the positive response rates of which were higher in exposed group. 'Sputum' was the only symptom that showed higher positive rate in smokers. COHb levels did not linearly decrease with the time interval from workshift to blood sampling, while it did with time interval from last smoking to blood sampling. These results suggest that COHb level of furnace-repairing workers mainly depends on the smoking habit and may be related to their occupational CO exposures only to a small degree. Since low level CO exposure may be harmful to high risk patients and to smokers, furnace-repairing compaines require attention to detecting those high risk workers and educating hazardous effects of smoking.

전단보강근이 없는 재생골재 철근콘크리트보의 전단성능

임경택,정수영 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.1

Experimental investigation was conducted to study the shear capacity of recycled aggregate concrete beams without shear reinforcement. The primer objective of the study was t investigate the suitability of using recycled aggregate concrete beams. In designing the specimens, shear-span/depth ratio and aggregate consideration(washed or non-washed aggregate) were chosen as main parameters. As a result, this study, to know the shear capacity of recycled aggregate concrete beams with the main variables such as shear span ratio and weather the recycled aggregate was washed or not, following conclusions come out. 1.In the 3-point flexural strength examine certain comparison of flexural strength between natural and recycled aggregate concrete wasn't come out, because it is failure specimen with early flexural crack simultaneously. 2.Shear strength of recycled-aggregate concrete beam under shear span ratio a/d=3 was higher than ACI(318-95) Code, but lower over a/d =4. 3.Ultimate shear strength of recycled aggregate concrete beam was higher than ACI(318-95) Code and it was very high on shear span ratio a/d =2.

철강업체와 용접봉 제조업체에 근무하는 생산직 근로자의 직업성 요통 유병률과 관련 요인

임현술,김수근,김덕수,김두희,이종민,김양호 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.1

A cross-sectional study was conducted for detecting the risk factors and to propose an effective control program for occupational low back pain. The subjects were 1,665 male production workers employed at a steel factory and a welding material manufacturing factory. The data were collected by a self-reported questionnaire and a medical examination with a rehabilitation specialist for ten days in September, 1997. The contents of the questionnaire were as follows: general characteristics, physical characteristics, employment status, type of work, working environment and the experience of low back pain. The number of cases with symptoms of occupational low back pain were 321, so the point prevalence was 19.3 persons/100 persons. The number of cases with a history of occupational low back pain for one year were 554, so the one year period prevalence was 33.3 persons/100 persons. The point and one year period prevalences of occupational low back pain showed no significant differences in age, marital status, educational level and body mass index. The point and one year period prevalences of occupational low back pain showed no significant differences in alcohol drinking, smoking, stretching exercise and regular exercise. The point and one year period prevalences of occupational low back pain were significantly higher in the unsatisfied group that the satisfied group (p<0.01). However, no significant differences were found among tenures and shift work. The point and one year period prevalences of occupational low back pain were significantly higher in the lifting of heavy materials group than the nonlifting group(p<0.01). However, no significant differences were found among posture of the waist and the working posture. Through the multivariate logistic regression, significantly associated factors with occupational low back pain were found to be dissatisfaction with job (point prevalence: OR=1.78, 95% CI: 1.21-2.61: one year prevalence : OR=1.76, 95% CI: 1.26-2.47), lifting of heavy materials(point prevalence OR=1.94 95% CI : 1.44-2.61; one year prevalence: OR=2.17, 95% CI : 1.70-2.77) and tenure (point prevalence : OR=1.03, 95% CI : 1.01-1.06; one year prevalence : OR=1.02, 95% CI : 1.00-1.05).